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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for 2 Trimethylsilyl ethynyl toluene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Cell proliferation in response to GNE-3511 treatment was assessed by 5-ethynyl-2′-deoxyuridine (EdU) incorporation using the Click-iT EdU Alexa Fluor 488 cell proliferation assay kit (Invitrogen, Carlsbad, CA). CAL33 cells were seeded in µ-Plate 24 Well plates (Ibidi GmbH ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were exposed to the medium supplemented with 100 nM HaloTag TMR ligand and 10 µM 5-ethynyl-2′-deoxyuridine (EdU) (C10337; Thermo Fisher Scientific) for 30 min immediately after washing out 7BRO ...
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bioRxiv - Cell Biology 2024Quote: The proliferation rate was assessed by quantifying 5-ethynyl-2’-deoxyuridine (EdU) cellular incorporation using the Click-iTTM EdU Alexa FluorTM 488 Imaging kit (Thermo Fisher Scientific). A number of 4.0x104 ECFCs were plated on collagen-coated flasks 1.86 cm2 surface area ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with 0.2 mM Ethynyl Uridine (EU; Thermo Fisher) for 8 hours and harvested in 1 ml TRIzol reagent (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2021Quote: A dam at E16.5 in gestation was given an intraperitoneal (IP) injection with EdU (5-ethynyl-2’-deoxyuridine) from the Click-iTTM EdU Alexa FluorTM 647 Imaging Kit (Invitrogen, Carlsbad, CA, USA) at a dosage of 0.25mg /10g body weight ...
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bioRxiv - Developmental Biology 2021Quote: Embryos and larvae from 9 hpf to 4 wpf were incubated in 3 µM 5-Ethynyl-2′-deoxyuridine (EdU; ThermoFisher Scientific, cat#: C10639) in FSW for 15-30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... the nascently synthesized DNA was labeled with 5-ethynyl-2′-deoxyuridine (EdU) and Alexa Fluor 488 azide through Click reaction using a Click-iT EdU Imaging kit (Life Technologies, cat# C10337) and then cells were stained with propidium iodide (PI ...
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bioRxiv - Developmental Biology 2020Quote: Cumulative curve of the thymidine analog 5-Ethynyl-2’-deoxyUridine (EdU) incorporation is performed using Click-iT™ Plus EdU Alexa Fluor™ 647 Imaging Kit (ThermoFisher; C10640). Briefly ...
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bioRxiv - Neuroscience 2020Quote: Proliferation of pericytes was determined by incorporation of the thymine analogue 5-ethynyl-2’-deoxyuridine (EdU) with the Click-iT®Assay Kit (Life Technologies C10340) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 5×103 cells/well were plated 5 days post infection on viewPlate-96black and the next day the cells were treated with 10 µM 5-ethynyl-2’-deoxyuridine EdU (Thermo Fisher, Eugene, OR) for 4 h and fixed in 4% paraformaldehyde in PBS ...
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bioRxiv - Microbiology 2023Quote: ... malayi cell suspensions were assessed for proliferative activity using the thymidine analog EdU (5-ethynyl-2’-deoxyuridine) and the Click-iTTM EdU Cell Proliferation Kit for Imaging with the Alexa FluorTM 488 dye (Thermo Fisher, Waltham, MA). Cells dissociated from B ...
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bioRxiv - Developmental Biology 2023Quote: Cell cycle changes were additionally assessed using the 5-ethynyl-2’-deoxyuridine (EdU) assay using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (ThermoFisher, C10418). HUH7.5 cells were incubated at 37 °C for 2 hours with 10 µM EdU using the Click-iT EdU Assay Kit (Thermofisher ...
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bioRxiv - Cell Biology 2020Quote: ... and pulse-labelled with 20 µM 5-ethynyl deoxy-uridine (EdU; Invitrogen) and 1 µM FuDR (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... and treated with 1 mM 5-ethynyl uridine (ThermoFisher, Catalog No. C10330) for 20 min ...
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bioRxiv - Cell Biology 2024Quote: ... 200 mg of 5-ethynyl-20-deoxyuridine (EdU; Thermo Fisher Scientific, #C10337) dissolved in PBS were injected intraperitoneally 2 to 3 hours before euthanasia ...
