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Citations for Thermo Fisher :
2501 - 2550 of 10000+ citations for Pig Hemoglobin HB CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Lentivirus concentration kit (Cat. NO. 41101ES50) was acquired from Yeason. BCA protein assay kit (Cat. NO. FD2001) was obtained from Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Flow cytometry was used to evaluate the efficiency of differentiation to definitive endoderm at day 3 using the endoderm markers CXCR4 and c-KIT (Anti-human CXCR4 PE conjugate, Thermo Fisher, MHCXCR404,1:20; Anti-human c-kit APC conjugate, Thermo Fisher, CD11705 ...
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bioRxiv - Microbiology 2024Quote: ... Their concentrations were quantified by dsDNA (or ssDNA) HS Assay Kit or RNA HS assay kit (ThermoFisher Scientific, Waltham, MA, USA) with Qubit4.0 ...
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bioRxiv - Genetics 2024Quote: ... dsRNAs were transcribed using T7 Scribe Standard RNA IVT Kit (Cell Script) and purified with MEGAclear Transcription Clean-Up Kit (Thermo Fisher/Invitrogen). For dsRNA transfection ...
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bioRxiv - Cell Biology 2023Quote: ... The flow-through and wash from the TiO2 enrichment kit were then combined and enriched using the HiSelect Fe-NTA phosphopeptide enrichment kit (Thermo Fisher). The enriched phosphopeptides obtained by both methods were dried by speed vac and stored at −20 °C until analysis by mass spectrometry.
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bioRxiv - Cell Biology 2024Quote: ... All plasmids were purified with AxyPrep Plasmid Purification Miniprep Kit (Axygen, #AP-MN-P-250) and PureLink Quick PCR purification Kit (Invitrogen, #K310001). mMESSAGE mMACHINE® T7 Ultra Kit (Life Tech ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were tested for average fragment length using Agilent High Sensitivity DNA Kit (5067-4626) and concentration was tested using Qubit™ dsDNA HS kit (#Q32851, Invitrogen). DNA was stored at −20°C until used for sequencing ...
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bioRxiv - Cancer Biology 2022Quote: ... Bicinchoninic acid (BCA) assay kit for protein quantification and MitoProbe JC-1 Assay kit for flow cytometry were procured from Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... 5’-capped cRNA was prepared from the pGEMHE vector containing cDNA of opsin (see above) using an in vitro transcription kit (mMESSAGE mMACHINE Kit, Life Technologies). Typically ...
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bioRxiv - Cell Biology 2023Quote: RNAs extracted by either Trizol or RNeasy Plus Micro Kit were reverse transcribed to cDNAs using High-Capacity cDNA Reverse Transcription Kit (4368814; Applied Biosystems), and quantitative PCR was performed using DyNAmo Flash SYBR Green qPCR Kit (F415S ...
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bioRxiv - Microbiology 2023Quote: ... equal amounts of RNA from each sample were used for cDNA synthesis with the kit Revertaid First Strand cDNA synthesis Kit (Thermo Scientific). The obtained cDNA was diluted to 2-4 ng/µl for subsequent qPCR with the Sybr Green (Applied Biosystems ...
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bioRxiv - Biochemistry 2023Quote: ... 180 μg of mitochondrial proteins were subjected to a click reaction using a commercial kit (Click-iT Cell Reaction Buffer Kit; Thermo Fisher), with 40 μM Tetramethylrhodamine (TAMRA)-azide (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... These constructs were then used for in vitro translation using a HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA, 88881, Life Technologies). The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific) ...
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bioRxiv - Systems Biology 2023Quote: ... Libraries were purified using NucleoMag beads supplied in the library preparation kit and subsequently quantified using the Qubit 4 Fluorometer and the Qubit dsDNA HS assay kit (Invitrogen, #Q32854). The size distribution was checked using the TapeStation 4200 ...
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bioRxiv - Cancer Biology 2023Quote: ... Total mRNA was isolated with a PureLink RNA Mini Kit (life technologies) and cDNA synthesis was performed with a SuperScript III kit (Life Technologies), as per the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Library preparation for RNA sequencing was performed using the Ion Total RNA-Seq V2 kit and Ion Xpress RNA-Seq Barcode kit (Thermo Fisher). Library quality control was performed using the Ion Library Taqan Quantitation kit (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2023Quote: ... The fragment size of the library was assessed using the Agilent Bioanalyzer 2100 with the High Sensitivity DNA kit and was subsequently measured for the concentration by the High Sensitivity QuBit kit (Invitrogen, Q33230). Diluted libraries (4 nM ...
