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Citations for Thermo Fisher :
2501 - 2550 of 10000+ citations for 7 Oxabicyclo 4.1.0 hepta 2 4 diene 1 3 4 trifluoro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged as aggregates every 3-4 days until they reach 60-70% confluency with 0.5mM EDTA diluted in PBS (Thermofisher, Canada. Catalog#15575020).
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bioRxiv - Microbiology 2020Quote: ... The grids were blotted for 3-4 s and plunged into an ethane/propane mixture using a vitrification apparatus (Vitrobot, Thermo Fisher Scientific). For Fab 4G2-treated VLP EM sample preparation ...
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bioRxiv - Cancer Biology 2020Quote: ... Mice were immunized by injection of OVA coupled to the hapten 4-hydroxy-3-nitrophenylacetyl (NP-OVA) precipitated in alum (Imject® Alum, Thermo Scientific) into the hind footpads (25ul) ...
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bioRxiv - Cell Biology 2020Quote: ... Equal masses of lysates were separated by SDS-PAGE using either 4-12% Bis Tris gels or 3-8% Tris acetate gels (Thermo Fisher Scientific). All IRS2 immunoblots were separated on 3-8% Tris acetate gels with the exception of those shown in Figures 5A and S4B ...
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A Novel PHOX/CD38/MCOLN1/TFEB Axis Important For Macrophage Activation During Bacterial PhagocytosisbioRxiv - Immunology 2019Quote: ... cells were fixed with 4% paraformaldehyde (PFA) pH 7.4 at room temperature for 10 min and washed 3 times in PBS (Gibco Life Technologies,10010) for 5 min each ...
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bioRxiv - Genetics 2020Quote: ... 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene 3-dodecanoic acid (BODIPY™ FL C12; Thermo Fisher Scientific, USA) (4 µg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... and cells were passaged every 3-4 days once reaching 70-80% confluency using pre-warmed Versene (Fisher Scientific #15-040-066).
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... (3) 65%-95% for 5 min and (4) washout for 15 min) using the EASY-nano LC 1200 system (Thermo Fisher Scientific) with a flow rate of 300 nL/min ...
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bioRxiv - Developmental Biology 2022Quote: ... All other lines were passaged at approximately 80% confluency (every 4-5 days on average) as tiny clusters (3-5 cells on average) using 0.5 mM EDTA (15575020, Gibco, concentration 10 mM).
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bioRxiv - Developmental Biology 2022Quote: ... Epithelial sheets of embryos from the same litter were pooled (3-4 embryos per sample) and incubated for 10 min at RT in TrypLE Select Enzyme (Life Technologies/Gibco). Dissociated cells were resuspended in 0.5% BSA ...
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bioRxiv - Cell Biology 2022Quote: HTM cells cultured atop ECM hydrogels in presence of the different treatments for 3 days were fixed with 4% paraformaldehyde (Thermo Fisher Scientific) at room temperature for 20 min ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed five times with PBS and then twice with distilled water (dH2O) before addition of 5-bromo-4-chloro-3-indolyl-phosphate/NBT (BCIP/NBT) one-step solution (Thermo Fisher Scientific) and incubation at 37°C for approximately 15 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... We used siDESIGN Center (Horizon Discovery) to design siRNA (siUNG#4) targeting the 3′UTR region of UNG mRNA (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2024Quote: HEK-293 cells (ATCC, CRL-1573) were subcultured every 3-4 days in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, 41965-039) supplemented with 10% v/v fetal bovine serum (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in 1x TBS-T with 3% BSA (Fisher Scientific BP1600): α-SARM1 (BioLegend 696602 ...
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bioRxiv - Neuroscience 2023Quote: ... before being spun (5000rpm 4⁰C for 3 minutes) and homogenised (70 seconds with a pestle) in 300 ul trizol (Invitrogen 15596-026). RNA was extracted using the Zymo Direct-zol RNA Microprep Kit (Zymo Research ...
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bioRxiv - Genetics 2023Quote: ... Total RNA was extracted from n = 3–4 biological replicates per sample using the PureLink RNA Mini Kit (Invitrogen, Waltham, MA, USA), and sample quality was evaluated using a TapeStation ...
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bioRxiv - Biochemistry 2022Quote: ... followed by mechanical blotting for 3-4 s and rapid vitrification in liquid ethane with a Vitrobot Mark IV plunge-freezing device (Thermo Fisher Scientific) operated at 4 °C and 100 % relative humidity.
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then centrifuged at 1000rpm for 3 minutes at 4°C before being suspended in 100μl HBSS (ThermoFisher, Cat No. 14025) + 1% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... P8 pups were fed 1 μl 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid BODIPY™ FL C16 (BODIPY FL C16, ThermoFisher Scientific, D3821) diluted in olive oil at a concentration of 10 μg/μL ...
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bioRxiv - Neuroscience 2023Quote: ... Fibroblasts started to migrate out of the skin biopsies after 7-10 days and were transferred to two T75 flasks after 3-4 weeks using the standard splitting protocol with TrypLE™ Express enzyme (Thermo Fisher). Fibroblasts were then cultured in IMDM (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... 7H11 plates contained 50 μg/mL hygromycin and 50 μM 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) (Thermo Scientific). Unless specified ...
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bioRxiv - Pathology 2023Quote: Dried peptide samples were dissolved in 4.8 μL of 0.25% formic acid with 3% (vol/vol) acetonitrile and 4 μL of each sample was injected into an EasynLC 1000 (Thermo Fisher Scientific). Peptides were separated on a 45 cm in-house packed column (360 μm OD×75 μm ID ...
