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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for Mouse Anti Human Immunodeficiency Virus HIV 1 p17 1981 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Blots were washed 3x with PBS-T and incubated for 1 h at room temperature with appropriate secondary antibodies: mouse IgGk-BP-HRP (cat# sc-516102, SantaCruz) or goat anti-human-HRP (cat# 31410, Thermo Scientific), diluted 1:10,000 in 5% blocking buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... the sections were treated with goat-anti-mouse IgG Alexa Fluor 488 and goat-anti-mouse IgG Alexa Fluor 555 for 1 hour (both from Thermo Fisher Scientific, MA, USA). The staining procedure was conducted using the Antifade Reagent with DAPI (Cell Signaling Technology ...
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bioRxiv - Immunology 2023Quote: ... A secondary antibody was used in TBS-T with a dilution of 1:5,000 for Anti-Mouse (Thermo Fisher, Goat anti-Mouse IgG, #31430) and 1:80,000 for Anti-Rabbit antibody (Merck Anti-Rabbit IgG ...
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bioRxiv - Neuroscience 2023Quote: ... Iowa City, IA), mouse anti-Futsch (22C10 DSHB, Iowa City, IA) and mouse anti-GFP at 1:1000 (#A-11120, Thermo Fisher Scientific, Waltham, MA). Secondary antibodies used in this study were goat anti-mouse Alexa Fluor 488-conjugated IgG at 1:500 (#A-32723 ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections 50 um thick were immunostained for tyrosine hydroxylase (primary: mouse anti-TH, Millipore #MAB318; secondary: goat anti-mouse 647, Invitrogen #A-21236 or goat anti-mouse 405, Invitrogen #A-31553), tryptophan hydroxylase 2 (primary ...
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bioRxiv - Microbiology 2021Quote: ... or Dynabeads human T-activator anti-TCR/anti-CD28 (Gibco, 11132) (1 bead/cell ...
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bioRxiv - Microbiology 2021Quote: ... and Dynabeads human T-activator anti-TCR/anti-CD28 (Gibco, 11132) (1 bead/cell) ...
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bioRxiv - Microbiology 2022Quote: ... Goat anti-mouse IgG Alexa Fluor 488 (1:500 dilution in 1% BSA in PBS; Invitrogen) was used as the secondary antibody ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 1 h with secondary antibodies (anti-Mouse IgG 488, 1:1000 Invitrogen, #A11029 ...
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bioRxiv - Microbiology 2020Quote: ... followed by staining for 1 h with anti-mouse AF647 (1:1000; A-28181, Molecular Probes). The membrane was washed 3 times for 5 minutes with PBS containing 0.05% Tween 20 with rocking between each step ...
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bioRxiv - Developmental Biology 2019Quote: ... slides were incubated 1 hr with secondary antibody (Alexa 647 donkey anti-mouse, Invitrogen, 1:500) in the above buffer ...
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bioRxiv - Microbiology 2021Quote: ... followed by 1 hour incubation at room temperature with goat anti-mouse AF488 (ThermoFisher, 1:400), goat anti-rabbit AF647 (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were then stained overnight with primary antibody mouse anti-ZO-1 (1:250; Invitrogen #339100) in blocking buffer at 4°C ...
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bioRxiv - Genetics 2024Quote: ... The following antibodies were used: mouse anti-ZO-1 (1:500, Thermofisher Cat. No. 33-9100), rabbit anti-Glutamine synthetase (GS ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were immunostained according to standard protocols [21] using a primary rabbit anti-human antibody directed against zona occludens-1 (ZO-1; 1:100, Thermo-Fisher Scientific, UK) and a AF488-conjugated secondary goat anti-rabbit antibody (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... goat anti-mouse Alexa-Fluor 488, 1:500, Thermofisher Scientific; Iba1: goat anti-chicken Alexa-Fluor 568, 1:500, Thermofisher Scientific) for 4 hours at RT ...
