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Citations for Thermo Fisher :
2551 - 2600 of 6974 citations for Recombinant Human PRMT3 GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The recombinant antibodies were transiently expressed in both Expi CHO and Expi293 cells according to manufacturer’s protocol (Invitrogen). The supernatants from 1mL transient transfections of the antibodies were purified using the AssayMAP BRAVO platform with 5mL Protein A cartridges (Agilent Technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... alanine derivatives were used in a concentration of 10 mM and RNaseOUT recombinant ribonuclease inhibitor (1 µl) (Invitrogen) was added ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant Endocan and PDGF-BB were labeled with Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher Scientific) and Alexa Fluor™ 647 Microscale Protein Labeling Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with ketamine-xylazine and infected intranasally with 5,000 PFU of the non-recombinant virus or 1,000 PFU of the recombinant virus in 50 μl of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). For antibody treatment ...
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bioRxiv - Microbiology 2024Quote: ... Antibodies and recombinant RBD-hFc proteins were purified using Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424). In general ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant baculoviruses were prepared in the Spodoptera frugiperda (sf9) cells using the Bac-to-Bac system (Life Technologies). HIF2α and BMAL1 were co-expressed in Trichoplusia ni Hive Five cells by infection of 25 ml each of baculoviruses per 1 L of High Five culture ...
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bioRxiv - Immunology 2024Quote: Recombinant rabbit mAbs and Fabs were expressed in HEK293F cells using ExpiFectamine™ 293 Transfection reagent (Gibco, A14525). Human mAbs were expressed in ExpiCHO-S™ cells using an ExpiCHO™ Expression System Kit (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA was in vitro transcribed from the linearized recombinant infectious clone using the mMESSAGE mMACHINE Kit (Ambion) and electroporated into Vero E6 cells ...
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bioRxiv - Genomics 2021Quote: ... Lysates and positive technical controls (Human Universal Reference RNA - uhrRNA Agilent Cat# 740000, Santa Clara, California and Human Brain Total RNA brRNA - ThermoFisher AM7962, Waltham, Massachusetts), as well as no-cell negative controls (1X TempO-Seq lysis buffer alone ...
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bioRxiv - Immunology 2020Quote: The assay to measure total IgG was modified to measure IgG subclass responses at 1/100 including a two-step biotinylated anti-human IgG subclass (mouse anti-human IgG1: HP6069, IgG2: HP6002, IgG3: HP6050 IgG4: HP6023, Thermo fisher Scientific, UK) with a streptavidin-Phycoerythrin tertiary (Thermo fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were run on the Clariom™ S Human Array to measure gene expression at >20,000 genes in the human genome (Affymetrix, Santa Clara, CA). Raw data generated from the arrays were read into R statistical software (version 3.5.1 ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor™ 488-Human Transferrin conjugate (Cat# T13342) and DiI–human LDL conjugate (Cat# L3482) were obtained from Molecular Probes (Eugene, OR). Primer kits and Master Mix for RT-qPCR were purchased from Applied Biosystems (Foster City ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized to non-human primate β-actin (Table S1) or human β-actin (Hs99999903_m1, Applied Biosystems; Thermo Fisher Scientific) and calculated by the ΔΔCt method [46].
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized to non-human primate β-actin (Table S1) or human β-actin (Hs99999903_m1, Applied Biosystems; Thermo Fisher Scientific) and calculated by the ΔΔCt method [46].
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bioRxiv - Immunology 2021Quote: PBMC derived CD4+ T cells were isolated from HIV-infected MACS participants using EasySep™Human CD4+ T Cell Isolation Kit and activated overnight with Human T-Activator CD3/CD28 Dynabeads® (Life Technologies). The next day Dynabeads were separated from the CD4+ T cells by manual dissociation followed by magnet isolation ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher, Waltham, Massachusetts, USA). iPSCs were plated in a dish pre-coated with Matrigel® hESC-Qualified Matrix (Corning ...
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bioRxiv - Cell Biology 2023Quote: We profiled the expression of transcripts in human aortic vascular smooth muscle cells using the Clariom D human DNA microarray (Thermo Fisher Scientific Inc.). This platform includes information from probe sets representing known and predicted exons and introns on both strands of the genome that have been mapped to more than genes ...
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bioRxiv - Immunology 2023Quote: Customized cytokine 25-Plex Human ProcartaPlex Assay (customized from Cytokine 25-Plex Human ProcartaPlex Panel 1B with the catalog # EPX250-12166-901, Thermo Fisher Scientific, USA) was used to measure multiple cytokines in the supernatants of stimulated human PBMCs.
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bioRxiv - Cell Biology 2020Quote: ... Established BMEC lines were transduced with GFP-tagged RNAi lentivirus (BLOCK-iT HiPerform Lentiviral Pol II miR RNAi Expression system, Invitrogen Cat# K4934-00) targeting Raptor or Rictor ...
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bioRxiv - Molecular Biology 2021Quote: ... coupled to a Q Exactive hybrid mass spectrometer (Thermo) (N-terminal FLAG-tagged datasets) or an Orbitrap Fusion Lumos Tribrid (Thermo Fisher Scientific, Waltham, MA) mass spectrometer (C-terminal FLAG-tagged datasets) ...
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bioRxiv - Developmental Biology 2019Quote: ... C- and N-terminal GFP-tagged Nubp1 and Nubp2 expression plasmids [32] were transfected using Lipofectamine® 3000 (Thermo Fisher Scientific, Waltham, MA, USA) and imaged using a Nikon C2 Confocal microscope.
