Labshake search
Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2020Quote: ... EVs were depleted by centrifuging FBS at 100,000g for 1 hour at 4°C (Sorvall WX Ultra 80, Thermo Fisher Scientific, Waltham, MA) and using the supernatant (30) ...
-
bioRxiv - Biochemistry 2020Quote: ... Samples were run on 6% TBE gel in 1 x TBE (150 V, 90 min, 4°C) stained with SYBR Gold (Thermo Fisher Scientific. S11494) and InstantBlue Protein Stain (Expedeon).
-
bioRxiv - Neuroscience 2020Quote: ... The cells were incubated with Alexa 488-labeled H06 at 1 μg/mL or Alexa 488-labeled transferrin at 4 μg/mL (Thermofisher Scientific, Waltham, MA) for 16 hours at 37° C ...
-
bioRxiv - Bioengineering 2021Quote: ... Hydrogels were washed 4 × 20 minutes with PBST and then cultured in secondary antibody (Alexafluor 488 goat anti-rabbit; 1:500; Thermo Fisher A-11008) overnight at 4°C ...
-
bioRxiv - Systems Biology 2021Quote: ... Then embryos were detected by goat anti-rabbit cross-adsorbed Alexa Fluor 647-conjugated antibody at 1:500 dilution with DAPI overnight at 4°C (Thermo Fisher Scientific, #A21244).
-
bioRxiv - Neuroscience 2020Quote: ... This step was followed by 4 hour incubation at room temperature in Alexa Fluor goat anti-mouse 647 (1:300 µl; A-21235, Invitrogen, Grand Island, NY) and donkey anti-rabbit 555 (1:300 µl ...
-
bioRxiv - Neuroscience 2020Quote: ... and then incubated at room temperature for 4-5 h in Triton PBS containing Alexa Fluor 594-conjugated donkey anti-rabbit IgG (1:1000; A-21207, Thermo Fisher Scientific, RRID:AB_141637). After binding of streptavidin/antibodies ...
-
bioRxiv - Developmental Biology 2021Quote: ... Cells re-suspended at a concentration of 1000 cells/µl were incubated for 30 minutes at 4°C with 1 µl Red LIVE/DEAD Fixable Dead Cell Stain (Thermo Fisher Scientific – L23102). After one wash in PBS ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were again centrifuged at 250 rcf for 5 minutes at 4°C and subsequently incubated with matching secondary antibodies (1:200, donkey anti-sheep Alexa Fluor™ 647, ThermoFisher, #A-21448) for 30 minutes at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight at 4 °C with PE-conjugated LAMP1 CD107a clone H4A3 antibody (Cat no. 527993; Molecular Probes; 1:20). After 3× washes ...
-
bioRxiv - Cancer Biology 2021Quote: ... sections were incubated overnight at 4 °C with ~100ul primary antibodies diluted in 1% BSA+Triton-X (Thermo Fisher Scientific, Rockford, IL, USA) [1] ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells were then incubated overnight with primary antibodies at 4°C in B/P buffer containing either 1:50 rabbit anti-fatty acid synthetase (MA5-14887; Invitrogen, Waltham, MA, USA) or 1:500 rabbit anti-peroxisome proliferator-activated receptor gamma (ab45036 ...
-
bioRxiv - Synthetic Biology 2022Quote: The establishment of periphyton in our study was performed under static conditions in sterile 1-well Nunc™ Lab-Tek™ II Chamber Slides (74 × 25 mm, Surface = 1850 mm2, Volume = 4 mL, Thermo Scientific), which consists of a removable polystyrene media chamber attached to a standard glass slide ...
-
bioRxiv - Genomics 2022Quote: ... Plasmids were mixed in a 3:1 weight ratio of total plasmid DNA:PEI in 4 mL of either OptiMeM™ (Gibco cat. # 31985-062) or serum-free DMEM per flask and then shaken vigorously for 30 sec ...
-
bioRxiv - Biochemistry 2022Quote: Western blot: Pancreatic and islet lysates were run on a 1 mm 4-12% Bis-Tris gel (Novex Life Technologies; Thermo Fisher; #NP0321) with MES SDS running buffer (Invitrogen ...
-
bioRxiv - Biochemistry 2022Quote: Western blot: Pancreatic and islet lysates were run on a 1 mm 4-12% Bis-Tris gel (Novex Life Technologies; Thermo Fisher; #NP0321) with MES SDS running buffer (Invitrogen ...
