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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Recombinant Human BTLA Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant fibroblast growth factor (human FGF2) (10 ng/ml, Invitrogen) was added to Dulbecco’s Modified Eagle’s Medium (DMEM)/F-12 (Gibco) ...
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bioRxiv - Cell Biology 2019Quote: Recombinant GST and GST-tagged EB1 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.7 and induced with 1 mM IPTG for 5.5 h at 25°C ...
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bioRxiv - Immunology 2022Quote: ... and the MERS-CoV S protein with Avi-tag was conjugated to streptavidin-AF647 (Invitrogen cat.# S21374). After incubating the cells with Ab mixture for cell surface markers for 15 min in dark ...
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bioRxiv - Microbiology 2020Quote: Recombinant biotinylated proteins (sE, sE-cvD and DIII) were expressed at 28°C using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Immunology 2020Quote: ... Plates were then incubated with 1μg/ml of biotinylated recombinant ACE2 protein for 1 hour at room temperature followed by incubation with HRP-conjugated streptavidin (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... the microtiter plates were washed 5 × with T-PBS and then incubated for 1 hour at room temperature with 2 μg/ml of biotinylated recombinant protein G (PierceTM, Thermo Fisher Scientific) for recognition of IgG antibodies from human ...
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bioRxiv - Cell Biology 2019Quote: Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were cultured on Vitronectin (VTN-N) recombinant human protein (ThermoFisher Scientific, A14700). Culture vessels were coated with 200μL/cm2 of Vitronectin that had been diluted to 6μg/ml with PBS and incubated at 37°C for at least 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... Myc-tagged proteins were immunoprecipitated with Protein G Dynabeads (Invitrogen, 10003D) coated with anti-myc antibody (Cell Signaling ...
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bioRxiv - Immunology 2021Quote: Purified human MYC- or Fc-tagged NKp46 were labeled with Alexa647 using the microscale protein labeling kit (ThermoFisher Scientific). Alexa647-labeled NKp46-Myc (50 nM ...
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bioRxiv - Cancer Biology 2022Quote: Recombinant human IFNγ (PHC4031) and recombinant mouse IFNγ (PMC4031) were purchased from Gibco and used at the indicated concentrations ...
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bioRxiv - Microbiology 2024Quote: ... protein expression from pBAD-His/B (Invitrogen) derivative plasmids was induced at the temperature shift by adding 0.01–0.2% L-arabinose ...
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bioRxiv - Bioengineering 2023Quote: ... The biotinylated recombinant toxins were captured using streptavidin-coated Dynabeads (Invitrogen, M-280). After washing with PBS containing 0.1% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... while the digestion cocktail for RNase HI-positive samples were supplemented with 10 U recombinant Escherichia coli RNase HI (Invitrogen). After digestion ...
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bioRxiv - Microbiology 2022Quote: Recombinant human CD81 protein (R &D Systems) was coated (4µg/ml) on ELISA plate (Thermo Fisher Scientific) and incubated overnight at 40C ...
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bioRxiv - Cell Biology 2024Quote: ... Expression of the recombinant LZTR1 proteins was confirmed by Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific). Following confirmation of expression ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged RRs were purified from clarified lysate using HisPur Ni-NTA resin (Thermo Scientific) or HisPur Cobalt resin (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged LCRIS_00661 and LCRIS_00558 genes with the appropriate restriction sites were ordered from Invitrogen and subcloned into a previously constructed S ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/ml recombinant human GDNF (Invitrogen, USA) and 1% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2021Quote: ... 20 ng/mL human recombinant EGF (Thermo Fisher), 10 ng/mL cholera toxin ...
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bioRxiv - Immunology 2022Quote: ... 50 ng/mL recombinant human EGF (Gibco, PHG0314), 80 ng/mL recombinant human R- spondin 1 (R & D systems ...
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bioRxiv - Neuroscience 2021Quote: Human recombinant IGF1 was purchased from Thermo Fisher, and K252a ...
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bioRxiv - Cancer Biology 2020Quote: ... and 10 Units/mL human recombinant insulin (Gibco). All cell culture was conducted in a class two ...
