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Citations for Thermo Fisher :
2601 - 2650 of 10000+ citations for Mouse Translational Activator Of Cytochrome C Oxidase 1 TACO1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... followed by 40 cycles of 95°C for 15 s and 60°C for 1 min (Applied Biosystems QuantStudio 7 Flex). Primers used for qPCR reactions are listed in Supplementary Table S5 ...
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bioRxiv - Immunology 2022Quote: ... 40 cycles of 15 s at 95°C, and 60°C for 1 min; ABI PRISM 7000 Sequence Detection System; Applied Biosystems). The relative abundance of parasite rRNA ...
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bioRxiv - Molecular Biology 2022Quote: ... 45” at 60 °C and 15” at 95 °C with a final extension of 1’ at 60 °C and 15” at 95 °C in a StepOne Plus real-time PCR cycler (Applied Biosystems). The cycle number for exponential amplification was determined and normalized to DMSO treated samples and visualized with Prism (GraphPad).
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bioRxiv - Cancer Biology 2022Quote: ... followed by 40 cycles of 15 s at 95⍰°C and 1 min at 60⍰°C using SYBER Green PCR Master Mix (Life Technologies) in the 7500HT system (Applied Biosystems) ...
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bioRxiv - Plant Biology 2024Quote: ... followed by dissociation curve analysis ramping from 60°C to 95 °C with a ramp rate of 0.3 °C s-1 on an ABI StepOne Plus RT-qPCR platform (Applied Biosystems, USA). BnaActin was used as housekeeping gene to normalize the data ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR program comprised an initial denaturation for 30 s at 95 °C and amplification by 40 cycles of 15 s at 95 °C and 1 min at 60 °C and was ran in an ABI QuantStudio 7 Real Time PCR machine (Applied Biosystems). Expression values of DIN6 ...
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bioRxiv - Cell Biology 2023Quote: ... that had been coated overnight with 100 μg/ mL poly-D-lysine at 37 °C and for 1 h at 37 °C with 10 μg/mL laminin (Life Technologies). After 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 40 cycles of 95°C for 15 s and 60°C for 1 min (Applied Biosystems QuantStudio 6 Flex). For testing of U7 snRNA level in U7 KD cells ...
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bioRxiv - Biophysics 2023Quote: ... pH 7.9 and 0.2M NaCl were heated for 5 min at 95°C and then cooled to 25°C in 2°C steps with 1 min per step in a thermal cycler (Applied Biosystems). The resulting double-stranded DNA (∼1 μM ...
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bioRxiv - Cancer Biology 2023Quote: ... The qPCR was initiated by 10 min at 95 °C followed by 40 thermal cycles of 15 s at 95 °C and 1 min at 60 °C in a StepOnePlus real-time PCR system (Applied Biosystems). Table 1 lists the sequences of the primers used.
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bioRxiv - Neuroscience 2024Quote: ... and c- MYC using the CTSTM CytoTuneTM-iPS 2.1 Sendai Reprogramming Kit (Thermo Fisher). The manufacturer’s instructions were strictly followed (CTSTM CytoTuneTM-iPS 2.1 Sendai Reprogramming Kit User Guide).
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated and purified from cultured mouse TPCs (passage 1) with Trizol followed by complementary DNA synthesis with RevertAid™ H Minus kit (#K1632 Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... RNA-seq libraries were prepared with 1 ng of total RNA using an Ion AmpliSeq Transcriptome Mouse Gene Expression kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... RNA-seq libraries were prepared with 1 ng of total RNA using the Ion AmpliSeq Transcriptome Mouse Gene Expression kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... a Luminex assay using the ProcartaPlex™ Mouse Cytokine & Chemokine Panel 1 36-plex kit (#EPXR360-26092-901, ThermoFisher Scientific, Waltham, MA) was performed according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... mouse Anti-ZO-1 (CatNo.339100, Invitrogen; 1:1000 for WB and 1:200 for IF), rabbit Anti- Bdkrb1 (CatNo.577292 ...
