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Citations for Thermo Fisher :
2401 - 2450 of 9091 citations for Recombinant Human SELE Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... and 10% FCS in culture dishes (100 mm with vents; Fisher Scientific UK Ltd) at 37°C in a humidified incubator at 5% CO2 ...
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bioRxiv - Microbiology 2019Quote: ... containing 10% FCS and 100 units/ml penicillin-streptomycin solution (Pen/Strep, Gibco, Belgium). Virus culture and cytopathic effect-based virus neutralization assays (CPENT ...
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bioRxiv - Cancer Biology 2019Quote: ... washed and resuspended in FC buffer with goat-anti-mouse Alexa488 (Invitrogen; 1:400) for an additional 15 minutes ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Biowaste) and 1% penicillin/streptomycin (Life Technologies).
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bioRxiv - Synthetic Biology 2020Quote: ... Absorbance at 405 nm was recorded with Multiskan FC microplate photometer (Thermo Fisher Scientific). All shaking steps were conducted in Titramax 1000 (Heidolph Instruments ...
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bioRxiv - Cell Biology 2021Quote: ... TβRII-Fc (124 kDa) and murine mAb 1D11 (150 kDa) (Thermo Fisher, Waltham, MA) were each captured on individual flow cells to 100-140 RU ...
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bioRxiv - Immunology 2020Quote: ... 2 mM L-Glutamine and 50 µm 2-Mercaptoethanol) with 10% FCS (all Gibco). Colon and caecal tissue were digested in an enzyme cocktail of 0.45 mg/ml collagenase V (Sigma-Aldrich) ...
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bioRxiv - Physiology 2019Quote: ... The ELISA was read using a filter-based accuSkan FC micro photometer (Fisher Scientific). The limits of sensitivity were <0.188 ng/ml and < 0.375 ng/ml for MUC5AC and MUC5B ...
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bioRxiv - Microbiology 2021Quote: ... to remove clumps and 10 ml RPMI supplemented with 10% FCS (Thermo Fisher Scientific) was added ...
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bioRxiv - Cell Biology 2021Quote: ... Digestion was blocked by transferring the halves to a dish containing FCS (Gibco, USA) and the epidermis peeled off the dermis ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were blocked prior to staining using anti-CD16/32 (93, ThermoFisher Scientific). Where >1 brilliant violet or brilliant UV dye was used concurrently ...
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bioRxiv - Immunology 2022Quote: ... followed by Fc-receptor blockade with anti-CD16/CD32 (Thermo Fisher #14-0161-85), and then stained for 30 min on ice with the following antibodies in flow cytometry staining buffer (FACS) ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% v/v foetal calf serum (FCS) (Thermo Fisher Scientific, Waltham, MA) and 1% v/v penicillin/ streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... to remove clumps and 10 ml RPMI supplemented with 10% FCS (Thermo Fisher Scientific) was added ...
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bioRxiv - Microbiology 2023Quote: Cells were then frozen in fetal calf serum (FCS) (ThermoFisher Scientific, Cat. No. 10500064) 10% DMSO (v/v ...
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bioRxiv - Immunology 2022Quote: ... Optical density (OD) was measured using a Multiskan™ FC (Thermo Fisher-Scientific UK) plate reader at 405 nm and S1-specific IgG titers were interpolated from the IgG standard curve using 4PL regression curve-fitting on GraphPad Prism 9.
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bioRxiv - Microbiology 2022Quote: ... supplemented with 10% v/v foetal calf serum (FCS) (Thermo Fisher Scientific, Waltham, MA) and 1% v/v penicillin/ streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... Medium was removed and cells were transferred in FCS-free essential medium OptiMEM (Gibco) 2 h before transfection ...
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bioRxiv - Microbiology 2024Quote: ... 10% (v/v) heat inactivated fetal calf serum (FCS, Hyclone, Thermo Scientific, Rockford, IL), 100 U/mL penicillin ...
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bioRxiv - Synthetic Biology 2023Quote: ... Fc-PRL-13 was purified with Protein A agarose resin (Thermo Fisher Scientific; 20334) according to the manufacturer’s instructions with the exception that all buffers were supplemented with 1tab/50mL of cOmplete Protease Inhibitor Cocktail and 10uM Pepstatin A ...
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bioRxiv - Developmental Biology 2023Quote: ... supplemented with 10% (v/v) heat-inactivated FCS (Invitrogen, 10270-106, batch number 41F8126K), 2,000 units ml−1 LIF (Millipore ...
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bioRxiv - Synthetic Biology 2023Quote: ... according to the manufacturer’s instructions using a Multiskan™ FC microplate photometer (Thermo Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... Detection was performed with secondary goat anti-mouse IgG Fc HRP antibody (Invitrogen, A16084) and WesternBright Sirius chemiluminescent HRP conjugate (advansta ...
