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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for 6 Chloro 2 3 4 9 tetrahydro 1H pyrido 3 4 b indol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... Gdf1/3 and Gdf1/3-like mRNA were synthesized using T7 mMESSAGE mMACHINE kit (Ambion). Unfertilized eggs were injected with mRNAs and fertilized as previously described50 ...
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bioRxiv - Biophysics 2023Quote: ... 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine (DiI) and 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindodicarbocyanine (DiD)) were purchased from ThermoFisher Scientific and used without additional purification ...
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bioRxiv - Cell Biology 2021Quote: ... mouse HMB45 (1:20; 4°C overnight; Life Technologies, 081050). Afterwards ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg/mL of FM 4-64 Dye (ThermoFisher, T13320) was contained in agarose media ...
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bioRxiv - Biophysics 2022Quote: ... and 4 µM Oregon Green 488 BAPTA-1 (Fisher Scientific) in extracellular buffer for 30 minutes at 37 °C ...
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bioRxiv - Systems Biology 2022Quote: ... mixed in a 4:1 ratio with M199 (Thermofisher, 41150020), 10% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... containing 4% FCS and 1% L-Glutamine (L-Gln; Invitrogen). These adherent cells were cultured to confluence for five days at 37 °C in an atmosphere containing 5% CO2.
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bioRxiv - Neuroscience 2024Quote: ... and rabbit anti-synaptophysin Ab-4 (Fisher Scientific, 1:100). Samples were washed extensively with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... the fluorescent Ca2+ indicator Fluo-4-AM (1:500; Invitrogen), and 20% pluronic acid F-127 (1:500 ...
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bioRxiv - Biophysics 2023Quote: ... Sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Thermo Fisher, A39268) (Sulfo-SMCC ...
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bioRxiv - Neuroscience 2023Quote: ... and processed at 4°C with NeuroTrace (1:500, Invitrogen) in PBS containing 0.3% Triton-X ...
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bioRxiv - Molecular Biology 2024Quote: ... oligodendrocytes were loaded with 1 μM Fluo-4 AM (Invitrogen) in culture medium for 30 min at 37°C and then washed ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin was stained with To-Pro-3 (1/200 dilution, 1 mM, Invitrogen T3605).
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bioRxiv - Neuroscience 2022Quote: ... dried and coverslipped under glycerol:TBS (3:1) with Hoechst 33342 (1:1000; ThermoFisher Scientific). Primary antibodies with the following dilutions used in this study include ...
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bioRxiv - Pathology 2024Quote: ... and 3-hydroxybutyrate dehydrogenase 1 (15417-1-AP, Thermo Fisher Sci, Waltham, MA, USA). HRP-conjugated secondary antibodies (NA9340 ...
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bioRxiv - Biochemistry 2024Quote: ... columns were washed with 3 column volumes (CV) of 1:1 RNase ZAP (Ambion) followed by 3 CV nuclease-free water (DEPC-treated MilliQ water) ...
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bioRxiv - Microbiology 2021Quote: ... lysates were spun at 10,000 x g for 10 minutes at 4 °C prior to reaction with 4- acetamido-4’-maleimidyl-stilbene-2,2’-disulfonic acid (AMS) (ThermoFisher Scientific). AMS alkylation was performed by vortexing the lysates in 15 mM AMS ...
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bioRxiv - Cell Biology 2019Quote: The mRFP-CLASP2γ U2OS cells expressing H2B-GFP were cultured in glass coverslips using DMEM without phenol red and supplemented with 25 mM of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Gibco) and 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... then the lysate equivalent to 2×105 cells was mixed at a 1:4 ratio with 4x LDS buffer containing 10% sample reducing agent (Invitrogen). Samples were heated at 95°C for 5 minutes and collected by centrifugation before loading.
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bioRxiv - Bioengineering 2021Quote: ... the cells were incubated with 2 μM EthD-1 and 4 μM Calcein AM solutions of cell viability/cytotoxicity kit (Invitrogen) for 20 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen) in solution with PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... organoids were incubated with 2 mM Calcein-AM and 4 mM Ethidium homodimer-1 (LIVE/DEAD assay kit, Molecular Probes) for up to 6h ...
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bioRxiv - Neuroscience 2021Quote: ... Primary microglial cells were prepared from post-natal day 2-4 pups as previously described and cultured in DMEM containing 1% PSF (ThermoFisher) 10% heat inactivated FBS (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... N2a cells were transfected with pSpCas9(BB)-Puro empty vector or vectors expressing each of the three gRNAs targeting the eIF4E3 exons (meIF4E3 gRNA exon 1, exon 2, exon 4) using Lipofectamine 2000 (Invitrogen). pSpCas9(BB)-Puro was a generous gift from Dr ...
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bioRxiv - Cell Biology 2022Quote: ... Skeletal muscle were stained with Myosin 4 monoclonal antibody (MF20, MyHC-2) (1:200; Thermo Fisher Scientific, Rockford, IL, USA) and Laminin gamma 1 Antibody (A5 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were centrifuged at 500 ×g for 5 minutes at 4°C and washed and resuspended with ice-cold FACS Buffer (2 or 10% FBS in 1× HBSS (Gibco) or 1X PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... pMDG2.1-VSV-G and p8.91-GAG-POL in a ratio of 4:1:2 to a total of 10 μg of DNA using Lipofectamine 2000 (ThermoFisher) as per the manufacturer’s instructions overnight ...