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bioRxiv - Microbiology 2021Quote: ... Evaluation of NF-kBp65 and CREB activation was performed in infected or uninfected monocytes using NF-kB p65 (Total/Phospho) InstantOne™ and CREB (Total/Phospho) Multispecies InstantOne™ ELISA Kits (Thermo Fisher), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Total IgG and IgM from Snca+/+ and Snca-/- were measured by commercially available ELISA kits (Invitrogen, #88-50400-22 and #88-50470-22) according to manufacturer’s protocols ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were lysed and protein normalised following content assessment by the Bradford reagent (Walker and Kruger, 2003) for use in an InstantOne™ total/phosphor multispecies p38 ELISA kit (ThermoFisher Scientific, UK) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... The amount of VEGF contained in the thawed medium was measured using the protocols and reagents from the VEGF Human ELISA Kit (BMS277-2, Thermo Fisher Scientific, USA), and the amounts of PEDF were measured using a Human ELISA Kit (RD191114200R ...
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bioRxiv - Bioengineering 2019Quote: ... the VEGF-A amount in the conditioned medium of day 14 was measured using a VEGF-A Human ELISA Kit (Thermo Fisher Scientific, USA). The assay was performed according to the manufacturer’s protocol and absorbance was read at 450nm ...
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bioRxiv - Bioengineering 2022Quote: Epidermal growth factor receptor (EGFR) protein expression levels on the surface of Gli36-derived EVs were quantified using an EGFR Human ELISA kit (ThermoFisher Scientific, Waltham, MA). EVs were spiked in healthy donor serum at different concentrations ranging from 0 to 1.0E11 particles/mL while maintaining the serum-derived EV concentration at 1.0E9 particles/mL ...
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bioRxiv - Microbiology 2023Quote: ... The level of IL-8 in cell culture supernatants was determined using a Human IL-8 Uncoated ELISA kit (Invitrogen, ThermoFisher Scientific Inc.).
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bioRxiv - Zoology 2023Quote: ... Supernatants were collected 24 hours post-treatment and then IL-8 secretion was quantified using commercial human IL-8 ELISA kits (Invitrogen, cat. #88-8086) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... cells were fixed with 4 % paraformaldehyde and stained for β3-tubulin (anti-mouse IgG, 1:500, Sigma followed by Alexa 488 anti-mouse, 1:500, ThermoFisher Scientific and DAPI). Similarly ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunolabeling with performed with mouse anti-B23 primary antibody (1:300; 2h) then detected with Alexa 488 donkey anti-mouse antibody (Invitrogen; 1:500; 1h), then further fixed with 4% paraformaldehyde in DEPC-PBS ...
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bioRxiv - Bioengineering 2021Quote: ... and Tau (mouse monoclonal, Thermo Fisher, AHB0042, dilution 1:1000; secondary antibody: goat anti-mouse Alexa Fluor 647, Life Technologies, dilution 1:1000).Image acquisition was done with an AxiObserver A1 Microscope (Zeiss ...
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bioRxiv - Cell Biology 2023Quote: ... 1:500), anti-mouse IgG2b Alexa Fluor 488 (In-vitrogen A21141, 1:500) and anti-mouse IgG1 Alexa Fluor 594 (Invitrogen A21125, 1:500). The following antibod-ies were used for western blots of human cell extracts ...
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bioRxiv - Pathology 2023Quote: ... and then with the appropriate secondary antibody (Alex Fluor 488/555/647 anti-rabbit IgG, anti-mouse IgG1, anti-mouse IgG2a, anti-mouse IgG2b, or anti-goat IgG; 1:1,000, Invitrogen, Grand Island, NY, USA). Nuclei were counterstained with DAPI (#62248 ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-Map2 a,b,c (MA5-12826; Thermo Fisher Scinetific;1:500), anti-Neuron Specific Enolase (NSE ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were precipitated overnight at −70°C in 1 µl Glycoblue (Invitrogen), 0.1 x volume of 3M Acetate (pH 5.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Frosty® Cryo 1°C Freezing Container (Thermo Fisher Scientific, #5100-0001) in a −80°C freezer for 24 hours before being transferred to liquid nitrogen for long-term storage.