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bioRxiv - Cell Biology 2019Quote: ... The following secondary antibodies were used: goat anti-mouse IgG2b Alexa 568 (1:500) and goat anti-guinea pig Alexa 488 (1:1000) (Molecular Probes, Invitrogen), donkey anti-guinea pig Cy3 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Donkey anti-rabbit HRP-conjugated (Cytiva, NA9340V, 1 to 5000) and goat anti-mouse HRP-conjugated (Invitrogen, 32430, 1 to 10000) were used for 1h incubation at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... secondary antibodies were added and incubated for 45 min at RT: anti-mouse Alexa488-conjugated (1:500) and anti-rabbit Cy3-conjugated (1:500) (Molecular Probes). After washing (3x in 1x PBS ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit polyclonal anti-CLC7 (1:4000; AP11863b, Abgent, San Diego, CA) or mouse monoclonal anti-GAPDH (1:10,000; AM4300, Thermo Fisher Scientific) and horseradish peroxidase-conjugated goat anti-mouse or anti-rabbit secondary antibodies (1 ...
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bioRxiv - Neuroscience 2022Quote: ... goat anti-mouse Alexa-Fluor 488, 1:500, Thermofisher Scientific, dsRed: goat anti-rabbit Alexa-Flour 568, 1:500, Thermofisher Scientific) for 4 hours at RT ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibody (D1R: anti-D1R [mouse] 1:1500, Novus Biologicals, 48 hrs at 4°C; Th: anti-Th [rabbit] 1:1000, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... followed by co-incubation with goat anti-guinea pig IgG (1:1000) and anti-mouse IgG (1:1000) conjugated to Alexa 488 and Alexa 594 (Molecular Probes), respectively ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then probed with anti-p75NTR (1:200; rabbit monoclonal, kindly gifted by P. Barker) and anti-GAPDH 1:8000 (mouse monoclonal IgG; Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... a rabbit anti-GFP antibody (1:2000, Yeasen Biotechnology Co., Ltd) and a Mouse anti-β-actin antibody (1:2000, Invitrogen) were added and incubated at 4°C overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... Mouse anti-arm antibody at 1:1500 dilution or rabbit anti-FLAG antibody at 1:1000 dilution and goat anti-rabbit IgG–AP conjugate at 1:2000 or goat anti-mouse IgG-AP conjugate at 1:200 dilution (Molecular Probes) was used as primary and secondary antibodies to probe the blot ...
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bioRxiv - Cell Biology 2023Quote: ... horseradish peroxidase (HRP)-conjugated anti-mouse IgG (NA931, 1:10000), and anti-rabbit IgG (NA934, 1:10000) were purchased from Thermo Fisher Scientific.
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 120min at room temperature with biotinylated horse anti-mouse (1:200, ref A-2001, Vector) and goat anti-chicken Alexa 633 (1:250, ref A21103, Molecular Probes) secondary antibodies in a solution containing horse serum (5% ...
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bioRxiv - Biophysics 2023Quote: ... blocked and imaged as described under section triple-color immunostainings but were incubated with mouse anti-AnkG (#AB_10675130, Neuromab, 1:200) overnight at 4°C and subsequently with donkey anti-mouse AF647 (#A31571, Invitrogen, 1:800) for 1h.
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bioRxiv - Cancer Biology 2023Quote: ... antibody overnight at 4 °C and then with secondary antibody (1:1000 goat anti-mouse Alexa Fluor 488 or 1:2000 goat anti rabbit Alexa Fluor 647) and DAPI (Thermo Fisher). Image acquisition was performed with a confocal microscope (LEICA SP8 Confocal Microscope).