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bioRxiv - Biochemistry 2022Quote: ... The filtrate was then incubated for 1 h rotating at 4 °C with 2 mL Ni-NTA resin (Thermo Fisher) per 1.5 L of expression culture ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were washed with assay buffer (PBS containing 0.2% BSA and 1 mM CaCl2) and resuspended in assay buffer with 2 μM Fluo-4-AM (Life Technologies). Fluo-4-AM was prepared as a 1 mM stock in DMSO ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated o/n at with rotation at 4°C with 2 µg IP antibody and for 1 h with Dynabeads Protein G (Invitrogen). Alternatively ...
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bioRxiv - Developmental Biology 2024Quote: ... for 2 hrs at RT and incubated overnight at 4°C in primary antibody solution (1:100 rabbit anti-GFP (ThermoFisher) in blocking reagent) ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were then washed in PBST-2 (PBS containing 0.1% Triton X-100) for 3 x 2 mins and blocked with SeaBlock blocking buffer (Thermo Fisher) for 15 mins at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... particle size = 3 μm, C18, L = 2 cm; analytical column: particle size = 2 μm, C18, L = 50 cm; PepMap, Dionex/Thermofisher). Peptides eluting from the column were ionized using an Orbitrap Elite (Thermofisher).
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bioRxiv - Microbiology 2021Quote: ... The LB medium contained 4 μg/mL FM 4-64 stain (ThermoFisher) to stain all cells ...
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bioRxiv - Biophysics 2022Quote: ... sorbitol (4% w/v) and glycerol (4% v/v) (Fisher Scientific, Mumbai). Growth rates were measured by monitoring optical density at 600 nm (OD600 ...
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bioRxiv - Biochemistry 2021Quote: ... Stained ovaries were finally mounted with one drop of vectashield in epoxy diagnostic slides (Thermo-Fisher Scientific, 3 wells 14 mm) and covered with high precision cover glasses ...
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bioRxiv - Microbiology 2024Quote: ... isolated RNA was run using a TaqMan Fast Virus One-Step Master Mix (applied Biosystems) on a QuantStudio 3 Flex Real-Time PCR system (Applied Biosystems). RNA standards with known copy numbers were used to generate a standard curve and calculate sample copy numbers ...
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bioRxiv - Bioengineering 2022Quote: ... Devices were washed with PBS for 4-6 hrs and subsequently incubated with Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) (ThermoFisher cat# A32728, 1:300) and Hoechst 33342 (Sigma-Aldrich cat# B2261 ...
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bioRxiv - Microbiology 2019Quote: ... The cells were plated on 6-well plates at 1 × 105 cells/well or onto 4-channel chamber slides (Lab-Tek II chamber slide, Thermo Fisher Scientific, Waltham, MA) at 5 × 104 cells/well and were grown until reaching 70% confluence in serum-free EpiLife medium supplemented with HKGS.
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bioRxiv - Cell Biology 2021Quote: ... Cells were pelleted at 3000 × g at 4°C for 30 mins and subsequently lysed with B-PER (Thermo Scientific) supplemented with 50 U DNAseI ...
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bioRxiv - Genomics 2019Quote: ... Protein amount was quantified by BCA protein assay reagents (Pierce) and normalized for loading on a 4–12% B-T NuPage gels (Invitrogen). After transfer on nitrocellulose membrane ...
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bioRxiv - Genetics 2021Quote: Total RNA was isolated from the eggs at 24 hpo from a wild-type strain (p50T) and three b-4 mutant strains (e36, e37, and No. 903) using TRIzol (Invitrogen). Reverse transcription was then performed using the oligo (dT ...
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bioRxiv - Immunology 2022Quote: MACS enriched naïve mouse B cells or Cas9 RNP transfected B cells were cultured for 4 days in 200 μL complete RPMI media per well of 96-well plate (Nunc) in the presence of 3 μg/ml anti-CD40 antibody (Biolegend ...
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bioRxiv - Biochemistry 2022Quote: ... Pellets were frozen for an hour at −20°C and resuspended in 4 mL of B-PER Reagent (Thermo Scientific) per gram of cell pellet with 50 mg/mL lysozyme and 250U/uL Benzoase (Sigma Aldrich NV) ...
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bioRxiv - Immunology 2024Quote: ... were irradiated with 80 Gy and co-cultured with primary splenic B cells for 4 days in high glucose DMEM medium (Gibco) supplemented with 10% FBS ...
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bioRxiv - Genetics 2023Quote: ... The eluents were ultrapure water (solvent A) and acetonitrile (ACN, LC-MS Grade, Thermo Scientific, Catalog #A955-4, solvent B), each containing 0.06% Trifluoroacetic Acid (TFA ...
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bioRxiv - Cell Biology 2023Quote: ... 5 ng/mL rh FGF-b, 25 µg/mL rh insulin, 4% FBS (PCS-950-040, ATCC) and penicillin-streptomycin (Gibco). 24-96 hrs after passaging ...
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bioRxiv - Cell Biology 2021Quote: ... Daily media changes were performed up to day 9 when cells were fixed with 100% ice-cold Methanol (Fisher Scientific, cat # A454-4). Images were acquired on a Nikon Instruments Ti2 inverted fluorescence microscope ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA was extracted from stages 2-4 hr embryos using Trizol reagent (Invitrogen). RNA quality of three biological replicates was tested by Agilent Bioanalyzer ...
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bioRxiv - Immunology 2022Quote: Overnight cultured-T cells were stained with 2 μM fluo-4 AM (Thermo Fisher) in Hank’s solution (SL6080 ...