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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for Estriol ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... and mRNA was synthesized using an in vitro RNA transcription kit (mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit, AM1345, Invitrogen). sgRNAs were amplified and transcribed in vitro using the MEGAshortscript™ T7 Transcription Kit (AM1354 ...
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bioRxiv - Cell Biology 2020Quote: ... NotI-linearized plasmids were purified (QIAquick Gel Extraction Kit) and the 5’-capped mRNAs were synthesized using SP6 mMessenger mMachine kit (Ambion). For GFP-centrin ...
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bioRxiv - Neuroscience 2021Quote: Cell viability was determined using a commercially available dual-wavelength fluorescent kit (LIVE/DEAD Viability/Cytotoxicity Assay Kit; L3224; Invitrogen). Live cells are detected by intracellular esterase activity using the dye calcein-AM (∼495/515 nm ex/em ...
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bioRxiv - Systems Biology 2021Quote: ... DNA was extracted from 1 mL of this outgrown culture using a GeneJet Genomic DNA Purification Kit (ThermoScientific) and the concentration of genomic DNA was quantified using a Qubit dsDNA HS Assay Kit (Invitrogen). A 1 μg aliquot of genomic DNA was suspended in 15 μL of 10 mM Tris buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... sgRNAs were transcribed with the MEGAshortscript T7 Kit from synthetic DNA oligomers and purified with the MEGAclear Transcription Clean-Up Kit (both ThermoFisher).
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bioRxiv - Microbiology 2020Quote: ... nuclear soluble and chromatin-bound protein fractions were extracted from cells using the Subcellular Protein Fractionation Kit for Cultured Cells kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were purified using the GeneJet Plasmid Miniprep kit (Thermo K0503) or the Purelink HiPure Plasmid Midiprep kit (Invitrogen K210005). Site-directed mutagenesis of plasmids was performed by DNA synthesis with 2.5 U PfuUltra II Fusion HS DNA Polymerase (Agilent) ...
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bioRxiv - Immunology 2020Quote: ... were transfected with the plasmid expressing S-2P protein ectodomains by ExpiFectamine 293 transfection kit and ExpiFectamine CHO transfection kit (ThermoFisher), respectively ...
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bioRxiv - Developmental Biology 2021Quote: ... Each plasmid was linearized with NotI restriction enzyme and mRNAs were synthesized by in vitro transcription with 1 µL of GTP from the kit added to the mix and lithium chloride precipitation (using the mMESSAGE mMACHINE sp6 Ultra kit #AM1340, Ambion).
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bioRxiv - Genomics 2021Quote: ... total RNA was extracted from DIII pearlfish embryos with a Qiagen RNeasy mini plus kit and reverse transcribed with a superscript IV VILO kit from ThermoFisher according to manufacturers’ instructions ...
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bioRxiv - Microbiology 2021Quote: RT-PCR was performed using SuperScript™ III One-Step qRT-PCR System kit (commercial kit #1732-020, Life Technologies) with primers ORF1ab_Fw ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from pooled 7-day old seedlings using the Qiagen RNeasy Plant Mini Kit and complementary DNA was synthesized using the Maxima cDNA Synthesis kit (Thermofisher). Quantitative RT-PCR analysis was performed on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... Total protein in brain tissue was determined using a protein assay kit (Pierce™ BCA Protein Assay Kit; ThermoFisher, 23225) following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... and nuclear compartments using consecutive centrifugation steps of varying speeds and specialized buffers obtained from a commercially available kit (Sub-Cellular Protein Fractionation Kit for Tissues, ThermoFisher). Bradford protein assays were performed for each compartment individually ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was removed by the DNase treatment kit (DNA-free™ DNA Removal Kit, Invitrogen, Thermo Fisher Scientific, Massachusetts, USA). Parasites were synchronized by 5% sorbitol (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... ABE8e or ABE9 mRNA was transcribed using the in vitro RNA transcription kit (mMESSAGE mMACHINE® T7 Ultra Kit, Ambion). mRNA was purified with MEGAclear™ Kit (Ambion) ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were measured using the BCA Protein Assay Kit (Pierce™ BCA Protein Assay kit, Thermo Fisher Scientific, 23225) and a Tecan Infinite M200 Plate Reader ...
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bioRxiv - Molecular Biology 2024Quote: ... The ratio of DNA concentrations as measured with Qubit dsDNA assay kit and Qubit ssDNA assay kit (Thermo Fisher Scientific) was assessed ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products of bisulfite-treated DNA were purified with a Gel Extraction kit (NZY Tech) and cloned into TOPO cloning kit (Thermofisher). Individual clones were sequenced by standard Sanger sequencing and data were analysed by the online tool QUMA 54.
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bioRxiv - Microbiology 2024Quote: ... the PCR products were purified using the E-Z 96 Cycle Pure Kit (Omega) and quantified with Qubit dsDNA HS Assay Kits (Invitrogen). The purified PCR products were then pooled equally for subsequent sequencing ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were digested using PreOmics sample kit (iST Kit, preomics) and analyzed in Q ExativePlus mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2024Quote: ... and cDNA was synthesized with a commercially available kit (High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor, Thermo Fisher).
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bioRxiv - Molecular Biology 2023Quote: mRNA was prepared with a Qiagen RNeasy kit and cDNA synthesis was performed with a SuperScript VILO kit (Life Technologies), per the manufacturers’ instructions ...