-
bioRxiv - Microbiology 2022Quote: ... The in vitro transcription reaction for the 5’ cap driven Fluc reporter included a 1:4 ratio of Invitrogen™ Anti-Reverse Cap Analog (Fisher Scientific AM8045) and GTP solution (0.4 µl cap analog to 1.6 µl of GTP solution) ...
-
bioRxiv - Neuroscience 2021Quote: ... sections were incubated for two days at 4°C with the primary antibodies: 1:500 rabbit anti–PSD-95 (Invitrogen catalog#51-6900); 1:2500 guinea pig anti-VGlut1 (EMD Millipore catalog#AB230175) ...
-
bioRxiv - Neuroscience 2021Quote: ... preparations were washed six times with PBT and incubated for one night at 4°C with secondary antibodies goat anti-rabbit Alexa 488 (Invitrogen, USA; 1:250) and goat anti-mouse DyLight 649 (Jackson ImmunoResearch ...
-
bioRxiv - Immunology 2020Quote: ... the cells were washed by centrifugation (1.300 rpm, 4°C) and 50 μL of 1-step-Fix/Lyse solution (Thermo Fisher #00-5333-54) was added for 15 min at room temperature ...
-
bioRxiv - Molecular Biology 2020Quote: ... Primary antibody incubation was carried out at 4°C overnight with a rabbit polyclonal antibody to Creb5 (PA5-65593, Thermo Fisher, 1:100), a mouse monoclonal antibody to Prg4 (MABT401 ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were infected in a BSL-3 lab with the UF-1 strain of SARS-CoV-2 at MOI of 4 in media containing 3% low IgG FBS (Fisher Scientific, Cat. SH30070.03).
-
bioRxiv - Biochemistry 2021Quote: ... overnight at 4°C and then with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Thermo Fisher Scientific, A32723, dilution 1:1000) for 1 h in PBS containing 5% goat serum 25°C.
-
bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-mouse IgG1 Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
-
bioRxiv - Neuroscience 2022Quote: ... brains were incubated for three days at 4°C with either the Alexa Fluor 488 secondary antibody –conjugated goat anti-mouse (Fisher Scientific, 1:250) for subsequent two-photon imaging ...
-
bioRxiv - Developmental Biology 2022Quote: ... The samples were washed (4 times 30 min, PBST) and then incubated with the secondary antibody (Alexa fluor 647, 1: 1200 ThermoFisher in blocking solution) overnight at 4°C with gentle rotation ...
-
bioRxiv - Microbiology 2022Quote: ... pH 7.4 were incubated for 30 min at 37°C with 1 µg/mL trypsin (Pierce trypsin, TPCK treated from Thermo scientific cat. PI20233). Trypsin activity was terminated with 10 µM Aprotinin (bovine lung ...
-
bioRxiv - Microbiology 2022Quote: ... For agar experiments we additionally stained the cell body with 10 µl FM 4–64 (Thermo Fisher Scientific; 1 µg/µl in DMSO). It was added before the last washing step ...
-
bioRxiv - Plant Biology 2020Quote: ... and imaged using a 1:4 ratio of Supersignal® West Femto Maximum Sensitivity Substrates and Supersignal® West Pico Plus (Thermo Scientific) and a ChemiDoc Imaging System (Biorad) ...
-
bioRxiv - Neuroscience 2021Quote: ... Slices were then incubated overnight (4°C) in the same blocking solution containing the primary rabbit anti-PV antibody (1:1000; Thermo Scientific, PA1-933) and mouse anti-GFP antibody (1:500 ...
-
bioRxiv - Bioengineering 2020Quote: ... Scaffolds were washed in PBS at every timepoint (day 1, 4, 7, 14, 28) and placed in a solution of alamarBlue® (Invitrogen, California, USA) in an incubator at 37°C on a shaker ...
-
bioRxiv - Neuroscience 2020Quote: ... The cells were then incubated overnight at 4 °C in 1:100 (in antibody diluent) anti-cadherin-11 antibody (CDH-11, rabbit polyclonal, Thermo Fisher, 71-7600). Cells were washed three times with PBS-tween and incubated in the conjugated secondary antibody ...
-
bioRxiv - Neuroscience 2021Quote: ... The tissues were incubated overnight at 4 °C with the primary antibody (Rabbit anti-GFP; 1:500, Cat. A-11122, ThermoFisher Scientific, Waltham, MA) that was made in 0.5% PBST with 1% BSA ...
