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Citations for Thermo Fisher :
2601 - 2650 of 7572 citations for Recombinant Human MME Fc Chimera since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 15μL of annealed guide RNA oligos were mixed with 15μL of 1μM recombinant Cas9 (IDT) in 220μL of Opti-MEM media (Gibco) and incubated for 5min at room temperature ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: Recombinant PLD3 and PLD4 proteins were expressed in Expi293F cell line according to the manufacturer’s instructions (ThermoFisher). In brief ...
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bioRxiv - Microbiology 2024Quote: Primary amines of recombinant EtpA were biotinylated with EZ-Link Sulfo-NHS-LC-LC-Biotin (ThermoFisher 21388) for 30 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... Enzymes used for recombinant DNA cloning and Golden Gate Assembly were purchased from Thermo Scientific (Waltham, MA) and New England Biolabs (Ipswich ...
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bioRxiv - Microbiology 2024Quote: His-tagged recombinant full-length SdeC derivatives were adsorbed to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1μg enzyme per 25 μl of packed resin in 1X ART buffer for 1 hr at 4°C with end-over-end rotation ...
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bioRxiv - Microbiology 2024Quote: His-tagged recombinant SdeC and its derivatives were bound to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1 μg enzyme per 25μl of packed resin in 1X ART buffer (20mM Tris 10mM NaCl ...
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bioRxiv - Immunology 2024Quote: ... either the recombinant CT584 or purified Cm EB’S were solubilized in SDS sample buffer (Novex, Life Technologies) in the presence of reducing agent (10mM DTT) ...
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bioRxiv - Microbiology 2019Quote: Human IL-18 and TNFα were measured with the human IL-18 ELISA Kit (MBL, Woburn, Massachusetts) and human TNFα ELISA Kit (ThermoFisher, Waltham, Massachusetts) according to the manufacturers’ instructions using cell culture supernatant at a 1:5 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Human brain samples classified into healthy (n=47) and AD (n=32) were processed by array (Affymetrix Human Gene 1.0 ST Array)53 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Telomerase-immortalized human neonatal foreskin keratinocytes (N-TERTs) (21) were cultured in EpiLife medium containing human keratinocyte growth supplement (HKGS) (Thermo Fisher Scientific) and penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions of human PBMC were stimulated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher Scientific) as per manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... 0.01ug/ml soluble human anti-CD3 (R&D clone UCHT1) and 0.1 ug/ml soluble human anti-CD28 (Life Technologies; clone CD28.2) at a concentration of 1×106 cells/ml in RPMI-1640 (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... SH-SY5Y human neuroblastoma (ECACC) and RH-30 human rhabdomyosarcoma (kindly donated by Marc-David Ruepp) were maintained in DMEM (Thermo Fisher Scientific), supplemented with 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: Cell lines used in this study were THP-1 (human monocytic leukemia) cells, HeLa (Henrietta Lacks, human cervical carcinoma cells, CCL2) and HEK293T Flp-In T-REx 293 (Thermo Fisher Scientific). THP-1 cells were cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Immunology 2021Quote: ... Human primary CD8+ T Cells were negatively isolated from PBMCs using Dynabeads™ Untouched™ Human CD8 T Cells Kit (ThermoFisher Scientific) or Human CD8+ T Cell Isolation Kit (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... and the biotinylated secondary anti-human IgG (ref. B1140-1ML, MilliporeSigma, St. Louis, USA)] and the anti-human IgE (ref. A18803, Invitrogen, Waltham, USA) antibodies were added at 1:1250 and 1:100 dilutions ...
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bioRxiv - Cell Biology 2022Quote: ... were prepared from fresh human umbilical veins as previously described (Barbieri et al., 1981) and maintained in Human Endothelial SFM (Gibco™, Life Technologies Corporation ...
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bioRxiv - Cell Biology 2024Quote: ... Human kidney cells (HKC) and human embryonic kidney cells (HEK293T) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher Scientific, Cat. # 11965126). Mouse embryonic fibroblasts (MEF ...
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bioRxiv - Microbiology 2022Quote: ... 0.01 µg/ml soluble human anti-CD3 (R & D; clone UCHT1) and 0.1 µg/ml soluble human anti-CD28 (Life Technologies; clone CD28.2) for 3 days before infection ...
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bioRxiv - Molecular Biology 2023Quote: ... All hFRG mice reported in this study were engrafted with human and non-human primate hepatocytes from the same donors (Caucasian, 15-month-old donor, Lonza, #HUM181791; ThermoFisher, #CY409, respectively).
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bioRxiv - Immunology 2023Quote: Human pluripotent stem cells (PSCs) were generated from anonymized human PBMCs based on published protocols115 using Sendai viral vectors (Thermo Fisher, A16517) driving high expression of pluripotency markers NANOG and OCT4 ...
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bioRxiv - Biochemistry 2024Quote: ... The preparations of insect cell microsomes containing human CYP2A6 (lot 2520331) and CYP2D6 (lot 2350106) along with human CPR and cytochrome b5 (Baculosomes®) were procured from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Genomics 2024Quote: Dataset 3: This dataset includes the Human Origins SNP Array genotypes (2,583 present-day individuals genotyped on the Affymetrix Human Origins Array) merged with ancient individuals from SW Asia and S Europe called across a total of 616,938 autosomal SNPs (Lazaridis et al. ...
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bioRxiv - Microbiology 2020Quote: 3 ⨯106 cells were washed with FACS buffer (0.2% BSA, 1 mM in PBS) before blocking with Fc block (Invitrogen) and incubation with eF780 live/dead viability stain (eBioscience ...
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bioRxiv - Immunology 2019Quote: ... Human alveolar epithelial carcinoma A549 epithelial cells (ATCC_CCL-185) were grown in 10% FCS with 1% L-glutamine in Hams/F-12 media (Gibco).
