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Citations for Thermo Fisher :
2501 - 2550 of 10000+ citations for Recombinant Human CD52 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Raw MS Data were searched against the human SwissProt protein database (Version November 2020) with Proteome Discoverer 2.4 (ThermoFisher Scientific, Bremen, Germany) using the following parameters ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells in 35-mm dishes were transfected with 100 pmol of each siRNA in the Stealth siRNA library targeting 154 human nuclear proteins (Invitrogen–Thermo Fisher Scientific) using Lipofectamine RNAiMax (Invitrogen–Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... operated by UNICORN software version 5.11 (Build 407) using HiTrap Protein A columns (Cytiva) for full length human mAbs and CaptureSelect CH1-XL MiniChrom columns (Thermo Fisher Scientific) for Fab fragments ...
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10428) and 1-Step Human Coupled IVT Kit – DNA (#88882) were obtained from Thermo Fisher Scientific Inc. ...
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bioRxiv - Immunology 2022Quote: ... All S constructs were verified by Sanger sequencing and the protein was subsequently produced in human embryonic kidney (HEK) 293F cells (Thermo Fisher Scientific) and purified as previously described (4 ...
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bioRxiv - Neuroscience 2022Quote: ... similarly as described previously.[60] MS data were analyzed using an in-house maintained human protein sequence databases using SEQUESTTM and Proteome DiscovererTM (Thermo Fisher Scientific). The mass tolerances of a fragment ion and a parent ion were set as 0.5 Da and 10 ppm ...
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bioRxiv - Immunology 2023Quote: Plasmids coding for the soluble S ectodomain proteins as well as the RBD of human coronaviruses were transfected into Freestyle293F cells (Thermo Fisher R79007). Per 1 L of Freestyle 293F cells ...
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bioRxiv - Neuroscience 2023Quote: ... Human brain tissue was homogenised with a Dounce homogeniser and placed in a low protein binding 15mL tube (Thermo Fisher Scientific, 30122216) containing 10mL 1X artificial CSF (pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: Plasmids encoding human ZFP36 proteins TTP and Brf1: human ZFP36 and ZFP36L1 open reading frames were amplified from the ORFeome cDNA clone library (Invitrogen, Life Technologies) by Phusion PCR (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: MS/MS searches were carried out using SEQUEST search algorithms against a Uniprot database for humans (supplemented with exogenous sequence for anticipated protein constructs) using Proteome Discoverer (Version 3.0, Thermofisher Scientific, Bremen, Germany). Oxidation of methionine and N-terminal protein acetylation were included as dynamic modifications and carbamidomethylation of cysteine was set as a static modification ...
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bioRxiv - Cancer Biology 2021Quote: ... human BNIP3 (Hs00969291_m1) and human 18S (Hs99999901_s1) were purchased from Applied Biosystems. 18S served as an internal control ...
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bioRxiv - Microbiology 2022Quote: Human monoclonal antibodies were produced recombinantly in human Expi293F cells (Life Technologies) as described before (83) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All ACE2-Fc constructs were produced in Expi293F cells (Thermo Fisher A14527) in Gibco Expi293 Expression Medium at 37°C in a humidified 8% CO2 incubator rotating at 130 rpm ...
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bioRxiv - Genomics 2022Quote: ... and staining was performed in the presence of Fc block (eBioscience/ThermoFisher, True-Stain Monocyte Blocker (Biolegend) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were blocked with Fc block (CD16/32, Invitrogen, 14-0161-82) for 20 minutes on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... and aliquots were incubated with Fc Block (ThermoFisher Scientific 14-9161-71) 20 minutes at 4°C ...
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bioRxiv - Immunology 2019Quote: ... containing 10% FCS (PAA) and 1% Penicillin-Streptomycin-Glutamine (Thermo Fisher Scientific). Medium was exchanged after 3 hours and cells were stimulated.
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bioRxiv - Genomics 2020Quote: ... washed twice in FC buffer and resuspended in Sytox Blue (Thermo Scientific) containing FC buffer for live/dead discrimination according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 1% penicillin-streptomycin and 10% fetal calf serum (FCS, Gibco) in an incubator (37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Monocytes were immersed in 45% fetal calf serum (FCS, 10500 ThermoFisher Scientific), and 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS, Thermo Fisher Scientific) and 1% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... FCS and L-Glutamine were all purchased from Gibco (Thermo Fisher Scientific). The following chemicals were obtained from Roth ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with 10% FCS (Eurobio) and 1% Non-Essential Amino Acids (Gibco). Primary astrocytes were obtained from E17 rat embryos following (Etienne-Manneville ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% fetal calf serum (FCS; ThermoFisher, 10499044 [Lot Number 08G8293K]) at 37°C with 5% CO2 ...
