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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and SARS-CoV-2 (strain UF-1) viruses were propagated in Vero-E6 cell line (ATCC® CRL-1586™) in advanced DMEM (Gibco™, 12491015; Thermo Fisher) supplemented with 10% HyClone™ Defined low antibody ...
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bioRxiv - Immunology 2021Quote: ... 1:100 human IgG (1 mg/ml) as FcR block and 2 % FCS using the following staining reagents: 7-AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Cell Biology 2021Quote: ... Akata cells were grown at 37°C under 5% CO2 in RPMI-1640 supplemented with 10% FCS and 2 mM of L-Glutamine (Gibco, 25030-081). Cells KD for BHRF1 were generated from Akata cells (see below ...
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bioRxiv - Immunology 2022Quote: ... were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies, 25030-024], 1 mM sodium pyruvate [Gibco Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... spun at 200 × g for 10 min and resuspended in macrophage medium (DMEM [Invitrogen] plus 10% FCS and 2 mM L-glutamine) in order to have 2.5 × 106 cells per well ...
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bioRxiv - Immunology 2020Quote: ... Staining was performed on ice for 25 minutes in PBS with 2 % FCS using the following antibodies: 7- AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% FCS (BioConcept, Cat# 2-01F16-I) and 100 units/ml of penicillin and 100 µg/ml of streptomycin (Invitrogen, Cat# 15140122). Rat primary fibroblasts (REFs ...
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bioRxiv - Immunology 2023Quote: ... Isolated T-cells were resuspended in complete RPMI (RPMI 1640 [Gibco, #21870-076] supplemented with 2% FCS and 100x Penicillin-Streptomycin [Gibco, #10378-016]).
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bioRxiv - Developmental Biology 2023Quote: ... with 10% FCS (Biosera, #FB-1280/500) and 2% glutamate/pyruvate (100mM sodium pyruvate, Invitrogen #11360-039; 200mM L-glutamine, Invitrogen, #25030-024). Slides were imaged for brightfield ...
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bioRxiv - Immunology 2024Quote: ... viremia samples or organ samples in 96-well plates in MEM (Sigma, M0446; supplemented with 2 % FCS and 1 % Penicilline/ Streptomycin (P/S; ThermoFisher, 15140-122)) ...
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bioRxiv - Cell Biology 2020Quote: ... then Alexa Fluor Donkey anti-sheep 594 secondary (1:250—Life Technologies #A-11016), along with DAPI and phalloidin as described above.
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bioRxiv - Immunology 2021Quote: ... hepaticus was inoculated on blood agar plates (TSA with 5% sheep blood, Thermo Fisher) and cultured in a hypoxia chamber (Billups-Rothenberg ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ... GR1 and MAC1 in combination with sheep anti-rat IgG Dynabeads (Thermo Fisher Scientific) prior to the specific antibody staining ...
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bioRxiv - Molecular Biology 2021Quote: ... was conjugated to Dynabeads M-280 sheep anti-mouse IgG beads (Invitrogen, catalog #11201D). For FLAG ChIP-seq (for Brn1 and Hho1) ...
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bioRxiv - Molecular Biology 2020Quote: ... 15 μl Dynabeads M-280 Sheep Anti-Mouse IgG (Invitrogen, Carlsbad, California, United States) per sample were washed twice in 1x BrU-IP buffer (20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Immunology 2022Quote: ... and Lin+ cells were depleted with sheep anti-rat IgG immunomagnetic beads (Dynabeads, Invitrogen). Cells were then immunostained and Lin- Flk2+CD27+ BM progenitors from F1 congenic strains were FACS purified (Fig ...
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bioRxiv - Microbiology 2019Quote: ... United Kingdom) and Alexa Fluor 488 donkey secondary antibody against sheep (#A11015, Life Technologies). Images of in situ PLA and immunofluorescence experiments were taken with a Zeiss Axioskop 40 fluorescence microscope using an AxioCam MRc 3 CCD sensor and 40x or 100x objectives with filters for DAPI ...
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bioRxiv - Genomics 2019Quote: ... Dynabeads M-280 sheep anti-rabbit IgG beads (Thermo Fisher Scientific, Cat. No. 11203D) were used for immunoprecipitation ...
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bioRxiv - Molecular Biology 2019Quote: ... pre-coupled to magnetic beads (Dynabeads M-280 Sheep anti-Rabbit IgG, Thermo Fisher). A series of wash steps was employed to ensure stringency ...
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bioRxiv - Physiology 2021Quote: ... Samples were then incubated with sheep anti-goat Alexa Fluor 594 (Life Technologies, A11058), or goat anti-mouse Alexa Flour 488 (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... or donkey anti-sheep Alexa-Fluor-488-conjugated antibodies were used (Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... supernatants were immunoprecipitated at 4°C overnight with Dynabeads Sheep anti–Mouse IgG (Invitrogen) conjugated with an anti-FLAG antibody (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... supernatants were immunoprecipitated at 4°C overnight with Dynabeads Sheep anti–Rabbite IgG (Invitrogen) conjugated with each antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... Donkey anti-sheep IgG Cross-adsorbed secondary antibody Alexa Fluor 488 (Invitrogen #A-11015) (1:700).