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bioRxiv - Biochemistry 2019Quote: ... Site-directed mutagenesis of plasmids to generate the C-terminal Myc tagged ECH1 catalytic mutants and the C-terminal V5 tagged UCP3 truncation mutants was carried out with Platinum pFX DNA polymerase from (Life Technologies, Grand Island, NY), following the manufacturer’s protocols for site-directed mutagenesis ...
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bioRxiv - Genomics 2022Quote: ... we performed end repair and then selectively purified biotin-tagged DNA using streptavidin coated beads (DYNAL™ MyOne™ Dynabeads™ Streptavidin C1, Invitrogen 65001). A-tailing and Illumina TruSeq adapter ligation (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids encoding fluorescently tagged mutant CMV 2b proteins were generated using Gateway® cloning following the protocol provided (Invitrogen, Thermo-Fisher Scientific, Paisley UK) with attB-flanked DNA fragments cloned into the pDONR221 donor plasmid ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids encoding fluorescently tagged mutant CMV 2b proteins were generated using Gateway® cloning following the protocol provided (Invitrogen, Thermo-Fisher Scientific, Paisley UK) with attB-flanked DNA fragments cloned into the pDONR221 donor plasmid ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were further incubated for 2 h at room temperature with HRP-tagged secondary antibody of goat anti-mouse (1:1000) (Life Technologies, Thermo Scientific, USA). The sections were then washed with TBST three times ...
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bioRxiv - Cell Biology 2023Quote: Flag-tagged Parkin was inserted into pMSCV vector by using the pCR®8/GW/TOPO® TA Cloning® Kit (Thermo Fisher Scientific). To perform the pCR®8/GW/TOPO® cloning ...
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bioRxiv - Genomics 2022Quote: ... the HA-tagged protein of interest was detected with 1:500 diluted HA Tag Alexa Fluor 647 conjugated mouse antibody (ThermoFisher, Catalog no. 26183-A647). After antibody incubation ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor™ 488-tagged anti-SUMO2/3 antibody was labelled using an Alexa Fluor® 488 Antibody Labeling Kit (Invitrogen-ThermoFisher, Waltham, MA). Unless otherwise specified ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor™ 488-tagged anti-SUMO2/3 antibody was labelled using an Alexa Fluor® 488 Antibody Labeling Kit (Invitrogen-ThermoFisher, Waltham, MA). Unless otherwise specified ...
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bioRxiv - Biophysics 2023Quote: ... Total tubulin was labeled using a primary antibody against alpha-tubulin and a Cy3-tagged secondary antibody (Thermo Fisher Scientific Inc., Waltham, MA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... Microarray was performed by the UVA DNA Sciences core using the Human Affymetrix GeneChip Array ([HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array). Gene lists were generated from microarray data corresponding to genes >1.25 LogFC and <0.75 logFC were used in downstream analyses ...
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bioRxiv - Microbiology 2022Quote: Cytokines in the cell culture supernatants were measured using the human cytokine storm 21-plex procartaplex panel human magnetic bead Luminex assay (Thermo Fisher, EPX210-15850-901), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Concentrations of FGF19 in human serum samples were tested using a commercial Human Fibroblast Growth Factor 19 Assay Kit (Thermo scientific, Waltham, MA, USA). The level of serum C4 was quantified by a mass spectrometry-based method developed by our group.32
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bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... Two control samples from the same pool of total human brain RNA (Ambion FirstChoice human brain reference RNA Cat#AM6050, Life Technologies Inc., Carlsbad, CA) were included with each of the five datasets ...
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bioRxiv - Synthetic Biology 2021Quote: ... supplemented with anti-human CD3/CD28 magnetic Dynabeads (CTS, ThermoFisher) in a 1:1 ratio with cells ...
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bioRxiv - Cell Biology 2020Quote: Human fibroblast cells lines were maintained in DMEM/F12 (Gibco) with 15% FBS (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human fibroblasts were grown in MEM (Gibco, Life Technologies) supplemented with 20% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human fibroblasts were grown in MEM (Gibco, Life Technologies) supplemented with 20% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: ... Human IL-6 and IL-8 ELISAs were from Thermofisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... PE-conjugated mouse anti-human CD270 (ThermoFisher Scientific, MA, USA), and Pacific Blue-conjugated mouse anti-human CD274 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... FITC-conjugated mouse anti-human SOX2 (ThermoFisher Scientific, MA, USA), BV421-conjugated mouse anti-human CD34 (Biolegend ...
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bioRxiv - Immunology 2021Quote: Human embryonic kidney cells (HEK293) were maintained in IMDM (Gibco) supplemented with 10% FCS and 1% penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: Human PSCs resistant to neomycin were incubated with Accutase (Gibco) in 37°C for 5-10 minutes ...
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bioRxiv - Immunology 2021Quote: ... Human staining panels included anti-CD3-Alexa 700 (Invitrogen, UCHT1), anti-CD19-APC (Biolegend ...
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bioRxiv - Molecular Biology 2022Quote: Human Embryonic Kidney (HEK293) T-Rex Flp-In (ThermoFisher Scientific) cells were grown in DMEM supplemented with 10% FBS in an atmosphere of 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... anti-human IgG secondary antibody labeled with Dylight 650 (Invitrogen) was applied at 1 to 1000 (∼0.5μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... and 20 ng/mL human heat stable sbFGF (Gibco, PHG0367) and split using accutase (Millipore Sigma ...