Labshake search
Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Mouse OLFR139 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2020Quote: ... This plasmid was expanded with PureLink™ HiPure Plasmid Midiprep Kit (Invitrogen), digested with BamHI (NEB) ...
-
bioRxiv - Biochemistry 2021Quote: ... Plasmids were purified using the GeneJET plasmid miniprep kit (Thermo Fisher Scientific) and verified by Sanger sequencing ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Plasmids were extracted using a GeneJet Plasmid Miniprep kit (Thermo Fisher Scientific), linearised using HindIII to avoid supercoiling ...
-
bioRxiv - Immunology 2022Quote: ... and plasmids were prepared using (Invitrogen, PureLink™ HiPure Plasmid Midiprep Kit), following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... Plasmid DNA was isolated using the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific). DNA was sequenced by ELIM Biopharm (Hayward ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The plasmid DNA was extracted using GeneJET Plasmid Miniprep kit (Thermo Scientific) and subjected to sequencing ...
-
bioRxiv - Neuroscience 2023Quote: ... Plasmids were isolated using the PureLink HiPure Plasmid Maxiprep kit (ThermoFisher Scientific), eluted in sterile water ...
-
bioRxiv - Immunology 2023Quote: ... Plasmids were purified using the PureLink HiPure Plasmid Maxiprep Kit (Thermo Fisher) followed by the MiraCLEAN Endotoxin Removal Kit (Mirus ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids were extracted using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). DNA quantification was done using the Multiskan GO spectrophotometer instrument (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmid cDNA was purified using GeneJET Plasmid Maxiprep kits (Thermo Fisher Scientific) according to the manufacturer’s instructions.
-
bioRxiv - Biochemistry 2023Quote: ... Plasmids were purified using the GeneJET plasmid miniprep kit (Thermo Fisher Scientific) and insertion and mutagenesis were verified by Sanger sequencing.
-
bioRxiv - Systems Biology 2023Quote: ... The plasmid DNA was extracted by PureLink™ Plasmid Purification Kit (Invitrogen). The library was sequenced to confirm uniform and complete library representation.
-
bioRxiv - Genomics 2023Quote: ... The repair plasmid (repair plasmid 1) was synthesized commercially (GeneArt; Life Technologies) with recodonised sequences in the 2986–3024 bp and 3273–3305 bp regions of the pfap2-mrp and 400 bp homology arms flanking the 5’ and 3’ regions of the intron ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The plasmid was purified using the GeneJET Plasmid Miniprep Kit (Thermo Scientific) and assembly junctions were verified by Sanger sequencing ...
-
bioRxiv - Biophysics 2024Quote: ... and plasmids were purified using a PureLink Plasmid Miniprep Kit (Invitrogen, #K210011) or a Qiagen Plasmid Midi Kit (Qiagen ...
-
bioRxiv - Microbiology 2024Quote: ... The cloned plasmids were isolated with GeneJET Plasmid Miniprep Kit (Thermo Scientific). Sequencing was performed at Eurofins Genomics ...
-
bioRxiv - Microbiology 2024Quote: ... The cloned plasmids were isolated with GeneJET Plasmid Miniprep Kit (Thermo Scientific) or QIAprep Spin Miniprep Kit (QIAGEN) ...
-
bioRxiv - Genomics 2022Quote: ... pMT-V5 plasmid (Invitrogen) was cut using KpnI and XhoI ...
-
bioRxiv - Cell Biology 2019Quote: ... pcDNA4-MycHisB plasmid (ThermoFisher) was digested with HindIII and EcoRI ...
-
bioRxiv - Genetics 2021Quote: ... linearized pUC19 plasmid (Invitrogen) using the NEBuilder® HiFi DNA Assembly Cloning Kit (NEB ...
-
bioRxiv - Biophysics 2022Quote: ... the pPIC9K plasmid (Invitrogen) was mutated using Q5 Site-Directed Mutagenesis (New England BioLabs ...
-
bioRxiv - Immunology 2022Quote: ... a pSecTAG2 plasmid (ThermoFisher) was produced ...
-
bioRxiv - Microbiology 2024Quote: ... with plasmid pUC19 (Invitrogen) containing 12.8 kbp DNA (total size is 15.5 kbp ...
-
bioRxiv - Cancer Biology 2021Quote: ... Hep3B2 cells were transfected with 4 μg of HKDC1 shRNA construct (Dharmacon; RHS4740-EG80201) using Lipofectamine LTX with Plus Reagent (Invitrogen; A12621). 48 hours post transfection transfected cell were selected with puromycin (0.5 μg/ml ...
-
bioRxiv - Neuroscience 2021Quote: ... We generated five different shRNA constructs targeting each of the target genes using the Block-iT RNAi Designer web tool (Thermo Scientific) to identify target sequences within the coding region of the genes that show high knockdown effectivity in silico ...
-
bioRxiv - Molecular Biology 2019Quote: ... shCyb5r3 adenovirus was generated by cloning the effective shRNA sequence (5’-GATTGGAGACACCATTGAAT-3’) into the pEQU6-vector with LR Clonase II (ThermoFisher 11791100), then ligating to pAd-REP ...
