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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... added with biotinylated anti-mouse C1q antibody (Fisher Scientific), and incubated for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... biotinylated capture antibodies (AT8: Invitrogen, REF MN1020B; HT7: Invitrogen, REF MN1000B ...
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bioRxiv - Immunology 2023Quote: ... and horse anti-mouse biotinylated (1:350 Life Technologies). Sections were then incubated in streptavidin-AF594 solution (1:750 μl ...
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bioRxiv - Molecular Biology 2023Quote: ... biotinylated RNA was enriched using M280 Streptavidin Dynabeads (Invitrogen) and precipitated using ethanol ...
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bioRxiv - Microbiology 2024Quote: Biotinylated DNA and Dynabeads MyOne Streptavidin C1 (Thermo Scientific) were mixed with PBB buffer (50 mM Tris/HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: Reactivity of recombinant S proteins from Wuhan and VOC Omicron as well as of recombinant RBD (Wuhan, Delta and Omicron) to large set (>9000) of human proteins was assessed by using protein microarrays (ProtoArray®, Invitrogen, Thermo Fischer). First microarray chips were blocked by incubation for 1 hour at 4 °C with buffer containing ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitation of Pol II-RNA complexes was performed using 50μl of Protein G Dynabeads (Thermo Fisher Scientific), pre-incubated over night with 5μg of the correspondent antibody ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% antibiotic-antimycotic and 1% protein-free hybridoma medium II (PFHM 2) (Thermo Fisher Scientific) and the respective growth factors and small molecules (8 µM GSK-3 inhibitor CHIR99021 (R&D Systems) ...
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bioRxiv - Microbiology 2023Quote: ... Biofilm cells were resuspended in 200 µl of B-PER II Bacterial Protein Extraction Reagent (Thermo Scientific) and incubated for 15 minutes at room temperature ...
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bioRxiv - Physiology 2023Quote: ... Proteins were then transferred from Bis-Tris gel onto PVDF membrane via XCell II blot module (Invitrogen) for immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... 25ng/mL recombinant murine EGF (Gibco), 50ng/mL recombinant human HGF (Peprotech) ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... and RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... we added recombinant mouse IFNγ (ThermoFisher), 2-deoxyglucose (Abcam) ...
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bioRxiv - Microbiology 2022Quote: ... and RNAseOUT recombinant ribonuclease inhibitor (Invitrogen). Next ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant IGF1 (Life Technologies, cat# PHG0071) and picropodophyllin (PPP ...
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bioRxiv - Genomics 2019Quote: ... 50ng/mL recombinant murine EGF (Gibco), and 100ug/mL recombinant human Noggin (Peprotech) ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant vitronectin (A14701SA, Thermo Fisher) was diluted in DPBS at the concentration of 1:200 to get the final concentration of 2.5 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant TNFα (Thermo Fisher, cat# PHC3015) was reconstituted at 0.1mg/ml in water ...
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bioRxiv - Cell Biology 2023Quote: ... mouse recombinant Egf (Life Technologies #PMG8043), 100 ng/mL mouse recombinant Noggin (Peprotech #250-38 ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant DKK1 was purchased from Gibco through Fisher (catalog number PHC9214) ...
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bioRxiv - Biochemistry 2019Quote: ... SAMS peptide substrate (His-Met-Arg-Ser-Ala-Met-Ser-Gly-Leu-His-Leu-Val-Lys-Arg-Arg, obtained from Invitrogen, USA) was used for the assay ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder, Applied Biosystems, in 10 μL of Hi-Di formamide, Applied Biosystems) and incubated at room temperature for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder [Applied Biosystems] in 10 μL of Hi-Di formamide [Applied Biosystems]) and incubated at room temperature for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Cry1 pcDNA4A-myc-his and Cry2 pcDNA4A-myc-his plasmids were double digested with NotI and XhoI FastDigest enzymes (Thermo Scientific) and used as host plasmids ...
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bioRxiv - Biochemistry 2023Quote: Plasmids of pET28a-his TEV-glnR or pET28a-his TEV-glnR derivative was firstly transformed into expression strain BL21(DE3) (Invitrogen, Inc.). then single colonies of the positive transformants were inoculated and amplified with 5 L LB broth supplemented with 50 μg/ml kanamycin at 37 °C with shaking ...
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bioRxiv - Cell Biology 2020Quote: ... fluorescently tagged BODIPY® (BODIPY® FL C12; Invitrogen D3822) stock was dried down completely in a speed vacuum and re-suspended in canola oil to a final concentration of 1mg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... and were incubated and tagged with CFSE (Invitrogen CellTrace, C34570) at 1 µL/mL for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... These LAP tagged contructs were co-tansfected with pOG44 (Invitrogen) into Flp-In HeLa T-REx cells ...
