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Citations for Thermo Fisher :
2501 - 2550 of 10000+ citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... An internal standard was prepared from the isotope-labeled standards in 3% acetonitrile with 0.1% formic acid with 5 fmol/µL of Peptide Retention Time Calibration (PRTC) mixture (ThermoFisher Scientific) and 10 µg/mL E ...
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bioRxiv - Bioengineering 2022Quote: ... glacial acetic acid) for 2 hours at room temperature (Section 2.14) or methanol-free 4% formaldehyde (ThermoFisher, Section 2.16) and then kept in PBS at 4°C before washing in gradations of ethanol up to 100% ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... we prepared a 2-fold dilution series of a 1x “stimulation” solution containing 1x essential amino acids (Invitrogen, 11130051), 1x non-essential amino acids (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... were quantified using an avidin and 4’-hydroxyazobenzene-2-carbocylic acid assay according to the manufacturer’s instructions (Fisher Scientific). Briefly ...
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bioRxiv - Physiology 2019Quote: ... Cells were incubated with fura-2AM (2 μM) (Molecular ProbesTM) in SBS containing 0.01% pluronic acid (Thermo Fisher Scientific) for 60 min at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were prepared for analysis by staining with 2 x SYBR Green I nucleic acid gel stain (Life Technologies) for 30 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... diluted 2 times with 0.1 % formic acid and desalted on strong cation exchange micro-tips (StageTips, Thermo Fisher scientific) as described72 ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were resuspended in 400 ul NET-2 and extracted with an equal volume of acid-phenol:chloroform (Invitrogen AM9722). After centrifugation at 16,000 x g for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged twice a week using 2 mM ethylenediaminetetraacetic acid in phosphate buffer saline (EDTA/PBS; Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Fractions were resuspended in 2% acetonitrile and 0.1% formic acid and separated on an Easy nLC 1200 (ThermoFisher Scientific) and a 22 cm long ...
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bioRxiv - Cell Biology 2023Quote: ... by recording the increase in the absorbance at 405 nm after the reaction of reduced glutathione with 5,5’-dithiobis(2-nitrobenzoic acid) (DTNB) for 2.5 min at 15-s intervals using a Varioskan Flash (Thermo Fisher). GSSG was quantified after derivatization of GSH with 2-vinylpyridine ...
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bioRxiv - Bioengineering 2023Quote: ... 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB, aka Ellman’s Reagent) and DL-dithiothreitol (DTT) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2022Quote: ... and L plasmids at a ratio of 4:2:2:2:1 using Lipofectamine 2000 (ThermoFisher Scientific). Cells were transfected in 6 well plates and subsequently transferred to T25 flasks with HEp2 cells until cytopathic effect (CPE ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μM Rhod-2 AM (Thermo, R1244) and 2 μM Calcium green-1 AM (Life Technologies, C3011MP) was added to culture media and allowed to stain at 22 °C for 20 min prior to imaging (frozen Rhod-2 AM aliquots were used only when the DMSO suspension remained clear) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Developmental Biology 2019Quote: ... pH=6 (ThermoFisher). Sections were then blocked for 1h at RT with 10% normal donkey serum (Chemicon International Inc ...
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bioRxiv - Biochemistry 2024Quote: ... or 6% (Thermofisher) tris-glycine SDS-PAGE gels then transferred onto PVDF membranes using the Transblot rapid transfer machine (BioRad) ...
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bioRxiv - Neuroscience 2021Quote: ... 1:6 and 1:9 or 1:10 and 1:100) of ISF samples were prepared using OPTIMEM+ Glutamax-1 (Gibco #51985-026) and kept on ice until lipofection ...
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bioRxiv - Developmental Biology 2024Quote: ... Feeder-depleted serum cultured ESCs were plated on gelatin-coated plates at 10,000 cells/cm2 (90,000 cells per well in a 6-well plate) in advanced N2B27 defined media composed of a 1:1 mix of 1:1 DMEM/F12 (Invitrogen, 12634-010) and Neurobasal medium (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... RD18 and JR1 cells were cultured for 2-5 days in DMEM supplemented with 2% horse serum (Gibco, Carlsbad, CA, USA) at 90-95% confluency ...
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bioRxiv - Evolutionary Biology 2022Quote: ... pseudonana expressing VHAB-eGFP were incubated with the acidotropic pH stain 2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy)phenyl)oxazole (PDMPO) (LysoSensor YellowBlue DND-160; Life Technologies) at a final concentration of 0.125 μM without washing in F/2 ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Peptide mixtures were separated at a 400 nl/min flow rate using a 35 min ACN gradient in 0.5% acetic acid (5–38% ACN gradient for 23 min followed by 38-60% ACN gradient for 3 min and 60-95% ACN gradient for 2 min plus another 3 min at 95% CAN prior to 95%-5% gradient for 2 min and another 2 min at 5% ACN) and detected on an Q Exactive HF mass spectrometer (Thermo Scientific). Dynamic exclusion was enabled for 20 sec and singly charged species ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted from ∼2-5 mg of dechorionated 0–2 hr embryos per biological replicate using TRIzol Reagent (#15596026, ThermoFisher Scientific) and treated with1 μL TURBO Dnase (2 U/μL ...
