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Citations for Thermo Fisher :
2651 - 2700 of 8443 citations for IL 12RB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... using the protocol adapted for tissue (KingFisher Duo machine, Thermo Fisher Scientific, Rockford, IL). RNA samples were then quantified (Qubit® 2.0 fluorometer ...
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bioRxiv - Neuroscience 2019Quote: ... Protein signals were detected using a chemiluminescent substrate kit (Thermo Scientific, Rockford, IL USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA samples were then quantified (Qubit® 2.0 fluorometer, Thermo Fisher Scientific, Rockford, IL) and subjected to RNA integrity analysis (2100 Bioanalyzer ...
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bioRxiv - Genomics 2019Quote: ... DNA purity was evaluated with a Nanodrop 2000 spectrophotometer (Thermo Scientific, Hanover Park, IL), quantity with a Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... purified on protein A/G columns (NAb™ Spin Kit, Thermo Scientific, Rockford, IL) following instructions of the manufacturer ...
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bioRxiv - Immunology 2019Quote: ... Ab019 and Ab161 were labelled with Sulfo-NHS-LC-Biotin (Thermo Scientific, IL, USA). The free Sulfo-NHS-LC-Biotin were removed by dialysis ...
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bioRxiv - Cell Biology 2020Quote: ... Detection was further enhanced with an anti-rabbit biotin antibody (Thermo Scientific, Rockford, IL) and AlexaFluor-647 streptavidin-conjugated antibody (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... and protein A/G agarose (#20422) were purchased from Thermo Scientific (Rockford, IL, USA). Recombinant human decorin protein (#143-DE-100 ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were treated with Pierce ECL Western Blotting Substrate (Thermo Scientific, Rockford, IL) and exposed to film.
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bioRxiv - Neuroscience 2020Quote: ... Total proteins were quantified by using BCA kit (Pierce, Thermo Scientific IL 61101 USA). ELISA for IGF-1 (DuoSet ELISA kit ...
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bioRxiv - Developmental Biology 2021Quote: ... 1.25 µL of DMSO (D128-500, Fisher Chemical, Fisher Scientific, Hanover Park, IL, USA), 0.125 µL of Taq (42-800B3 ...
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bioRxiv - Developmental Biology 2021Quote: ... Homemade platinum electrodes (78-0085, Strem Chemicals Inc., Fisher Scientific, Hanover Park, IL, USA) mounted in an Adjustatrode holder (01-925-09 ...
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bioRxiv - Microbiology 2021Quote: ... total RNA was measured using a Nanodrop 1000 Spectrophotometer (Thermo Scientific, Rockford, IL, USA) and was reverse transcribed into cDNAs using oligo-d(T ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined by BCA protein assay kit (Thermo Scientific, Rockford, IL., USA). Equivalent amounts of protein were resolved by 10% SDS-PAGE and transferred to polyvinylidene difluoride membrane ...
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bioRxiv - Immunology 2020Quote: ... in the presence of 5 or 50 ng/ml IL-21 (Thermo Fisher Scientific) as indicated ...
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bioRxiv - Immunology 2020Quote: IL-8 secretion was measured using an ELISA kit (Life Technologies; 88-8086-88). Cell-free supernatant was harvested and stored at −20°C until assayed by ELISA ...
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bioRxiv - Immunology 2020Quote: ... Plate was then washed again and incubated with IL-2 monoclonal antibody (ThermoFisher, USA) for 2 hours at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Sample protein concentrations were measured (Pierce BCA Protein Assay Kit, Thermo Scientific, Rockford, IL), and volumes were adjusted to normalize microgram per microliter protein content ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lysate was prepared with Pierce™ RIPA buffer (89900, Thermo Scientific, Rockford, IL) containing Protease and Phosphatase Inhibitor Cocktail (1861281 ...
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bioRxiv - Pathology 2022Quote: Total protein was quantified using the BCA protein assay kit (Thermo Scientific, Rockford, IL). Western blotting was done as previously described 16 ...
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bioRxiv - Immunology 2022Quote: ... and IL-1β concentrations were measured using a ProcartaPlex simplex assay (ThermoFisher, Waltham, MA).
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bioRxiv - Pathology 2022Quote: ... Primary antibodies were replaced by normal rabbit IgG or mouse IgG (Invitrogen, Rockford IL) and included in each staining series as the negative control ...
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bioRxiv - Neuroscience 2022Quote: ... and gene expression was analyzed using TaqMan probes (ThermoFisher Scientific Inc., Rockford, IL USA) using a ViiATM7 Real Time PCR System or QuantStudio 6 FLEX Real Time PCR System (ThermoFisher Scientific Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... that contains a Halt Protease Inhibitor Cocktail (catalog#1861281, Thermo Scientific, Rockford, IL, USA). Cultured neurons cells were lysed at day 8 of differentiation ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 500 U/ml IL-2 and 1X Glucose Solution (Thermo Fisher Scientific). Cell counts were analyzed every six hours using the Incucyte Live Cell Analysis System (Essen BioScience) ...
