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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for Mouse Bcl 2 Modifying Factor BMF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA from mouse leukemia samples was isolated using the GeneJET Genomic DNA purification kit (Thermo Fisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Naive CD4+ T cells were isolated from the spleen and pLNs by mouse naive CD4+ enrichment kit (Invitrogen) per the manufacturer’s protocols ...
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: ... Flow cytometry fluorophore compensation for antibodies was performed using an AbC™ Anti-Mouse Bead Kit (ThermoFisher Scientific). Flow cytometry data were analyzed using FlowJo software (TreeStar ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM Tris(2-carboxyethyl)phosphine (TCEP, ThermoFisher)) supplemented with a protease inhibitor tablet (Roche ...
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bioRxiv - Genetics 2019Quote: ... 2 μl of probe (2 μM; Applied Biosystems) and 2 μl of template DNA (where extracts were normalised to 34 ng/μl) ...
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bioRxiv - Genomics 2019Quote: ... washed in 0.1% PBS/Tween and then incubated for 2 hours in secondary antibody (Goat anti-mouse 488 Life Technologies ab150113, Rabbit anti-goat 488 Life Technologies A27012). After washing ...
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bioRxiv - Microbiology 2019Quote: ... and 2 µL of the cDNA was analyzed by qPCR using a primer/probe set specific to mouse PKR sequence (Thermo Fisher, Mm01235643_m1, #4331182). The results were normalized to GAPDH ...
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bioRxiv - Cancer Biology 2019Quote: ... the sections were incubated overnight at room temperature with the indicated primary antibody prepared in blocking/permeabilization solution: mouse monoclonal antibody against HK-2 (1:100; Thermo Fisher, MA5-15679) or mouse monoclonal antibody against GLUT-3 (1:100 ...
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bioRxiv - Systems Biology 2020Quote: ... We cultured the mouse embryonic stem cell (ESC) line in 2-inhibitor based medium (Advanced DMEM/F12:Neurobasal (1:1) medium (Thermo Fisher, 12634028, 10888022) supplemented with 2.5% ESC-grade Tet-negative fetal bovine serum (vol/vol ...
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bioRxiv - Bioengineering 2022Quote: ... Sections were then incubated for 2 h at room temperature with an appropriate secondary antibody consisting of goat anti-mouse Alexa Fluor 488 (A11029, Fisher Scientific, Pittsburgh, USA) diluted 1:500 and wheat germ agglutinin Alexa Fluor™ 350 Conjugate (W11263 ...
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bioRxiv - Neuroscience 2020Quote: ... a secondary antibody was applied to the sections for 2 hours (AlexaFluor goat anti-mouse 594 diluted 1:400 in blocking buffer; Life Technologies, Carlsbad, CA). Sections were again washed in PBS over 30 minutes prior to the application of mounting media and coverslips.
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-mouse IgG1 Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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bioRxiv - Microbiology 2022Quote: ... Sections were washed 2 times with TBS + 0.025% triton then incubated with secondary antibodies donkey anti-mouse Alexa Fluor 647 (ThermoFisher, Waltham, MA, catalogue # A31571), donkey anti-rabbit Alexa Fluor 594 (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated for 1 h with an F(ab’)2-Goat anti-Mouse IgG (H+L) cross-adsorbed secondary antibody conjugated with Alexa Fluor 488 (Invitrogen, Thermo Fisher Scientific) diluted in blocking buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were rinsed (3 times) with 2 ml incubation buffer and resuspended with anti-mouse Alexa Fluor 647 secondary antibody (1:2000, Invitrogen, Carlsbad, CA, USA) for 60 minutes at RT ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at RT and then in the blocking buffer containing monoclonal anti-GFP mouse antibody (GF28R, 1:3000, Thermo Fisher, #MA5-15256) and biotin-conjugated anti-ALFA nanobody (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... Magnetic bead removal and the evaluation of CAR expression on T cells by flow cytometry were performed on day 6 by staining with a goat anti-mouse F(ab’)2 antibody (Invitrogen, Carlsbad, CA, USA). CART cells were harvested and cryopreserved on day 8 for future experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... which was detected by the secondary antibody against a mouse IgG (H+L) F(ab’)2 fragment conjugated to AlexaFluor 647 (Molecular Probes, Thermo Fisher Scientific).
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bioRxiv - Physiology 2023Quote: ... incubated at room temperature for one hour with Alexa Fluor 568-conjugated goat anti-mouse IgG (2 μg/ml, Molecular Probes, Cat #A11004) and Alexa Fluor 647-conjugated donkey anti-rabbit IgG (2 μg/ml ...
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bioRxiv - Physiology 2023Quote: ... incubated at room temperature for one hour with Alexa Fluor 647-conjugated donkey anti-mouse IgG diluted in blocking buffer (2 μg/ml, Molecular Probes, Cat #A31571) followed by washes in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were further incubated for 2 h at room temperature with HRP-tagged secondary antibody of goat anti-mouse (1:1000) (Life Technologies, Thermo Scientific, USA). The sections were then washed with TBST three times ...
