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Citations for Thermo Fisher :
2601 - 2650 of 5196 citations for Tryptase gamma Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... secondary antibody at 1:1,000 in blocking buffer along with 1 μg/ml Hoechst 33342 (Thermo Fisher Scientific). After washing three times with PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue sections were blocked for 30 min in a blocking buffer (containing 5% horse serum (Thermo Fisher Scientific) and 1% Triton X-100 in PBS) ...
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bioRxiv - Microbiology 2023Quote: ... The plate was washed with PBS-Tween20 and blocked with SuperBlock™ Blocking Buffer (Thermo Scientific; catalog #37515), and bulk IgG was added ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated in blocking solution with goat or donkey raised Alexa Fluor–conjugated secondary antibodies (Thermo Fisher Scientific) at room temperature for 2 hours ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked for 2 hours at room temperature with SuperBlock T20 blocking buffer (Thermo Fisher Scientific, 37536) and incubated with primary antibodies at a 1:1000 dilution (anti-P-Smad1/5 [clone 41D10] ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed twice in 2x SSC and three times in 1x TBS pH 7.5 before blocking them for 1.5 hours in 10% fetal bovine serum (Gibco)/0.1% Triton X-100/1x TBS at RT ...
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bioRxiv - Genetics 2023Quote: ... Cells were then blocked with 2 mL blocking solution made of 5% Normal Goat Serum (cat#PCN5000 Thermofisher) and 0.05% Triton X-100 in PBS for 2 hours at RT or 4°C on a shaker.
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bioRxiv - Molecular Biology 2022Quote: ... Chambers are blocked in blocking buffer (DPBS, 0.5% BSA, 0.05% Triton X-100, 1:100 SUPERase·InTM (Invitrogen, AM2694)) for one hour at room temperature and YTHDF1 antibody (Abcam ...
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bioRxiv - Immunology 2023Quote: ... After 3 washing steps with PBST and a blocking step using Starting Block PBS (Thermo Fisher Scientific, PI37538) for 1 h at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... and then blocking buffer was added (5% normal goat serum [ThermoFisher], 0.2% Triton™ X-100 [Fisher Scientific] ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then incubated with secondary antibody in blocking buffer containing (5µg/mL) Hoechst nuclear stain (Invitrogen #H3570) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Permeabilized eye cups were incubated in a blocking solution of 10 % normal goat serum (Invitrogen, Camarillo, MD, USA) supplemented with 0.1 % Triton X-100 for 1 h at RT ...
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bioRxiv - Microbiology 2023Quote: ... ab6556)] were used at 1:1,000 in blocking buffer and detected with horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5,000; ThermoFisher). Membranes were washed in TBST followed by addition of enhanced chemiluminescent (ECL ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were rinsed and incubated with blocking buffer containing 10 μg/mL Hoechst blue (Life Technologies, MA, USA) for 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... diluted in blocking buffer: Alexa Fluor 488 donkey anti-mouse IgG (H+L) (1:500, Invitrogen A-21202), Alexa Fluor 488 donkey anti-goat IgG (H+L ...
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bioRxiv - Microbiology 2023Quote: Cells were washed with FACS buffer (0.2% BSA, 1 mM in PBS) before blocking with Fc block (Invitrogen) and incubation with eF780 live/dead viability stain (eBioscience ...
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bioRxiv - Neuroscience 2023Quote: ... For IHC we permeabilized and blocked sections with blocking solution (PBS containing 5% normal goat serum [Thermo Fisher Scientific # 10000C] and 0.1% Triton X-100 [Millipore-Sigma # X100-100ML] ...
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bioRxiv - Microbiology 2023Quote: ... plates were washed 3x with 200μL of PBS containing 0.1% Tween-20 (PBS-T) and blocked with 200μLs of blocking solution (3% goat serum [Gibco, #16210072] ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... After blocking with 10% Normal Goat Serum in PBS pH 7.4 with 0.1% sodium azide (Thermo Fisher Scientific) for 30 min (RT) ...
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bioRxiv - Cell Biology 2024Quote: ... was used for 1h blocking and cells were incubated with anti-myc tag primary antibody (MA1-21316, Invitrogen) diluted 1:500 for 2h in RT ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated in secondary antibody diluted in blocking buffer [goat anti-mouse 488 1:4000 (ThermoFisher Scientific A11029)] for 30 minutes at room temperature with orbital shaking ...
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bioRxiv - Cancer Biology 2024Quote: ... We then incubated membranes with secondary antibody (in 5% Blocking Buffer) for 1 hour at room temperature (Invitrogen cat ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then incubated overnight at 4 ºC in blocking buffer containing 10% Goat Serum (Thermo Fisher) and 0.01% Triton X-100 ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibody solution for BrdU staining: 2 mL blocking buffer containing 20 μL BrdU monoclonal antibody (Invitrogen, B35128). Secondary antibody solution for BrdU staining ...
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bioRxiv - Bioengineering 2024Quote: ... and anti-acetylated tubulin staining: 4 mL blocking buffer containing 8 μL Alexa Fluor 488 (Invitrogen, A-11006), 8 μL Alexa Fluor 594 (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... including 7992 sequences) appended with the iRT peptide sequence using Proteome Discoverer software (version 2.1, Thermo Scientific). The search parameters were set as follows ...