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bioRxiv - Neuroscience 2023Quote: ... single cell suspensions were incubated with Fc Block CD16/32 (Invitrogen; 14-0161-85). The cells were then incubated for 30 minutes with the surface antibodies and Live/Dead Ghost Dye Violet 510 (Tonbow Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS, Gibco Cell Culture, 10270-106), 0.5% (v/v ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions were incubated with Fc Block CD16/32 (Invitrogen, 14-0161-85). The cells were then incubated with a 1:200 dilution of antibody for surface stains and A Live/Dead Ghost Dye Violet 510 (Tonbo Biosciences ...
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bioRxiv - Immunology 2023Quote: ... and cryopreserved at −150°C in 40% heat-inactivated fetal bovine serum (FCS, Gibco), 50% RPMI-1640 (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with anti-CD16/CD32 (diluted 1:50, Thermo Fisher Scientific) and cells were stained with cell surface antigens ...
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bioRxiv - Cell Biology 2023Quote: ... After Fc receptor blockage using an antibody for CD16/32 (14-0161-85, Invitrogen), stainings were performed in FACS buffer (1 % BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10 % fetal calf serum (FCS, Gibco, ThermoFisher Scientific, Hanover Park, IL, USA). In order to induce the Tripz-construct cells were treated with a final concentration of 5 µg/ml doxycycline hyclate (DOX) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and Fc fusions were purified with Protein A agarose resin (Thermo Fisher Scientific; 20334) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Fc glycans were released and labelled using the GlycanAssure ATPS kit (Thermo Fisher Scientific) then separated using 3500xL Genetic Analyzer (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... Siglec-Fc constructs were expressed according to the Expi293F manufacturer protocol (Thermo Fisher Scientific) and purified by manual gravity column (BioRad ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 10% fetal calf serum (FCS, Biowaste) and 1% penicillin/streptomycin (Gibco, 15140).
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bioRxiv - Immunology 2021Quote: ... IL-2 Human Uncoated ELISA kit and IFNγ Human Uncoated ELISA kit (both from Invitrogen) and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... rat anti-human (16G6) and rabbit anti-human polyclonal CD49a were all purchased from ThermoFisher. For collagen measurements 10 mg of allograft tissue/sample was analyzed with a Hydroxyproline Assay Kit (Sigma ...
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bioRxiv - Pathology 2022Quote: ... Aβ42 human ultrasensitive ELISA Kit and Tau (phospho) [pT231] human ELISA Kit from Thermo Fisher, and sAPPα and sAPPβ ELISA Kit from Mybiosource ...
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bioRxiv - Genomics 2021Quote: ... surviving human leucocytes in thawed samples were removed using anti-human CD45 DynaBeads (ThermoFisher Scientific). The resulting parasite pellet was washed to remove soluble human DNA (hDNA) ...
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bioRxiv - Genomics 2022Quote: Human total RNA was extracted from THP-1 human cells using TRIzol (ThermoFisher, cat# 15596026) as per manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... human kinome panel (Cat # 4475388) and human stem cell openarray panel (Cat # 4475390, ThermoFisher, USA). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... lipid membranes were incubated with a 6x-His tag polyclonal antibody HRP conjugate (MA1-21315, ThermoFisher) in blocking buffer for one hour at room temperature followed by three washes with wash buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse SGK1.1 (wild type and constitutively active mutant S515D) cloned into pcDNA3.1/V5-His-TOPO (Invitrogen) were kind gifts from Dr ...
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bioRxiv - Genomics 2019Quote: ... Hi-C DNA bound to capture RNA was isolated using a DynaMag-2 magnet (Life Technologies). Washes (15 min in 500 μl wash buffer I at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Completed reactions were ethanol precipitated and re-suspended in deionized formamide (Hi-Di formamide, Thermo Fisher) with the G-ladders ...
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bioRxiv - Genetics 2019Quote: ... The His-ChpBK fusion protein was purified from clarified lysates using a Ni-NTA beads (Invitrogen). Protein purity was determined by SDS-PAGE analysis and samples were quantified using the Bio-Rad protein assay and stored at −80 □.
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bioRxiv - Biochemistry 2020Quote: ... optiMEM (1X) reduced serum medium and heat-inactivated fetal bovine serum (HI-FBS) were from Gibco chemicals ...
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bioRxiv - Neuroscience 2021Quote: ... and labeled with 6x-His Tag Antibody coupled to Alexa 555 (Thermo Fisher, MA1-21315-A555). Coverslips were mounted on slides (Aqua-Poly/Mount ...
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bioRxiv - Plant Biology 2021Quote: ... BgtCDA1 coding sequences with a 6XHIS tag were amplified and ligated into pPIC9K-HIS vector (Invitrogen), and then transformated into Pichia pastoris strain GS115 (Invitrogen) ...
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An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brainbioRxiv - Molecular Biology 2022Quote: ... All cloning was performed using a pcDNA 3.1-V5/His TOPO-TA cloning kit (Invitrogen K480001) per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... This mix was added to 50 µl pre-washed His-Pur magnetic Nickel beads (Thermofisher Scientific) and incubated for 1 h at 4 °C on a rotor ...