Labshake search
Citations for Thermo Fisher :
2651 - 2700 of 10000+ citations for 6 Chloro N 5 ethoxy 1H pyrazol 3 yl 3 nitropyridin 2 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... cDNA was generated from plasma RNA using the primer YB383 5’-TTTTTTTTTTTTTTTTTTTTTTTTRAAGCAC-3’ as per the manufacturer’s protocol for the Superscript III Reverse Transcriptase (Thermo Fisher Scientific). cDNA was additionally treated with Ribonuclease H (Thermo Fisher ...
-
bioRxiv - Immunology 2020Quote: ... and 5’-AATGGGAAGTCACGAAGGTG-3’(reverse) and quantitative real-time RT–PCR was conducted on a StepOne Plus PCR instrument (Applied Biosystems). The NF-κB-luciferase (Luc2p ...
-
bioRxiv - Genomics 2020Quote: ... 5′-CAGACTCAACCTCCTCAGG-3′-R) in a total volume of 20 µl of Luminaris HiGreen High ROX qPCR Master Mix (Thermo Scientific) in a real-time thermal cycler STEPOnePlus (A&B Applied Biosystems ...
-
bioRxiv - Cancer Biology 2021Quote: ... Tissue sections and cultured cells were blocked with 3% bovine serum albumin (BSA, Fisher Bioreagents) and 5% normal goat serum (NGS, Gibco #PCN5000) in PBS for 1 hour at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... inv474 (PDB: 1CWV) (in 5 3 direction) and flanked by SacI and HindIII restriction sites was synthetized and subcloned by Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2022Quote: ... 700 μg of protein were mixed and rotated with 0.5 μg of STING antibody for 3 h at 4 °C after which 25 μL of Dynabeads Protein G (Invitrogen, 10004D) were added to each sample and rotated for an additional hour ...
-
bioRxiv - Molecular Biology 2022Quote: ... Fractions with the greatest quantity and purity of UBC9 protein combined and concentrated down to 5 ml using 3-kDa MWCO centrifugal concentrator (Thermo Scientific) at 4,000xg/4 °C ...
-
bioRxiv - Microbiology 2022Quote: ... These RNAs were 35 nucleotides long and they were prepared by in vitro transcription using a DNA template (5’-CAGTAATACGACTCACTATAGGGGAAGCGGGCATGCGGCCAGCCATAGCCGATCA-3’) and the TranscriptAid T7 high-yield transcription kit (Thermo Scientific). The reaction was performed in a 50-μl mixture containing 1× TranscriptAid reaction buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... The cells were then washed 3×5 min with PBS and mounted between a microscope slide and a coverslip using ProLong™ Gold Antifade Mountant (Invitrogen). The samples were cured at RT overnight and then stored at 4°C until imaged ...
-
bioRxiv - Neuroscience 2024Quote: ... 3-5 mm skin biopsies were collected in Biopsy Collection Medium (RPMI 1460 [Thermo Fisher] with 1X Antibiotic-Antimycotic [Thermo Fisher]), cut into smaller pieces (<1 mm ...
-
bioRxiv - Physiology 2024Quote: ... plus 1% penicillin/streptomycin in a humidified incubator at 37 °C and 5% CO2 and passaged every 3-4 days using 0.05% of Trypsin-EDTA (Thermo Fisher Scientific) to lift the cells from the culture dish ...
-
bioRxiv - Microbiology 2024Quote: ... cells were washed and fed with the complete Lifeline medium plus antibiotics and transfected with 50 nM custom mirVana miRNA inhibitor (inhibitor sequence: 5′-GAGCCCGACGAGCUACCAGACUGCUCCA-3′, #4464086, ThermoFisher Scientific) or 50 nM mirVanna inhibitor negative control#1 (#4464077 ...
-
bioRxiv - Biophysics 2024Quote: ... Texas Red-DHPE (Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt), NeutrAvidin, and Zeba spin desalting columns (7K MWCO, 5 mL) were purchased from Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... The purified RNAs were reverse transcribed using the DP3 primer (5’- CCTTGGCACCCGAGAATTCCA-3’, 0.5 μM) and Superscript III reverse transcriptase (Invitrogen, 18080-044) and treated with Exonuclease I (NEB ...