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bioRxiv - Immunology 2022Quote: ... Slides were deparaffinized and washed using two rounds of toluene (T324-1; Thermo Fisher Scientific, Waltham, MA) (5 min each ...
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bioRxiv - Immunology 2022Quote: We estimated hemocyte differentiation by visualizing and quantifying the synthesis of new DNA in vivo based on 5-ethynyl-2′-deoxyuridine (EdU) incorporation and subsequent detection using the Click-iT EdU Alexa Fluor 647 kit (Invitrogen, Grand Island, NY, USA) as previously described (36,37) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell proliferation was also assessed by 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay using Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: ... 5-ethynyl uridine (EU, Click-iT™ Nascent RNA Capture Kit, ThermoFisher Scientific) was added to the cell culture media at the time of the stimulation (final concentration 0.4mM) ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were cultured in 15cm plates and 0.1mM 5-ethynyl uridine (ThermoFisher Scientific) was incubated with cells after dox-induction for 16-hrs followed by a washout and then fixed at 48-hrs ...
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bioRxiv - Developmental Biology 2024Quote: ... pregnant female mice were intraperitoneally injected with EdU (5-ethynyl-20-deoxyuridine, Invitrogen) two hours prior to dissection for embryos ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 μl of N-methyl-N-trimethylsilyl trifluoroacetamide (MSTFA, Pierce) with 1% trimethylchlorosilane (1% TMCS, Thermo Scientific) was added to react for 30 min in 60°C ...
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bioRxiv - Plant Biology 2020Quote: Young seedlings were incubated in 20 μM 5-ethynyl-2’-deoxyuridine (EdU) (Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit, ThermoFisher Scientific/Invitrogen, Waltham, Massachusetts, USA) made in ddH2O for 30 min at 24 °C ...
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bioRxiv - Plant Biology 2021Quote: ... 100 µL of N-Methyl-N-(trimethylsilyl)trifluoroacetamide + 1% trimethylchlorosilane (MSTFA + 1% TMCS, Fisher Scientific, Ottawa, ON, Canada) was added and vials were incubated at 60 °C for an additional hour ...
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bioRxiv - Plant Biology 2024Quote: ... resuspended in dichloromethane and derivatized using N-Methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA) containing 1% trimethylchlorosilane (TMCS; Thermo Scientific). GC-MS was performed using an Agilent (Santa Clara ...
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bioRxiv - Genomics 2020Quote: Cells treated with transcriptional inhibitors were labelled with 1mM ethynyl-uridine (EU, ThermoFisher Scientific E10345) for 1 hour prior to fixation/permeabilization (0.5% paraformaldehyde ...
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bioRxiv - Cell Biology 2024Quote: ... Two mg of 5-ethynyl uridine (EU, Click-iT™ Nascent RNA Capture Kit, Invitrogen) per mouse were injected at 24 days post-partum (dpp ...
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bioRxiv - Molecular Biology 2023Quote: ... after a short incubation (30 minutes) with EU (5-ethynyl uridine, Thermo Fisher Scientific, E10342) and in the presence or absence of a MYC inhibitor (64 µM ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5-ethynyl uridine (EU, from the Click-iT Nascent RNA Capture Kit, Thermo Fisher) was added for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... cells were pulsed with 20 μM EdU (5-ethynyl-29-deoxyuridine, Life Technologies, Cat. #A10044) a nucleoside analog of thymidine ...
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bioRxiv - Systems Biology 2021Quote: ... A total of 90 μL of N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) + 1% trimethylchlorosilane (TMCS) (Thermo Scientific, Lafayette, CO, U.S.A.) was then added to the samples and shaken at 1400 rpm for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... sections were dehydrated in progressive ethanol concentrations and clarified in Toluene before mounting using Cytoseal-60 (Thermo Fisher Scientific, Waltham, MA). Slides were cured overnight in dark at RT before the imaging.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Solvents for lipid extraction and liquid chromatography (toluene, hexanes, isopropanol, methanol, water, and acetonitrile) were purchased from Fisher Scientific (Waltham, MA). Eluent additives for LC-MS (formic acid or NH4Ac ...
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bioRxiv - Biochemistry 2022Quote: ... Each dried aliquot of the non-polar layer was reconstituted in 50 μl of methanol: toluene (9:1, v/v) (Fisher Scientific) and transferred to 2 mL LC-MS amber vials with 300ul glass inserts ...