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bioRxiv - Developmental Biology 2023Quote: ... The T7-sgRNA PCR product was purified using Gel Extraction Kit (Omega) and used as the template for IVT of sgRNAs using the MEGAshortscript T7 kit (Life Technologies). Both the Cas9 mRNA and sgRNAs were purified using the MEGAclear kit (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting libraries were profiled using a High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical, #DNF-474) and measured using a Qubit dsDNA HS Assay Kit (Invitrogen, #Q32851) prior to pooling and sequencing using the Illumina NextSeq 500 platform using a custom primer and the High Output v2 kit (75Lcycles ...
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bioRxiv - Immunology 2023Quote: ... and cDNA was synthesized using PureLink™ RNA Mini Kit and High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher scientific, USA) respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA quality was assessed via Agilent Fragment Analyzer analysis using a High Sensitivity RNA analysis kit (DNF-472) and quantified using a Qubit 4 RNA BR kit (Thermo Fisher). RNA libraries were prepared with 500 ng total RNA ...
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bioRxiv - Immunology 2023Quote: ... LIVE/DEAD™ Fixable NearIR Dead Cell Stain Kit or LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit (Thermo Fisher) were used in all experiments to exclude dead cells ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% Triton-X and then assayed for triglyceride using a kit from Wako and cholesterol using a kit from Life Technologies.
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bioRxiv - Genomics 2023Quote: ... RNAs have been firstly quantified using Qubit™ RNA HS Assay Kit RNA HS Assay Kit (Thermo Fisher Scientific, Cat# Q32852). The RNA integrity was assessed using a Bioanalyzer ...
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bioRxiv - Microbiology 2023Quote: ... DNA from both size fractions were extracted using the MoBIO PowerWater DNA kit (MoBIO, USA) and quantified using the Qubit high-sensitivity dsDNA assay kit (ThermoFisher, USA). DNA was frozen at −20°C until sequencing ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was extracted using the QIAGEN DNeasy Blood and Tissue Kit following standard protocol and quantified using the Qubit HS DNA Assay kit and Qubit 2.0 fluorometer (Life Technologies Inc).
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bioRxiv - Bioengineering 2023Quote: Proliferative activity of 2D hydrogel cell cultures was measured using an EdU (5-ethynyl-2’-deoxyuridine) labeling kit (Click-iT™ EdU Cell Proliferation Kit for Imaging, Invitrogen). 5 μM EdU solution was dosed to cells 12 h prior to fixing with 4% paraformaldehyde ...
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bioRxiv - Microbiology 2024Quote: ... DNA was then quantified by Qubit with the dsDNA HS Assay kit (RNA HS Assay kit was used previously for the quantification of the RNA samples; Thermo Scientific) and about 100nM of each of the 5 subpools was pooled together into 1 sample for sequencing.
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription was performed on 1 ng of total RNA using a commercial kit (High Capacity RNA-to-cDNA Kit; Applied Biosystems) and Real-Time PCR amplification (CFX Opus 96 Real-Time PCR Instrument ...
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bioRxiv - Neuroscience 2024Quote: ... and protein concentration in the lysates was measured using a bicinchoninic acid protein assay kit (Micro BCA Protein Assay Kit, Thermo Scientific). Equal amounts (750 micrograms to 1 mg depending on the experiment ...
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bioRxiv - Developmental Biology 2024Quote: RNA was harvested from E13.5 mouse spinal cords using the Qiagen RNeasy Mini kit and complementary DNA (cDNA) libraries were prepared from each RNA isolate with the SuperScript III reverse transcriptase kit (ThermoFisher Scientific) and an anchored oligo-dT18 primer ...
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bioRxiv - Immunology 2024Quote: ... “Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit” and “Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit” were purchased and cells were stained according to kit instructions (ThermoFisher). DNA quantification was performed by staining with DAPI (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... Serum IL-6 and TNF-α concentrations were determined with enzyme-linked immunosorbent assay (ELISA) kits (Legend Max Mouse IL-6 ELISA Kit, BioLegend, and Mouse TNF Alpha Uncoated ELISA, Invitrogen, respectively). Serum noradrenaline was extracted with the use of a cis-diol–specific affinity gel ...
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bioRxiv - Microbiology 2024Quote: ... Plasmid DNA was purified using the GeneJet Plasmid Miniprep kit (Thermo K0503) or the Purelink HiPure Plasmid Midiprep kit (Invitrogen K210005). PCR was performed according to manufacturer directions using Q5 or OneTaq DNA Polymerases (NEB ...
