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Citations for Thermo Fisher :
2501 - 2550 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... The bead-protein (Thermo Scientific Sera-Mag Speed Beads A and B ...
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bioRxiv - Cancer Biology 2024Quote: ... Mini Protein Gel (Thermofisher, XP04200BOX) for 1.5 hours at 125 V to separate the proteins ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by protein transfer (Invitrogen) in Tris-glycine buffer at 25V (90’ ...
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bioRxiv - Cancer Biology 2021Quote: ... YT and KHYG-1 cells was performed with the Pierce Cell Surface Protein Isolation kit (Thermo Scientific, cat#89881) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... centrifugation and resuspension in protein sample buffer (50mM Tris-HCl pH6.8, 1% SDS, 10% glycerol) followed by quantification using Qubit (Invitrogen). Following quantification ...
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bioRxiv - Molecular Biology 2020Quote: ... Each half of diluted lysate is incubated with 1/10 volume of antibody coated Protein G Dynabeads™ (Invitrogen) for 40 min at 4°C by end to end rotation ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... the membranes were probed with anti-TasA antibody (rabbit) used at a 1:20,000 dilution in Pierce Protein-Free (TBS) blocking buffer (ThermoFisher). For immunodtection of FloT-YFP ...
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bioRxiv - Immunology 2019Quote: ... A total of 1 mg cytoplasmic protein was incubated with 100 μL Dynabeads M-280 streptavidin (Thermo Fisher Scientific) conjugated with 5′-biotinylated siRNA or 100 bp linearized DNA ...
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bioRxiv - Molecular Biology 2019Quote: ... An aliquoted 1:10 dilution in water was quantified with Pierce BCA protein assay (ThermoFisher Scientific Cat. No. 23225). While the protein assay was incubating at 37°C in the dark ...
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bioRxiv - Cell Biology 2019Quote: ... Anti-PRG-1 antibody (Custom) or rabbit IgG was pre-coupled to protein A/G matrix (Thermo Scientific, 88802) and incubated with the supernatant of worm lysates for 4 h (Anti-PRG-1 with N2 lysates ...
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bioRxiv - Immunology 2021Quote: ... Concentration of eotaxin-1 and cytokines was normalized to the total protein amount assessed by BCA (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... Total protein extracts (100 µg per reaction) were incubated with or without 1 mM BMH (Thermo Fisher Scientific 22330) for 30 min at 30°C ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were reduced by the addition of 1/10 volume of 45 mM dithiothreitol (Pierce Thermo Scientific #20290) and incubation at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Tubes were incubated for 1 hour at 4°C and then protein A/G agarose magnetic beads (Thermo Scientific) were added for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by a 1 h incubation at room temperature with Dynabeads Protein G magnetic beads (Thermo Fisher Scientific, #10003D). Beads were washed three times with lysis buffer and eluted by boiling in SDS sample buffer ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-mouse IgG (1:5000) and detection of protein was conducted using ECL Western blotting reagents (Thermo Fisher). All Western blots are representative images from at least three biological replicates.
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bioRxiv - Immunology 2022Quote: FoxP3RBR-forkhead (19 μl, 0.25 mg/ml) was mixed with 20x Protein thermal shift dye (1 μl) (Thermo Scientific) in SEC buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were precleared by incubation for 1 hour at 4°C on a rotator with 10μl Protein G magnetic beads (ThermoFisher). The indicated antibody was incubated in 600μl IP buffer containing 1μl 10mg/ml acetylated BSA and 5μl Protein G magnetic beads for 4 hours with rotation at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were visualized by incubation in 1 in 50,000 dilution of HRP-conjugated goat anti-rabbit IgG (Life Technologies) for one hour followed by Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated-SARS-CoV-2 tri-S proteins were mixed with FITC-labelled NeutrAvidin beads (1 µm, Thermo Fisher Scientific) (1 µg of tri-S for 1 µl of beads) ...
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bioRxiv - Pathology 2021Quote: ... The supernatant was moved to a clean tube and incubated overnight with 0.5 μg of either goat anti-mouse KIM-1 or mouse anti-MUC1 (B27.29) antibodies and Protein G conjugated to Sepharose 4B (ThermoFisher). Beads were washed with 0.5 ml HEPES-buffered saline (HBS ...
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bioRxiv - Cell Biology 2019Quote: ... The surviving clones were screened for expression of the desired proteins in the presence of 1μg ml−1 doxycycline (Invitrogen). Expression of Myc-Ska3 was also induced with 1μg ml−1 doxycycline in the subsequent experiments.
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bioRxiv - Genetics 2020Quote: ... Whole cell protein samples were resolved by SDS-PAGE electrophoresis and analyzed by Western blot using anti-V5 (1:40,000) (Invitrogen) with goat anti-mouse HRP (1:40,000) ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... mCherry-fused Piezo1 protein was probed with rat monoclonal anti-mCherry antibody (Clone 16D7, ThermoFisher Scientific; 1:1,000 dilution); the native human Piezo1 channel was probed using a mouse monoclonal anti-Piezo1 antibody (Cat# NBP2-75617 ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 µl of an isolation buffer (50 mM ammonium bicarbonate, 1% SDS, 1x Protein Inhibitor Thermo Fisher Scientific, #78430) were added to each tube containing the pellet ...