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bioRxiv - Immunology 2023Quote: ... 0.1-1.0μg of RNA was converted to cDNA using the iScript cDNA synthesis kit (Bio Rad) or the High-Capacity RNA-to-cDNA kit (ThermoFisher). qPCR was done using either SsoFast EvaGreen supermix and a BioRad CFX96 Real Time System (Vancouver) ...
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bioRxiv - Immunology 2023Quote: ... Total protein quantification was determined using Pierce™ BCA Protein Assay Kit (Thermo Scientific Pierce™ BCA Protein Assay Kit) and samples were stored at -80°C until use.
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bioRxiv - Neuroscience 2023Quote: ... and the cRNA was generated from each plasmid by using an RNA preparation kit (mMessage mMachine T7 Transcription Kit; Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the appropriate band was purified (ZymoClean Gel DNA Recovery Kit, ZymoResearch) before quantification by fluorescence (Qubit dsDNA HS Kit, Invitrogen).9
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bioRxiv - Molecular Biology 2023Quote: The TaqMan assays were performed using a one-step TaqMan probe kit (TaqManTM RNA-to CTTM 1-Step Kit, Invitrogen). As the TaqMan probe we used 12➔7 Probe FAM–ACCATCAGCCCCCTTTTTATTTCCT-MGBNFQ ...
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bioRxiv - Neuroscience 2023Quote: ... Lumbar spinal cords were dissected in oxygenated artificial cerebrospinal fluid and immediately homogenized using Cytoplasmic Extraction Reagent Kit from the NE-PERTM nuclear extraction kit (ThermoFisher) with a protease inhibitor cocktail added (5mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... after which the entire pool was purified using the Qiagen MinElute purification kit and quantified via Qubit dsDNA HS assay kit (Invitrogen) and sequenced at Novogene on 1 HiSeq PE 150 lane (6 bp ...
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bioRxiv - Microbiology 2023Quote: The protein content of pEVs were quantified using Pierce BCA kit according to the manufacturer’s instructions (BCA Protein Assay Kit, Thermo Fisher).
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was purified using a Qiagen PCR purification kit and then phosphorylated using an Anza PNK kit (Invitrogen). Phosphorylation reactions were inactivated by incubating at 80°C for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... NotI - linearized plasmids were purified (QIAquick Gel Extraction Kit) and the 5’-capped mRNAs were synthesized using SP6 mMessenger mMachine kit (Ambion). For mRNA injection ...
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bioRxiv - Cell Biology 2024Quote: ... EZNA total RNA kit (Omega) was used for RNA isolation and High-Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific) was used for cDNA synthesis by following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: RNA-FISH combined with immunocytochemistry assays for TMEM9B-AS1 and Desmin were performed using the ViewRNA Cell Plus Assay Kit Assay Kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... and StemPro™ Chondrogenesis Differentiation kit (Thermo Fisher) added to the cell pellet ...
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bioRxiv - Genetics 2021Quote: ... Pierce™ BCA protein assay kit (Thermo Fisher) was used to quantify the protein levels in the supernatant ...
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... and visualized by ECL kit (Thermo Fisher Scientific) or WesternBright Sirius kits (Advansta ...
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bioRxiv - Cancer Biology 2021Quote: ... Coomassie Protein Assay Reagent kit (Thermo Fisher Scientific) was used to measure protein concentration ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized using Superscript IV kit (Invitrogen) using random primers ...
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A lateral protrusion latticework connects neuroepithelial cells and is regulated during neurogenesisbioRxiv - Developmental Biology 2021Quote: ... The pENTR/D-TOPO cloning kit (ThermoFisher, K240020) was used to introduce the PCR products into the entry vector for Gateway cloning ...
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bioRxiv - Cell Biology 2019Quote: mRNA harvested using Dynabeads mRNA Direct kit (Invitrogen) was converted to cDNA using qPCRBIO cDNA Synthesis kit (#PB30.11-10 ...
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bioRxiv - Cell Biology 2020Quote: ... High Capacity cDNA Reverse Transcription kit (Life Technologies) was used to transcribe RNA to cDNA using High Capacity RNA–to-cDNA Kit (Applied Biosystems Foster City ...
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bioRxiv - Cell Biology 2020Quote: ... The ATP Determination Kit (Invitrogen, Carlsbad, CA, USA) was used following normalization of total protein concentration according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... All differentiation kits were purchased from Life Technologies. After inducing differentiation for 25 days ...
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bioRxiv - Cell Biology 2020Quote: ... DNA purification kit (Thermo fisher scientific, cat # K0512) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2020Quote: ... The GeneChip 3’ IVT PLUS Reagent Kit (Affymetrix/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... and CDP-Star chemiluminescence kit (Invitrogen, cat.#T2307). Signals were quantified relative to Pgk1 or Tubulin (Fig EV1C ...
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Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosomebioRxiv - Cell Biology 2020Quote: ... Reactions were cleaned using the MEGAclear kit (Invitrogen), and the 50 μL T3 and T7 reactions were mixed with 50 μL of 3x soaking buffer (32.7 mM Na2HPO4 ...
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bioRxiv - Genetics 2021Quote: ... or the MEGAscript T7 Transcription kit (Thermo Fisher), and purified using the RNA Clean & Concentrator kit (Zymo) ...