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Citations for Thermo Fisher :
2601 - 2650 of 10000+ citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... the thermal unfolding temperatures of the chimeric antibodies (0.3 mg/mL) were assessed in triplicate using the Protein Thermal Shift Dye Kit (ThermoFisher Scientific). Continuous fluorescence measurements (λex = 580 nm ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with extracellular antibodies followed by intracellular staining of EdU using click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen), according to the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... The Flow Cytometry analysis panel consisted of the following antibodies: Live/dead fixable near-IR Dead cell stain kit L34975 (Invitrogen); 421 Rat Anti-Mouse CD196 (CCR6 ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were transfected into Expi293F cells using an Expifectamine 293 transfection kit (Gibco) in a 2:1 mass ratio following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Fab were generated from monoclonal antibodies using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Fisher). Antibodies were first digested into Fab in a Zeba Desalt Spin Column (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... Levels of active Rac1 and Cdc42 were detected using Rac1 and Cdc42 specific antibodies from the active Rac1/Cdc42 pull-down kit (Cat# 16118/19, ThermoFisher). Levels of GST-Pak1-PBD protein were detected using Ponceau S staininbg of the membrane ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were washed after secondary antibody incubation before protein detection using SuperSignal West Pico Chemiluminescence Detection kit (Thermo Fisher Scientific). Images were acquired with a Bio-Rad Chemidoc XRS+ gel imaging system and analyzed with ImageJ/Fiji software (National Institutes of Health) ...
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bioRxiv - Immunology 2024Quote: ... and immunoprecipitation was performed with 2μg of anti-survivin antibodies (RnD AF886) coupled to the Dynabeads Protein G Immunoprecipitation Kit (10007D, ThermoFisher Scientific), cross-linked with bis(sulfosuccinimidyl)suberate (A39266 ...
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bioRxiv - Microbiology 2024Quote: Plasmids encoding the heavy chain and light chain of the antibody were transfected at an equimolar ratio into Expi293F cells using ExpiFectamine 293 transfection kit (Gibco) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Direct labelling of antibodies was done using a Zenon™ Alexa FluorTM 594 Rabbit IgG Labelling Kit (# Z25307, ThermoFisher Scientific). DAPI was added to the BS in a concentration 1 µg/ml in the last incubation step ...
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bioRxiv - Bioengineering 2023Quote: ... The GDF-15 and GFP antibodies were biotinylated using an EZ-Link Micro NHS-PEG4-Biotinylation Kit from Thermo Fisher Scientific (21955 ...
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bioRxiv - Cancer Biology 2023Quote: ... The concentration of the antibody was estimated using a Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA). Approximately 4 mg of antibody (2 mL of antibody ...
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bioRxiv - Genomics 2023Quote: ... and SXL (1:500, a gift from Fatima Gebauer) antibodies using the Western Breeze kit following the manufacturer’s protocol (ThermoFisher Scientific). We quantified the relative expression of protein for SXL using the gel analysis tool in ImageJ software following the website’s guidelines 90 ...
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bioRxiv - Developmental Biology 2023Quote: For double labeling tissue with two antibodies both raised in rabbit we utilized a Zenon double labeling kit (Z25302, Invitrogen) with rabbit anti-Fnbp1l ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... incubated with Goat Anti-Rabbit IgG secondary antibody poly HRP (1:1, Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently stained with surface antibodies and then either fixed using 1% paraformaldehyde or permeabilized using a FoxP3/Transcription factor fixation/permeabilization kit (ThermoFisher). Samples undergoing permeabilization were then stained with intracellular antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentrations of the conjugated oligonucleotide DNA and antibodies were quantified using a BCA Protein Assay Kit (Thermo Scientific 23225) and Nanodrop (Thermo Scientific).
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bioRxiv - Microbiology 2023Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were transfected into Expi293F cells using an Expifectamine 293 transfection kit (Gibco) in a 2:1 mass ratio following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Antibody units were then calculated in μg/ml from the standard sera which was quantified using antibody quantification kits from Invitrogen for IgG (88–50400 ...
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bioRxiv - Cell Biology 2024Quote: ... Three technical replicates of 100 µg protein from each biological replicate diluted with diluent supplied by the manufacturer were added to the antibody-coated wells (Ferritin Human ELISA Kit, Invitrogen). All the steps were carried out following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Plastic petri dishes (TPP, Switzerland) were incubated in 100 μg/mL rat tail collagen I (Gibco A10483-01) in 0.1% acetic acid at 4°C overnight on a 60 rpm shaker ...
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bioRxiv - Cell Biology 2020Quote: ... Donkey Anti-rat IgG (H+L) Alexa Fluor 488 (Thermo Fisher; Cat# A-21208; used at 1:250), and Goat anti-mouse IgM Antibody Alexa Fluor 488 (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... α3 rat cDNA was ligated into a previously modified Gateway entry vector pENTR1a (Thermo Fisher Scientific, Waltham, MA) between the human ubiquitin C promoter (UbiC ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time PCR was performed using TaqMan Gene Expression Assay for rat β-actin (Actb, Rn00667869_m1, Applied Biosystems), rat Taste receptor type 1 member 3 (Tas1r3 ...
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bioRxiv - Neuroscience 2021Quote: Slices obtained from VGAT-Venus rats were bulk loaded with 50 μM Fura-2,AM (Invitrogen; Carlsbad, CA) 0.2% Pluronic F-127 in our standard extracellular solution but containing 2 mM MgCl2 ...