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SIMON, an automated machine learning system reveals immune signatures of influenza vaccine responsesbioRxiv - Immunology 2019Quote: ... filtered through 0.1um spin filter with 20uL/sample of Fc block (ThermoFisher) for 30min at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... in MLNL medium (low-glucose DMEM/1% FCS, Thermo Fisher Scientific 21885025). At day 2 ...
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bioRxiv - Physiology 2019Quote: ... On day 0 media was additionally supplemented with 10% FCS (Gibco#10270106). The hPCLS were treated with 10ng/ml recombinant human TGFß 1 protein (R&DSystems#240-B-010 ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% v/v (FCS) and 2 mM L-glutamine (Gibco). Where indicated ...
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bioRxiv - Immunology 2020Quote: ... and optical densities were acquired with a Multiskan™FC (Thermo Scientific).
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bioRxiv - Immunology 2020Quote: ... after which staining was inhibited by adding 10% FCS (10270-106, Gibco). Stained cells were washed in RPMI/PS prior to counting and addition to macrophages ...
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bioRxiv - Molecular Biology 2021Quote: ... These cells were cultured in DMEM containing 10% FCS (Thermo Fisher Scientific) and 1% penicillin-streptomycin (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... Absorbance was measured at 405 nm using a Multiskan FC (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... for 2h at RT on a Multiscan FC Plate Reader (Thermo Scientific) at medium shaking speed ...
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bioRxiv - Immunology 2020Quote: ... and 10 % V/V heat-inactivated foetal calf serum (Hi FCS, Gibco). On day 1 ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% FCS and 1% penicillin/streptomycin (#P433, Thermo Fisher Scientific) at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... with 10% v/v heat inactivated foetal calf serum (HI-FCS, Gibco), 1 mM sodium pyruvate and 1X non-essential amino acids (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... and HCC70 were cultured in an RPMI medium containing 10% FCS (Gibco), 2 mM glutamine ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% foetal calf serum (FCS) (Thermo Fisher Scientific: 10902-096) and 100U/ml of each penicillin and streptomycin (P/S ...
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bioRxiv - Immunology 2022Quote: ... Culture supernatant was then incubated with CaptureSelect IgG-Fc resin (Thermo Scientific) and eluted from the resin using 0.1 M glycine ...
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bioRxiv - Immunology 2022Quote: ... at 106cells/mL in complete RPMI medium supplemented with 10% FCS (Gibco), 50 μM 2-mercaptoethanol ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 10% heat inactivated fetal calf serum (FCS, Thermo Fisher, USA), 50 U/mL of penicillin (Ozyme ...
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bioRxiv - Microbiology 2022Quote: ... from Gibco supplemented with 10% heat inactivated fetal calf serum (FCS, Thermo Fisher, USA), 50 U/mL of penicillin (Ozyme ...
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bioRxiv - Immunology 2022Quote: ... The ΔeOD-GT8 Fc was conjugated with Biotin (Thermo Fisher Scientific A30010) and then stained with BV605 (BioLegend 405229).
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bioRxiv - Bioengineering 2022Quote: ... 20 mL of the perfluorinated amine FC-3283 (Acros Organics, Geel, Belgium) was added to each culture as an underlay ...
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bioRxiv - Cell Biology 2022Quote: ... were maintained in DMEM medium supplemented with 10% heat-inactivated FCS (Gibco) and 100 U/ml penicillin/streptomycin (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... Samples were stained in FACS buffer (PBS containing 2% FCS (Thermo Fisher) and 2 mM EDTA (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... HFF were maintained in DMEM supplemented with 10% FCS (GIBCO ref # 10270106).
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bioRxiv - Immunology 2024Quote: ... the anti-mouse IgG Fc cross-adsorbed secondary antibody (PE, Thermo Fisher) was added to detect the anti-rhesus antibodies with fluorescence ...
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bioRxiv - Microbiology 2023Quote: ... Polyclonal goat anti-pig immunoglobulin G (IgG) Fc Secondary Antibody HRP (Invitrogen by Thermo Fisher Scientific ...