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Citations for Thermo Fisher :
201 - 250 of 2759 citations for IFNAR1 Rhesus Macaque HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The pSAd36-S and pSAd-control plasmids were linearized with PacI restriction enzyme to liberate viral genomes for transfection into T-Rex 293-HEK cells (Invitrogen). The rescued replication-incompetent ChAd-SARS-CoV-2-S and ChAd-Control vectors were scaled up in 293 cells and purified by CsCl density-gradient ultracentrifugation ...
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bioRxiv - Immunology 2021Quote: ... Expression vector was transiently transfected into Human Embryonic Kidney (HEK) 293 GnTI-Freestyle cells suspension culture in serum-free media (Invitrogen) using polyethyleneimine (Polysciences) ...
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bioRxiv - Microbiology 2021Quote: Human endothelial kidney (HEK 293) and murine fibroblast (3T12) cells were cultured in Dulbecco’s modified Eagle medium (DMEM; Life Technologies) supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: The viability of HEK-293 cells post exposure to films was also determined using a LIVE/DEAD Viability/Cytotoxicity Kit (Invitrogen). Approximately 105 cells were seeded per well ...
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bioRxiv - Molecular Biology 2022Quote: pcDNA5/FRT/TO-FLAG-Angel1 WT was used to generate stable HEK FLp-In T-REx-293 cell lines (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% Foetal Bovine Serum (FBS) (HEK 293) or Calf Serum (NIH 3T3 and Ptch1−/− MEFs) containing 1% Glutamax (Gibco). All cell lines were grown at 37°C in 5% CO2 and passaged prior to reaching confluence.
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bioRxiv - Cancer Biology 2023Quote: ... HEK-293 (CRL-1573) cells were obtained from ATCC and maintained in high-glucose (4.5 g/L) DMEM (Gibco 11995073) supplemented with 4 mM L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... lentiviruses (for target sequences see S1 File) for shRNA delivery were produced by transfecting HEK-293 cells in a 60mm dish with a 1ml OptiMEM (Gibco) mix containing 15µl of Lipofectamine-2000 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... with fewer than 15 passages onto 12-well plates in HEK-293 growth medium (DMEM [Gibco # 10566-061] with 10% FBS [Gibco #16140-071] and 1x Pen Strep [Gibco #15070-63] ...
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bioRxiv - Neuroscience 2023Quote: ... we plated HEK-293 cells (ATCC catalog # CRL-1573) with fewer than 15 passages onto 12-well plates in HEK-293 growth medium (DMEM [Gibco # 10566-061] with 10% FBS [Gibco #16140-071] and 1x Pen Strep [Gibco #15070-63] ...
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bioRxiv - Biophysics 2024Quote: The original CC1 construct with super ecliptic pHluorin (SE) A227D (31) or CC1 variants with mutations in S1 were expressed in HEK 293 cells via lipofectamine (Invitrogen). Coverslips with transiently transfected cells were placed into a bath chamber (Warner instruments ...
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bioRxiv - Cell Biology 2020Quote: ... Identified and characterized plasmids containing the ACE2 isoform open reading frames were transfected into the HEK-293 cells using TurboFect reagent (ThermoFisher Corp.). 24 hours post-transfection the cells were harvested and processed for analysis by western blot according to established procedures [21] ...
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bioRxiv - Cell Biology 2022Quote: ... Ciliogenesis was induced by culturing RPE1 cells in medium without serum during 48 hours. HEK 293 cells (kind gift from F. Causeret, Institut Imagine, Paris) were cultured in DMEM medium (ThermoFisher Scientific) supplemented with 10 % fetal calf serum and antibiotics as previously ...
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bioRxiv - Neuroscience 2021Quote: ... and Human Embryonic Kidney 293 (HEK) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies- Invitrogen, Carlsbad, CA, USA). Both media were supplemented with 10% Fetal Bovine Serum Gold (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... and Human Embryonic Kidney 293 (HEK) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies- Invitrogen, Carlsbad, CA, USA). Both media were supplemented with 10% Fetal Bovine Serum Gold (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... the HEK 293 BRI3BP-HA cell line was transfected with control or BRI3BP silencer select siRNAs with Lipofectamine RNAi Max (ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... DMEM (for A-431, HEK-293, NTERA-2, SH-SY5Y) or Ham’s F-12K (for A549) media (by Gibco or Euroclone) containing 10% fetal calf serum ...