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bioRxiv - Cell Biology 2022Quote: ... antibodies were used followed by specific secondary Alexa Fluor antibodies (1:500 anti-mouse Alexa Fluor 546 and 1:500 anti-rabbit Alexa 488 Thermo Fisher). For actin staining ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies included horseradish-peroxidase-conjugated anti-mouse or anti-rabbit IgG (1:2000 or 1:5000; ThermoFisher, 31430 or 31460). Proteins were detected by ECL chemiluminescence detection kits (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... the sections were washed three times with PBS 1X and then incubated for 1 hour with donkey anti-mouse (or anti-rabbit) Alexa Fluor 488nm (or 555nm) secondary antibodies (1:200, Thermofisher Scientific). DAPI (Roche ...
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bioRxiv - Microbiology 2019Quote: ... The viral N-protein was labeled using a primary anti-Tula virus antibody(12) and a secondary anti-rabbit FITC conjugate (Invitrogen, USA). The viral Gc protein was stained using a mouse monoclonal antibody (#H1808-60B ...
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bioRxiv - Immunology 2021Quote: ... Virus was titrated by adding serial dilutions of virus supernatant (8 replicates) on VeroE6 cells in DMEM (Gibco) containing 2% FBS ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with ketamine-xylazine and infected intranasally with 5,000 PFU of the non-recombinant virus or 1,000 PFU of the recombinant virus in 50 μl of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). For antibody treatment ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with appropriate secondary antibodies (488 anti-chicken, 546 anti-mouse, 647 anti-guinea pig, 647 anti-rabbit, 647 anti-mouse, Alexa Fluor, Life Technologies)
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bioRxiv - Microbiology 2020Quote: ... qPCR was performed using the TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems). Assays were conducted on a QuantStudio5 Q-PCR machine (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... The assay was performed using the TaqMan® Fast Virus 1-Step Kit (Life Technologies) and kit manual ...
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bioRxiv - Neuroscience 2021Quote: ... using TaqMan Fast Virus 1-Step Master Mix (PN4453800, Applied biosystems, Thermo Fisher Scientific, USA). PrimerTime primers and FAM-dye coupled detection probes were used for detecting Clstn3 mRNA level ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL of purified virus was analyzed with the Quant-it PicoGreen dsDNA Assay (Invitrogen).
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bioRxiv - Immunology 2022Quote: ... For these assays we used TaqMan Fast Virus 1- Step Master Mix (ThermoFisher, catalog # 4444432) and custom primers/probes targeting the E gene sgRNA (forward primer ...
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bioRxiv - Neuroscience 2021Quote: ... using TaqMan Fast Virus 1-Step Master Mix (PN4453800, Applied biosystems, Thermo Fisher Scientific, USA). PrimerTime primers and FAM-dye coupled detection probes were used for detecting Clstn3 mRNA level ...
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bioRxiv - Microbiology 2019Quote: Concentrated virus aliquots were mixed with DiI (final concentration of 1 μM, Thermo Fisher, USA), vortexed and incubated for 1.5 h at room temperature (RT) ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR reactions were performed using TaqMan® Fast Virus 1-Step Master Mix (ThermoFisher) and 5 µl of isolated RNA as a template to detect a 132 bp sequence in the ORF1b/NSP14 ...
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bioRxiv - Microbiology 2022Quote: ... qPCRs were set up using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher) and run on an ABI 7500 Fast Real-time PCR System (Applied Biosystems/Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR was performed with TaqMan Fast Virus 1-Step Master Mix (Invitrogen, cat. 4444434) in QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... vRNA was reverse transcribed using the TaqMan Fast Virus 1-Step qRT-PCR kit (Invitrogen) and quantified on a LightCycler 480 instrument (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were washed three times again in PBS with 0.2% Triton X-100 and transferred into secondary antibody solution (anti-chicken Alexa Fluor 488, 1:200; anti-mouse Alexa Fluor 647, 1:200; anti-rabbit Alexa Fluor 647, 1:200; Molecular Probes, Eugene, OR, USA) and incubated for 48 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... 1:200) at 4 °C 48 hours and in secondary antibodies simultaneously (Goat Anti-mouse 1:200, Invitrogen, Goat Anti-Rabbit 1:200, Jackson) at RT for 1 hour ...