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bioRxiv - Genetics 2024Quote: ... XbaI for 3’ UTRs) were attached for digestion with Thermo Scientific FastDigest kit and for cloning with Rapid DNA Ligation kit (Thermo Scientific) into the pGL3-Promotor (Promega ...
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bioRxiv - Genetics 2024Quote: ... The PierceTM BCA protein assay kit (cat. # 23225) and the LiquiColor triglyceride test kit (cat. # 2100-225) were purchased from Thermo Fisher Scientific and Stanbio Laboratory ...
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bioRxiv - Cancer Biology 2021Quote: ... Library quality was assessed using the Agilent High Sensitivity DNA kit and libraries were quantified using the Qubit dsDNA HS Assay kit (Thermo Fisher Scientific). Libraries were pooled (seven to eight libraries/pool ...
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bioRxiv - Genetics 2021Quote: ... Fragment size analysis and quantification of ATAC-seq libraries were performed using an Agilent Technologies 2100 Bioanalyzer and high sensitivity DNA kit and the Qubit dsDNA HS Assay Kit (ThermoFisher Cat#32854). ATAC-Seq libraries were sequenced on an Illumina HiSeq 4000 to a depth of at least 100 million paired-end 75bp reads per library.
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bioRxiv - Microbiology 2019Quote: ... Purification of DNA extracts was carried out with the PowerClean Pro DNA Clean-up Kit (MO BIO Laboratories) and DNA was quantified with the Qubit dsDNA HS Assay kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Human IL-18 and TNFα were measured with the human IL-18 ELISA Kit (MBL, Woburn, Massachusetts) and human TNFα ELISA Kit (ThermoFisher, Waltham, Massachusetts) according to the manufacturers’ instructions using cell culture supernatant at a 1:5 dilution ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA quality was assessed using an RNA 6000 Pico Kit (Aligent, 5067-1513) and concentration determined using the Qubit™ RNA HS assay kit (Invitrogen, Q32852).
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bioRxiv - Neuroscience 2022Quote: ... RNA was purified using the RNeasy micro kit (#74004) and cDNA was generated with the High-Capacity Reverse Transcription Kit (Applied Biosystems #4374966). A negative control (-RT ...
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bioRxiv - Neuroscience 2021Quote: ... The chemiluminescent Western blot detection kit (SuperSignal West Femto Chemiluminescent Substrate) and ATP determination kit were from Thermo Scientific (Rockford, IL, USA). The ELISA Kit for rat/mouse insulin ...
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bioRxiv - Genomics 2020Quote: ... The oligonucleotides were subsequently transcribed in vitro using the manufacturer’s instructions (TranscriptAid T7 high yield transcription kit / MEGAscript SP6 transcription kit, both ThermoFisher Scientific, Waltham, USA). The gRNAs were purified ...
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bioRxiv - Immunology 2021Quote: ... The remaining cell suspension was used to isolate CD8+ T cells with the magnetic-bead-based positive selection kit: Dynabead’s positive CD8+ isolation kit (Thermo Fisher, Waltham, MA). It is important to use an isolation kit that allows separation of cells from the beads for the samples to be compatible with 10x’s single-cell preparation ...
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bioRxiv - Microbiology 2019Quote: ... Purified DNA and RNA from these four time points were quantified using Qubit dsDNA HS Assay Kit and Qubit RNA HS Assay Kit (Invitrogen, Carlsbad, CA), and also quantified using NEB Luna Universal qPCR Master Mix and Luna Universal One-Step RT-qPCR Kit (New England Biolabs ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA used was using QIAGEN® RNeasy Mini Kit and cDNA synthesised utilising the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). 10ng of cDNA was used with the designed primers (Supplementary Table S4 ...
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bioRxiv - Plant Biology 2020Quote: ... the fragments were cloned into entry vector pDONR207 and yeast expression vector pYesdest52 using Gateway BP Clonase II Enzyme Kit and LR Clonase II Enzyme Kit (Invitrogen, MA, USA), respectively.
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bioRxiv - Genetics 2019Quote: ... The linearized Cas9 plasmids were transcribed into mRNA using the T7 m MESSAGE Kit (Ambition, USA) and gRNA was synthesized using transcript Aid T7 High Yield Transcription Kit (Thermo Scientific, USA). Zebrafish embryos at the one-cell stage were co-injected with 20pg target gRNA and 300pg Cas9 mRNA ...
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bioRxiv - Developmental Biology 2020Quote: ... using the Agilent RNA 6000 Nano Kit and concentrations were assayed with the Qubit RNA Broad Range Assay Kit (Thermo Fisher Scientific). For the transcriptome analysis ...