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Citations for Thermo Fisher :
2601 - 2650 of 10000+ citations for 6 Hydroxy 1H indole 3 carboxylic acid methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... y229Gt (Tg(tnks1bp1:EGFP)) transgenic larvae were exposed to 250 nM tetramethylrhodamine ethyl ester perchlorate (TMRE, Thermo Fisher, Cat# T669) diluted in EM for 25 minutes while wrapped in foil ...
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bioRxiv - Biochemistry 2024Quote: ... LIG1 proteins were then fluorescently labeled using amine reactive NHS ester conjugated Alexa Fluor (AF)488 according to the manufacturer’s instructions (Thermo Fisher). The protein concentrations and labeling efficiency were finally determined by calculating the molar ratio of dye to protein within the solution by measuring absorbance at 280 nm and 488 nm.
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bioRxiv - Immunology 2024Quote: ... T-allo10 cells or control T-allo Teff cells were labeled with CellTrace carboxyfluorescein succinimidyl ester (CFSE; Thermo Fisher Scientific) for 5 to 8 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: Antibody-dependent cellular phagocytosis was assessed by conjugation of concentrated FL-RhCMV to Alexa Fluor 647 using NHS-ester reaction (Invitrogen), which was allowed to proceed in the dark with constant agitation for 1–2 h and then quenched by the addition of pH 8.0 Tris hydrochloric acid ...
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bioRxiv - Cell Biology 2023Quote: ... as per the manufacturer’s protocol) and a fluorescent Alexa Fluor tag (Alexa Fluor® succinimidyl esters, Invitrogen™, Molecular Probes® ...
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bioRxiv - Immunology 2023Quote: ... which was followed by an overnight incubation at 4°C with N-hydroxysuccinimide ester derivatives of AF647 (Thermo Fisher Scientific) present in two-fold molar excess.
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bioRxiv - Immunology 2022Quote: ... MBP peptide NPVVHFFKNIVTPR and influenza hemagglutinin PRFVKQNTLRLAT peptide were labeled with Alexa Fluor 488 (Alexa488) tetrafluorophenyl ester (Invitrogen, Carlsbad, CA) and used as probe peptides for DP4.1 ...
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bioRxiv - Biophysics 2023Quote: Proteins were labelled by using the AF488-NHS ester according to the manufacturer’s protocol and purified from the excess of the dye by Zeba spin columns (Thermo Scientific), dialysis (Pur-A-Lyzer ...
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bioRxiv - Cell Biology 2023Quote: HM sEV uptake by Caco-2 cells was performed after labeling EVs with carboxyfluorescein succinimidyl ester (CFSE; Thermo Fisher Scientific) [29] ...
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bioRxiv - Biochemistry 2023Quote: Porcine brain tubulin was isolated using the high-molarity PIPES procedure as described (Castoldi and Popov, 2003) and then labeled with biotin- or Dylight-405 NHS-ester (Invitrogen) as described (http://mitchison.hms.harvard.edu/files/mitchisonlab/files/labeling_tubul in_and_quantifying_labeling_stoichiometry.pdf) ...
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bioRxiv - Biophysics 2023Quote: ... 26 The αSA140C and αSA18C mutants were conjugated with AlexaFluor 647 and AlexaFluor 568 NHS-esters (Thermo Fisher Scientific, USA) targeting the cysteine residues ...
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bioRxiv - Biophysics 2023Quote: ... Cy3B and AF647 free dyes were prepared by hydrolyzing the corresponding NHS ( N-hydroxysuccimidyl) esters (PA63101, Cytiva, and A200006, Invitrogen) in 0.1 M sodium bicarbonate overnight ...
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bioRxiv - Cell Biology 2023Quote: ... with NaOH) and were loaded with 5 μM Ca2+ dye Fluo-4 acetomethyl ester (Fluo-4 AM) (Invitrogen Life Technologies) for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... with NaOH) and were loaded with 5 μM Ca2+ dye Fluo-4 acetomethyl ester (Fluo-4 AM) (Invitrogen Life Technologies) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: Antibody-dependent cellular phagocytosis was assessed by conjugation of concentrated RhCMV UCD52 to Alexa Fluor 647 using NHS-ester reaction (Invitrogen), which was allowed to proceed in the dark with constant agitation for 1–2 hours ...