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bioRxiv - Microbiology 2024Quote: ... 10% (v/v) heat inactivated fetal calf serum (FCS, Hyclone, Thermo Scientific, Rockford, IL), 100 U/mL penicillin ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were placed on gelatin-coated glass slides (SuperFrost Plus, Thermo Fisher Scientific, IL) and submerged in 0.5% cresyl violet solution (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... we added 0.95 µg of MS grade trypsin (Thermo Scientific Pierce, Rockford, IL, USA) mixed in 40 µL of ABC to each filter and incubated at 37°C for 16 hours ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were treated with Pierce ECL western blotting substrate (Thermo Scientific, Rockford, IL) and exposed to film.
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bioRxiv - Molecular Biology 2024Quote: ... we added 0.95 µg of MS grade trypsin (Thermo Scientific Pierce, Rockford, IL, USA) in 40□µl of 50 mM ammonium bicarbonate to the filters and incubated 13.5 hours in a wet chamber at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... The plasmids were then extracted with GeneJET Plasmid Miniprep Kit (Thermo Scientific, Rockford, IL). The PCR products and plasmid insertions were confirmed without unexpected mutations by using Sanger sequencing.
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bioRxiv - Bioengineering 2023Quote: ... the cells were challenged with 1 ng/mL murine IL-1α (400ML005CF; Fisher Scientific). 500µL of culture media was collected and snap frozen at 0 ...
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bioRxiv - Molecular Biology 2023Quote: ... cell lysis was performed by adding M-PER lysis buffer (Thermo Scientific; Rockford, IL) supplemented with 1x cOmplete Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... Electroporation was performed using the NEON Transfection System (Thermo Fisher Scientific, Rockford, IL, USA), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... The proteins and peptides were visualized using chemiluminescent substrates (Thermo Scientific, Rockford, IL, USA) using a ChemiDoc MP imaging system (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... HALT Protease and Phosphatase Inhibitor Cocktail (1:100, 1861280, Thermo Scientific, Rockford, IL, USA), pepstatin A (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... cells were lysed with RIPA lysis and extraction buffer (ThermoFisher Scientific, Rockford, IL, USA), supplemented with 1x protease inhibitor (Pierce Protease Inhibitor Tablet ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10 % fetal calf serum (FCS, Gibco, ThermoFisher Scientific, Hanover Park, IL, USA). In order to induce the Tripz-construct cells were treated with a final concentration of 5 µg/ml doxycycline hyclate (DOX) ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies: cardiac troponin T mouse monoclonal antibodies (cTnT: Thermo Scientific, Rockford, IL, USA), diluted to 1/200 with 3% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... ELISAs were performed to measure the release of IL-2 (Invitrogen, #88-7025-88), IFN-γ (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Cells were then incubated with IL-17A FITC (clone: eBio17B7, 11-7177-81; Invitrogen), IL-13 PE (clone ...
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bioRxiv - Cancer Biology 2024Quote: ... ECL solution (entry-level peroxidase substrate for enhance chemiluminescence) (Thermo Scientific, Rockford, IL, USA) was loaded on the membrane in order to allow the chemiluminescent reaction between horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2024Quote: ... Pierce bicinchoninic acid (BCA) protein assay kit was purchased from Thermo Scientific (Rockford, IL). Ethyl alcohol (190 proof ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then fixed with 500μL 4% paraformaldehyde (PFA) (Thermo scientific, Rockford, IL, USA) solution in PBS 1X ...
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bioRxiv - Immunology 2024Quote: ... Conventional PCR was performed using DreamTaq Green DNA Polymerase (Thermo Scientific, Rockford, IL, USA), following manufacturer’s indications ...
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bioRxiv - Bioengineering 2024Quote: ... Concentration of IL-12 p70 in serum was determined using mouse ELISA Kit (Invitrogen) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Pierce bicinchoninic acid (BCA) protein assay kit was purchased from Thermo Scientific (Rockford, IL). NuPAGE Bis-Tris precast gels were purchased from Life Technologies (Carlsbad ...
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bioRxiv - Developmental Biology 2021Quote: ... The ORF of red fluorescent protein gene (RFP)-fused Antp was inserted into a pIZ/V5-His vector (Invitrogen) driven by the OpIE2 promoter ...
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bioRxiv - Molecular Biology 2021Quote: ... and the C-terminal foldon trimerization motif followed by an 8×His-tag was cloned into the pcDNA3.1(+) expression vector (Invitrogen). Furthermore ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged Gβγ was expressed in Sf9 cells for 2 days following baculovirus infection (pFastBacDual, Bac-to-Bac, Invitrogen). Cells were harvested by centrifugation and lysed by sonication in 20 mM Tris-HCl pH 8.0 ...