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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Rat T Cell Surface Glycoprotein CD8 Beta Chain CD8B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were isolated using a commercial kit (MagniSort Human CD4+ T Cell Enrichment; Affymetrix) and infected by incubation for 4 h at 37°C with 156,250 or 2,500 TCID50 (50% tissue culture infectious dose ...
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bioRxiv - Immunology 2019Quote: ... total CD4+ T cells were isolated using the Dynabeads CD4 positive T cell isolation kit (Invitrogen). Purified cells were 95%–98% pure as determined by flow cytometry analysis ...
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bioRxiv - Microbiology 2023Quote: Proteins on the surface of Env-expressing HEK293T cells were biotinylated with the Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Immunology 2022Quote: ... and then subjected to measurement of IL-1β protein using the Mouse IL-1 beta-uncoated ELISA kit (Invitrogen, Waltham, MA), following manufacturer’s instructions.
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bioRxiv - Neuroscience 2019Quote: Aß1-40 and Aß1-42 concentrations from cleared supernatants of differentiated iPSCs were quantified by using the Amyloid beta 40/ 42 Human ELISA Kits (#KHB3441 and KHB3481; Thermo Fisher) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2019Quote: ... Human T-cells isolated were also used to generate T-cells blasts using the Dynabeads Human T-Activator CD3/CD28 for T-cell expansion and Activation kit (Life Technologies) as per company’s protocol ...
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bioRxiv - Genetics 2020Quote: ... cells were surface stained with cell-viability dye (Fixable Blue dead cell stain kit, Invitrogen), followed by anti-CD3-BV711 (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... incorporation of glucose and fatty acid metabolism was determined by flow cytometry analysis of isolated CD8+ T cells from PLWH and control donors stained with 2-NBDG (Invitrogen) and 500/510 BODIPY (Molecular Probes ...
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bioRxiv - Immunology 2019Quote: Intracellular ATP levels were measured ex vivo on sorted CD4+ and CD8+ T cell subsets via a bioluminescence assay according to the manufactures instructions (ThermoFisher).
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bioRxiv - Immunology 2020Quote: ... naïve CD8+ T-cells were isolated from an enriched PBMC fraction and activated with αCD3 and αCD28 beads (Dynabeads, Invitrogen) for 48 hours ...
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bioRxiv - Bioengineering 2019Quote: CD8+ and CD4+ T cells were purified from PBMC and activated by stimulation with anti-CD3/anti-CD28 beads (Thermofisher) in separate cultures ...
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bioRxiv - Genetics 2019Quote: ... Non-adherent cells in suspension from resting and PHA-stimulated cultures were removed and purified for CD8−/CD4+ T cells using Dynabeads (Invitrogen) and stored in RNAprotect Cell reagent (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... non-naïve (CCR7+CD45RA-, CCR7-CD45RA- and CCR7-CD45RA+) CD3+CD8+ T cells were directly sorted into TRIzol reagent (Thermo Fisher) on a FACSAriaFusion (BD ...
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bioRxiv - Immunology 2022Quote: ... freshly isolated or cryopreserved human PBMC or purified CD8+ T-cells (1×106) were cultured in complete culture medium (RPMI 1640 (Gibco) supplemented with 10% FCS ...
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bioRxiv - Microbiology 2023Quote: ... the infected cells were co-cultured with HLA-B/CMV-restricted CD8+ T-cells for five hours (E:T ratio: 3:1) in RPMI 1640 medium (Life Technologies)(supplemented with 10 % (v/v ...
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bioRxiv - Immunology 2022Quote: ... Nuclear and cytoplasmic extracts from CD8+ T cells were obtained with the NE-PER Nuclear and Cytoplasmic Extraction Reagents (78833, ThermoFisher). The Revert 700 Total Protein Stain (LICOR ...
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bioRxiv - Bioengineering 2023Quote: ... 105 rested NY-ESO-1 TCR+ T cells (4:1 CD8+:CD4+) were labeled with 0.5uM CSFE (Thermo Fisher Scientific) and co-cultured with 105 NucLight red+ tumor cells in complete medium ...
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bioRxiv - Cancer Biology 2020Quote: ... Proliferation of CD4+ and CD8+ T cells was induced by stimulating cells with plate bound anti-CD3 [0.1 µg/mL] (Thermo Fisher Scientific, MA, USA) and soluble anti-CD28 [1 µg/mL] (BD Biosciences ...
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bioRxiv - Plant Biology 2022Quote: ... pastoris-produced LysM protein solution was tested using a protein polyacrylamide gel followed by CBB and glycoprotein staining with the Pierce Glycoprotein Staining Kit (Thermo Fisher Scientific, California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... T cells were purified from splenocytes using a negative selection kit (MagniSort T cell enrichment, Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were isolated from human PBMCs with an Untouched Human T-cell Isolation Kit (Invitrogen, USA) and activated with Dynabeads Human T Activator CD3/CD28 (Gibco ...
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bioRxiv - Physiology 2021Quote: The concentrations of IL-1β in tissue extracts of ovaries and livers were determined using an IL-1 beta Pro-form Mouse Uncoated ELISA kit (88-8014-22; Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... A rat-specific primary antibody for skeletal muscle myosin heavy chain (MA1-35718, ThermoFisher) was diluted 1:5 in TBS-NGS ...