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bioRxiv - Microbiology 2023Quote: Cells were resuspended in PBS and incubated at 4 °C for 5 mins with 8 µg ml−1 Alexa Fluor 555 NHS ester (Invitrogen). Cells were then washed in PBS.
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bioRxiv - Neuroscience 2024Quote: α-IgLON5 AABs and pCtrl were conjugated with endosomal-pH sensitive red-fluorescent dye (pHrodoTM iFL Red STP ester, ThermoFisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: WT IL-10 and Fab-IL-10 constructs were fluorescently labeled using Alexa Fluor 647 N-hydroxysuccinimide (NHS) ester (Invitrogen), and unreacted dye was removed with a Zeba Spin Desalting Column (7K MWCO ...
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bioRxiv - Molecular Biology 2023Quote: ... as already described [17] and reseeded onto 0.2 mm bone slices labelled with N-hydroxysuccinimide ester-activated rhodamine fluorescent dye (ThermoFisher Scientific), at a cell density of 1x105 cells/bone slice (Boneslices.com) ...
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bioRxiv - Biochemistry 2024Quote: NHE activity was measured fluorometrically using the intracellularly trapped pH-sensitive dye BCECF (2ʹ,7ʹ-bis-(Carboxyethyl)-5(6ʹ)-carboxyfluorescein Acetoxymethyl Ester) (#B1170; Invitrogen) with the NH4Cl prepulse technique as described previously (Simonin & Fuster ...
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bioRxiv - Cancer Biology 2024Quote: ... we added an aliquot of barcoded reference cells stained with Alexa Fluor 647 Succinimidyl Ester (0.02 μg/μL) (ThermoFisher Scientific) to all samples ...
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bioRxiv - Microbiology 2024Quote: ... 108 CFU of pneumococci were stained in 100 μl PBS containing 10 μg FITC (Thermo) or AF647-NHS ester (Invitrogen) for 30 min at room temperature (RT) ...
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bioRxiv - Biochemistry 2019Quote: ... Solvent A was 0.1% formic acid in water and solvent B was 0.1% formic acid in acetonitrile (Fisher Scientific). The resulting electrospray (source voltage 1,800 V ...
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bioRxiv - Bioengineering 2021Quote: ... a medium-chain fatty acid (Dodecanoic Acid, C12; BODIPY™ 500/510 C1, C12; D3823, Thermo Fisher Scientific Inc.) or a long-chain fatty acid (Hexadecanoic Acid ...
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bioRxiv - Neuroscience 2021Quote: ... 0.8 mM KH2PO4, 20 mM NaHCO3, 1.3 mM L-glutamine, 0.2 mM ascorbic acid, MEM amino acids solution [Gibco; Thermo Fisher, Waltham ...
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bioRxiv - Cancer Biology 2022Quote: ... was used for essential amino acid (EAA) mix and 1X MEM Non-Essential Amino Acids Solution (Gibco, Cat. #11140050) was used for non-essential amino acid (NEAA ...
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bioRxiv - Molecular Biology 2022Quote: ... C18 disk were washed 10 times with LC-MS grade water containing 0.1 % formic acid and subsequently eluted with 80% acetonitrile/0.1% formic acid and dried using a Speedvac (Thermo Scientific). Peptides were quantified with Quantitative Colorimetric Peptide Assay (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... A 300 μL solution of 5% formic acid and 0.2% trifluoroacetic acid (TFA) R2 50 μm Poros (Applied Biosystems) beads slurry in water was added to the gel pieces before returning the samples to the shaker for an additional three hours at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... formic acid and trifluoroacetic acid (TFA) were liquid chromatography-mass spectrometry (LC-MS) grade solvents (Thermo Fisher Scientific, US). Ethanol was analytical grade (Chem-Supply ...
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bioRxiv - Genetics 2024Quote: ... and 1 mM Ethylenediaminetetraacetic acid [EDTA]) containing 0.01% MilliporeSigma™ GelRed™ Nucleic Acid Stain (Fisher Scientific, catalog # SCT123) with electrophoresis performed at 200V for 30 minutes to detect the 604 bp transgene amplicon ...
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bioRxiv - Neuroscience 2024Quote: ... 0.8 mM KH2PO4, 20 mM NaHCO3, 1.3 mM L-glutamine, 0.2 mM ascorbic acid, MEM amino acids solution [Thermo Fisher] ...