-
bioRxiv - Neuroscience 2023Quote: ... Heads were washed with PAT for 5 hours and then incubated for 3 days in PAT-NGS with goat anti-rabbit AlexaFluor-488 (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10μg of total protein were heat-denatured for 5 minutes at 100°C before loading onto NuPAGE 3– 8% TRIS Acetate Midi Gel (Novex, Life Technologies) and transferred to PVDF membranes (Millipore) ...
-
bioRxiv - Microbiology 2022Quote: ... Eluted vRNA was subsequently used as a template for cDNA synthesis and reverse-transcribed using the reverse primer SHIV.Env.R1 (5’-TACCCCTACCAAGTCATCA-3’) and SuperScript III reverse transcriptase (Thermo Fisher Scientific #18080044). cDNA was serially diluted to identify the dilution at which <30% of wells contained PCR amplicons of the correct size ...
-
bioRxiv - Biochemistry 2023Quote: ... Total RNA was extracted from individual tissues or pools of 3–5 midguts using TRIzol reagent (Invitrogen, Carlsbad, CA, United States) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: Tubular scaffolds were sterilized in a laminar flow cabinet by washing 3 times for 15 minutes in 5% penicillin/streptomycin (v/v, Gibco, Thermofisher Scientific) in phosphate buffered saline (PBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... at 30°C to an optical density at 600 nm of 3–5 and were harvested by centrifugation at 4000 rpm (Sorvall GS3 or SLA-3000, Thermo Scientific) for 8 min ...
-
bioRxiv - Genetics 2023Quote: ... Ovaries were washed 3 times for 5 min with PBTx before incubation at room temperature in 1 μg/mL DAPI (Invitrogen, ThermoFisher Scientific) solution in PBS for 30 minutes ...
-
bioRxiv - Developmental Biology 2023Quote: ... and sequenced with the M13 forward primer (5’-GTAAAACGACGGCCAG-3’) using a BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific).
-
bioRxiv - Cancer Biology 2023Quote: ... Both the nuclear and chromatin pellet fractions were sonicated 3 times (5 seconds on, 20 seconds off; 30% amplitude; Sonic Dismembranator Model 500 (Fisher Scientific)) on ice ...
-
bioRxiv - Immunology 2023Quote: ... followed by first strand cDNA synthesis using either a NSP4 gene-specific forward primer with a random nucleotide tag sequence (5’-cggtcatggtggcgaataaGCGGCCTTTAATGTGGAATG-3) or without any primer according to the SuperScriptTM III Reverse Transcriptase protocol (Invitrogen, 18080044). cDNA synthesis was then completed followed by a PCR using an eGFP gene-specific reverse primer (5’-CACCTTGATGCCGTTCTTCT-3’ ...
-
bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
-
bioRxiv - Cell Biology 2023Quote: ... KIF18A siRNA used was a 1:1 mixture of the following two Silencer Select Validated siRNAs (5’ to 3’ sequence): GCUGGAUUUCAUAAAGUGGtt (Ambion, AM51334) CGUUAACUGCAGACGUAAAtt (Ambion ...
-
bioRxiv - Cell Biology 2023Quote: ... maintained at 37° C and 5% CO2 and passaged every 3-4 days using trypsin-EDTA 0.05% (Life Technologies, Paisley, UK) to detach the cells ...
-
bioRxiv - Neuroscience 2023Quote: ... 5’-GGAGCCATGGCCGCGTCCGG-3’ and 5’-GGACGGGCGGTCGGTCTGAG-3’ were chosen as Cas9 targeted guide RNAs (sgRNAs) and in vitro transcribed using the MEGAshortscript Kit (ThermoFisher, USA) and subsequently purified using MEGAclearTM Kit ...
-
bioRxiv - Cancer Biology 2023Quote: ... KIF18A siRNAs used were a 1:1 mixture of two the following Silencer or Silencer Select Validated siRNAs (5’ to 3’ sequence): GCUGGAUUUCAUAAAGUGGtt (Ambion, AM51334), GCUUGUUCCAGAAUCGAGAtt (Ambion ...
-
bioRxiv - Systems Biology 2023Quote: ... Cells were washed 3 times for 5 min with PBS and incubated with Image-iT FX Signal Enhancer (Thermo Fisher Scientific) for 30 min at RT ...
-
bioRxiv - Cancer Biology 2023Quote: ... slides or coverslips were washed 3 times for 5 min in PBS and incubated with appropriate species-specific Alexa Fluor-conjugated secondary antibodies (Molecular Probes). DNA was counterstained with Hoechst 33342 (Invitrogen) ...