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bioRxiv - Molecular Biology 2023Quote: All samples were labeled with medium containing 5-ethynyl-20-deoxyuridine (EdU; Thermo Fisher scientific, E10187) at a final concentration of 20 μM for 11 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in medium containing 200 µM of 5-Ethynyl Uridine (Thermo Fisher Scientific, E10345) and after 3 h ...
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bioRxiv - Physiology 2020Quote: ... Samples were then derivatized with 25 µL of N,O-bis (Trimethylsilyl)trifluoroacetamide with Trimethylchlorosilane (BSTFA with 1% TMCS, Thermo Scientific) for 1h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... and sterols derivatized in 50 μL N,O-bis(trimethylsilyl) trifluoroacetamide with 1% trimethylchlorosilane (BSTFA + 1% TMCS, Thermo Fisher TS-38831) at 60°C for 1 h ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were derivatized in 50 μL of 1:1 N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA; Restek Corporation) and pyridine (Acros Organics) and incubated (70°C for 45 min.) ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then derivatised with 25 µL of N,O-bis (trimethylsilyl)trifluoroacetamide with trimethylchlorosilane (BSTFA with 1% TMCS, Thermo Scientific) for 1h at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: The newly synthesized RNA in OSC cells was labeled with 5-ethynyl-uridine (EU) (Life Technologies, #E10345) as described (61) ...
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bioRxiv - Cell Biology 2024Quote: ... 30 minutes prior to fixing cells were incubated with 1 µM 5-Ethynyl Uridine (EU) (Invitrogen #E10345), except for the unlabeled control ...
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bioRxiv - Cell Biology 2024Quote: ... Metabolites were further derivatized by adding 18 μl of N-methyl-N-(trimethylsilyl) trifluoroacetamide with or without 1% TMCS (Thermo Fisher Scientific) and 20 μl ethyl acetate (Sigma ...
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bioRxiv - Neuroscience 2024Quote: We derivatized the dried polar and protein samples using methoxyamine HCl (20 mg/mL) in pyridine and N-methyl-trimethylsilyl-trifluoroacetamide (MSTFA; Thermo Fisher Scientific). For the polar fraction ...
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bioRxiv - Neuroscience 2024Quote: A 5-ethynyl-uridine (EdU)-labeling experiment was conducted using the Click-iT EdU Imaging Kits by Invitrogen, adhering to the provided manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2020Quote: ... the samples were centrifuged before receiving 50 µL of N-Methyl-M (trimethylsilyl) trifluoroacetamide (MSTFA) + 1% trimethylchlorosilane (TMCS) (ThermoFisher Scientific, Waltham, MA, USA), briefly vortexed and incubated at 60 °C for 40 min ...
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bioRxiv - Systems Biology 2021Quote: ... the samples were centrifuged before receiving 50 μL of N-Methyl-M (trimethylsilyl) trifluoroacetamide (MSTFA) + 1 % trimethylchlorosilane (TMCS) (ThermoFisher Scientific, Waltham, MA, USA), briefly vortexed and incubated at 60 °C for 40 min ...
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bioRxiv - Synthetic Biology 2020Quote: ... Dried samples were dissolved in 250 µl of silylation-grade acetonitrile followed by addition of 250 µl of N-methyl-N- (trimethylsilyl)trifluoroacetamide (MSTFA) with 1% trimethylchlorosilane (TMCS) (Thermo Scientific, Bellefonte, PA) and heated for 1 hr at 70 °C to generate trimethylsilyl derivatives ...
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bioRxiv - Plant Biology 2022Quote: ... The sample was dried under a stream of air and resuspended in 50μL of N-methyl-N-(trimethylsilyl) trifuoroacetamide (MSTFA) (Fisher scientific, Waltham, MA, USA), votexed to mix for 20 sec ...
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bioRxiv - Systems Biology 2023Quote: ... Val-Tyr-Val, methoxyamine hydrochloride (MeOX), N-methyl-N-(trimethylsilyl)-trifluoroactamide (MSTFA), and pyridine (Anhydrous, 99.8%) were all purchased from Fisher Scientific (Hampton, NH, USA). Fatty acid methyl esters (FAMEs ...