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Citations for Thermo Fisher :
2501 - 2550 of 8720 citations for Recombinant Human LDLR His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Purified F antigens were biotinylated using an EZ-link Sulfo-NHS -LC-Biotinylation kit (Thermo Fisher, cat#A39257) with a 1:1.3 molar ratio of biotin to F ...
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bioRxiv - Cancer Biology 2022Quote: ... biotinylated PCR product is bound and isolated using Dynabeads M-280 Streptavidin magnetic beads (Thermo Fisher Scientific, #11206D). Briefly ...
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bioRxiv - Genomics 2022Quote: ... and a 1:100 dilution of Anti-mouse CD34 biotinylated antibody (Thermo Fisher Scientific, Cat#: 13-0341-85) for 20min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... fibronectin was biotinylated by incubating 0.1mg/mL fibronectin with 0.5mM EZ-Link Sulfo-NHS-LC-Biotin (A39257, ThermoFisher) for 30 min at RT ...
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bioRxiv - Biochemistry 2019Quote: ... Biotinylated template RNA was incubated with 250 ng streptavidin or 50 μl streptavidin-agarose resin (Thermo Fisher Scientific). Reactions were stopped with 10 mM EDTA ...
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bioRxiv - Molecular Biology 2020Quote: ... EdU-genomic DNA was then biotinylated using the Click reaction and purified using Streptavidin T1 magnetic beads (Invitrogen). Libraries for Illumina sequencing were constructed using TruSeq ChIP Library Preparation Kit (Illumina) ...
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bioRxiv - Cancer Biology 2021Quote: ... Detection of the biotinylated probe was done using the Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Fisher 89880).
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Bat influenza vectored NS1-truncated live vaccine protects pigs against heterologous virus challengebioRxiv - Microbiology 2020Quote: ... plates were washed and incubated with 50 μl of 0.5 μg/ml biotinylated-porcine IFN-γ antibody (Invitrogen) for 2h at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... were biotinylated using the EZ-Link Sulfo-NHS-LC-biotinylation kit according to manufacturer instructions (Thermo Fisher Scientific) and used in the assay at concentrations of 1.25 ...
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bioRxiv - Cell Biology 2019Quote: ... 50 pmol of in vitro transcribed RNA was biotinylated using RNA 3’ End biotinylation Kit (Thermo Fisher Scientific) overnight at 16°C ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... rshACE2 and deglyco-rshACE2) were biotinylated using the EZ-Link Sulfo-NHS-LC-Biotin kit (Thermo Fisher Scientific). Excess sulfo-NHS-LC-biotin was quenched by adding Tris-HCl buffer (800 mM ...
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bioRxiv - Immunology 2021Quote: ... was biotinylated using the EZ-Link Sulfo-NHS-LC-Biotinylation kit according to the manufacturer’s instructions (Thermo Scientific). Biotinylated ovalbumin was conjugated to streptavidin-BV711 (BD biosciences ...
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bioRxiv - Microbiology 2020Quote: ... Biotinylated hACE2-Fc was prepared using EZ-Link™ Sulfo-NHS-LC-LC-Biotin kit (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: ... was biotinylated using the EZ-Link Sulfo-NHS-LC-Biotinylation kit according to the manufacturer’s instructions (Thermo Scientific). Biotinylated Ovalbumin was conjugated to streptavidin-BV711 (BD biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... pH 7.2) and surface proteins were biotinylated using 0.5 mg/ml Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific) in PBS for 40 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with primary antibodies and subsequently with the corresponding biotinylated secondary antibodies (1:1000 Goat anti-rabbit, Invitrogen). Signal amplification was performed using the ABC complex (Vectashield) ...
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bioRxiv - Immunology 2022Quote: ... was biotinylated using the EZ-Link Sulfo-NHS-LC-Biotinylation kit according to the manufacturer’s instructions (Thermo Scientific). Biotinylated ovalbumin was conjugated to streptavidin-BV711 for single-cell sorts (BD biosciences ...