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bioRxiv - Bioengineering 2020Quote: ... The sections were then washed and incubated with secondary anti-rat Alexa-488 (1:200, ThermoFisher, A-21208) and anti-rabbit Alexa-647 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: Rats received intracranial injections of anterograde axonal transport tracer PHA-L Alexa Fluor 647 conjugate (Invitrogen; Molecular Probes) into the CeA ...
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bioRxiv - Neuroscience 2020Quote: Rats received intracranial injections of anterograde axonal transport tracer PHA-L Alexa Fluor 647 conjugate (Invitrogen; Molecular Probes) into the CeA ...
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bioRxiv - Neuroscience 2020Quote: ... rats instead received intra-NA core Alexa Fluor™ 488 conjugated CTb (Thermo Fisher Cat. No. C-34775). CTb was administered in five injections (50 nL/injection ...
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bioRxiv - Biochemistry 2021Quote: A7r5 (ATCC) rat thoracic artery smooth muscle cells were sub-cultured in Dulbecco’s modified Eagle media (DMEM; Gibco) supplemented with 10% fetal bovine serum and antibiotic-antimycotic (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: A rat beta cell line (INS1E, AdexxBio C0018009) at passage 38–46 was cultured in RPMI (Gibco 21875) supplemented with 5% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... was made for 3D mono- and co-cultures mixing (per 100μl gel) 34μl of 3mg/ml rat-tail collagen I (Gibco), 4μl of 10x DMEM low-glucose (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: The solution for the first (1.5 mg/ml) and second (0.9 mg/ml) layer of rat tail collagen type I (Thermofisher) was prepared following the jelling procedure instructions of the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... Dy-Light 488-conjugated rat anti-mouse GPIbβ Ab (0.15µg/g;Emfret) and Alexa 647-conjugated fibrinogen (5% total fibrinogen; Invitrogen) were administered via a cannula inserted in the jugular vein ...
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bioRxiv - Molecular Biology 2022Quote: ... and chicken anti-rat IgG conjugated to Alexa Fluor 647 (all Molecular Probes, 1:200, Eugene, OR, USA). ProLong Diamond Antifade Mountant with DAPI was used to visualize DNA (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: Rat PC of pancreatic origin were cultured in CMRL 1066 medium with no glutamine (Thermo Fisher Scientific, UK), supplemented with 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... Oral gavage (OG) vaccinated rats received 100μl dose delivered curved 18 gauge feeding needle (Fisher Scientific Cat. NC9349775). MVC control group received 100μl of the same endotoxin free PBS used to dilute the vaccines in the right thigh muscles of the hind limb ...
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bioRxiv - Immunology 2020Quote: ... and stained with 1:100 dilution of Alexa 555 goat anti-rat polyclonal IgG (Invitrogen; CAT # A-21434), and 1:200 dilution of Alexa 488 streptavidin (Invitrogen ...
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bioRxiv - Physiology 2021Quote: Total RNA was isolated from approximately 100 mg rat livers using TRIzol® reagent (Invitrogen, Carlsbad, CA, USA), followed by the digestion of contaminating DNA with DNAse I (amplification grade ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... stable Nluc-rat A3R expressing HEK 293 cell were selected using 600 μg/mL Geneticin (Thermo Fisher Scientific) whereby the media was changed every two days ...
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bioRxiv - Neuroscience 2021Quote: ... CD IGS rat hippocampus (embryonic day 18-19) were quickly dissected and digested in 0.25% trypsin-EDTA (Invitrogen) at 37°C for 8-10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... CD IGS rat hippocampus (embryonic day 18-19) were quickly dissected and digested in 0.25% trypsin-EDTA (Invitrogen) at 37°C for 8-10 minutes ...
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bioRxiv - Neuroscience 2019Quote: For rats injected with cholera toxin subunit B (CTb) conjugated with Alexa Fluor-488 (CTb-488; ThermoFisher Scientific) in the BNST ...
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bioRxiv - Molecular Biology 2020Quote: ... fixed with 4% PFA and incubated with anti-rat AlexaFluor 594 and anti-mouse AlexaFluor 488 (Thermo Fisher) for 1.5 h ...
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bioRxiv - Systems Biology 2020Quote: ... Microinjected organoids and macrophages were resuspended in freshly prepared Rat Tail Collagen type 1 (Thermofisher, 1 mg/ml) and polymerized in a 4-well ...
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bioRxiv - Physiology 2021Quote: ... Purified rat PCPE-1 concentration (ε= 44350 M−1cm−1) was determined using a nanodrop ND-2000 (Thermofisher).
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bioRxiv - Neuroscience 2019Quote: ... hippocampi or cortices from rat embryos were isolated and incubated in chopping solution [Hanks' Balanced Salt Solution (Invitrogen), 10 mM HEPES (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2019Quote: FLAG-tagged human LRRK2 proteins were enriched from injected rat striatum using Protein G-Dynabeads (50 µl; Invitrogen) pre-coupled with mouse anti-FLAG-M2 antibody (5 µg ...
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bioRxiv - Cell Biology 2021Quote: ... a mixture of rat tail type I collagen (8.33%, First Link (UK) Ltd.) and fluorescent Fibrinogen (647 nm, 1.67 %, Invitrogen) dilluted in PBS was incubated at room temperature for 5 min in order to coat the PEG-free PLL with the protein solution ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was hybridized to Affymetrix GeneChip® Rat Genome 230 2.0 Expression Arrays (Affymetrix, Inc., Santa Clara, CA) utilizing the GeneChip (Expression 3’ Amplification One-Cycle Target Labeling and Control Reagents kit according to the manufacturer’s instructions) ...