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bioRxiv - Microbiology 2022Quote: 100µg of the pCI PR8 NP:WT-C-His or pCI PR8 NP:F346S-C-His plasmids were transiently transfected into 100mL cultures of HEK Expi-293-F cells with ExpiFectamine according to the company protocol (Thermo Fisher). The transfected cells were pelleted (1000rpm ...
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bioRxiv - Genomics 2021Quote: ... Reporter plasmids (2 μg) were transfected into HEK-293 cells in 6-well plates at ∼60-90% confluency using Lipofectamine 3000 (ThermoFisher Scientific). Cells were harvested after 1 day by aspirating the media and resuspending the cells in 1 mL Trizol reagent (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... HEK-293 cells were maintained at 37°C with 5% CO2 in DMEM/F12 medium (Gibco, Life Technologies, Grand Island, NY) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... HEK-293 cells were maintained at 37°C with 5% CO2 in DMEM/F12 medium (Gibco, Life Technologies, Grand Island, NY) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Biochemistry 2022Quote: The pEG BacMam plasmids with WT or mutant Fpn or the empty plasmid were transfected into HEK 293S cells on black wall 96-well microplates coated with poly-D-lysine (Invitrogen/Thermo Fisher). After 2 days ...
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bioRxiv - Biochemistry 2022Quote: The pEG BacMam plasmids with HsFpn or the empty plasmid were transfected into HEK 293S cells on black wall 96-well microplates coated with poly-D-lysine (Invitrogen/Thermo Fisher). After 2 days ...
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bioRxiv - Cell Biology 2022Quote: ... MEFs were cultured in the same medium as HEK 293 cells but supplemented with 1x non-essential amino acids (Gibco, 11140050). All cells were kept at 37°C in a 5% CO2 humidified atmosphere.
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bioRxiv - Cell Biology 2023Quote: ... HEK 293-TrkB cells were treated with drugs prior to cell lysis with cold Pierce RIPA lysis buffer (Thermo Scientific, 89901) supplemented with protease inhibitor set I (Sigma ...
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bioRxiv - Cell Biology 2023Quote: For proximity ligation assay transfected HEK-293 cells were seeded in an appropriate dilution on Nunc Chamber Slides (Thermo Fisher, USA). After fixation in 4% paraformaldehyde for 15 min at RT ...
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bioRxiv - Bioengineering 2024Quote: ... and m6A-Tracer-NES plasmid (m6A-Tracer; addgene, #159607) were simultaneously transfected into HEK-293 cells using Lipofectamine 3000 Transfection Reagent (Invitrogen, #L3000008). After 48-h co-transfection ...
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bioRxiv - Systems Biology 2020Quote: ... 293-H and Freestyle™ 293-F (Gibco) were obtained from Thermo Fisher Scientific and cultivated in 293 SFM II medium (Gibco ...
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bioRxiv - Neuroscience 2023Quote: The plasmid for heterologous expression of fshr-1 in HEK cells was obtained by directionally cloning the fshr-1 cDNA into the pcDNA3.1/V5-His-TOPO vector (Invitrogen). The cDNA sequence of the fshr-1a gene isoform was amplified by PCR using cDNA from mix-staged wild-type C ...
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bioRxiv - Neuroscience 2021Quote: ... 3-3.5 µg prestin plasmids and 0.4 µg eGFP plasmid were transfected to HEK 293 cells using 10 µl of Lipofectamine ® 3000 (reagent 2:1 ratio; ThermoFisher Scientific) in 500-ml Opti-MEM (Life Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: The dual pri-miRNA expression plasmids were transfected to human embryonic kidney (HEK) 293 cells in 6-well plates using the Lipofectamine 3000 reagents (Thermo Fisher Scientific) following standard procedures ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: HEK-293 cells or HEK-293 cells stably expressing PAR4-YFP were loaded with calcium sensitive dye (Fluo-4 NW, F36206, Life Technologies, Thermo Fisher) and aliquoted into cuvettes with Hanks buffered salt solution (HBSS containing Ca2+ and Mg2+) ...
