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Citations for Thermo Fisher :
2501 - 2550 of 8309 citations for Rat TRPV1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Approximately two million colonies were scraped from plates and pooled and plasmid DNA was purified using PureLink® HiPure plasmid filter Midiprep kit (Invitrogen). The resulting plasmid library was transformed into TRy1392 pdr5∆ yeast strain ...
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bioRxiv - Microbiology 2024Quote: ... transfections were performed by introducing 20 μg of targeting plasmid and 60 μg of linearised repair template (purified using Pure-Link™ HiPure Plasmid Midiprep Kit, Invitrogen) into ∽108 Percoll-enriched schizonts by electroporation using an Amaxa 4D Nucleofector X (Lonza) ...
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bioRxiv - Developmental Biology 2024Quote: ... 293 cells were transfected with plasmids encoding His-tagged recombinant proteins as described in the Plasmids section using lipofectamine 2000 (Invitrogen, #11668019) following the manufacturer’s instructions (four 100 mm plates each plasmid) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 μg dual sgRNA plasmids or 1 μg sgRNA plasmids and 1 μg homologous recombination donor using Neon™ NxT Electroporation System (Invitrogen). After 48 hr ...
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bioRxiv - Immunology 2024Quote: ... 18 μg of pMDLg/p.RRE Gag/Pol plasmid and 18 μg of pRSV.Rev plasmid alongside 15 μg of transfer vector plasmid encoding for CAR of interest using Lipofectamine 2000 (Thermo Fisher Scientific) and Opti-MEM (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 µg of each plasmid (gagpol, vsv-g, and transfer plasmid) were combined with 2 ml Opti-MEM I Reduced Serum Medium (Gibco 31985070) and 90 µl MirusBio TransIT-LT1 Transfection Reagent (Mirus MIR2306 ...
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bioRxiv - Biophysics 2024Quote: ... HEK293T cells were co-transfected with 200 ng pCAGEN plasmid for TDP-43 and 1000 ng pCAGEN plasmid for the other protein using Lipofectamine 3000 Transfection Reagent (Invitrogen, L3000001) in 12-well plates ...
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bioRxiv - Immunology 2024Quote: ... pMDLg/p.RRE (18 μg) packaging plasmids and 15 μg of expression plasmids were mixed and transfected into 293T cells using Lipofectamine 3000 (Invitrogen #L3000150) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: The HEK293T packaging cells were transfected with OSA-expressing plasmids with lentiviral packaging plasmids using Lipofectamine Reagent 2000(Thermo Fisher Scientific). After 48 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... the SunTag plasmids and HMGB1-BFP (2 dishes) or Smad3-BFP plasmid (2 dishes) were simultaneously transfected with Lipo3000 transfection reagent (ThermoFisher Scientific). One dish of cells transfected with HMGB1-BFP ...
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bioRxiv - Molecular Biology 2023Quote: Extrachromosomal arrays were introduced by injection of 10 ng/μl plasmid DNA purified using the PureLink™ Quick Plasmid Miniprep Kit (Invitrogen) into the gonad of 1 DOA IR1284 (zcIs14[myo-3p::mtGFP] ...
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bioRxiv - Microbiology 2023Quote: ... The following day each well was co-transfected with 1 µg of replicon-encoding plasmid and 3 µg of C-prM-E-encoding plasmid using Lipofectamine 3000 (Cat# L3000-015; ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... were co-transfected with plasmids encoding GFP-nsP3 fusions and the integrase-encoding plasmid using Lipofectamine™ 3000 according to the manufacturer’s recommendations (ThermoFisher Scientific). Clones of blasticidin-resistant ...
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bioRxiv - Genomics 2023Quote: ... cells at 80–90% confluency were co-transfected with 0.25 µg of single-sgRNA (or 4sgRNA) plasmids and 0.25 µg of dCas9-VPR plasmids using Lipofectamine 3000 (Thermo Fisher Scientific). Three days post-transfection ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 5 ml of the culture was pelleted by centrifugation and plasmid DNA was isolated using a GeneJET Plasmid Miniprep Kit (Thermo Scientific). The plasmid sequence was verified using BigDye 3.1 sequencing chemistry and an ABI3130 Genetic Analyzer ...
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bioRxiv - Molecular Biology 2023Quote: ... was used at a 3:1 ratio with 1 µg total plasmid (500 ng nLuc plasmid + 500 ng pGL4.13) in 100 µL in Opti-MEM (Thermo Fisher # 31985062). For 96-well plates ...