-
bioRxiv - Bioengineering 2020Quote: ... Knockdown efficiency in the T cells following shRNA transduction was determined by real-time quantitative PCR with TaqMan gene expression assays (Applied Biosystems) for TET2 (assay Hs00325999_m1 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and incubated for 15-40 min before adding MYCN-amplified IMR5/75 cells stably expressing a doxycycline-regulable MYCN shRNA (2,100 cells/well) using a Multidrop dispenser (Thermo Fisher Scientific). Two treatment conditions were screened in triplicate ...
-
bioRxiv - Biochemistry 2022Quote: ... an shRNA-generating sequence equivalent to the above siRNA sequence was inserted into the pSilencer 4.1 CMV-Hygro vector (Ambion, Austin TX). The plasmid was transfected into Neuro2a cells and clonal lines selected by incubation with Hygromycin (300 mg/ml ...
-
bioRxiv - Cancer Biology 2022Quote: ... Sequencing libraries were prepared by subjecting 18 μg (1.2 μg x 15 reactions) of genomic DNA (∼1000x shRNA representation) to PCR based barcoding and amplification with AmpliTaq Gold PCR kit (ThermoFisher Scientific). See Supplementary Table 3 for list of barcode adaptor primers ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells in quadruplicate wells were transfected with 2 μg DNA of either TMEM163-KD (knock-down) shRNA or TMEM163-OE (over-expression) construct using TurboFect lipid reagent (Thermo Scientific). Twenty-four hours post-transfection ...
-
bioRxiv - Developmental Biology 2022Quote: Animals injected with either shRNA #1 or #2 were collected at 48hpf and lysed in 500 µl of TRIzolTM reagent (Invitrogen, 15596026) by vortexing extensively ...
-
bioRxiv - Cancer Biology 2023Quote: ... Zbtb46 knockdown MCEC and 1956 cell lines were generated by transducing the parental MCEC and 1956 cells with lentiviral particles from a set of 5 shRNA clones for Zbtb46 followed by puromycin selection (Cat: A1113802, ThermoFisher Scientific). GFP expressing Lewis lung carcinoma (LLC cells ...
-
bioRxiv - Cell Biology 2023Quote: ... 1.2 × 106 cells were transfected with 1 μg DNA or shRNA for each construct using the Neon transfection system (Life Technologies) following the manufacturer’s instructions (1200 pulse voltage ...
-
bioRxiv - Immunology 2023Quote: ... mouse anti-mouse PCSK9 (Invitrogen), mouse anti-mouse vinculin (Santa Cruz) ...
-
bioRxiv - Genomics 2024Quote: ... Tir1-BirA mouse ES cells at a confluency of 70-80% were transfected with the plasmid constructs using Lipofectamine 2000 (ThermoFisher Scientific, Cat#11668019) following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... transfection with TOL2-GFP-T2A-TGIF1 overexpression plasmid or control plasmid and transposase expressing plasmid was performed following the Lipofectamine 2000 protocol (Life Technologies). Cells were collected 48 hours post transfection and Trizol RNA extraction was performed as described before.
-
bioRxiv - Physiology 2024Quote: ... and TaqMan primers (mouse Panx1 mm0045091, mouse Panx2 mm01308054, mouse Pan×3 mm00552586, mouse Gja1 mm00439105, mouse Gja5 mm00433619– Invitrogen) (Rat Panx1 Rn01447976_m1 – Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... Plasmids for transfection were purified using PureLink™ HiPure Plasmid Midiprep Kit (Invitrogen).
-
bioRxiv - Genetics 2019Quote: ... The plasmid library was purified with a GeneJET Plasmid Midiprep Kit (Thermo Scientific).
-
bioRxiv - Genomics 2020Quote: ... Bacteria were harvested and plasmids isolated using GeneJet Plasmid Miniprep kit (Thermo Scientific). PCR was performed to evaluate clone inserts ...
-
bioRxiv - Synthetic Biology 2021Quote: gRNA expression plasmids were purified using PureLink HiPure Plasmid Midiprep Kit (Invitrogen K210004) following manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... Plasmid material was isolated using the GeneJet plasmid miniprep kit (Thermo Fisher Scientific), sequence verified (GATC-biotech ...
-
bioRxiv - Biochemistry 2020Quote: Plasmid DNA was extracted with the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... repair template plasmids were isolated with the GeneJET Plasmid Miniprep Kit (Thermo Scientific) from the overnight E ...
-
bioRxiv - Microbiology 2021Quote: Plasmid DNA was routinely purified using the GeneJet plasmid miniprep kit (Thermo Fisher). PCR was conducted with Phusion HiFi polymerase (Thermo Fisher) ...
-
bioRxiv - Neuroscience 2019Quote: ... plasmid was isolated using GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific Inc., USA). DNA sequencing was performed in both directions using universal primers for M13 which flanks the multiple cloning site of the plasmid ...
-
bioRxiv - Cell Biology 2022Quote: ... Plasmids were extracted using the PureLinkTM HiPure Plasmid Maxiprep Kit (Thermo Fisher Scientific) with the PureLinkTM HiPure Precipitator module (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: ... the plasmids were isolated by using GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) and sent for sequencing to confirm the correct assembly ...
-
bioRxiv - Microbiology 2022Quote: ... and the plasmids were isolated using the GeneJET Plasmid Miniprep kit (Thermo Fisher). DNA sequencing was carried out to assess the fidelity of the mutagenesis reaction.