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Citations for Thermo Fisher :
2601 - 2650 of 10000+ citations for Fipronil Sulfone 3 Cyano Pyrazole 3 4 5 13C4 99%; 3 Cyano 5 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Naïve hESCs were routinely passaged as single cells every 3 days at a ratio of 1:3 using TrypLE™ Express (1X) (Gibco, Thermo Fisher Scientific) and plated in fresh media supplemented with 10 μM of Y-27632 (Stemcell Technologies).
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bioRxiv - Molecular Biology 2020Quote: ... 5×107 ESCs (untreated and OHT-treated) were crosslinked in 10 ml 1x PBS with 1% formaldehyde (methanol-free, Thermo Scientific) for 10 min at 25°C and then quenched by addition of 125 mM glycine ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 600 g for 5 minutes post-fixation and permeabilized with 1 mL ice-cold methanol (Fisher Scientific) for 20 minutes at 4C ...
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bioRxiv - Molecular Biology 2023Quote: ... FUCCI positive cells were cultured and washed once with phosphate-buffered saline (PBS, 300 x g / 5 min) and fixed with 4% methanol-free formaldehyde (Thermo Fisher Scientific, 28906) for 20 min at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... resuspended at 5 OD600nm /ml in 100 nM MitoTracker Red CMXRos (Molecular Probes) diluted in phosphate-buffered saline ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 ml round-bottom flask (PYREX) was rinsed with 100 % chloroform (Acros Organics) twice ...
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bioRxiv - Neuroscience 2024Quote: ... 100 μL of 200mg/ml 5-ethynyl-2’-deoxyuridine (EdU; E10187, ThermoFisher Scientific) was administered daily for 2 weeks by intra perennial injections into 4 week ...
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bioRxiv - Microbiology 2022Quote: ... 0.1 ug of library DNA and 5 µl of salmon sperm DNA (ThermoFisher Scientific) (250 µg/ml final concentration ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 5 ug total RNA was separated in 10% polyacrylamide-TBE-UREA gel (ThermoFisher) using formamide loading buffer (Thermofisher ...
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bioRxiv - Immunology 2022Quote: ... 5 ug of plasmid DNA were placed in 250 μL OptiMEM media (Gibco, 31985062), then 7.5 μL of transit-LTI reagent was added to DNA/OptiMEM solution ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and 5 ug of Dextran Alexa 555 were added into nuclease-free water (Invitrogen) to make 10 ul of mixture ...
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bioRxiv - Molecular Biology 2024Quote: ... and PASH-1::GFP was co-IP’d with 5 ug GFP antibody (Invitrogen, A11120) for 90 minutes at 4°C ...
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bioRxiv - Cell Biology 2021Quote: Human LAMP2A was synthesized into pUC57 Plasmid with the sequence: (5’-CAAGTTTGTACAAAAAAGCAGGCTCTCGAGCAatgGTGTGCTTCCGCCTCTTCCCGGTTCCGGGCTCAGGGCTCGTTCTGGTCTGCCTAGTCCTGGGAGCTGTGCGGTCTTATGCATTGGAACTTAATTTGACAGATTCAGAAAATGCCACTTGCCTTTATGCAAAATGGCAGATGAATTTCACAGTACGCTATGAAACTACAAATAAAACTTATAAAACTGTAACCATTTCAGACCATGGCACTGTGACATATAATGGAAGCATTTGTGGGGATGATCAGAATGGTCCCAAAATAGCAGTGCAGTTCGGACCTGGCTTTTCCTGGATTGCGAATTTTACCAAGGCAGCATCTACTTATTCAATTGACAGCGTCTCATTTTCCTACAACACTGGTGATAACACAACATTTCCTGATGCTGAAGATAAAGGAATTCTTACTGTTGATGAACTTTTGGCCATCAGAATTCCATTGAATGACCTTTTTAGATGCAATAGTTTATCAACTTTGGAAAAGAATGATGTTGTCCAACACTACTGGGATGTTCTTGTACAAGCTTTTGTCCAAAATGGCACAGTGAGCACAAATGAGTTCCTGTGTGATAAAGACAAAACTTCAACAGTGGCACCCACCATACACACCACTGTGCCATCTCCTACTACAACACCTACTCCAAAGGAAAAACCAGAAGCTGGAACCTATTCAGTTAATAATGGCAATGATACTTGTCTGCTGGCTACCATGGGGCTGCAGCTGAACATCACTCAGGATAAGGTTGCTTCAGTTATTAACATCAACCCCAATACAACTCACTCCACAGGCAGCTGCCGTTCTCACACTGCTCTACTTAGACTCAATAGCAGCACCATTAAGTATCTAGACTTTGTCTTTGCTGTGAAAAATGAAAACCGATTTTATCTGAAGGAAGTGAACATCAGCATGTATTTGGTTAATGGCTCCGTTTTCAGCATTGCAAATAACAATCTCAGCTACTGGGATGCCCCCCTGGGAAGTTCTTATATGTGCAACAAAGAGCAGACTGTTTCAGTGTCTGGAGCATTTCAGATAAATACCTTTGATCTAAGGGTTCAGCCTTTCAATGTGACACAAGGAAAGTATTCTACAGCTCAAGACTGCAGTGCAGATGACGACAACTTCCTTGTGCCCATAGCGGTGGGAGCTGCCTTGGCAGGAGTACTTATTCTAGTGTTGCTGGCTTATTTTATTGGTCTCAAGCACCATCATGCTGGATATGAGCAATTTTAGGGTACCACCCAGCTTTCTTGTACAAAGTGGACGCGT-3’) Human LAMP2A was subcloned into pLenti6 (Thermo Scientific), a Gateway adapted vector using BP/LR Clonase II (Thermo Scientific) ...
