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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: 6 μg total oocyte RNA from pooled injected oocytes was treated with 10 U RNase H (Ambion) and 500 ng oligo(dT ...
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bioRxiv - Molecular Biology 2021Quote: ... All qPCR runs were performed using QuantStudio-6/ ViiA7/ 7900HT Fast Real-Time PCR System (Applied Biosystems) on 96-well or 384-well plates ...
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bioRxiv - Molecular Biology 2020Quote: ... The transfection mixtures were replaced at 6 h post-transfection by 293 SFM II expression medium (Gibco), supplemented with sodium bicarbonate (3.7 g/L) ...
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Prom1 and Notch regulate ciliary length and dynamics in multiciliated cells of the airway epitheliumbioRxiv - Cell Biology 2022Quote: ... 4′,6-diamidino-2-phenylindole (DAPI) was used to stain intracellular DNA (NucBlue, Thermo Fisher, Waltham, MA). Alexa Fluor 647 Phalloidin (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: ... Sections were stained with DAPI (4′,6′-diamidino-2-phenylindole) and mounted in Prolong Gold (Life Technologies). Images were obtained by confocal microscopy (Nikon C2+ Eclipse ...
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bioRxiv - Genetics 2019Quote: Genotype information was available for 21,001 NTR participants from 6 different genotyping arrays (Affymetrix 6.0 [N = 8,640], Perlegen-Affymetrix [N = 1,238] ...
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bioRxiv - Biophysics 2019Quote: ... 106 cells plated per well of a 6-well plate were transfected with 150 pmol siRNAs (Ambion: Negative Control #AM4611 ...
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bioRxiv - Biophysics 2019Quote: Primary mouse cortical neurons (MCN) from C57BL/6 embryonic day-17 mice were obtained from Thermo Fisher Scientific (cat ...
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bioRxiv - Biophysics 2019Quote: ... and spun at 31,920 g for 10 minutes in a Sorvall RC 6+ (Thermo Scientific, Waltham MA) centrifuge using an F18-12×50 rotor (Thermo Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue was washed in PBS before counterstaining with 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) and mounting ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... 6 μg of mRNA was reverse-transcribed with RevertAid First-Strand cDNA Synthesis Kit (Thermo Scientific, USA) using a thermal cycler (T100TM Thermal Cycler ...
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bioRxiv - Cell Biology 2019Quote: Laurdan (6-dodecanoyl-2-dimethylaminonaphthalene; product D250) and di-4-ANEPPDHQ (product D36802) were purchased from ThermoFisher. Methyl-β-cyclodextrin (product C4555 ...
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bioRxiv - Biochemistry 2019Quote: ... Plates were sealed using optically clear films and transferred to a QuantStudio 6 qPCR instrument (Applied Biosystems). The fluorescence intensity was measured during a temperature gradient from 25 to 95 °C at a constant rate of 0.05 °C/s ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA from whole retinas was extracted using TRIzol (6 retinas from 3 fish per sample) (Invitrogen). RNA was quantified using Nanodrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: All samples were counterstained with DAPI (4’,6-diamidino-2-phenylindole; Invitrogen, Fisher Scientific AG, Reinach, Switzerland) to visualize nuclei ...
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bioRxiv - Genetics 2019Quote: ... SYBR green real-time PCR cycling conditions using QuantStudio 6 Flex Real-Time PCR system (ThermoFisher Scientific) and amplification efficiency calculation (E=e(−1/slope) ...
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bioRxiv - Cell Biology 2021Quote: ... then stained for one minute with 30 ng/mL 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen D1306) in 1x PHEM ...
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bioRxiv - Neuroscience 2020Quote: ... the slides were incubated in 1μg/ml of 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) at RT for 10min ...
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bioRxiv - Cancer Biology 2020Quote: ... HCC1937 cells were seeded in a 6 well plate and treated with CYC065 (Cyclacel) or CVT313 (Thermofisher) at the calculated IC5 ...
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bioRxiv - Cell Biology 2021Quote: ... The digested peptides were labelled with 0.2 mg per reaction of 6-plex TMT reagents (ThermoFisher scientific) (126 ...
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bioRxiv - Cell Biology 2021Quote: ... three times for 6 minutes and prepared for mounting using Invitrogen Slow Fade mounting medium (Invitrogen S36917). The cover glass holding the sample (hydrogel plus cells ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were mounted with ProLong Diamond Antifade Mountant with 4′,6-diamidino-2-phenylindole (DAPI; Life Technologies) and imaged using a Leica SP5 DMI (zebrafish sections ...
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bioRxiv - Cell Biology 2021Quote: ... 4′,6-diamidino-2-phenylindole (DAPI) (cat#D-1306) and TRIzolTM (cat#15596018) were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Systems Biology 2020Quote: ... PCR amplification was performed with a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific) in 20-μl reactions using 1 μl of cDNA (10 ng of total input RNA) ...
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bioRxiv - Neuroscience 2020Quote: ... The nuclei were stained with 300 nM DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific, Slovakia) for 1 min ...
