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Citations for Thermo Fisher :
2501 - 2550 of 10000+ citations for 6 Methyl 2 3 4 9 tetrahydro 1H carbazol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: We diluted freshly purified virus samples in DPBS and combined three volumes of the virus with one volume of NuPAGE LDS Sample Buffer (4×; Thermo Fisher) containing 100 mM dithiothreitol ...
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bioRxiv - Neuroscience 2024Quote: ... were added and incubated for 4 hours at 4°c followed by one wash with lysis buffer (Pierce) with added Halt protease and phosphastase inhibitors (Thermo Scientific) and two washes with PBS and resuspended in PBS for Mass spectrometry.
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bioRxiv - Biochemistry 2020Quote: ... Primary antibodies were removed by 3 washes with 2% BSA 1x PBS and incubated with 2 μg/ml anti-rabbit IgG Alexa Fluor 594 (Thermo Fisher) for 2 h in the dark at 4°C ...
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bioRxiv - Physiology 2023Quote: ... The final plasmids for the sense and antisense expression of daf-2 or aak-2 under the str-3 promoter were generated using Gateway Cloning Technology (Life Technologies) and injected into FLP-7 secretion line (SSR1164 ...
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bioRxiv - Bioengineering 2024Quote: Live and dead cells were stained with 1.6-4 mM calcein AM (C125400; TRC) and 4 mM ethidium homodimer-1 (EthD-1 L3224; ThermoFisher), respectively ...
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bioRxiv - Immunology 2020Quote: ... were stained with 0.25 ug of Mamu MR1 5-OP-RU or Ac-6-FP tetramer for one hour in the presence of 500 nM Dasatinib (Thermo Fisher Scientific; Cat No. NC0897653). When TCRVα7.2 co-staining was performed ...
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bioRxiv - Microbiology 2021Quote: ... were stained with 0.25 ug of Mamu MR1 5-OP-RU or Ac-6-FP tetramer for one hour in the presence of 500 nM Dasatinib (Thermo Fisher Scientific; Cat No. NC0897653). When TCRVα7.2 co-staining was performed ...
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bioRxiv - Genomics 2023Quote: ... the ORF4-6 genomic region was amplified directly from the clinical samples using the SuperScript™ IV One-Step RT-PCR System (Thermo Fisher Scientific, Waltham, MA). Primers were designed using Primer3 (26 ...
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bioRxiv - Molecular Biology 2021Quote: ... LAMP amplification was measured by including 1X NEB LAMP Dye (B1700) or 1 μM SYTO™-9 (ThermoFisher S34854), 0.5 μM SGFdel or 1.0 μM wt beacon ...
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bioRxiv - Genomics 2019Quote: ... BS(PEG)9 was then aspirated and quenched by incubating in 1 M Tris-HCl pH 8.0 (AM9855G, ThermoFisher) for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... and resuspended at a concentration of either 2.5×107 or 5.0×107 cells/mL in 9:1 DPBS:H2O (Gibco 14190-144). Cells were injected into the Duct of Cuvier of 2dpf casper or casper FLK-RFP (labeling the vasculature with RFP ...
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bioRxiv - Systems Biology 2022Quote: ... supplemented with 9% foetal bovine serum (FBS; catalogue number SH3007103; HyClone) and 1% penicillin-streptomycin (catalogue number 15140; Gibco); 293TAT cells were maintained in DMEM (catalogue number 11995 ...
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bioRxiv - Cancer Biology 2020Quote: ... A 2.5 μL aliquot was injected using an Ultimate 3000 (sample height 1 mm, puncture depth 9 mm, ThermoFisher) onto an IonOpticks C18 column (25 cm Aurora Series ...
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bioRxiv - Biophysics 2023Quote: ... Fresh human blood was drawn into a citrated tube (3.8% sodium citrate, citrate:blood = 1:9) and mixed with MitoTracker Deep Red (Invitrogen, USA). Citrate chelates extracellular calcium ...
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bioRxiv - Cell Biology 2022Quote: U2OS (ATCC) cells were cultured in DMEM medium supplemented with 9 % Fetal Bovine Serum and 1 % penicillin/streptomycin (GIBCO). Primary hippocampal neurons were maintained in Neurobasal medium supplemented with 1 % B27 (GIBCO) ...
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bioRxiv - Genomics 2023Quote: ... or mouse anti-YAP1 H-9 (1:150, sc-271134) in a PBS solution containing donkey serum albumin and 0.1% Trizol (ThermoFisher). Cells were washed with PBS (3x for 15mins ...
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bioRxiv - Biochemistry 2024Quote: ... 0.6 μg 3xMyc-Ubiquilin was co-transfected with 5.4 μg pOG44 at a 9:1 ratio of pOG44 to plasmid in Opti-MEM (Gibco) using 3 μL Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: Cultured DRG neurons were loaded with 4 μM Fura-2 AM (Life Technologies) in culture medium at 37°C for at least 60 minutes before use ...
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bioRxiv - Cell Biology 2021Quote: LX-2 and TWNT-4 human HSCs were lysed in TRIzol (Life Technologies) and total RNA was purified following the supplied protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 mL of 2 mM disuccinimidyl glutarate (DSG,Life Technologies, #20593, Carlsbad, CA) prepared in 1X PBS was added per 1 × 107 cells to the conical tube and the solution was mixed thoroughly by pipetting to remove clumps ...
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bioRxiv - Biophysics 2020Quote: ... 2 mM MgCl2) and 4 μM of the phosphobinding protein (PBP) (Life Technologies) to detect the inorganic phosphate (Pi ...