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bioRxiv - Systems Biology 2023Quote: ... and washed again in PBS (3 x 5 min at RT) before being mounted in Prolong Gold (Invitrogen by Thermo Fisher Scientific, Grand Island, NY, USA) and allowed to cure for 48 hours at RT before imaging ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then counterstained with 4 ug/ml Alexa-488 secondary antibody (Invitrogen), as well as 0.005 U/ul Alexa647-conjugated phalloidin (ThermoFisher) ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 5% foetal calf serum (FCS) and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). KC cells (A Culicoides insect derived cell line ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 5% foetal calf serum (FCS) and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). KC cells (A Culicoides insect derived cell line ...
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bioRxiv - Molecular Biology 2020Quote: ... were grown under standard conditions at 37°C at 5% CO2 in DMEM supplemented with penicillin (100 U/ml) and streptomycin (100 mg/ml) (Gibco). To investigate calcification in the GC1 cells ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell line were maintained at 37°C and 5% CO2 in the presence of 100 unit/ml penicillin and 100 μg/ml streptomycin (Gibco) and were routinely tested for mycoplasma using MycoAlert mycoplasma detection kit (Lonza) ...
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Understanding molecular mechanisms and predicting phenotypic effects of pathogenic tubulin mutationsbioRxiv - Genetics 2022Quote: HeLa cells were cultured and maintained in a humidified 5% CO2 atmosphere at 37°C in DMEM supplemented with 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) and 10% Fetal Bovine Serum (Gibco) ...
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bioRxiv - Cancer Biology 2019Quote: All cells are grown at 37°C with 5% of CO2 with 100 UI/mL Penicillin and 100 µg/mL Streptomycin (Gibco). A673/TR/shEF (Carrillo et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... 37°C in a 5% CO2 incubator with filtered DMEM supplemented with antibiotics (100 IU/mL streptomycin, 100 IU/mL penicillin [Gibco, Invitrogen, Paisley ...
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bioRxiv - Molecular Biology 2021Quote: ... 37°C in a 5% CO2 incubator with filtered DMEM supplemented with antibiotics (100 IU/mL streptomycin, 100 IU/mL penicillin [Gibco, Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... anti-GPIX antibodies (5 µg/ml) and AlexaFluor647-labeled human fibrinogen (Life Technologies, 100 µg/ml) were added to WB ...
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bioRxiv - Microbiology 2022Quote: ... 2.5 x 107, or 5 x 107 PFU/mL (MOI of 5, 50, or 100 respectively) in CO2-independent medium (Gibco Life Technologies) supplemented with 0.1% (w/v ...
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bioRxiv - Immunology 2021Quote: ... 5 ng/mL IL-1β and 5 ng/mL IL-23 from Invitrogen (14823163) (Th17.1s ...
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bioRxiv - Microbiology 2020Quote: ... Sections were then stained with DAPI (2 ug/mL) for 5 minutes before mounting with ProLong Diamond antifade mountant (Thermo Fisher).
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bioRxiv - Molecular Biology 2023Quote: ... Lysis buffer (20 mM Tris pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1% Triton X-100, 1 mM DTT, 25 U/ml DNase TURBO, Invitrogen cat#AM2238) had been added to the frozen monolayer ...
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bioRxiv - Cell Biology 2020Quote: An aliquot of each sample equivalent to 3 μg was loaded onto a trap column (Acclaim PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (Acclaim PepMap RSLC column C18 2 μm ...
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bioRxiv - Biochemistry 2021Quote: ... approximately 1 pmol injected onto a Acclaim PepMap 100 guard column (75 μm×2 cm C18, 3 μm particles, 100 Å; Thermo Scientific) and separated on a 50 cm PepMap RSLC C18 (75 μm×50 cm ...
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bioRxiv - Neuroscience 2020Quote: ... An Acclaim PepMap 100 C18 trap column (20 mm × 75 μm, particle size 3 μm, pore size 100 Å, Thermo Fisher Scientific) was used for online desalting and cleanup ...
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bioRxiv - Systems Biology 2020Quote: ... Alkylated proteins were digested by Lys-C (1:100 w/w) (WAKO, Richmond, VA, USA) at 37°C for 3 h and trypsin (1:100 w/w) (Thermo Fisher Scientific) at 37°C for 16 h ...