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bioRxiv - Microbiology 2022Quote: ... biotinylated SARS-CoV-2 Spike trimer (Hexapro) was incubated with red streptavidin-fluorescent beads (Molecular Probes, Eugene, OR) for 2h at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein ligands (Red1288-345 and Iss1) were biotinylated using EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific). Biotin-labeled proteins were immobilized on the streptavidin (SA ...
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bioRxiv - Bioengineering 2022Quote: ... Capture antibodies were biotinylated using an EZ-Link™ micro Sulfo-NHS-biotinylation kit (ThermoFisher Scientific, Waltham, MA). All RNA molecular beacons (MBs ...
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bioRxiv - Biophysics 2024Quote: ... CALHM2 and CALHM4 proteins were chemically biotinylated using amine-reactive EZ-Link™ NHS-PEG4-Biotin (ThermoFisher Scientific). For this purpose ...
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bioRxiv - Microbiology 2023Quote: ... biotinylated Pol II CTD mimic peptide (20 µg) were immobilised on 10 µl streptavidin agarose resin (Thermo Scientific) for 2 hours at 4 °C in binding buffer (10 mM HEPES [PAA catalogue no ...
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bioRxiv - Cell Biology 2023Quote: ... The neurons were washed in ice-cold PBS and biotinylated with 0.2 mg/mL biotin (Thermo Fisher, #21335) at 4°C for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... The biotinylated RNA-RNA complexes were pulled down by incubation with Dynabeads M-280 Streptavidin (Thermo Fisher Scientific) for 4 hr at 40C ...
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bioRxiv - Genomics 2023Quote: ... a biotinylated pull-down step was performed by adding 30 µl of streptavidin C1 MyOne Dynabeads from Invitrogen to 300 µl of binding buffer (10 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were cell-surface biotinylated with 0.1 mg/ml Sulfo-NHS-LC Biotin (Thermo Fisher Scientific, Waltham, MA) in HBSM (20 mM HEPES pH 7.2 ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested and biotinylated peptides were captured (80 μl Pierce™ Streptavidin UltraLink™ Resin, ThermoFisher Scientific), resin was washed ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated in primary antibody at 4°C overnight (biotinylated AT8, 1:500, MN1020B, ThermoFisher Scientific). The biotin signal was amplified using an ABC Elite kit (1:400 ...
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bioRxiv - Genomics 2023Quote: ... Biotinylated DNA was then enriched with 5 µL pre-washed Streptavidin C1 beads per sample (Life Technologies, 65001) in biotin binding buffer (5mM Tris-HCL pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... 2 μg/μl biotinylated dsRNA and unlabeled control were incubated with 55 mM streptavidin (ThermoFisher, cat. no. 211122) dissolved in nuclease-free water with 20 mM potassium phosphate pH 6.5 ...
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bioRxiv - Bioengineering 2023Quote: ... Bound SiRPα-Fc was detected with a secondary biotinylated rabbit anti-IgG Fc monoclonal antibody (Thermo Fisher Scientific) followed by HRP-conjugated streptavidin (Thermo Fisher Scientific) ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... Cells were then biotinylated with EZ-Link™ Sulfo-NHS-SS-Biotin (0.3 mg/ml, Thermo Fisher Scientific) for 30 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The biotinylated anti-GFP nanobody was added to Pierce High Capacity Streptavidin Agarose Resin (Thermo Scientific, cat. #20359) equilibrated in GF1 buffer and allowed to incubate with the resin overnight at 4 °C ...
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bioRxiv - Genomics 2022Quote: ... and biotinylated RNA was enriched with streptavidin-coated beads (DYNAL™ Dynabeads™ M-280 Streptavidin, ThermoFisher 11205D). qRT-PCR evaluation was performed also with the primers suggested by Fuchs et al ...