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bioRxiv - Immunology 2024Quote: ... and 1 mM Ethylenediaminetetraacetic acid [EDTA]) containing 0.01% MilliporeSigma™ GelRed™ Nucleic Acid Stain (Fisher Scientific, catalog # SCT123) with electrophoresis performed at 65V for 1 hour to detect the 230 bp transgene amplicon ...
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bioRxiv - Microbiology 2022Quote: Nucleic acid extraction was carried out using the MagMAX CORE Nucleic Acid Purification Kit (Applied Biosystems, Thermo Fisher Scientific). A pre-treatment step using bead beating tubes from MagMAX CORE Mechanical Lysis Module was introduced to improve DNA recovery ...
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bioRxiv - Microbiology 2022Quote: Nucleic acid extraction was carried out using the MagMAX CORE Nucleic Acid Purification Kit (Applied Biosystems, Thermo Fisher Scientific). A pre-treatment step using bead beating tubes from MagMAX CORE Mechanical Lysis Module was introduced to improve DNA recovery ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5% acetic acid) and solvent B (80% acetonitrile, 0.5% acetic acid) into an Orbitrap Eclipse mass spectrometer (Thermo Scientific). High resolution full MS spectra were acquired with a resolution of 120,000 ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... amino acids 23-429) or pro- BMP10 (NP_055297.1, amino acids 22-424) was cloned into pcDNA3.1+ (Invitrogen/Thermo Fisher Scientific) downstream of the rat serum albumin signal peptide ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Nvsix3/6:venus was generated by subcloning the Nvsix3/6 coding sequence into pENTR/D TOPO (ThermoFisher Scientific) using published primers previously used to PCR amplify Nvsix3/6 and synthesize Nvsix3/6 mRNA 47 ...
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bioRxiv - Microbiology 2021Quote: ... thaliana seedlings cultured in 6 ml liquid MSgg of each well of a 6-well microplate (Thermo Scientific). 8 seedlings were put in each well ...
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bioRxiv - Microbiology 2020Quote: ... Cells in antibiotic free media (8×105/6-well) were transfected 6 hours post infection with 2μg plasmid and 6μl Lipofectamine 2000 (Invitrogen) per well diluted in Opti-MEM (Invitrogen).
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bioRxiv - Physiology 2023Quote: ... IL-6 and FFA content were measured by ELISA (IL-1β, Biolegend, 432604; IL-6, Invitrogen, BMS603-2) or a FFA fluorometric kit (Cayman Chemical ...
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bioRxiv - Synthetic Biology 2024Quote: ... Jurkat cells and NALM-6 expressing firefly luciferase- GFP (NALM-6) were cultured in RPMI 1640 (Thermo Fisher) supplemented with 10% FBS and 100 units/mL penicillin and streptomycin ...
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bioRxiv - Developmental Biology 2020Quote: ... 6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific). Cells were imaged using a Zeiss Axio fluorescence microscope.
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bioRxiv - Neuroscience 2021Quote: ... with Essential 6 medium (#A1516401; Life Technologies) containing dorsomorphin (2.5 μM ...
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bioRxiv - Molecular Biology 2020Quote: ... Twelve-well Novex 6% Trisglycine gels (Invitrogen) were pre-run in 0.5× Tris-Borate-EDTA (TBE ...
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bioRxiv - Neuroscience 2021Quote: ... 6 diamidino-2-phenylindole dihydrochloride (DAPI; Invitrogen) for 3 min and washed ...
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bioRxiv - Genomics 2020Quote: ... on the QuantStudio 6 Flex (Life Technologies). Next ...
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bioRxiv - Immunology 2021Quote: ... IL-6 mouse ELISA kit (ThermoFisher Scientific) and IL-1β mouse ELISA kit (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... or the QuantStudio 6 Flex (Thermo Fisher). Samples were analyzed using a two-step amplification and melt curves were obtained after 40 cycles ...
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bioRxiv - Genetics 2020Quote: ... or the QuantStudio 6 Flex (Thermo Fisher). The Ct values were analyzed by the enrichment compared to input method.
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bioRxiv - Bioengineering 2019Quote: ... Novex TBE - DNA retardation gels (6%) – (ThermoFisher) were used for the electrophoresis.