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bioRxiv - Cancer Biology 2021Quote: Peptides were measured on an EASY-nLC 1200 ultra◻high◻pressure system (Thermo Fisher Scientific) coupled to a quadrupole Orbitrap mass spectrometer (Q Exactive HF and HFX and Exploris 480 ...
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bioRxiv - Systems Biology 2020Quote: Peptide extracts (1 μg on column) were separated on an Ultimate 3000 RSLC nano system (Thermo Scientific) using a PepMap C18 EASY-Spray LC column ...
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bioRxiv - Cell Biology 2020Quote: ... for peptide separation coupled online to a Q-Exactive Quadrupole-Orbitrap mass spectrometer (Thermo Scientific, Waltham, Massachusetts) for MS/MS acquisition ...
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bioRxiv - Cell Biology 2020Quote: ... ~4 mg of peptides were enriched for phosphopeptides using a Fe-NTA phosphopeptide enrichment kit (Thermo Scientific) according to instructions provided by the manufacture and desalted ...
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bioRxiv - Developmental Biology 2021Quote: ... peptides were separated using revere phase chromatography on a Dionex Ultimate 3000 RSLCnano UHPLC system (Thermo Scientific) with a C18 trap to Acclaim C18 PepMap RSLC column (Dionex ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The peptide sequences were verified by us using a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific). HEK293 and Sf21 cells expressing FLAG-TRPR were first seeded onto poly-L-lysine-coated 96-well plates and cultured overnight ...
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bioRxiv - Neuroscience 2021Quote: 8 TMT reagents from a 10-plex reagent kit were used to label desalted peptides (Thermo Fisher) as directed by the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluting peptides were on-line sprayed in a LTQ XL mass spectrometer (Thermo Scientific, Sacramento, US). Full scan mass spectra were collected in the linear ion trap in the mass range of m/z 350 to m/z 1800 Da and the 10 most intense precursor ions were selected for collision-induced fragmentation ...
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bioRxiv - Neuroscience 2021Quote: ... and an aqueous phase containing peptides was collected and dried with a SpeedVac (Thermo Fisher Scientific, U.S.A.).
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bioRxiv - Molecular Biology 2021Quote: Eluting peptides were analyzed in data-dependent acquisition mode on a Fusion Lumos mass spectrometer (ThermoFisher Scientific) coupled to the nano-HPLC by a Nano Flex ESI source ...
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bioRxiv - Molecular Biology 2020Quote: ... The tryptic peptides were purified using a 2-µl bed volume of Poros 50 R2 (Applied Biosystems) reversed-phase beads packed in Eppendorf gel-loading tips (Erdjument-Bromage et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The mass spectra were collected using the “Universal” method optimized for peptide analysis provided by Thermo Scientific. Full MS scans (375–1500 m/z range ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were analysed by nanoLC-MS/MS on a Q Exactive HF-X mass spectrometer (Thermo Scientific) as described previously with the following changes ...
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bioRxiv - Cell Biology 2022Quote: ... Pooled samples were fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were analysed by nano-LC MS/MS using an Ultimate 3000 LC system (Dionex, Thermo Scientific) coupled to the triple TOF 5600 mass spectrometer (Sciex) ...
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bioRxiv - Immunology 2021Quote: ... Peptides were trapped on a pre-column (PepMap100 C18 3 μm; 75μm x 2cm, Thermo Fisher Scientific) and separated on an EASY-Spray column (ES803 ...
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bioRxiv - Cell Biology 2019Quote: ... Peptides were loaded onto an Acclaim Pepmap 100 trap column (75 µm ID × 25 cm, Thermo Scientific) for 10 min at 3 µLs/min then eluted onto an Acclaim PepMap RSLC analytical column (75 µm ID × 25 cm ...
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bioRxiv - Microbiology 2019Quote: ... and eluting peptides were analyzed in a QExactive Plus hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific). Each sample was run in technical quadruplicates ...
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bioRxiv - Immunology 2019Quote: ... overnight at 37°C and peptides concentration was checked by spectrophotometry (Nanodrop 2000; ThermoFisher Scientific, Waltham, USA). Then,15 μg of peptides were purified using ZipTipC18 pipette tips according to manufacturer’s instructions (Millipore ...
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bioRxiv - Bioengineering 2019Quote: Peptides were subsequently analyzed by ultra-high-performance liquid chromatography (UHPLC) using an Easy-nLC1200 (Thermo Scientific) online system coupled to a Q Exactive High Field mass spectrometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... Desalted peptides were eluted and directly analyzed by an Orbitrap Discovery mass spectrometer (Thermo Scientific, Waltham, MA). The HPLC system was extensively cleaned between samples ...
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bioRxiv - Biochemistry 2021Quote: Peptide mixtures were trapped on a 2 cm x 100 μm Pepmap C18 column (ThermoFisher Scientific, #164564) and separated on an in-house packed 50 cm x 75 μm capillary column with 1.9 μm Reprosil-Pur C18 beads (Dr ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were directly eluted into a Q Exactive Plus Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific). Data acquisition was performed in data-dependent and positive ion modes ...
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bioRxiv - Cancer Biology 2020Quote: ... LC-MS/MS analysis of the peptides was performed using a Q Exactive mass spectrometer (Thermo Scientific). The peptides were loaded onto a self-packed biphasic C18/SCX MudPIT column using a Helium-pressurized cell (pressure bomb) ...