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bioRxiv - Bioengineering 2021Quote: ... WS1 and HEK-293 cells were obtained from ATCC (Manassas, VA) and cultured in Eagle’s Minimum Essential Medium (Life Technologies, Carlsbad, CA) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... HEK 293 cells or rNLS mice brains were lysed in lysis buffer (50 mM Tris-HCl, protease inhibitors cocktail (Thermo Fisher Scientific), phosphatase inhibitor (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... HEK-293 cells (ATCC) were maintained at 37°C under 5% CO2 in Dulbecco’s modified Eagle’s medium (Gibco, Cat. no. 12800-017) supplemented with 10% FBS (Gibco ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... HEK-293 cells were transferred with a density of 12000 cells per well in white 96-well plates (Thermo Scientific™ Nunc™ F96 MicroWell™ ...
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bioRxiv - Biochemistry 2022Quote: The pEG BacMam plasmids with WT or mutant Fpn or the empty plasmid were transfected into HEK 293S cells on black wall 96-well microplates coated with poly-D-lysine (Invitrogen/Thermo Fisher). After 2 days ...
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bioRxiv - Biochemistry 2022Quote: The pEG BacMam plasmids with HsFpn or the empty plasmid were transfected into HEK 293S cells on black wall 96-well microplates coated with poly-D-lysine (Invitrogen/Thermo Fisher). After 2 days ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting plasmids with Fpn or the empty plasmid were transfected into HEK 293S cells on 6-well plates with 293fectin™ transfection reagent (Invitrogen/Thermo Fisher) per the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: HeLa and HEK 293 cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Life Technologies, Thermo Fisher Scientific, Waltham MA, USA), while SH-SY5Y cells were cultured in 1:1 DMEM:Ham’s F12 (MSD ...
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bioRxiv - Genetics 2023Quote: ... Reverse transfection in HEK 293 cells was performed with 3 nM final siRNA concentration with Lipofectamine™ RNAiMAX (ThermoFisher, Cat. No. 13778075) according to manufactural procedure ...
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bioRxiv - Immunology 2023Quote: ... Consensus and Vietnam matrix protein 2 (M2)-inducible HEK cells were generated using the Flp-In™ T-REx™ 293 Cell Line system (Invitrogen) and grown following the methods in the previous papers 53 ...
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bioRxiv - Neuroscience 2023Quote: ... with fewer than 15 passages onto 12-well plates in HEK-293 growth medium (DMEM [Gibco # 10566-061] with 10% FBS [Gibco #16140-071] and 1x Pen Strep [Gibco #15070-63]) and transfected them when 50-70% confluent ...
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bioRxiv - Immunology 2023Quote: ... Resulting plasmid DNA was transiently transfected into HEK Expi293-F cell line using the ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific). Six days after transfection ...
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bioRxiv - Bioengineering 2023Quote: HEK 293 cells were cultured at 37 °C and 5% CO2 using DMEM high glucose without phenol red (Gibco, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum ...
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bioRxiv - Bioengineering 2023Quote: HEK 293 cells were cultured at 37 °C and 5% CO2 using DMEM high glucose without phenol red (Gibco, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2024Quote: HEK-293 cells (ATCC, CRL-1573) were subcultured every 3-4 days in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, 41965-039) supplemented with 10% v/v fetal bovine serum (Sigma Aldrich ...
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bioRxiv - Biophysics 2021Quote: Wild-type SCN5A (Q1077del NaV1.5)16 was co-transfected with pEGFP-C1 into HEK-293 cells with Lipofectamine 3000 reagent (Thermo Fisher Scientific, Massachusetts, USA).
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bioRxiv - Pharmacology and Toxicology 2019Quote: HEK-293 cells or HEK-293 cells stably expressing PAR4-YFP were loaded with calcium sensitive dye (Fluo-4 NW, F36206, Life Technologies, Thermo Fisher) and aliquoted into cuvettes with Hanks buffered salt solution (HBSS containing Ca2+ and Mg2+) ...
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bioRxiv - Neuroscience 2020Quote: Destabilized EGFP reporter with or without 3’UTR of interest was transduced into HEK 293 cells to establish a stable reporter containing cell line with Blasticidin S HCl (Life Technologies, A11139-03) selection ...