Labshake search
Citations for Thermo Fisher :
2651 - 2700 of 10000+ citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 1 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: Total RNA was extracted from whole head/tail part of the zebrafish body with TRIzol reagent (Invitrogen) following the manufacturer’s instructions and treated with DNase I ...
-
bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood by Ficoll-Paque (GE Healthcare, ThermoFisher) density gradient centrifugation ...
-
bioRxiv - Physiology 2023Quote: ... The whole bladder contractile viability was assessed by application of 3 μM carbachol (L06674, Thermo Fisher Scientific) into the organ bath and washed 3 to 5 times with warm (37°C ...
-
A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: Whole-cell RNA (total RNA) was extracted using TRIzol™ reagent from A549 cells (Thermo Fisher Scientific) according to the manufactureŕs instruction ...
-
bioRxiv - Molecular Biology 2024Quote: Whole-cell extracts (WCE) were prepared by incubating mESC with radioimmunoprecipitation assay (RIPA) buffer (Thermo Fisher, 89900) supplemented with Benzonase (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2024Quote: ... Whole-cell voltage-clamp recordings were made from MBON-γ1pedc using Axon MultiClamp 700B amplifier (Molecular Probes). Cells were held at −60 mV ...
-
bioRxiv - Developmental Biology 2024Quote: ... Whole embryo RNA was obtained from groups of 30 24 hpf embryos using TRIzol (Invitrogen, Cat # 15596026) and Purelink RNA Microkit (Invitrogen ...
-
bioRxiv - Neuroscience 2024Quote: ... Coronal sections (50 μm) of the whole brain were cut using a cryostat (HM525 NX, Thermo Scientific) after being embedded with OCT compound (NEG-50 ...
-
bioRxiv - Developmental Biology 2024Quote: ... the whole-mount or cryosection samples were mounted with Prolong Gold antifade medium (P36930, Thermo Fisher Scientific) at room temperature for 12 h ...
-
bioRxiv - Microbiology 2023Quote: ... Genomic DNAs were extracted from the whole abdomens of individual bees using DNAzol® reagent (Thermo Fisher). We quantified L ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Total RNA was extracted from whole embryos using TRIzol Tri Reagent (Ambion Life Technologies, Waltham, MA, USA), following the manufacturer’s protocol ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: Fresh whole livers rinsed in Dulbecco’s phosphate-buffered saline (DPBS) (Cat# 14190136, Thermo Fisher Scientific, Waltham, MA) were minced to 1 – 3 mm pieces using surgical scissors ...
-
bioRxiv - Genetics 2023Quote: Vibratome-sectioned flies: Whole flies were fixed in 4% PFA and 0.4mg/ml PierceTM DSP (Thermo Scientific) in PBS while rotating for 4-5 hrs at 4°C ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... DNA extraction was performed from whole L4 larvae or pupae using Phire Tissue Direct (ThermoFisher Scientific F170), and paternity was determined by PCR-amplification of the npylr1 locus that differentiates group 1 males (npylr1-/- ...
-
bioRxiv - Molecular Biology 2023Quote: Exosomes along with whole cell pellets were lysed in RIPA Lysis and Extraction Buffer (Thermo Fisher #89900). Protein concentration was determined by a Pierce BCA Protein Assay Kit (Thermo Fisher #23227 ...
-
bioRxiv - Neuroscience 2023Quote: ... Whole cell protein extraction was performed by lysing the cell pellet with Cell Lysis buffer (ThermoFisher, FNN0011) supplemented with protease inhibitor cocktail (ThermoFisher ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Total RNA was extracted from whole embryos using TRIzol Tri Reagent (Ambion Life Technologies, Waltham, MA, USA), following the manufacturer’s protocol ...
-
bioRxiv - Genomics 2022Quote: ... Twenty μg of whole cell lysate were loaded in Novex WedgeWell 4-12% Tris-Glycine Gel (Invitrogen) and separated through gel electrophoresis (SDS-PAGE ...