-
bioRxiv - Cell Biology 2024Quote: ... G cap on its 5’ terminal and poly (A) tail on its 3’ terminal using the mMESSAGE mMACHINE T7 Ultra Kit (Life Technologies). Both the Cas9 mRNA and the sgRNAs were purified using the MEGAclear Kit (Life Technologies ...
-
bioRxiv - Cancer Biology 2024Quote: ... KHDRBS3 knockdown was performed by transfection of 50 nM of non-targeting siRNA (si-CTL) or specific siRNA targeting KHDRBS3 (si-KHDRBS3: 5′ GCGAAGUACUUCCAUCUCAAUGA-3’) (Dharmacon) with Lipofectamine RNAiMAX Transfection Reagent (Invitrogen, #13778075) following manufacturer’s instructions ...
-
bioRxiv - Biophysics 2024Quote: ... against PIEZO1 that targets the 5’-AGCATTGAAGCGTAACAGGG-PAM-3’ at Chr.8: 122513787 - 122513809 on GRCm38 was purchased from Invitrogen (A35533). The cells were transfected with the Cas9 enzyme (TrueCut Cas9 Protein v2 ...
-
bioRxiv - Cell Biology 2024Quote: ... mouse calvariae were dissected from 1–3 days old neonatal mice and digested sequentially 5 times for 25 minutes in α-MEM (Gibco) containing 0.1% collagenase (Roche ...
-
bioRxiv - Neuroscience 2024Quote: Mouse bone marrow-derived macrophages (BMDMs) were collected from the femur and tibia from 3- to 5-month-old mice and grown in DMEM (Gibco, USA) supplemented with 10% fetal bovine serum (Gibco ...
-
bioRxiv - Cancer Biology 2024Quote: ... slides were washed with PBS (3 times x 5 minutes) and mounted using Fluoromount-G (Thermo Fisher Scientific, #00-4958-02).
-
bioRxiv - Bioengineering 2024Quote: ... and incubated for 3-5 minutes at 37°C with a 1:10 dilution of trypsin 2.5% (Thermo Fisher #15090-046) in PBS (HSCs ...
-
bioRxiv - Immunology 2024Quote: ... Slides were washed extensively (3 x 5 mins) in TBS-tween20 and incubated in appropriately labeled secondary antibodies (Invitrogen Carlsbad, CA) for 1 h at RT ...
-
bioRxiv - Immunology 2020Quote: ... 2-mercaptoethanol (5 µM, Gibco) and 150 IU/ml human rIL-2 and 50ng/ml rIL-15) ...
-
bioRxiv - Cell Biology 2020Quote: ... and 2 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific, 11916621) for 1 hour ...
-
bioRxiv - Microbiology 2021Quote: ... adding 2 μg/mL DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific) and 1 μM QUMA-1 23 to the final wash ...
-
bioRxiv - Cancer Biology 2019Quote: ... and 5-6 pieces per well were cultured in a 6-well culture plate (Fisher Scientific). For up to two weeks tissue pieces were cultured in Advanced Dulbecco’s modified Eagle’s medium (DMEM ...
-
bioRxiv - Plant Biology 2020Quote: ... on a QuantStudio 3 real-time PCR instrument (ThermoFisher). Values were normalized to the housekeeping gene SAND (At2g28390) ...
-
bioRxiv - Cell Biology 2020Quote: ... separated on a 3-8 % Tris-Acetate gel (Invitrogen) by SDS-PAGE ...
-
bioRxiv - Cancer Biology 2020Quote: ... and PC-3 cells were cultured in RPMI1640 (Gibco) with 10% FBS and 1% Antibiotic-Antimycotic ...
-
bioRxiv - Microbiology 2020Quote: ... epidermidis (Thermo scientific; 3 μg/ ml in 1% BSA). MSC’s were labelled with anti-vimentin monoclonal antibody (Sigma ...
-
bioRxiv - Developmental Biology 2021Quote: ... counterstained with DAPI or TO-PRO-3 (Thermo Fisher), and coverslipped with Fluoromount-G mounting medium (Southern Biotech ...
-
bioRxiv - Developmental Biology 2021Quote: ... anti–glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Thermo Fisher Scientific), anti-FLAG (Sigma-Aldrich) ...
-
bioRxiv - Developmental Biology 2021Quote: ... 1.1ul (4U) FastDigest-HindIII (prediluted 1/3) (Thermo Fisher) and 11.1ul 20X primer-probe mix ...