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bioRxiv - Cell Biology 2021Quote: ... Frosty® Cryo 1°C Freezing Container (Thermo Fisher Scientific, #5100-0001) in a -80°C freezer for 24 hours before being transferred to liquid nitrogen for long-term storage.
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bioRxiv - Genomics 2022Quote: ... at 37°C for 1 min and basal medium (Gibco, Paisley, UK) with 50% Foetal Bovine Serum (Gemini ...
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bioRxiv - Microbiology 2024Quote: ... for 1 h at 37°C and then with DAPI (Thermo Fisher) for 10 min at RT ...
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bioRxiv - Plant Biology 2023Quote: ... or HRP-conjugated anti-c-myc antibody (1:5,000) (Thermo Fisher Scientific) for 1 hour at room temperature with gentle shaking ...
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bioRxiv - Microbiology 2023Quote: ... Precipitated cDNA was C-tailed using 1 U/μl TdT (Thermo Fisher) in the presence of 1.25 mM CTP and 1 x TdT buffer at 37 °C for 30 min and subsequently inactivated at 70 °C for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... for 1 hour at 37°C and then with DAPI (Thermo Fisher) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... followed by Proteinase K (Thermo Fisher Scientific; #EO0491, 1 hour, 45°C). Inputs were incubated in parallel during the de-crosslinking step ...
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bioRxiv - Biochemistry 2023Quote: ... for 1 hour at 37 °C and ligated into pcDNA3.1(-) zeo (Invitrogen) using the same restriction sites ...
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bioRxiv - Genomics 2024Quote: ... samples were pre-cleared using washed Dynabeads MyOne C-1 beads (Invitrogen) suspended in nuclei lysis buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... 60°C for 1 min using StepOne Plus™ (Applied Biosystems™). The relative quantity (RQ)/ fold change was calculated using the comparative CT method (2-ΔΔCt) ...
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bioRxiv - Neuroscience 2024Quote: ... and 647 (1:500 for all; PIA32773-mouse; A21202-mouse; PIA32731TR-rabbit; PIA32794-rabbit; A21448-sheep, Invitrogen). The sections were examined using an AX-R Confocal Microscope coupled to a fully automated Ti2-E system (Nikon Instruments Inc ...
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bioRxiv - Neuroscience 2021Quote: ... slices were incubated with secondary antibodies in vehicle overnight at 4 °C (Alexa flor 488 goat anti mouse, Invitrogen A11001 ...
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bioRxiv - Immunology 2019Quote: MCA205 mouse sarcoma cell line is cultured at 37°C under 5% CO2 in RPMI 1640 medium (Life Technologies) in the presence of 1% glutamine ...
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bioRxiv - Genetics 2021Quote: ... overnight at 4°C and then for 2 hours with Dynabeads M-280 Sheep Anti-Mouse IgG (Invitrogen 11201D). After 3 800uL washes in LiCl buffer (100mM Tris HCl pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... followed by incubation at 37 °C for 45 min with Alexa Fluor 555 conjugated goat anti-mouse antibody (Invitrogen). Cells were observed under Leica TCS SP8 confocal microscope (Leica).
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bioRxiv - Immunology 2019Quote: ... Hybridization was performed for 2h at 40°C using the following species-specific probes: (Mouse: Cd14 (ThermoFisher, #VB1-3028230), Cd3e (ThermoFisher ...
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Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: ... 2008) overnight at 4°C and then incubated with anti-mouse secondary antibodies labeled with Alexa fluor 488 (Invitrogen) for 1 hour at room temperature and counterstained with 4′,6-diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Cancer Biology 2023Quote: ... and the mouse macrophage cell line RAW 264.7 were cultured at 37°C in 5%CO2 in RPMI-1640 medium (Invitrogen) containing 10% (vol/vol ...