-
bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from whole embryos or isolated hearts (36hpf and 48hpf, respectively) using Trizol (Invitrogen) and was purified with the RNeasy Micro Kit (Qiagen#74004) ...
-
bioRxiv - Developmental Biology 2023Quote: ... The total RNA of these tissues from the whole transplant are extracted by TRIzol (Invitrogen, CA, USA) for the reverse transcription to total cDNA of plant.
-
bioRxiv - Cell Biology 2024Quote: ... whole cell lysates or immunoprecipitated samples were separated on NuPAGE 4-12% Bis-Tris protein gels (Invitrogen) in MOPS buffer and then transferred to PVDF membrane (Millipore Sigma) ...
-
bioRxiv - Cell Biology 2024Quote: ... Total RNA was extracted from mouse whole liver tissue using Trizol (Life Technologies, Carlsbad, CA, Cat#15596026). The total RNA extracted underwent quality control by Bioanalyzer 2100 (Agilent Technologies ...
-
bioRxiv - Neuroscience 2023Quote: ... 20µl per mouse) and whole brain was dissected and placed in ice-cold Hibernate-A (ThermoFisher Scientific). Dissected brain was minced using scalpels ...
-
bioRxiv - Genomics 2023Quote: ... Whole liver tissue was homogenized using the Polytron PowerGen 500 S1 tissue homogenizer (Fisher Scientific, Hampton, NH) in Radioimmunoprecipitation assay buffer (LifeTechnologies ...
-
bioRxiv - Neuroscience 2024Quote: Fixed whole brain samples were sectioned using a cryostat (Epredia Cryostar NX50, Fisher Scientific, Waltham, MA, US), in which 15 μm slices were processed and mounted directly onto glass slides or submerged in a free-floating cryo-protective solution (30% sucrose ...
-
bioRxiv - Cancer Biology 2024Quote: ... Whole-cell lysates were separated by either 12.5% or 4-12% SDS-PAGE Bis-Tris gels (Invitrogen) and transferred to a 0.45 um PVDF membrane overnight at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... The whole mount prepared cochlear samples were mounted with Prolong Gold antifade medium (P36930, Thermo Fisher Scientific) at room temperature for 1-2 min ...
-
bioRxiv - Molecular Biology 2020Quote: SEC fractions in loading buffer were heated to 95°C for 5 min and separated alongside a spectra multi-color protein ladder (Thermo Fisher 26634) on a 4-15% Tris-Glycine extended Stain-Free gel (Bio-Rad 5678085 ...
-
bioRxiv - Plant Biology 2021Quote: ... Multi-element analysis was conducted using Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) in an iCAPDuo 6500 instrument (Thermo Fisher Scientific), following calibration with a blank and a series of five multi-element standards from single-element standard solutions (AAS Standards ...
-
bioRxiv - Biochemistry 2020Quote: ... Summarized as follows: Samples produced by multi-well screening were run on either an Orbitrap Fusion or a Q Exactive Plus (Thermo Fisher Scientific). Dried peptide samples were resuspended in 10 μL of 5% (v/v ...
-
bioRxiv - Plant Biology 2021Quote: ... The promoter fragment was then assembled with GAL4 into the pB-9FH2A-UAS-7m24GW destination vector in a multi-site gateway reaction to create an activator line construct using the LR Clonase II+ (Thermo Fisher Scientific). This destination vector contains a HISTONE 2A-6 (H2A ...
-
bioRxiv - Plant Biology 2021Quote: ... The promoter and coding sequence fragments were then assembled with GFP into the R4pGWB504 destination vector in a multi-site gateway reaction using the Gateway LR Clonase □ (Thermo Fisher Scientific). To construct the XVE-YUC1 vector ...
-
bioRxiv - Cancer Biology 2020Quote: LNCaP cells were seeded at a density of 5×105 cells into Nunclon D multi-dishes with poly lysine coating (Thermo Fisher Scientific), with or without transfection ...
