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Citations for Thermo Fisher :
2451 - 2500 of 5300 citations for Octreotide acetate impurity H since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 18 or 24 h of incubation cells were washed three times with 500uL of PBS (Gibco) to eliminate non-adherent bacteria ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was reverse transcribed with the Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Scientific). RTqPCR was performed using the PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used for Western blotting: 680 Donkey anti rabbit (H+L) (1:20,000, Thermo Fisher Scientific Cat# A10043 ...
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bioRxiv - Neuroscience 2024Quote: ... sections received three 1-h rinses and were coverslipped using ProLong Diamond antifade mountant (ThermoFisher Scientific).
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bioRxiv - Plant Biology 2024Quote: Maxima H Minus First Strand cDNA Synthesis Kit (200 U) (ThermoFisher Scientific, Waltham, MA, USA; K1652)
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bioRxiv - Genomics 2024Quote: ... Total RNA extracts were submitted to cDNA synthesis with SuperScript III RNase H-reverse transcriptase (ThermoFisher) and the resulting cDNA was used as a template in real-time PCR assays.
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bioRxiv - Genomics 2024Quote: ... consisting of 1 uL of 200 U/uL Maxima H Minus Reverse Transcriptase (EP0752, Thermo Scientific), 1 uL of 5 U/uL ThermaStop-RT reagent (TSTOPRT ...
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bioRxiv - Biophysics 2024Quote: ... and goat anti-rabbit IgG (H+L) AlexaFluor488 (1:400, 10729174, Fisher Scientific GmbH,Schwerte, Germany) for 2 hrs at room temperature ...
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bioRxiv - Systems Biology 2024Quote: ... cDNA synthesis was performed with RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, K1632), with the following components ...
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bioRxiv - Cell Biology 2024Quote: ... 72 h later the cell nuclei were stained with 2 µM Hoechst (62249, Thermo Fisher Scientific) in PBS and the cells were counted using the Celigo image cytometer.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were transfected after 48 h using Lipofectamine RNAimax (Life Technologies, USA, Cat No. 13778-150) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... was washed by soaking it for 16 h in diethylpyrocarbonate (DEPC)-treated water (Thermo Fisher Scientific) on a Fisherbrand Multiplatform Shaker (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: One microgram of RNA was reverse transcribed using Revert Aid H Minus Reverse Transcriptase (Thermo Scientific), and the RT product was used for expression analysis using Maxima SYBR Green/ROX qPCR Master Mix (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Gels were washed and stained for 1 h with Simply Blue™ Safe Stain (Life Technologies). After washing for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... goat anti-rabbit (H+L) secondary HRP conjugate was purchased from Invitrogen (#31460, WB 1:5000), Actinomycin D was purchased from Sigma Aldrich (#A1410-2MG).
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bioRxiv - Developmental Biology 2024Quote: ... Tadpoles were blocked for 1 h at room temperature in 10% CAS-block (Invitrogen #00-8120) in PBT ...
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bioRxiv - Developmental Biology 2024Quote: ... and Goat anti-Mouse IgG (H+L) Secondary Antibody-HRP (Thermo Fisher Scientific, 31430, 1:2000). Protein bands were detected with ECL kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... slides were incubated with secondary goat anti-rat IgG (H+L) Alexa Fluor 488 (1:500; Invitrogen) and goat anti-mouse IgG (H+L ...
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bioRxiv - Neuroscience 2021Quote: ... Autophagy was activated by keeping cells in HBSS for 2 h prior to treatment (24020-091, Invitrogen) and blocked by addition of 3-MA (Sigma- Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... for immunofluorescence - a goat anti-mouse IgG (H+L) secondary antibody Alexa Fluor 555 (#A31572, Invitrogen, USA), a goat anti-rabbit IgG (H+L ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA product was then reverse transcribed to cDNA using Maxima H Minus Reverse Transcriptase (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were allowed to grow for 24 h post seeding before transfection with Lipofectamine® 2000 (Invitrogen) with 1.6 μg plasmid DNA according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were donkey anti-goat IgG (H+L) Alexa Fluor 680 (1:10,000, Thermofisher, A-21804) and goat anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... Donkey antimouse IgG (H+L) Alexa Fluor 555 (Thermo Fisher; Cat# A-31570; used at 1:250), Donkey Anti-rat IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was obtained using a RevertAid H Minus First Strand cDNA Synthesis Kit with random hexamers (ThermoFisher). qPCR experiments were performed with a CFX Connect thermal cycler (Bio Rad ...
