Labshake search
Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for Respiratory syncytial virus RSV fusion protein exodomain human heterodimeric IgG1 Fc tag recombinant antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... Antigen retrieval was performed by steaming in citrate buffer (Thermo Fisher Scientific, Cat# 005000) for 40min ...
-
bioRxiv - Immunology 2020Quote: ... Biotinylated antigens were incubated with premium−grade SA−PE and SA−APC (Molecular Probes) or SA-BV421 and SA-BB660 (Biolegend and BD Horizon ...
-
bioRxiv - Immunology 2020Quote: ... Confocal related reagents included antigen retrieval buffer low pH (cat. 00-4955-58, Invitrogen), donkey serum (cat ...
-
bioRxiv - Immunology 2020Quote: ... Mice were subcutaneously vaccinated with 30 μg OVA antigen in 0.1% LPS alum (Thermofisher) one day after the adoptive transfer of T cells ...
-
Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... and subjected to antigen retrieval with 10 mM citrate buffer pH 6.0 (ThermoFisher Scientific). Tissue sections were blocked with Tris-buffered saline (TBS ...
-
bioRxiv - Immunology 2022Quote: ... antigen was conjugated first with AlexaFluor 647 and AlexaFluor 488 NHS esters (Thermo Fisher) and with BHQ-1 quencher as previously described in(Li et al. ...
-
bioRxiv - Immunology 2022Quote: ... The antigen-antibody reaction was visualized by enhanced chemiluminescence assay (ECL, Thermo Fisher Scientific).
-
bioRxiv - Immunology 2023Quote: ... CH505 T/F antigen was covalently bound to fluorescent NeutrAvidin beads (Invitrogen, Waltham, MA). Rh PGT151 IgG ...
-
bioRxiv - Developmental Biology 2022Quote: ... Heat mediated antigen retrieval was performed (pH6: anti-DYNLL1 (Invitrogen, SD08-04, 1:1500), anti-DYNLL2 (Proteintech ...
-
bioRxiv - Microbiology 2022Quote: ... 72 for antigens including anti- S100A9/calprotectin (clone MAC387; Thermo Fisher Scientific, Waltham, MA), anti- histone H3 (citrullinated at R2+R8+R11 ...
-
bioRxiv - Neuroscience 2023Quote: ... Antibody-antigen complexes were visualised by SuperSignal West Pico Plus chemiluminescent substrate (Thermo Scientific) with an iBright 750 Imaging System (Invitrogen) ...
-
bioRxiv - Cell Biology 2023Quote: ... antigen retrieval was performed and antibodies were diluted in Starting blockTM (Thermo Fisher #37542). Anti-mouse-biotin was used as a secondary antibody ...
-
bioRxiv - Developmental Biology 2023Quote: ... and boiled in IHC antigen retrieval solution (Invitrogen, Waltham, MA, USA Cat# 00-495558) for 10 min in a microwave ...
-
bioRxiv - Biochemistry 2023Quote: Capture antigens (ocrelizumab, dupilumab) were coated on 384 well plates (Nunc Maxisorp MTP, Thermo) at a concentration of 1 µg/mL in PBS ...
-
bioRxiv - Developmental Biology 2023Quote: ... and boiled in IHC antigen retrieval solution (Invitrogen, Waltham, MA, USA, Cat# 00-495558) for 10 min in a microwave ...
-
bioRxiv - Cell Biology 2023Quote: ... and affinity purified using the peptide antigens immobilised on a Sulfolink affinity column (ThermoFisher). Results shown were predominantly obtained using affinity-purified “303” and “304” anti-TbSmee1 antibodies ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... mice were bled and total IgG (mouse and human) was pulled down using protein A beads (ThermoFisher, Catalog # 20333). An in–house sandwich ELISA was used to measure human IgG (control IgG or MS-Hu6 ...
-
Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: cDNAs encoding human full-length of CREB3L3 (NM_032607.2) and green fluorescent protein were cloned into pENTR4 vectors (Life Technologies). In addition ...
-
bioRxiv - Microbiology 2023Quote: ... and cytokine concentrations were quantified with the Human Cytokine 16-Plex Protein Standard (Thermo Fisher Scientific, Waltham, MA, USA). For quantification of BPI in human sera ...
-
bioRxiv - Microbiology 2022Quote: ... acquired MS/MS spectra were searched against a human uniprot protein database in Proteome Discoverer 3.2 (Thermo Fisher Scientific) using Label free Quantification with Minora feature detection ...
-
bioRxiv - Bioengineering 2023Quote: ... The DNA coding for the protein sequences was synthesized using codon-optimization for human host cells (Thermo Fisher Scientific). If the codon optimization for the lysine-stretches resulted in six or more consecutive adenines in the DNA ...
-
bioRxiv - Bioengineering 2023Quote: ... Protein expression was achieved by transient transfection of FreeStyle human embryonic kidney 293 (HEK293-F) cells (Thermo Fisher Scientific). Cells were grown in non-baffled shake flasks (Corning ...