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bioRxiv - Cancer Biology 2020Quote: ... Human recombinant EGF was from Invitrogen (cat # PHG0311) or PeproTech (cat # AF-100-15) ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human bFGF (Cat# 13256029) purchased from Gibco; LY294002 (Cat# PHZ1144 ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μg/mL human recombinant insulin (Thermo Fisher), and 1 μg/mL hydrocortisone (Sigma Aldrich) ...
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bioRxiv - Biophysics 2022Quote: ... Recombinant human insulin was purchased from Thermo Fisher USA ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10 IU/ml human recombinant insulin (Gibco). OAW28 was grown in Advanced DMEM (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 ng/mL human recombinant EGF (Gibco, #PHG0314), 20 ng/mL human recombinant FGF (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 ng/mL recombinant human EGF (Gibco #PHG0313), 10 ng/mL basic-FGF (Millipore #GF003-AF) ...
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bioRxiv - Neuroscience 2020Quote: ... human recombinant brain-derived neurotrophic factor (Life Technologies), penicillin/streptomycin ...
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bioRxiv - Cell Biology 2019Quote: ... 10 ng/mL recombinant human EGF (Thermo Fisher), 25 ng/mL prostaglandin E1 (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... 20 ng/ml human recombinant EGF (Gibco, #PHG0311L), 100 ng/ml Cholera Toxin (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/mL human recombinant EGF (ThermoFisher PHG0311), Antibiotic-Antimycotic (Gibco 15240-062) ...
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bioRxiv - Immunology 2021Quote: ... Recombinant human IL-2 was purchased from ThermoFisher Scientific.
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bioRxiv - Neuroscience 2022Quote: ... 10μg ml−1 human recombinant insulin (GIBCO, 12585014).
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bioRxiv - Cell Biology 2023Quote: ... exposed to recombinant human TGFβ1 (Thermo Fisher 7754BH005) 2ng/ml for 3 days ...
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bioRxiv - Biophysics 2023Quote: ... The recombinant human TGF-β1 (Invitrogen, MD, USA) was added at a final concentration of 1 ng/mL ...
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bioRxiv - Cell Biology 2023Quote: ... human recombinant laminin (0.2 mg/ml, Life Technologies), Y-27632 (10 mM ...
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bioRxiv - Developmental Biology 2023Quote: ... 12.5 µg/mL human insulin recombinant zinc (Gibco), 20 ng/mL leukaemia inhibitor factor (Cambridge Stem Cell Institute) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10ng/ml recombinant human IL-3 (Gibco). Cultured cells were infected with lentivirus encoding GFP/Scrambled control shRNA overnight and fresh media was added the following day ...
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bioRxiv - Cancer Biology 2024Quote: ... 20ng/mL recombinant human FGF-basic (Fisher Scientific). For each patient sample ...
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bioRxiv - Bioengineering 2024Quote: ... and 5 ng/mL Human Recombinant EGF (Gibco) following manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 20ng/mL recombinant human FGF-basic (Fisher Scientific)] ...
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bioRxiv - Molecular Biology 2021Quote: ... cerevisiae EKY3/pYES2-TOP1 induced for expression of wild-type human TOP1with the PrepEase Histidine-Tagged Protein Purification Midi Kit – High Specificity (from Affymetrix) according to the manufacturer’s procedures ...
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bioRxiv - Neuroscience 2022Quote: ... HeLa cells were transfected with green fluorescent protein (GFP)-tagged human NCKAP1 cDNA or NCKAP1 shRNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: 1-2 μg His-tagged calcineurin was first bound to magnetic Dynabeads (Thermo Fisher Sci. USA) in base buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The His tagged RBD construct was purified from the culture supernatant of Expi293 cells (Thermo Fisher) that were transfected with the plasmid DNA using FectoPRO (Polyplus Inc.) ...
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bioRxiv - Plant Biology 2023Quote: ... His-tagged AtKu70/80 was isolated from the supernatant using HisPur Ni-NTA resins (Thermo Fisher) and dialysed in 20 mM HEPES pH7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... contained 40 µM human Flag-tagged ubiquitin (Fisher Scientific U-120), 0.5 µM human His6-tagged Ubiquitin E1 enzyme (Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.5 µM human His6-tagged Ubiquitin E1 enzyme (Fisher Scientific, E304050), 2.4 µM human His6-UBE2T E2 enzyme (Fisher Scientific ...