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bioRxiv - Biochemistry 2020Quote: ... The absorbance of each well at 450 nm was measured using a Multiskan FC microplate photometer (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2019Quote: ... The culture medium was removed and replaced with RPMI 1640 medium with 10% CS-FCS (Gibco® Life Technologies) and cells were incubated with or without 50 nM T3 for another 3 days ...
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bioRxiv - Cancer Biology 2019Quote: ... The culture medium was removed and replaced with RPMI 1640 medium with 10% CS-FCS (Gibco® Life Technologies) and cells were incubated with or without 50 nM T3 for another 3 days ...
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bioRxiv - Neuroscience 2020Quote: ... The amount of ingested blue dye in the supernatant was measured spectrophotometrically at 620 nm (Fisher Scientific Multiskan FC). Sample readings were normalized to the number of flies.
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bioRxiv - Microbiology 2020Quote: rS1-Fc was expressed in Chinese hamster ovary cells (Free Style CHO-S Cells by Thermo Fisher Scientific, # R80007) in CHO-S-SFM media with hypoxanthine and thymidine (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... EVs were resuspended in D-MEM (0 % FCS) + fluorescent Bodipy Phosphocholine analogue at 2 μg/mL (D3793, Thermofisher Scientific) and incubated overnight at 4°C ...
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bioRxiv - Pathology 2021Quote: Cryosections were permeabilized and blocked for 1 h at RT in 0.1% PBTx and 10% fetal calf serum (FCS; Invitrogen), and incubated O/N at 4 °C with the primary antibodies in 0.1% PBTx plus 1% FCS ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% (v/v) heat-inactivated fetal calf serum (FCS) and 1% (v/v) Penicillin-Streptomycin (P/S, 10.000 U/ml, Gibco). Jurkat ...
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bioRxiv - Microbiology 2021Quote: ... or 20% (THP-1) (v/v) heat-inactivated fetal calf serum (FCS) and 1% (v/v) Penicillin-Streptomycin (10.000 U/ml, Gibco). THP-1 monocytes were treated with 100 nM 12-O-tetradecanoylphorbol-13-acetate (TPA ...
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bioRxiv - Cell Biology 2021Quote: Dulbecco’s modified Eagle’s medium (DMEM) and DMEM-F12 containing 10% heat-inactivated fetal calf serum (FCS) (Thermo Fisher Scientific) were used to culture human HEK293T ...
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bioRxiv - Immunology 2022Quote: ... Extracellular antibodies were diluted in FACS buffer (2% FBS and 5mM EDTA in PBS) with Fc Block (Life Technologies) and incubated for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... cell pellet was re-suspended in ice-cold proliferation growth medium (PGM) containing DMEM supplemented with 10% FCS (Invitrogen), 10% horse serum (Invitrogen) ...
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bioRxiv - Physiology 2022Quote: ... and grown for 4 days on untreated tissue culture plates in a humidified incubator with 5% CO2 at 37°C with culture medium (RPMI, 10% heat-inactivated FCS, 50 units/mL penicillin; 50μg/ml streptomycin -GIBCO) supplemented with 50 ng/ml rhM-CSF (Sino Biologicals) ...
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bioRxiv - Microbiology 2022Quote: ... with 7⍰.5% heat-inactivated fetal calf serum (FCS; Life Technologies with 1% penicillin/streptomycin (PS, 5000U.mL−1 and 5000μg.mL−1 respectively; Life Technologies) and supplemented with 1⍰% non-essential amino acids (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 μL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... and cells were stained with Fixable Viability Dye (eBioscience) and primary antibodies in the presence of Fc block in flow buffer (Dulbecco’s phosphate-buffered saline [Gibco] supplemented with 2% fetal calf serum (FCS) ...
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bioRxiv - Molecular Biology 2021Quote: ... Mouse embryonic fibroblasts (MEF) were grown in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% heat-inactivated fetal calf serum (FCS, GIBCO), 10 U/ml penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2021Quote: ... Germany) were cultivated in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FCS, Biochrom or GIBCO), 100 U/ml of penicillin and 0.1 mg/ml of streptomycin (PAN-Biotech) ...
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bioRxiv - Microbiology 2021Quote: ... J/4320/15) and the absorbance at 450 nm was read on a Multiskan FC Microplate Photometer (Thermo Scientific). Titres were determined by curve fitting data to a 4PL curve in GraphPad Prism 9 and accepting only data which produced an R2 value of >0.95 ...
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bioRxiv - Microbiology 2021Quote: ... 30-2003, ATCC) supplemented with 10% heat-inactivated fetal calf serum (FCS, Hyclone, USA) and 1% pen/strep (Invitrogen) at 37°C in a humidified 5% CO2 incubator (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2022Quote: ... These cells were washed with PBS and then resuspended in PBS supplemented with 2% FCS or FBS and 5µM CM-H2DCFDA probe (Invitrogen) and incubated in the dark for 20min at 37°C under 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... with 7 .5% heat-inactivated fetal calf serum (FCS; Life Technologies with 1% penicillin/streptomycin (PS, 5000U.mL−1 and 5000µg.mL−1 respectively; Life Technologies) and supplemented with 1 % non-essential amino acids (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... They were cultured in RPMI 1640 medium supplemented with l-glutamine and 10% FCS (v/v) (Gibco, Massachusetts, USA). Vδ2+ γδ T cell expansion was stimulated using 5 μM zoledronic acid (Actavis ...
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bioRxiv - Cell Biology 2022Quote: ... The absorbance of each well was measured with a microplate reader (Multiskan FC, Thermo Fisher Scientific, Waltham, MA, USA) set to 450 nm ...