-
bioRxiv - Cancer Biology 2021Quote: ... human (Invitrogen). Cells were cultured in DMEM (Corning Cellgro 10-013-CV ...
-
bioRxiv - Microbiology 2020Quote: ... supernatant was harvested for virus titration and cells were lysed in Trizol (Invitrogen). MHV in the supernatant was quantified by standard plaque assay on L2 cells.
-
bioRxiv - Cell Biology 2020Quote: ... HEK-293T cells used for virus production were cultured in IMDM (Life Technologies) supplemented with 20% IFS ...
-
bioRxiv - Neuroscience 2020Quote: ... Virus-containing supernatant was harvested three days after transfection with Lipofectamine 2000 (Thermofisher). This supernatant was then cleared from cell debris by centrifugation at 3500 rpm for 15 min and filtered through a 0.45 µm filter (Millipore) ...
-
bioRxiv - Neuroscience 2021Quote: ... to wash the virus in Opti-MEM™ Medium (ThermoFisher Scientific, Darmstadt, Germany) and concentrate to a final volume of 150 μl.
-
bioRxiv - Neuroscience 2020Quote: ... They were reprogrammed into iPSCs using the replication incompetent Sendaï virus kit (Invitrogen) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... The virus pellet was then re-suspended in PBS (Thermo Fisher Scientific, 10010015) containing 25 mM HEPES buffer (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... For this the virus was diluted in 3 mL of 199 medium (Gibco). After incubation for 2 h at 37°C the medium was aspirated and 3 mL of fresh DMEM (Gibco ...
-
bioRxiv - Microbiology 2022Quote: ... respectively) and 5 μl of 4X TaqMan Fast Virus 1-step mastermix (ThermoFisher). The qPCR plate was sealed and read in the FAM and HEX channels using a QuantStudio™ 5 Real-Time PCR system (ThermoFisher ...
-
bioRxiv - Microbiology 2021Quote: ... using the Taqman Fast Virus 1-stepMaster Mix enzyme (Thermo Fisher, Waltham, MA). The limit of detection of this assay is estimated to be 200 genome equivalents/ml saliva or swab fluid ...
-
bioRxiv - Neuroscience 2020Quote: ... Cellular reprogramming experiments were conducted using the Sendai virus (CytoTune, Gibco, Life Technologies) carrying the four factors Oct4 ...
-
bioRxiv - Neuroscience 2020Quote: ... Cellular reprogramming experiments were conducted using the Sendai virus (CytoTune, Gibco, Life Technologies) carrying the four factors Oct4 ...
-
bioRxiv - Microbiology 2021Quote: ... or Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 1X TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific; 4444434) for a total reaction volume of 20 μl in 96-well PCR plates (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion) and a mixture of anchored oligo-dT and random decamers (IDT) ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) in a total reaction volume of 10 μl ...
-
Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: ... ten-fold dilutions of virus were prepared in diluent medium (Opti-MEM (Invitrogen) with 2% FBS (Gibco ...
-
bioRxiv - Microbiology 2020Quote: ... The virus was rescued and propagated in T-Rex 293 HEK cells (Invitrogen) which repress antigen expression during virus propagation ...
-
bioRxiv - Genomics 2021Quote: ... hiPSCs were generated from primary fibroblasts using Sendai virus reprogramming kit (ThermoFisher Scientific), yielding 2-3 different clonal lines for each individual ...
-
bioRxiv - Cancer Biology 2022Quote: ... packaging cell line for virus production were cultivated in DMEM high glucose (Gibco), supplemented with 10% heat-inactivated fetal bovine serum (Gibco ...
-
bioRxiv - Microbiology 2022Quote: ... or Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics ...
-
bioRxiv - Cell Biology 2024Quote: ... cDNA was prepared using Moloney Murine Leukemia Virus reverse transcriptase (Thermo Fisher Scientific) with Random Hexamers (Invitrogen ...
-
bioRxiv - Biochemistry 2024Quote: ... cells were given virus production media (DMEM supplemented with 20mM HEPES (Gibco; 15630080) and 30% FBS) ...
-
bioRxiv - Immunology 2024Quote: ... the media was changed to virus production medium containing Opti-MEM I (Gibco) + 5 % FBS + 1:100 Glutamax + 1:100 MEM-NEAA (Gibco ...
-
bioRxiv - Immunology 2023Quote: ... RNA from virus stocks was purified using TRIzol Reagent (Thermo Fisher Scientific, #15596026). Briefly ...
-
bioRxiv - Immunology 2022Quote: ... the master mix used was TaqMan Fast Virus 1-Step Master Mix (ThermoFisher). Manufacturer’s protocol was followed to add the appropriate volumes of master mix ...
-
bioRxiv - Microbiology 2022Quote: Purified virus particles were incubated with different concentrations of BS3 cosslinkers (ThermoFisher Scientific) for 30 min at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... and Toledo virus was conjugated with DMSO-dissolved AF647–N-hydroxysuccinimide ester (Invitrogen) with constant agitation at room temperature for 1 hour ...