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bioRxiv - Molecular Biology 2022Quote: ... then for 1h at RT with prepared dynabeads sheep anti rabbit IgG (Invitrogen, #11203D). Coprecipiate was washed once with wash buffer (polysomal buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... the section incubated with Rabbit anti-Sheep secondary antibody (Thermofisher, Cat#: PI31240, 1-500), and the signal was detected using Leica Refine RED Kit (LeicaBiosystem ...
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bioRxiv - Cancer Biology 2023Quote: ... Magnetic beads (11204D, Dynabeads M-280 sheep anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific) were pre-blocked with BSA (1 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Magnetic beads (11204D, Dynabeads M-280 sheep anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific) were pre-blocked with BSA (1 mg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa 488-conjugated donkey anti-sheep IgG (1:300, Invitrogen, Carlsbad, CA, USA, A11015), Alexa 488-conjugated donkey anti-mouse IgG (1:300 ...
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bioRxiv - Microbiology 2023Quote: ... solfataricus Alba antiserum as primary antibody with donkey anti-sheep IgG Alexa488 (Thermo Fisher) as secondary antibody ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... or Alexa Fluor 594-labeled donkey anti-sheep IgG (1:200; A11016, Life Technologies) for 2h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... Lauric acid (dodecanoic acid, LA, 99%, Figure S1(c)) was obtained from Acros Organics. The phospholipids DOPC (1,2-dioleyoyl-sn-glycero-3-phosphocholine ...
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bioRxiv - Microbiology 2020Quote: ... siRNA (Table S1) was transfected into C6/36 cells with Cellfectin II reagent (Invitrogen). JEV was inoculated at 24 h post transfection ...
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bioRxiv - Immunology 2022Quote: ... Primary antibody for CD3 (1:200 dilution; Cat# RM-9107-S1, Thermo Fisher Scientific) was incubated for 30m at 4 °C and detection was achieved using UltraView DAB detection kit (Roche) ...
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bioRxiv - Bioengineering 2024Quote: ... Movies S1 and S2 were obtained on an EVOS M5000 epifluorescence microscope (ThermoFisher Scientific). For cardiomyocyte experiments ...
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bioRxiv - Molecular Biology 2021Quote: ... we cloned an EcoCas3 cDNA with a octa-histidine tag and a six asparagine-histidine repeat tag into a pFastbac-1 plasmid (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... ACE2-S-tag expression was detected by using a mouse anti-S-tag monoclonal antibody 6.2 (Invitrogen, Cat. No. MA1-981), and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... cDNA encoding mouse TCR 2B4 α (with c-Myc-tag) and β (with V5-tag) sequences with 2A sequence linking each other were cloned into pCDNA3.1/Zeo(+) vector (Life Technologies) using Not1 and Xho1 restriction enzymes ...
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bioRxiv - Biochemistry 2021Quote: ... Coverslips were washed three time with PBS before the incubation with Alexa Fluor 555 (for Myc tag) and/or Alexa Fluor 647 (for HA tag) conjugated secondary antibodies (Invitrogen/Thermo Fisher) diluted in PBSAT for 30 min at RT ...
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bioRxiv - Immunology 2021Quote: ... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... PR8-HA and SARS-CoV-2 S proteins with C-terminal Avi-tags and His-tags were expressed via transient transfection of Expi293 suspension cultures (Thermo Fisher) and purified by polyhistidine-tag affinity and size exclusion chromatography ...
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bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was digested with SmaI and ligated in frame with SmaI a N-terminal 3xFLAG-tag and a C-terminal 6xhistidine tag into a modified and SmaI-digested pET101/D-TOPO expression vector (Thermo Scientific), yielding pET101-NanH2.
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bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was digested with BsiWI and ligated in frame with a N-terminal 3 x FLAG-tag and a C-terminal 6 × histidine tag into a modified and BsiWI-digested pET101/D-TOPO expression vector (Thermo Scientific), yielding pET101-NanH1.
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for one hour with SULFO-TAG conjugated anti-human IgG for mAbs (MSD) or SULFO-TAG conjugated polyclonal anti-human IgG+A+M (Thermo Fisher) for serum samples ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed in 1 mL PBS and 100 µL of Phos-Tag lysis buffer was added dropwise to the well (Phos-Tag lysis buffer recipe: RIPA (ThermoFisher 89900), 3X Halt protease and phosphatase inhibitor cocktail (ThermoFisher 78444) ...
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bioRxiv - Biophysics 2023Quote: Plasmids encoding and expressing M with an N-terminal 6xHis tag and with and without SUMO tag fusions were designed in VectorNTI software (ThermoFisher Scientific) and synthesized de novo by GENEWIZ/Azenta Gene Synthesis service ...
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bioRxiv - Immunology 2024Quote: ITS61.01 with a C-terminal rhodopsin tag (C9 tag) and untagged ITS103.01 were produced in transfected Expi293 cells (ThermoFisher, cat. A14527) and purified on rProtein A Gravitrap columns (Cytiva Life Sciences ...
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bioRxiv - Synthetic Biology 2022Quote: ... while the perfluorinated ether FC-770 and the perfluorinated amine FC-3283 were obtained from Acros Organics (Geel, Belgium). Ethanol (96% vol. ...
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bioRxiv - Plant Biology 2023Quote: ... endogenous peroxidases were first quenched by incubating the material for 1h in a peroxidase suppressor solution (Thermo Scientific). After six baths of 5min in 1x PBS ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding spike variants were transfected into FreeStyle 293-F cells (Thermo Fisher) in 3 mL of medium and harvested four days after transfection ...