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bioRxiv - Synthetic Biology 2024Quote: ... 100 μM isopropyl-β-D-thiogalactopyranoside (IPTG) and 100 μg/mL 5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside (X-gal) (Thermo Fisher Scientific). After incubation overnight at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Uterine tissues were cut into 3- to 4-mm lengths with sterile scissors and incubated in phenol red-free HBSS(−) containing dispase (#17105-041, Thermo Fisher Scientific) and pancreatin (#P3292 ...
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bioRxiv - Biophysics 2023Quote: ... cells were washed in Ca2+-free PBS and incubated for 20 min at room temperature with 3 μM Fluo-4-AM (F14201, Thermo Fisher Scientific) in PBS while being protected from ambient light ...
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bioRxiv - Cell Biology 2023Quote: ... Samples denatured by 3 min boiling in Laemmli buffer were separated by electrophoresis on 4-12% bis-acrylamide gel (Thermo Fisher Scientific), then transferred to 0.45 µm nitrocellulose membranes (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... for a 35 mm dish 4 μl of Lipofectamine 2000 and selected plasmids (3 μg) were diluted in Opti-MEM medium (Thermo Fisher Scientific) to a final volume of 250 μl and incubated for 20 min at RT ...
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bioRxiv - Biophysics 2023Quote: ... These grids were blotted with filter paper for 3∼6 s (100% humidity at 4 °C) in a Vitrobot Mark IV (Thermo Fisher Scientific) and vitrified in liquid ethane at liquid nitrogen temperature ...
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bioRxiv - Immunology 2024Quote: ... we co-transfected 3×106 3-day activated CD8+T cells with 150 pmol/106 cells of human TSP-4-specific siRNAs (#s14100, Thermo Fisher Scientific) and 1.5 μg/106 of the pMax-TSP-1-GFPSpark construct ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then dipped in the secondary antibody-containing buffer with the nuclear dye 4′,6-diamidino-2-phenylindole (DAPI, Cat# D1306, Life Technologies-Invitrogen, Carlsbad, CA, USA, dilution 1:400), for 2 hours at RT ...
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bioRxiv - Cell Biology 2019Quote: ... The pellet was resuspended 3 times using 7 mL Wheaton Dounce tissue grinder (Fisher Scientific) and centrifuged at 12,000 rpm at 4°C for 30 minutes (Sorvall SS-34 centrifuge rotor) ...
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bioRxiv - Genetics 2020Quote: ... 25 μL of media containing STS and CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) at final treatment concentrations of 1 μM and 2.5 μM ...
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bioRxiv - Neuroscience 2022Quote: ... The electrodes were labelled with DiIC18(7) (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindotricarbocyanine Iodide) (DiR) (Invitrogen) to enable post-mortem reconstruction of the electrode tracks in histological sections.
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bioRxiv - Immunology 2022Quote: ... Alexa Fluor 488 and caspase-3/7 activity detection dyes were purchased from Thermo Fisher, and Viobility™ 405/452 Fixable Dye from Miltenyi Biotec.
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bioRxiv - Microbiology 2023Quote: ... Apoptosis assays were perfomed using CellEvent Caspase 3/7 Green Detection Reagent (Thermo Fisher Scientific) and Live/Dead eFluor 660 (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained with CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: Astrocytes at 3 DIV or at 7-8 DIV after lentivirus infection were loaded with 1 μg/ml Fura-2-AM (#F1221, ThermoFisher) in astrocyte culture medium for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids developed within 7 - 21 days after initial seeding and were first passaged (1:2) by dissociation using TrypLE Express Enzyme (ThermoFisher) and resuspension in OcellO primary organoid medium ...
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bioRxiv - Cancer Biology 2021Quote: ... and 4 μg/ml of blasticidin (ThermoFisher Scientific). HRAS RAS- less MEFs was cultured in 2.5 μg/ml of puromycin (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... for 4 h and DAPI (Thermo Fisher Scientific) staining was used to visualize cytoarchitecture (1:5000 ...
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bioRxiv - Immunology 2021Quote: ... and a Qubit™ 4 Fluorometer (Invitrogen Technologies). Separately labeled cDNA libraries were multiplexed according to the manufacturer’s protocol (Illumina ...
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bioRxiv - Microbiology 2019Quote: ... run on 4-12% Bis-Tris gels (Invitrogen), and transferred to PVDF membranes ...
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bioRxiv - Immunology 2022Quote: ... ELISA plates (Immulon 4 HBX, Thermo Fisher Scientific) were coated with PBS or 2 ug/mL recombinant protein and stored overnight at 4C ...
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bioRxiv - Immunology 2022Quote: ... and quantified using a Qubit 4 Fluorometer (Invitrogen). The length distribution was determined via the TapeStation 4200 (Agilent Technologies ...
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bioRxiv - Immunology 2020Quote: ... di-4-ANEPPDHQ (Thermo Fisher Scientific Cat# D36802). (iii ...
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bioRxiv - Immunology 2019Quote: ... Cells were subsequently fixed in 4% paraformaldehyde (Affymetrix) and permeabilized by incubating for 5 min with PBS containing 0.02% NP-40 ...
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bioRxiv - Bioengineering 2019Quote: ... and 4 µg/ml Gentamicin (1575-060, Gibco). Following recovery for 8-10 days ...
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bioRxiv - Physiology 2020Quote: ... and a Qubit 4 Fluorometer (Thermo Fisher Scientific). RNA libraries were prepared using TruSeq® Stranded mRNA HT Sample Prep Kit (Illumina Inc. ...
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bioRxiv - Plant Biology 2019Quote: Using a Vitrobot Mark 4 (FEI Thermo Fisher), 4 µL of mat3-4 cell culture was blotted onto R2/1 carbon-coated 200-mesh copper EM grids (Quantifoil Micro Tools ...