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bioRxiv - Genomics 2022Quote: ... the RNA collected in each time point was biotinylated using the EZ-Link biotin HPDP kit (ThermoFisher, 21341) and biotinylated RNA was enriched with streptavidin-coated beads (DYNAL™ Dynabeads™ M-280 Streptavidin ...
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Preference of CAMSAP3 for expanded microtubule lattice contributes to stabilization of the minus endbioRxiv - Biophysics 2023Quote: Fluorescently labeled tubulin and biotinylated tubulin were prepared by adding Alexa Fluor 488 NHS ester (A20000; Thermo Fisher), Sulfo-Cy5 NHS Ester (13320 ...
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bioRxiv - Immunology 2022Quote: ... was biotinylated using the EZ-Link Sulfo-NHS-LC-Biotinylation kit according to the manufacturer’s instructions (Thermo Scientific). Biotinylated Ovalbumin and RBD were conjugated to streptavidin-BV711 (BD biosciences ...
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bioRxiv - Immunology 2023Quote: ... mAbs or ACE2 protein were first biotinylated using the EZ-Link Sulfo-NHS-Biotinylation Kit (Thermo Fisher Scientific) followed by removal of unreacted biotinylation reagents using a Zeba Spin Desalting Column (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... purified SARS-CoV-2 Spike and RBD were biotinylated using the EZ-Link NHS-Biotin (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Probes were freshly prepared for each experiment by incubation of mono-biotinylated RH5ΔNC with streptavidin-PE (S866, Invitrogen) or streptavidin-APC (405207 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by goat anti-rabbit biotinylated secondary antibody and streptavidin conjugated with Alexa Fluor 488 (Invitrogen, Carlsbad, CA). For double labeling with FOXP1 ...
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bioRxiv - Biophysics 2024Quote: ... Ligate the Cy5 Cy7 dual-labeled DNA with the biotinylated fragment using T4 DNA ligase (Thermo Fisher EL0011), and purify the ligated DNA by agarose gel extraction.
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bioRxiv - Synthetic Biology 2024Quote: Purified RECQL5 and sfGFP proteins were biotinylated using EZ-Link Sulfo-NHS-LC-Biotinylation Kit (Thermo Scientific #21435) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... Control Drosophila melanogaster biotin-tagged kinesin-1 motors were labeled with streptavidin-conjugated Qdot 655 (Life Technologies, Carlsbad, CA) at a 1:4 motor:Qdot ratio as previously described [16 ...
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bioRxiv - Cell Biology 2020Quote: ... K16-flag protein (C-terminally tagged) was made using the pcDNA™3.1 Directional TOPO™ Expression Kit (ThermoFisher) using primers for the mouse Krt16 gene with flag peptide sequence on the 3’ end (Supplemental Table 1).
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bioRxiv - Cell Biology 2020Quote: ... The pDONR201 clones were transferred into GFP or SFB tagged DEST vectors using the Gateway LR cloning system (Invitrogen). KDM5AΔPID deletion mutant was generated by PCR in pDONR201 vector following standard cloning method and then subcloned into Gateway destination vector derived from pcDNA5/FRT/TO plasmids containing the NLS and SFB- or GFP-tagged in N-terminal ...
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bioRxiv - Cell Biology 2020Quote: ... GFP or luciferase tagged versions of the proteins were generated using the Gateway recombination cloning procedure (Invitrogen, Carlsbad, CA) and custom-made vectors.
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids with 3xFLAG tagged SARS-CoV-2 proteins were transiently expressed in BEAS-2B cells using Lipofectamine 3000 (ThermoFisher) transfection according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: HEK293T cells grown on 100-mm dishes were cotransfected with Flag-tagged ASK1_KR and PKA or empty vector using Lipofectamine 2000 (Invitrogen). Twenty-four hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Transferrin (Tf) and Epidermal Growth Factor (EGF) tagged with Alexa Fluor 488 was obtained from Invitrogen (T13342 and E13345). Dako fluorescent mounting medium from Agilent (S3023 ...