-
bioRxiv - Developmental Biology 2021Quote: ... overnight at 4 °C and then with the goat anti-rabbit Alexa Fluor 488 secondary antibody (Life Technologies, Ltd., Paisley, UK; 1:500) for 2 h at RT ...
-
bioRxiv - Cancer Biology 2021Quote: ... antibodies for 12 h at 4 °C followed by horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1/500; Invitrogen, Thermo Fisher Scientific, Inc.). After a PBS wash ...
-
bioRxiv - Molecular Biology 2021Quote: ... diluted in primary buffer for two overnights at 4°C followed by incubation in a donkey anti-goat Alexa 488-conjugated secondary antibody (A11055, 1:200; Life Technologies, Madison, WI) diluted in secondary buffer for three hours at room temperature ...
-
bioRxiv - Immunology 2020Quote: ... PBMC were collected and washed twice (400 × g, 8 min, 4°C) with cold PBS containing 1 mM UltraPureTM EDTA (Invitrogen, ThermoFisher, Basel Switzerland). In order to remove platelets ...
-
bioRxiv - Microbiology 2021Quote: ... 0.4 µ mol of each primer 27F and 1492R (Lane, 1991) (Table 1) and 0.5 U of DreamTaq polymerase (Fisher Scientific, Waltham, MA). All PCR reactions were carried out in a G-Storm GS2 thermocycler (G-Storm ...
-
bioRxiv - Immunology 2020Quote: Cells were plated and allowed to adhere to the cover glass of a 4-well or a 1-well chamber (Nalge Nunc International, Naperville, IL) precoated with Fibronectin (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... Internalization of Alexa Fluor 488-conjugated human α-syn PFFs (1 μM) and epidermal growth factor (EGF; 4 μg/mL; Thermo Fisher Scientific) were assessed similarly ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
-
bioRxiv - Synthetic Biology 2023Quote: ... media was aspirated from each well and replaced with a solution of 4% paraformaldehyde (PFA) created fresh by fixing a 1 mL 16% (w/v) PFA ampule (ThermoFisher Scientific, catalog #28906) with 3 mL PBS ...
-
bioRxiv - Neuroscience 2023Quote: ... and were incubated overnight at 4°C in polyclonal rabbit IgG fraction anti-Lucifer yellow (1:500, Life Technologies - Thermo Fisher Scientific, RRID:AB_2536190) in 0.3% PBS-T and 1% BSA ...
-
bioRxiv - Biochemistry 2023Quote: ... The BD5.1 bromodomain was dialysed at 4 °C overnight in a Slide-A-Lyzer® MINI Dialysis Device (2000 MWCO; Thermo Scientific Life Technologies) into HEPES buffer (20 mM HEPES ...
-
bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000 ...
-
bioRxiv - Cell Biology 2024Quote: ... Coverslips were blocked on ice with 1% BSA in 1x PBS for 30 minutes followed by incubation overnight at 4°C with anti HA-488 antibody (#26183, 16B12, Invitrogen, USA, 1:1000) and where applicable with Myc (#2278 ...
-
bioRxiv - Bioengineering 2024Quote: ... they were stained overnight at 4°C with phalloidin conjugated to Alexa Fluor 488 (1:250 dilution in PBS; Invitrogen, Waltham, Massachusetts, USA) and DAPI (1:10,000 dilution in PBS) ...
-
bioRxiv - Genetics 2024Quote: ... then incubated with 1:500 (4 µg/mL) dilution goat anti-rabbit IgG (H+L) AlexaFlour®488 antibody (Invitrogen, catalog # A-11008) in 2% NGS in 1X PBS for 1 hour ...
-
bioRxiv - Molecular Biology 2024Quote: ... Differentiation was induced at ∼85% confluency by switching to media containing 4:1 DMEM/M199 supplemented with 5% normal horse serum (Invitrogen; cat #: 26050-088). Following seven days of differentiation cells were treated with either ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were washed 3 times (10 min) and post fixed with 4% PFA followed by counterstaining of nuclei using DAPI (1:4000 for 10 min, ThermoFisher Scientific, Cat # 62248). Coverslips were mounted onto glass slides and direct fluorescence of mitotic cells and genomic instability visualized using a deconvolution microscope (ECLIPSE Ti2 Inverted Microscope ...