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bioRxiv - Microbiology 2020Quote: ... 10 μL of purified proteins (∼1 mg/ml) were digested using Smart Digest Soluble Trypsin Kit (Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2021Quote: ... 160 U ml−1 RNAsin and protease inhibitors) and pre-cleared with Dynabeads Protein G beads (Thermo Fisher Scientific) for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... alongside aforementioned activating proteins and CellEvent™ Caspase-3/7 Green Detection Reagent (1:5000, ThermoFisher Scientific, Cat#C10423). Cells were imaged using the IncuCyte ZOOM System (Essen Bioscience ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1 µg/ml leupeptin with 1mM of PMSF immediately before use) for protein isolation or in Trizol (Invitrogen, US) for RNA isolation ...
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bioRxiv - Neuroscience 2024Quote: Hippocampal brain tissue was homogenized for 1 min in Tissue Protein Extraction Reagent (TPER®, Thermo Scientific, catalog # 78510) at 10% w/v with phosphatase and protease inhibitor cocktails (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... The SyPro orange dye was added to a final concentration of 1 X Protein Thermal Shift™ Dye (Thermofisher) in the reaction mixture ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected using a 1:10,000 dilution of fluorescent IRDye 800CW goat anti-mouse IgG (925-32210; Thermofisher) or IRDye 680RD Goat anti-Mouse IgG Secondary Antibody (925-68070 ...
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bioRxiv - Microbiology 2024Quote: Recombinant soluble spike and RBD proteins from Wuhan-1 and BA.1 SARS-CoV-2 strains were expressed as described 58,59 Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Biochemistry 2024Quote: To perform DSF, 5 µM protein was mixed with substrate (0, 1, or 15 mM) and 2.5x SYPRO Orange dye (ThermoFisher), and made up to a total volume of 50 µl with DSF buffer (50 mM Tris pH 8 ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by secondary antibody for 1 hour and processed for protein detection using SupersignalTM West Pico PLUS (Thermo Scientific) or Immobilon Western chemiluminescent substrate (Millipore Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... the slices were incubated with anti-green fluorescent protein rabbit IgG primary antibody (1:1000; Molecular Probes, OR, USA) and anti-Tyrosine Hydroxylase (TH ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with either horseradish peroxidase (HRP)-conjugated anti-c-myc antibody (1:5,000) (Thermo Fisher Scientific) or HRP-conjugated anti-GFP antibody (1:5,000 ...
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bioRxiv - Microbiology 2022Quote: ... 1-10ul of acidified protein digest was loaded onto a 20mm x 75um Pepmap C18 trap column (Thermo Scientific) on an Ultimate 3000 nanoRSLC HPLC (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were incubated in shake tubes over 1 h at 4°C with recombinant protein G–Sepharose (Invitrogen). The Sepharose was washed with RIPA lysis buffer and eluates were obtained through an addition of Laemmli Buffer.
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bioRxiv - Cell Biology 2023Quote: Protein extraction was performed by adding 500µL of TEAB 100mM lysis buffer with 1% SDS (Thermo Fisher, Waltham, USA) to each sample ...
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bioRxiv - Synthetic Biology 2023Quote: ... proteins were transferred onto a 0.2 μM PVDF membrane (Cytiva) using Power Blotter 1-Step™ Transfer Buffer (Invitrogen) for 10 minutes at 1.3 A ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were immunostained by primary antibodies like neuronal nuclear protein (Neu-N) (Invitrogen, California, USA, 1:500 dilution) (Gusel’Nikova et al. ...
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bioRxiv - Cell Biology 2023Quote: ... 1:1000 secondary antibody Alexa488 for E protein labeling (A-21121, goat anti-mouse, Thermo Fisher, Waltham, MA, USA), and 1:1000 Hoechst-33342 ...
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bioRxiv - Plant Biology 2023Quote: ... Separated proteins were transferred to a PVDF membrane and probed with monoclonal mouse anti-Myc (1:3,000; R950-25, Thermofisher), polyclonal rabbit anti-GFP (1:3,000 ...
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bioRxiv - Plant Biology 2023Quote: ... Separated proteins were transferred to a PVDF membrane and probed with monoclonal mouse anti-Myc (1:3,000; R950-25, Thermofisher), polyclonal rabbit anti-GFP (1:3,000 ...
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bioRxiv - Immunology 2023Quote: ... YT and KHYG-1 cells was performed with the Pierce Cell Surface Protein Isolation kit (Thermo Scientific, cat#89881) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... the sections were incubated with SARS-CoV-2 antibody against the nucleocapsid (N) protein (ThermoFisher MA536086; 1:28,000 dilution) for 15min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining was performed at 4 °C for Scribble and CHIKV proteins using anti-Scrib (1:500, Invitrogen PA5-54821), anti-E2 (1:500 ...
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bioRxiv - Bioengineering 2024Quote: ... Protein (10 μg) from each sample was combined with 1X Bolt LDS Sample Buffer (1:4, Invitrogen catalog #B0007) and 1X Bolt Reducing Agent (1:10 ...
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bioRxiv - Genetics 2024Quote: ... and Flag tagged (NLLRR) proteins were detected with 1/5,000 dilution of the anti-Myc and anti-Flag (cat# MA1-91878, Thermofisher) primary antibodies ...