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bioRxiv - Systems Biology 2019Quote: ... equipped with a reverse phase trap column (Acclaim PepMap 100, 75 µm x 2 cm, 3 µm, 100 Å; Thermo Fisher Scientific) and 50 cm analytical column (EASY-Spray ...
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bioRxiv - Microbiology 2020Quote: ... before peptide separation on a nanoscale PepMap 100 C18 nanoLC column (3 μm, 100 Å, 75 μm i.d. × 50 cm, Thermo Fisher Scientific) at a flow rate of 0.2 μL per min using a 120 min gradient comprising 100 min from 4% to 25% of solvent B and 20 min from 25% to 40% of solvent B (solvent A consisted in 0.1% formic acid in water ...
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bioRxiv - Neuroscience 2022Quote: ... The peptides were trapped on an guard column (Acclaim PepMap 100, 75 µm x 2 cm, nano viper, C18, 3 µm, 100 Å, Thermo Fisher) using solvent A (0.1% Formic acid ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were loaded onto Acclaim PepMap 100 C18 column (75 µm x 2 cm, 3 µm particle size and 100 Å pore size; #164946, ThermoFisher Scientific) and separated on nano-capillary C18 column ...
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bioRxiv - Molecular Biology 2024Quote: ... equipped with a reverse-phase trapping column Acclaim PepMapTM 100 (C18, 75 μm × 20 mm, 3 μm particles, 100 Å; Thermo Scientific). The injected sample analytes were trapped at a flow rate of 2 µl min-1 in 100% of solution A (0.1 % formic acid) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µg of the sample were injected onto an Acclaim PepMap 100 trap column (75 μm × 150 mm, C18, 3 μm, 100 Å, Thermo Scientific). After flushing the trap column for 3 min at 7 µL/min with 100 % Solvent A the sample is separated on a 40 min linear gradient by an EASY-Spray™ HPLC Columns (75 μm × 250 mm ...
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bioRxiv - Cell Biology 2022Quote: ... The LC system was equipped with an Acclaim Pepmap 100 C18 HPLC nanoViper Trap Column (100 Å, 75 μm X 2 cm, 3 μm; ThermoFisher, USA) and an analytical column as above ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides from the samples were automatically loaded into a trap column (Acclaim PepMap 100, 75 μm Å∼ 2 cm, C18, 3 μm, 100 A, Thermo Fisher) and eluted onto a 50 cm analytical column (PepMap RSLC C18 ...
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bioRxiv - Cancer Biology 2023Quote: ... was loaded onto a trap column (Acclaim1 PepMap 100 pre-column, 75 µm, 2 cm, C18, 3 mm, 100 Å, Thermo Scientific) and then separated on an analytical column (EASY-Spray column ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were loaded onto an Acclaim PepMap 100 trap column (75 μm × 2 cm nanoViper, C18, 3 μm 100 Å, ThermoFisher Scientific) at flow rate of 2 μL/min ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were separated by an Acclaim PepMap 100 C18 trap column (75 µm x 2.0 cm, 3 µm, 100 Å, Thermo Fisher Scientific) and an Acclaim PepMap RSLC C18 nano LC column (75 µm x 25 cm ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were separated by an Acclaim PepMap 100 C18 trap column (75 µm x 2.0 cm, 3 µm, 100 Å, Thermo Fisher Scientific) and an Acclaim PepMap RSLC C18 nano LC column (75 µm x 25 cm ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were washed 3 times with PBS and then permeabilized with 0.1% Triton X-100 (Fisher Scientific, BP151)in PBS for 15 minutes shaking at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... a 0.075 × 150 mm-EASY-Spray column (3-μm particle diameter, 100 Å pore size, Thermo Fisher Scientific) with mobile phases of 0.1% formic acid and 0.1% formic acid/80% acetonitrile was used ...
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bioRxiv - Bioengineering 2020Quote: ... and a C18 nanoViper Trap Column (20 mm x 75 μm, 3 μm, 100 Å; Thermo Fisher Scientific), connected to an Orbitrap Fusion Lumos Tribrid Mass Spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peptides were concentrated using a trap column (0.075 × 20 mm, 3 µm, Acclaim PepMap 100 C18, Thermo Scientific) and then separated using a nano capillary column (0.1 × 150 mm ...
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bioRxiv - Cell Biology 2020Quote: ... which was performed using a 1:3 ratio of 100 nmol/L siRNAs with Lipofectamine 2000 reagent (Invitrogen) diluted in OptiMEM (Gibco ...
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bioRxiv - Plant Biology 2020Quote: ... connected to an analytical column (Acclaim PepMan 100, C18 3 μm, 75 μm × 50 cm, Thermo Fisher Scientific). Peptides were eluted in a gradient of 3-30 % acetonitrile in 0.1 % formic acid (solvent B ...