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bioRxiv - Cancer Biology 2024Quote: ... and washed four times with PBS (2 x quick, 2 x 5 min) prior to incubating with streptavidin conjugated to AlexaFluor647 (Thermo Fisher) diluted in high-salt buffer (0.5 M NaCl ...
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bioRxiv - Cancer Biology 2024Quote: ... Organoid proliferation used the thymidine analogue 5-ethynyl-2’-deoxyuridine (EdU) for 2 hrs using Click-iT EdU Alexa Fluor-555 Imaging Kit (Invitrogen, C10338). H&E and IHC slides were viewed by brightfield-fluorescent microscopy (Olympus BX60) ...
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bioRxiv - Biophysics 2019Quote: ... Cells were transfected with 1:1 mEmerald-Zyxin-6 to mCherry-Paxillin-22 using Lipofectamine 3000 (ThermoFisher) per the manufacturer’s protocol and allowed to grow for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... the medium was replaced with a 1:1 mix of Essential 6 Medium (E6) (Thermo Fisher Scientific) and E8 medium ...
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bioRxiv - Bioengineering 2019Quote: ... native gels were run with 5 μL per well of a 1:5:1 mixture of sample:water:BlueJuice loading dye (Thermo Fisher Scientific) in a 10% TBE ...
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bioRxiv - Microbiology 2022Quote: ... The infected cells were resuspended in HL5c containing 5 U mL−1 of penicillin and 5 μg mL−1 of streptomycin (Invitrogen) to prevent extracellular bacterial growth ...
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bioRxiv - Genomics 2021Quote: ... these droplets were transferred at a density of 6 droplets per 1 well of a 6-well plate into STEP3 medium containing a 1:1 mixture of DMEM/F12 (Invitrogen) and Neurobasal with 0.5X N2 supplement (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... Ten-fold serial dilutions (1×10−1 to 1×10−6) of virus stocks were prepared in MEM (supplemented with 25 mM HEPES (Gibco), 2mM L-Glutamine (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Interleukin 6 (IL-6) was measured using an IL-6 ELISA kit following manufacturer’s instruction (Invitrogen, Catalog # CHC1263). Similarly ...
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bioRxiv - Developmental Biology 2021Quote: ... 6-well plate in mTeSR media with 1:200 P/S (Gibco, 15140122) and 1:1000 Y-27632 (STEMCELL ...
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bioRxiv - Cell Biology 2020Quote: ... live embryos in 6-well plates were incubated in YO-PRO-1 (Invitrogen) staining solution (2 μM YO-PRO-1 in E3 medium ...
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bioRxiv - Cell Biology 2022Quote: ... 1:6 pre-absorbed mouse α-V5 primary antibody (R960-25, Thermo Fisher) was diluted to 1:800 in 1X PBS-BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg mCherry-LacR-RIF1 constructs and 6 μL Lipofectamine 2000 (Thermo Scientific) were incubated in Opti-MEM (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... 6 µg RNA was used and RNaseOUT recombinant ribonuclease inhibitor (1 µl) (Invitrogen) was added ...
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bioRxiv - Immunology 2023Quote: ... 1 mg/ml Collagenase IV and 6 U/ml DNase I (ThermoFisher Scientific) at 37°C and 750rpm for 30 minutes21,22 ...
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bioRxiv - Neuroscience 2023Quote: ... Hepa 1-6 (Murine hepatoma) cells were grown in DMEM (Gibco; 11995-065) supplemented with 10% heat-inactivated FBS (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... Colonies were stained by incubating with 6% Glutaraldehyde (#BP2547-1, Thermo Fisher Scientific) for 20 minutes and 0.05% w/v crystal violet (#C581-25 ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-paxillin (Invitrogen, Cat. AHO0492, 1:5), mouse-anti-NCAM1 (DSHB ...
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bioRxiv - Microbiology 2021Quote: ... 5 mg ml-1 proteinase K (Thermofisher Scientific). DNA was isolated with phenol-chloroform extraction ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl of dithiothreitol (DTT, 5 mM; Invitrogen), 1 μl of RNase-OUT (40 U ...
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bioRxiv - Genomics 2021Quote: ... 5 μL Streptavidin C-1 beads (Thermo Fisher) were Washed with Tween Wash Buffer (5 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... anti-claudin-5 (Invitrogen, USA, 1:1000 dilution), anti-p-ERK1/2 (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... β-Tubulin (1:5 000, MA5-16308, Thermo Fisher) PINK1 (1:1 000 ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were washed three times in PBS and incubated in secondary antibody (2 mg/mL Alexa Fluor 488, Invitrogen, 1:1500, 2 mg/mL Alexa Fluor 594, Invitrogen, 1:1500; 2 mg/mL Alexa Fluor 568, Invitrogen, 1:1000) diluted in PBS for 1.5 h at room temperature ...