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bioRxiv - Cell Biology 2020Quote: ... 1st strand cDNA was generated using Maxima H minus First Strand cDNA Synthesis Kit (Thermo Scientific, K1652) with the addition of 1ug of Actinomycin D (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... The reaction was terminated at 70°C for 15 minutes then 1 μl of RNase H (Invitrogen) was added to each reaction and incubated at 37°C for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Ribosome-protected fragments (RPF) were generated by 1 h of digestion with 15 U RNase I (Ambion)/OD260nm at 25°C) ...
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bioRxiv - Developmental Biology 2021Quote: ... goat anti-guinea pig IgG (H + L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor 555 (Invitrogen #A21435) at 1:500 ...
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bioRxiv - Developmental Biology 2021Quote: ... and goat anti-mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor 488 (Invitrogen #A32723) at 1:500 ...
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bioRxiv - Developmental Biology 2021Quote: ... were used and developed using anti-goat IgG (H+L)-HRP conjugated antibodies (1:500 dilution, Invitrogen).
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bioRxiv - Developmental Biology 2021Quote: ... full length cDNA was synthesized using Maxima H-reverse transcriptase (K1651, Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s protocol with 2 μg of total HH37 chick ...
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bioRxiv - Developmental Biology 2020Quote: ... DNase-treated and 1µg used for cDNA synthesis with RevertAid H-Minus reverse transcriptase (Thermo Scientific EP0451). Quantitative (q)PCR was performed using SYBR Green Jump Start Taq Ready Mix (Sigma S4438 ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies were Alexa Fluor 488 goat anti-rabbit IgG (H + L) (Thermo Fisher Scientific, 1:200), Alexa Fluor 547 goat anti-mouse IgG (H + L ...
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bioRxiv - Cell Biology 2022Quote: ... and separated at 120 V for ~1 h in BupH Tris-HEPES-SDS running buffer (ThermoFisher Scientific). The proteins were transferred to a nitrocellulose membrane (GE Water & Process Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... and run at 170 V for 1 h in NuPAGETM MOPS SDS Running Buffer (Thermo Fisher Scientific). Proteins were transferred to a 0.2 µm nitrocellulose blotting membrane (GE Healthcare ...
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bioRxiv - Bioengineering 2022Quote: ... cDNA was synthesized using 100-200 ng RNA with the Maxima H minus RT (Thermo Fisher, #EP0751) according to the manufacturer protocol and using random hexamers for priming ...
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bioRxiv - Molecular Biology 2021Quote: ... The ligated products were subjected to rRNA depletion with complementary DNA oligomers (IDT) and RNase H (Invitrogen)138 ...
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bioRxiv - Molecular Biology 2020Quote: ... The secondary antibodies were HRP-conjugated goat anti-mouse IgG (H+L) (1:10,000; 31430, Thermo Fisher) and anti-rabbit IgG (1:10,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... The transfection mixtures were replaced at 6 h post-transfection by 293 SFM II expression medium (Gibco), supplemented with sodium bicarbonate (3.7 g/L) ...
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bioRxiv - Molecular Biology 2021Quote: 6 μg total oocyte RNA from pooled injected oocytes was treated with 10 U RNase H (Ambion) and 500 ng oligo(dT ...
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bioRxiv - Molecular Biology 2020Quote: ... The transfection mixtures were replaced at 6 h post-transfection by 293 SFM II expression medium (Gibco), supplemented with sodium bicarbonate (3.7 g/L) ...
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bioRxiv - Cell Biology 2022Quote: ... followed by a 1 h-incubation with 100 μL protein A/G agarose beads (Thermo Scientific Pierce) at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Non-adherent cells were washed away after 1.5 h and the remaining cells incubated in RPMI (Gibco) supplemented with 10 % heat-inactivated pooled human serum (Sigma ...
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bioRxiv - Genomics 2020Quote: ... 3.7 U/μl DNA polymerase I and 0.2 U/μl Ribonuclease H (all from ThermoFisher Scientific, USA)) for 2h at 16°C ...
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bioRxiv - Immunology 2021Quote: ... The secondary anti-body was anti-Mouse IgG (H+L) Alexa Fluor 488 (Invitrogen; dilution 1:1000), Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 h nocodazole treated cells were washed with PBS and assayed for toxicity using AlamarBlue (ThermoFisher). Used at a 1x dilution ...
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bioRxiv - Biochemistry 2020Quote: Cells were plated at ~30% confluency and transfected 24 h later using the Lipofectamine 3000 system (Invitrogen) with a mixture containing 1 μg of retroviral vector with the construct of interest ...
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bioRxiv - Genetics 2020Quote: ... and Alexa Fluor 568-conjugated goat anti-rabbit IgG (H+L) secondary antibodies (1:200; Thermo Fisher) at 4°C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were then washed with PBT for 2 h and stored in 2 % DABCO (Acros Organics, AC112471000).