-
bioRxiv - Cell Biology 2021Quote: ... The channels were then carefully aligned using alignment parameter from control measurements with 0.5 μm diameter multi-spectral fluorescent beads (Invitrogen, Thermo Fisher Scientific). For visualization of the Golgi surface ...
-
bioRxiv - Molecular Biology 2022Quote: ... cross-linked cell lysates prepared by multi-beads shocker (Yasui Kikai Co.) and sonication (Branson) were incubated with Protein G DynabeadsTM (Thermo Fisher, 10004D) attached to ANTI-FLAG® M2 antibody (Sigma-Aldrich ...
-
bioRxiv - Genomics 2022Quote: The genotyping of both RIL population (190 individuals and two parental accessions) and the diversity panel (271 accessions) was performed using the 80K RPGA on GeneTitan® Multi Channel (MC) instrument (Affymetrix, USA) following manufacturer’s instructions (Supplementary Method) ...
-
bioRxiv - Biochemistry 2021Quote: ... were recorded at 30-minute intervals using a Varioskan Lux multi-mode microplate reader equipped with SkanIt 5.0 software (Thermo Scientific, Waltham, MA). Specific fluorescence was determined by dividing the fluorescence by the absorbance to control for population density using the following equation:
-
bioRxiv - Bioengineering 2023Quote: The lungs of mice were homogenized using a Multi-beads shocker (Yasui Kikai, Osaka, Japan) in 9 volumes of Hanks’ Balanced Salt Solution (Gibco #14025-092). Total RNA was extracted from lung homogenates using an RNeasy Mini kit (Qiagen ...
-
bioRxiv - Microbiology 2024Quote: ... GFP images or RFU were collected at indicated time points post-infection by a fluorescence microscope (MI52-N) or a Varioskan LUX Multi-well Luminometer (Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2020Quote: ... Membranes were washed before developed using either the SuperSignal ELISA Femto Substrate (Thermo Fisher Scientific) or ELC Prime Western Blotting system (Sigma-Aldrich ...
-
bioRxiv - Immunology 2021Quote: ... Secreted IL-1β was measured with the Human IL-1β cytoset ELISA (Invitrogen; Cat.# CHC1213) as per the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: Cell culture supernatant was harvested and used to carry out ELISA for IL-1β (Invitrogen) and IL-18 (Invitrogen ...
-
bioRxiv - Immunology 2022Quote: Concentrations of total IgG and IgM in the culture supernatant were determined by ELISA (ThermoFisher) according to the manufacturer’s instruction.
-
bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp ...
-
bioRxiv - Immunology 2021Quote: ... Granzymes A and B secreted into the cell media were measured by ELISA (Abcam, Invitrogen). IELs were analyzed for CD107a externalization through flow cytometry ...
-
bioRxiv - Bioengineering 2021Quote: ... CCL2 was quantified using a rat CCL2 ELISA (Cat. # KRC1012 Life Technologies, Carlsbad, CA, USA) according to manufacturer’s instructions and buffer modifications described in Yarmola et al (29) ...
-
bioRxiv - Immunology 2020Quote: ... Supernatants were assessed for the presence of lupus-associated cytokines by ELISA (Invitrogen / R&D). In analogous cultures of splenocytes ...
-
bioRxiv - Immunology 2020Quote: CRP and IL-6 were measured using a high sensitivity ELISA (Life Technologies, Carlsbad CA). Plasma soluble CD14 (sCD14 ...
-
bioRxiv - Immunology 2020Quote: Cell culture supernatant (diluted ¼) was subjected to ELISA (TNFα, Thermo Scientific, Waltham, MA, n=6) and Luminex (Cytokine Human Magnetic 30 Plex Panel ...
-
bioRxiv - Neuroscience 2022Quote: ... Aggregated Aβ within the soluble fraction was measured using a conformation-specific ELISA assay (Invitrogen).