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bioRxiv - Bioengineering 2023Quote: ... then incubated at room temperature in the dark for 2 h with secondary antibodies (AlexaFluor 488 goat anti-mouse IgG 1:400 or 1:600) or rhodamine phalloidin (Invitrogen, R415, 1:600) to visualize F-actin ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues from L1/2-L6/S1 and Ca1/2-Ca14/15 of same mouse were pooled and served as a single sample stored into RNAlater® Reagent (Invitrogen, Carlsbad, CA) for minimum 2 days at -80°C (n = 7 mice/genotype ...
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bioRxiv - Immunology 2023Quote: ... Mouse T cells were maintained in RPMI-1640 supplemented with sodium pyruvate and 2-mercaptoethanol (both from Life Technologies Corporation, Carlsbad, CA, USA). All media was supplemented with 10 % fetal bovine serum ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were plated in a round bottom 96-well plate at 2 × 105 cells/well and activated using Mouse T Activator CD3/CD28 Dynabeads™ (Gibco; Cat # 11456D). 11-Cl-BBQ was added at a concentration of 100nM ...
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bioRxiv - Neuroscience 2023Quote: ... magnetic beads (30 µL) were incubated at room temperature for 2 hours with 1 µg mouse monoclonal anti-GFP (Invitrogen 3E6 A-11120), washed thrice with Wash Buffer (150 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 % SDS) for 30 minutes at 50 °C prior to reprobing using mouse anti-3-phosphoglycerate kinase anti-serum (Invitrogen-Molecular probes, A6457).
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bioRxiv - Molecular Biology 2020Quote: ... 2 (Thermofisher). A reverse transfection approach was used to deplete AurkA and CEP135 ...
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bioRxiv - Systems Biology 2023Quote: ... 2% (ThermoFisher). Purified amplicons were quantified by Qubit™ dsDNA Quantification Assay Kits (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... were co-transfected with the EGFP-Caspase 2 or EGFP-Δ Caspase 2 plasmid and the synthesized miR-2 (miR-2-mimic) using Cellfectin II transfection reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Macrophages were incubated with the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (10 μM, Invitrogen, California, USA) in PBS for 30 min ...
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bioRxiv - Immunology 2021Quote: ... containing 2’-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 50 μM; Thermo Fisher Scientific). To determine FA uptake ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Immunology 2023Quote: ... stained with Ghost-510 and 2-(N-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 60 µM) (Thermo Fisher Scientific) (30 min at 37 C) ...
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bioRxiv - Pathology 2024Quote: ... HT7 was the only anti-mouse antibody used and a mouse-on-mouse blocking agent (Invitrogen: R37621) was applied for 30 minutes post protein block washes to reduce noise from secondary antibody binding and an additional three 5-minute washes were added before primary antibody incubation ...
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bioRxiv - Neuroscience 2023Quote: ... Sections stained with mouse primary antibodies were blocked in ReadyProbes “Mouse on Mouse” IgG blocking solution (Thermofisher) for 1 hour before the initial blocking step ...
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bioRxiv - Cell Biology 2020Quote: ... and sorted into Ham’s F-10 plating medium (Cellgro) supplemented with 5 ng/mL basic fibroblast growth factor (Invitrogen), 10% FBS (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... Transferrin (Tf) and Epidermal Growth Factor (EGF) tagged with Alexa Fluor 488 was obtained from Invitrogen (T13342 and E13345). Dako fluorescent mounting medium from Agilent (S3023 ...
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bioRxiv - Immunology 2022Quote: ... ICFC was performed with the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, Catalog # 00-5523-00). Cells were surface stained as described above ...
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bioRxiv - Immunology 2021Quote: ... Samples were stained with 100μl of intracellular anti-transcription factor stain: eFluor 450-conjugated anti-FOXP3 (FJK-16s, ThermoFisher), PE-Cy7-conjugated anti-T-bet (eBio4B10 ...
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant Epidermal Growth Factor 1-53 (EGF 1-53, 5 ng/ml, Thermo Fisher Scientific, catalog #17005-042) and 1% penicillin/streptomycin ...
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bioRxiv - Genetics 2019Quote: ... were cultured with mouse embryonic fibroblasts in the presence of leukemia inhibitory factor (LIF; 1000 U/ml) in Glasgow modification of Eagle’s medium (GMEM; Invitrogen) containing 10% fetal calf serum ...
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bioRxiv - Bioengineering 2020Quote: ... PSC lines were maintained in undifferentiated state in feeder-free conditions on growth factor-reduced Geltrex (Thermo Fisher Scientific) in E8 medium (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were purchased from Kerafast and grown in keratinocyte serum-free media supplemented with bovine pituitary extract and recombinant epidermal growth factor (Gibco, #37010022) with 1% Penicillin-Streptomycin ...
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bioRxiv - Cancer Biology 2020Quote: ... was co-transfected with 300 ng of plasmid expressing test transcription factor constructs (EmptyVector, pmax-C11orf95fus1, pmax-C11orf95-RELAfus1, pmax-RELA, pmax-C11orf95fus1-VP64) using 1.5 µL Lipofectamine 3000 (ThermoFisher). 48 hours after transfection ...
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bioRxiv - Microbiology 2022Quote: ... and fixed for 30 minutes with fixation/permeabilisation buffer of eBioscience FOXP3/Transcription Factor Staining Buffer Set (ThermoFisher Scientific). The cells were then washed with permeabilisation buffer ...