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bioRxiv - Immunology 2020Quote: ... P2X7a 451L or P2X7a 451P were loaded with 2 μM Fluo-4 (Invitrogen) for 20 min at 4°C and 10 min at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... XEN cells were passaged every 2–4 days using TrypLE Express (Thermo Fisher) up to passage 20.
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bioRxiv - Physiology 2023Quote: Freshly isolated cardiomyocytes were loaded with 2 μM Fluo-4-AM (Thermo Fisher) for 20 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... A pCR 4-TOPO or pCR- XL-2-TOPO plasmid (ThermoFisher, Waltham, Massachusetts) with an insert spanning ITS1-5.8S rRNA-ITS2-D1/D2 region of 28S rRNA from a pure culture strain was used as a positive control ...
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bioRxiv - Microbiology 2023Quote: ... and an additional 2 mM L-glutamine (Gibco; 4 mM total L-glutamine). Cells and parasites were incubated at 37°C and 5% CO2 in a humidified incubator ...
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bioRxiv - Cancer Biology 2023Quote: ... Cyclopentylidene-[4-(4ʹ-chlorophenyl)thiazol-2-yl]hydrazone (CPTH2) was purchased from ThermoFisher Scientific and used at a 50µM concentration ...
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bioRxiv - Microbiology 2024Quote: ... then fixed with 2% paraformaldehyde (pH = 7.2-7.4, Fisher Scientific 30525-89-4) for 15m ...
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bioRxiv - Cancer Biology 2021Quote: ... U251 cells were treated with 4 uM CellEvent Caspase-3/7 green detection reagent (Molecular probes) prior to imaging ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was generated using either the Superscript 3 or 4 First Strand Synthesis System (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... pET15b-CDA5 was amplified using primers 3 and 4 and added to the BsiW1 (Thermo Fisher) site of pSLIK using InFusion (Takara ...
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bioRxiv - Biochemistry 2022Quote: To prepare samples a 3:4 dilution was made in NuPAGE LDS sample buffer (ThermoFisher; NP007) supplemented with 10% v/v β-mercaptoethanol ...
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bioRxiv - Cell Biology 2022Quote: ... 10% glycerol) were separated on 3-8% Tris-acetate or 4-12 Bis-Tris gels (Thermofisher). Proteins were then transferred to PVDF membranes (Merck).
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bioRxiv - Microbiology 2020Quote: ... All PBMCs were stimulated for 3-4 days with 2ug/ml of phytohemagglutinin (PHA; Gibco BRL) and 1 ug/ml of interleukin-2 (IL-2 ...
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bioRxiv - Cancer Biology 2022Quote: 500000 cells in suspension were loaded with 3 μM Fluo-4 AM (Thermo Fisher Scientific, F14201) in DMEM without serum supplemented with 0.02% Pluronic-F127 (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: Whole cell lysates were resolved in NuPAGETM 3-8% or 4-12% gradient gels (ThermoFisher Scientific) and transferred onto PVDF membranes using the Trans-blot Turbo transfer system (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... 40 μg/mL 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) (Thermo Scientific) and 1 mM isopropyl beta-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Molecular Biology 2021Quote: Total RNAs for 3 to 4 independent experiments performed in triplicate were extracted by Trizol (Ambion) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... and MCR:SATB2 (45-3 and 63-4) cell lines were cultured in DMEM medium (Life Technologies) supplemented with 10% heat-inactivated FBS (Atlanta Biologicals) ...
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bioRxiv - Genetics 2020Quote: ... Colonies were passaged every 4 – 7 days using 3 – 5min incubation with 0.5mM UltraPure EDTA (Invitrogen) as required.
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bioRxiv - Neuroscience 2022Quote: ... the dissociated DA neurons were incubated with 3 μM Fluo-4 AM (Thermo Fisher, USA, F14201) for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 mL of the homogenized suspension was treated with 3 units/mL Rnase-free Dnase (Ambion) for one hour at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... during a 3-day culture followed by 4 days of incubation with 10% FBS (Invitrogen, USA) as previously described[43] ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were run on NuPage 3-8% or 4-12% Bis-Tris precast gels (ThermoFisher Scientific) and transferred to nitrocellulose ...
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bioRxiv - Genomics 2023Quote: ... at 4°C for 3 hours followed by addition of 10 ml Protein A Dynabeads (Invitrogen) for 20 min to capture the antibody-protein complexes ...
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bioRxiv - Cell Biology 2024Quote: ... and then for 3-4 h with 50 μM Click-iT® HPG (L-Homopropargylglycine) (Invitrogen). For gel electrophoresis analysis ...
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bioRxiv - Cell Biology 2021Quote: ... N-hydroxysulfosuccinimide (sulfo-NHS) and 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) from Thermo Scientific were dissolved in 18 MOhm DDW immediately before use ...
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bioRxiv - Bioengineering 2022Quote: ... 1-ethyl-3-(−3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) was purchased from Fisher Scientific (Pittsburgh, PA, USA). 2-Bromo-2-methylpropionic acid (BMPA) ...
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bioRxiv - Neuroscience 2024Quote: Rabbit monoclonal or polyclonal antibodies include those against 14-3-3 (Invitrogen, 51-0700, 1:1000); clathrin heavy chain (Abcam ...
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bioRxiv - Systems Biology 2020Quote: 1×10^6 cells were seeded in wells of 6-well plates (FALCON # 353046) in MEM without phenol red (Gibco # 51200038) supplemented with 0.5% Fetal Calf Serum (VWR #89510-184) ...