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bioRxiv - Cell Biology 2023Quote: ... co- transfected with lentiviral packaging plasmids (pCEP4-tat, pHEF-VSVG and pNHP) and lentiviral expression plasmids using Lipofectamine 2000 (Invitrogen, 11668019) as previously described50 with the following modifications ...
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bioRxiv - Developmental Biology 2023Quote: SOX2:GFP ESCs were plated into a gelatinised 6-well plates in SL and lipofected with the linearized Gata6:mCherry-Hygromycin plasmid and the CRISPR plasmid using Lipofectamine2000 (ThermoFisher 11668019) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The mESCs were co-transfected with the 1.66 μg of the two sgRNA nickase-Cas9 plasmids and 0.83 μg of the HDR plasmid using the Lipofectamine LTX and Plus Reagent (Invitrogen, 15338-100) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The mESCs were co-transfected with the 1.66 μg of the two sgRNA nickase-Cas9 plasmids and 0.83 μg of the HDR plasmids using the Lipofectamine LTX and Plus Reagent (Invitrogen #15338-100). Transfected cells were first selected by 1 μg/ml puromycin for 2 days and subsequently selected by 250 μg/ml hygromycin for 6 days ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviral packaging plasmids (pCMV-D8.9 and pCMV-VSVG) and reporter plasmids were transfected into HEK-293T cells using Lipofectamine 3000 (ThermoFisher cat#L3000001). Virus-containing supernatant was harvested at 48 and 72 hours after transfection and viral particles were concentrated with Lenti-X Concentrator according to the manufacturer’s instructions (Takara Bio cat#631232) ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNA from either wild type dengue plasmid or mutated dengue plasmid were quantified using a High Sensitivity Range Qubit (Thermo Fisher). Library preparation was carried out with the Nextera XT DNA Library Prep kit (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... HD patient iPSC-derived astrocytes were transiently transfected with 2 μg hFAN1 plasmid and control plasmid (pTT5 vector) by Lipofectamine 3000 (Invitrogen; L3000008) every week ...
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bioRxiv - Biochemistry 2024Quote: ... 300 ng of WT or CNNC mutant miRNAs with 50 ng of non-CNNC mir-24 plasmids) and Drosha plasmids (800 ng per well) was performed in 12-well cell culture plates using Lipofectamine2000 (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... COS-7 cells were transfected with reporter plasmid construct together with pRL-TK Renilla luciferase plasmid by Lipofectamine™ 2000 (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... transfer plasmids were co-transfected with GAG/POL and VSVG plasmids into 293T cells using Lipofectamine™ 3000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The co-culture was transfected with the 8-plasmid set (2 μg of each plasmid) using Lipofectamine 2000 transfection reagent (Fisher Scientific). At 24 h post transfection ...
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bioRxiv - Synthetic Biology 2024Quote: ... Libraries were aliquoted in 10 mL (OD600 ∼ 0.2) and re-grown for 2-3 h to extract plasmids using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) for sequencing and for subcloning into the expression strain ...
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bioRxiv - Molecular Biology 2024Quote: ... transfer plasmids were co-transfected with GAG/POL and VSVG plasmids into 293T cells using Lipofectamine™ 3000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Three to six transformed colonies were cultured in 5 ml of LB medium and plasmids were subsequently purified with a GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific). Purified plasmids were sequenced by Eurofins Genomics (Fig ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Large quantities of purified plasmids containing AsegIRs and AsegIR8a with verified sequences were obtained using the PureLinkTM HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific). cRNAs of AsegIRs and AsegIR8a were synthesized from NotI (Promega ...
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bioRxiv - Microbiology 2022Quote: ... Expi293F cells at 2 × 106/ml were transfected with a mixture of 1 ng of library plasmid with 1 μg of pMSCV as a junk plasmid per ml using ExpiFectamine (Thermo Fisher Scientific). Twenty-four hours after transfection ...
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bioRxiv - Cell Biology 2019Quote: ... 5 µg of lentiviral plasmid packaging mix (24) and 5 µg of pooled sgRNA library plasmid was transfected using lipofectamine 2000 (ThermoFisher Cat# 11668019) and incubated for 2 days ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were co-transfected with 1 μg of the corresponding plasmids and 10 ng of a Cre recombinase plasmid using Lipofectamine 2000 (Thermo Fisher Scientific). Stable cell lines were selected after 2 days of growth by addition of 1 μg ml−1 puromycin ...