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bioRxiv - Developmental Biology 2019Quote: ... FLAG/HA-tagged AKAP2 was cloned using Gateway technology (Invitrogen) by PCR amplification of the AKAP2 cDNA using attB-flanked BP primers (see Table S2) ...
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bioRxiv - Molecular Biology 2020Quote: ... for YFP-tagged α globin and Neon electroporation system (Invitrogen, according to manufacturer’s instructions ...
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: Flag tagged Nef gene was constructed into pLenti vector (Invitrogen). pLenti-Nef plasmids or pLenti empty vectors were co-transfected with pLP1 ...
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bioRxiv - Physiology 2020Quote: ... appropriate species-specific fluorescently tagged secondary antibodies (Invitrogen, Carlsbad, CA) were used before mounting with DAPI (Vector Laboratories ...
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bioRxiv - Cell Biology 2022Quote: Myc-tagged p57 and p57CK- in pcDNA3.1+ Hygro vector (Invitrogen) were described previously [5] ...
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bioRxiv - Cell Biology 2024Quote: ... APEX2-tagged hiPSCs were washed with DPBS (Thermo Fisher, 14190136) and then incubated in DPBS containing 0.5 mM biotin-phenol (Iris Biotech ...
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bioRxiv - Neuroscience 2023Quote: ... and secondary AlexaFluor 488 tagged anti-Rabbit (Life Technologies; A21206). Sections were mounted using Prolong Gold Anti-Fade Reagent (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... incubated with appropriate fluorescent-tagged secondary antibodies (1:250, Invitrogen) for two hours at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... and a Cy5-tagged secondary antibody (1:2000, Thermo Fisher Scientific Inc. ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with alexa-fluor tagged secondary antibodies (Invitrogen) at RT for 2 hours and the nuclei were labelled using DAP (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Cell Biology 2023Quote: ... The bead-bound biotinylated proteins were analyzed by Western blotting using HRP-streptavidin diluted 1:40 000 in 3% BSA/TBST (Thermo Fisher Scientific, Rockford, IL, USA) and identified by mass spectrometry.
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bioRxiv - Bioengineering 2022Quote: ... Anti-human IL6 Ab concentrations were quantified using the Quant-it protein assay kit (Life Technologies). Ab conjugates were validated on 10% TBE urea denaturing gels and validated on an agarose gel (Supplementary Figure S8) ...
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bioRxiv - Immunology 2022Quote: ... The secreted fusion protein was purified on a CaptureSelect™ human albumin affinity matrix (Life Technologies), and protein eluted by adding 20 mM Tris and 2.0 M MgCl2 ...
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bioRxiv - Genomics 2022Quote: ... human preadipocytes in a 6-well plate were transfected with 6.25ug Cas9 protein (Fisher Scientific, A36498) and 800ng sgRNAs (IDT) ...
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bioRxiv - Microbiology 2023Quote: Human ACE2 fusion proteins were obtained from transient transfection using the Expi293TM system (Thermo Fisher Scientific). All plasmids used in this study were generated by ATUM Bio and transient transfection was carried out following the manufacturer’s protocol for the ExpiFectamineTM 293 transfection kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: ... and pol μ antibodies were affinity purified from rabbit serum by coupling each full-length recombinant protein to AminoLink Coupling Resin (Thermo Scientific) and following manufacturer’s instructions for IgG purification.
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bioRxiv - Microbiology 2019Quote: ... 125ng of total recombinant protein was separated on 4-20% or 10-20% Novex WedgeWell Tris-Glycine precast gels (Thermofisher, Waltham, MA). For silver stain ...
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bioRxiv - Cell Biology 2020Quote: ... specimens were incubated with 10 nM purified recombinant POLArISact protein or 2-5 nM Alexa Fluor 488 Phalloidin (AF488-phalloidin, A12379, Thermo Fisher Scientific) and 10 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Immunology 2020Quote: His-tagged recombinant mouse CD47 (as well as proteins and antibodies noted later) was labeled using AlexaFluor488 NHS Ester (Succinimidyl Ester, Thermo Fisher Scientific) reconstituted in anhydrous DMSO (dimethylsulfoxide ...
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bioRxiv - Bioengineering 2021Quote: ... S1-RBD and ACE2 recombinant proteins were expressed in Expi293 cells for 5 days following transient transfection using Expifectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell cultures were centrifuged at 4500g for 40 min ...