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bioRxiv - Microbiology 2021Quote: ... Reverse 5’-CGA AGG TGT GAC TTC CATG-3’) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). A standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
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bioRxiv - Physiology 2021Quote: ... IL-6 (assay ID: HS00985639_m1) and TNF-α: (assay ID: HS00174128_m1) following manufacture’s protocol (Thermo Fisher Scientific). 18s (assay ID ...
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bioRxiv - Microbiology 2021Quote: ... Desalted peptides were labeled with TMT 6-plex reagents (TMT6plex™ Isobaric Label Reagent Set, Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative real-time PCR was performed on a real-time PCR system (QuantStudio 6 Flex, Applied Biosystems) with Power SYBR Green RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2020Quote: ... DNA was degraded by adding 6 μl of micrococcal nuclease (0.5 U/ml stock, Thermo Scientific, FEREN0181) and 12 μl of calcium chloride (100 mM stock ...
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bioRxiv - Microbiology 2021Quote: Quantitative polymerase chain reaction (qPCR) was completed on a QuantStudio™ 6 Flex System (Applied Biosystems™) with samples prepared on a 384-well (0.2 mL ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR was performed on a real-time PCR system (QuantStudio 6 Flex, Applied Biosystems) with SYBR Green PCR Master Mix (Life Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... 5 to 6 seeds were pipetted into 120 x 120 mm square petri dishes (Thermo Fisher Scientific) containing half-strength Murashige and Skoog salts (MS) ...
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bioRxiv - Neuroscience 2021Quote: ... or pEGFP-N1-TG2 vector (Furini et al., 2018) using 6 μl of Lipofectamine2000 (Thermo Fisher Scientific) in a total volume of 100 μl per slide (about 1.7×105 neurons) ...
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bioRxiv - Cancer Biology 2020Quote: YUMM1.7 and YUMMER 1.7 cell lines were plated in 6-well dishes and cultured in PBS (Gibco), 1ng/mL ...
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A Polybasic Domain in aPKC Mediates Par6-Dependent Control of Membrane Targeting and Kinase ActivitybioRxiv - Cell Biology 2020Quote: ... and Par-6::Hisx6 were expressed in Expi293F cells via transfection using the ExpiFectamine Transfection Kit (Gibco). Approximately 22.5×107 suspension-adapted Expi293F cells were diluted in Expi293 Expression Medium to the final volume of 76.5 ml and cultured in a 37°C shaker (Eppendorf New Brunswick S41i) ...
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bioRxiv - Cancer Biology 2021Quote: MC-38 and CT26.WT (1.5 x 105 cells/well) were seeded in 6-well plates (Nunc) and allowed to attach overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Cover glasses were mounted on slides with mountant with 4’,6-diamidino-2-phenylindole (DAPI) (Life Technologies) and imaged with a confocal microscope.
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bioRxiv - Cancer Biology 2022Quote: ... and HIEC-6 cells were seeded onto 8-well Lab-Tek glass chamber slides (Nunc, Rochester, NY) at 10,000 cells/well ...
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bioRxiv - Bioengineering 2022Quote: ... cells were stained with antibodies and 500ng/mL 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI, Invitrogen # D1306). Samples were analyzed on an LSR II flow cytometer (BD).
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bioRxiv - Cell Biology 2022Quote: ... The samples were sectioned at the thickness of 6 μm using the HM525 NX Cryostat (Thermo Scientific) and stored at −20 °C prior to analysis ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Tandem Mass Tag™ 6-plex (TMTsixplex™) reagents kits for isotopic labeling were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biochemistry 2022Quote: ... Real-time PCR was carried out on the QuantStudio 6-flex Real-time PCR System (ThermoFisher Scientific) using SYBR Green FastMix (QuantaBio ...
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bioRxiv - Microbiology 2022Quote: ... Mel Juso cells stably expressing the Tet Repressor from the pcDNA™ 6/TR vector (Life Technologies) were made following the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: RNA from Flox and iKO fibroblasts grown in 6-well plates was isolated using TRIzol reagent (Ambion). Following chloroform extraction ...
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bioRxiv - Immunology 2022Quote: HeLa-CIITA cells were incubated in 6-well plates using 2-4.105 cells/well using OPTIMEM (Gibco) complemented with 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa M2 cells were seeded in a 6-well plate (Fisher Scientific, product number 50-202-137) on the day before transfection and were transfected with 1 μg of plasmid DNA per well using FuGENE-HD reagent (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... 6 or 9 μl of 20 μM siRNA and 4 μl of lipofectamine RNAi MAX (Invitrogen, 13778150) were suspended in 100 μl of water (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were fed daily by complete medium exchange with Essential 6 medium (E6, #A1516401, Thermo Fisher Scientific) in the presence of 100 nM LDN193189 (#72142 ...
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bioRxiv - Cancer Biology 2022Quote: ... real-time quantitative PCR was done using the ABI Quant Studio 5 and 6 (Life technologies, USA). The cDNA was used as template and DyNAmo Flash SYBR Green qPCR kit (#F-416L ...