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bioRxiv - Immunology 2019Quote: ... The sgRNA was cloned pCas-Puro-U6 plasmid and the pCas-Puro-U6 plasmid Linear was obtained using the BbsI restriction enzyme (Thermo Fisher Scientific). The plasmids containing sgRNA were transfected into IPEC-J2 cells with GeneTran III (Biomiga ...
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bioRxiv - Microbiology 2019Quote: ... 293T cells in 6-well plates were transfected with 1.0 μg pMJ920 plasmids and 1.0 μg gRNA expression plasmids by Lipofectamine® 2000 Transfection Reagent (Invitrogen, Cat.11668-027) using the recommended protocols ...
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bioRxiv - Genomics 2020Quote: ... Correct assembly of the sgRNA into the backbone was confirmed by SANGER-Sequencing (Microsynth) and plasmids were isolated using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... 2.5 μg FLAG-hRpn13-expressing plasmid or 5 μg FLAG-hRpn131-279-expressing plasmid by using lipofectamine 3000 (Thermo Fisher Scientific, L3000015) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... these plasmids were transiently transfected into HEK293T cells with packaging plasmid psPAX2 and envelope plasmid pMD2.G using Lipofectamine 3000 transfection reagent (Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... sgRNA expressing plasmids were produced by cloning sgRNA oligonucleotides with the appropriate adaptor sequences (see Table S2) into plasmid pMCB306 after digesting the plasmid with restriction enzymes BstXI and Bpu1120I (Cat# FD1024, FD0094, Thermo Fisher Scientific). pMCB306 was a gift from Michael Bassik (Addgene plasmid #89360 ...
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bioRxiv - Plant Biology 2021Quote: ... Positive colonies were grown in LB overnight and then used for subsequent plasmid isolation (GeneJET Plasmid Miniprep Kit; Thermo Fisher Scientific, USA). About 150 pUC18R6KT-mini-Tn7T-Km recombinant plasmids were stored at −4°C ...
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bioRxiv - Microbiology 2021Quote: ... The resulting sub-cloning plasmids and the conjugative plasmid pVZ321 were cleaved with restriction enzymes XhoI and SalI (Thermo Scientific™, Germany), generating compatible restriction sites ...
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bioRxiv - Molecular Biology 2021Quote: ... Large quantities of purified plasmids containing ItypORs and ItypOrco with correct sequences were obtained using the PureLinkTM HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific). cRNAs of ItypORs and ItypOrco were synthesized from NotI (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... For lentivirus particle production these plasmids were recombined with pCDH-EF1a-Gateway-IRES-BLAST plasmids as described in [51] using LR Clonase II (Invitrogen/Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... 100 million HEK293T cells were seeded into a 15 cm2 plate with complete DMEM and transfected with 5 μg of lentiviral plasmid packaging mix and 5 μg of pooled sgRNA library plasmid using lipofectamine 2000 (ThermoFisher Cat# 11668019) and incubated for 2 days.
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bioRxiv - Molecular Biology 2022Quote: ... Cells were seeded at 1×105 cells per well in a 24 well multiplate and transfected with 800 ng plasmid DNA per well (400 ng of SaCas9 expression plasmid and 200 ng of each sgRNA plasmid) using Lipofectamine 2000 (Thermo Fisher Scientific) as according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated overnight at 37°C in shaker and the plasmid DNA was extracted using GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, Lithuania). As the final means of verification sequence of inserted fragment was verified through Sanger Sequencing employing M13-Fw and M13-Rs primers ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µg of each plasmid DNA (two plasmids expressing Cas9 and gRNAs) were mixed in 100 µl of reduced-serum medium OPTI-MEM (ThermoFisher Scientific, 31985062) then mixed with 15 µl of 1 mg/ml PEI ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmid DNA of the final constructs was isolated with a PureLink™ HiPure Plasmid Filter Midiprep Kit (Invitrogen™ /Thermo Fisher Scientific) according to the manufacturer’s manual ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmid DNA of the final constructs was isolated with a PureLink™ HiPure Plasmid Filter Midiprep Kit (Invitrogen™ /Thermo Fisher Scientific) according to the manufacturer’s manual ...