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bioRxiv - Microbiology 2021Quote: ... After 4x washing with PBS-T the ELISA was developed with 1-step Ultra TMB ELISA (Thermo Scientific). For generation of an oxMIF standard curve ...
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bioRxiv - Plant Biology 2023Quote: ... Surface-exposed proteins were biotinylated and purified using the Pierce™ Cell Surface Protein Biotinylation and Isolation Kit (ThermoFisher Scientific), following the manufacturer’s protocol with some modifications ...
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bioRxiv - Cell Biology 2024Quote: EV surface proteins were captured using biotinylated using Pierce™ Cell Surface Biotinylation and Isolation Kit (A44390, Thermo Fisher Scientific), digested with trypsin (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... Compensation controls used splenocytes for most cell surface markers and compensation beads (BD Biosciences, 552844 for rat hosts; ThermoFisher, 01-3333-41 for mouse or Armenian hamster hosts ...
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bioRxiv - Cell Biology 2023Quote: ... The surface of the gel was then rendered permissive to cell adhesion by coating Rat Tail collagen I (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... The following ELISA kits were used: IL-1β Mouse ELISA kit (ThermoFisher), IL-18 Mouse ELISA kit (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... Secondary anti-rabbit Alexa Fluor 488 and T cell isolation kit (Dynabeads™ Untouched™ Mouse T Cells Kit, Cat. no. 11413D) were procured from Invitrogen, Carlsbad ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with unconjugated lineage rat antibodies (CD3, CD4, CD5, CD8, B220, Gr1, Mac1, and Ter119) followed by goat-α-rat PE-Cy5 (Invitrogen). Stem and progenitor cells were isolated using fluorescently labeled or biotinylated antibodies for the following antigens ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells were loaded with 5 μM CellTrace CFSE or CellTrace Violet (CTV) dyes (Thermo Fisher, C34554 and C34557, respectively) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Naive CD8+ T cells were isolated from Spleen and Lymph Nodes using two rounds of negative depletion with Streptavidin DynaBeads (Thermo Fisher) using 1*108 and 4*107 beads per 1*108 total splenocytes/LN cells ...
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bioRxiv - Immunology 2019Quote: ... CD8+ T-cells were loaded with 5 µM CellTrace CFSE or CellTrace Violet (CTV) dyes (Thermo Fisher, C34554 and C34557, respectively) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... cell materials derived from SSChigh group (day 13) which was enriched in large numbers of terminally differentiated CD8+ T cells were labelled with CellTrace Far Red (Invitrogen, C34564) following the manufacturer protocol ...
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bioRxiv - Immunology 2021Quote: ... total RNA was isolated from either 1.5×106 SEA stimulated CTL or MART-1-specific CD8+ T-cell clones using TRIzol reagent (Thermo Fisher Scientific) containing 1 µl Glycogen (5 µg/µl ...
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bioRxiv - Immunology 2021Quote: ... Naïve CD8+ T cells were then activated in vitro using Dynabeads™ conjugated with anti-CD3 and anti-CD28 monoclonal antibodies (Gibco), for a total of 48h ...
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bioRxiv - Cancer Biology 2024Quote: ... B7-H3.CAR-T cells or patient derived CD8+ expanded TILs were activated with washed aCD3/aCD28 dynabeads (ThermoFisher Scientific, 11161D), according to the manufacturer protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... using ELISA kits (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... commercial ELISA kits (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... ELISA kits by Invitrogen (mouse Aβ42 ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells and NK cells were isolated from cryopreserved PBMCs using commercial kits (MagniSort Human CD4+ T Cell Enrichment; Affymetrix, and MagniSort™ Human NK cell Enrichment ...
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bioRxiv - Immunology 2020Quote: Peripheral T cells were isolated from mouse spleen by a CD4+ T-cell negative (Stem Cell) or positive selection kit (Invitrogen). Enriched CD4+ T cells were further sorted for either YFP+ (Foxp3+ ...
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bioRxiv - Immunology 2019Quote: ... T cells were isolated using Dynabeads Untouched Human T Cells Kit using manufacturer’s protocols (Thermo Fisher, Waltham, MA). Isolated T cells were kept in T cell media ...
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bioRxiv - Immunology 2021Quote: Isolation of CD3+ T-cells was performed using Dynabeads® Untouched™ Human T-cells Kit (11344D, Invitrogen). CD3+ T-cells were stained with CellTrace Violet Cell Proliferation Kit (C34571 ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were isolated from PBMCs using the Dynabeads® Untouched Human T Cells Kit (Invitrogen, Karlsruhe, Germany) following the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... and on the surface of H1568 EVs (with IFN-γ stimulation) were quantified using a PD-L1 Human ELISA kit (#BMS2212, ThermoFisher Scientific). For the cells ...
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bioRxiv - Microbiology 2021Quote: ... the BrdU-labeled splenocytes were surface stained for T-cell (CD3e-APC; eBioscience) and B-cell (CD19-eFluor450, ThermoFisher Scientific, Grand Island, NY) markers after blocking with anti-mouse CD16/32 antibodies (BioLegend) ...
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bioRxiv - Neuroscience 2021Quote: ... Transfected cells were identified by CD8 expression was detected with CD8 Dynabeads (Life Technologies). Whole-cell currents were recorded in voltage-clamp mode in the following solutions ...
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bioRxiv - Immunology 2022Quote: ... glycoproteins were digested with trypsin (ThermoFisher) overnight at 37°C ...