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Citations for Thermo Fisher :
2401 - 2450 of 10000+ citations for 7 BROMO 2 3 4 5 TETRAHYDRO 1H BENZO E 1 4 DIAZEPINE 2 CARBOXYLIC ACID METHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... samples were incubated at 4°C covered from light for 30 minutes with 2 μl/ml Fixable Viability Dye eFluor506 (Thermo Fisher) while in PBS and then resuspended in rat anti-mouse CD16/32 (Fc block ...
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bioRxiv - Cancer Biology 2021Quote: Cells were seeded at a density between 2 × 104 and 4 × 104 cells per well in Nunc Lab-Tek II CC2 chamber slides (Thermo Scientific) based on their proliferation rate and left to establish for 48hrs at 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We extracted RNA from sets of 8 adult males (2~4 day old) in triplicate from each RNAi cross using TRIzol (Catalog# 15596-026, Invitrogen, USA), treated ~2 μg RNA with RNase-free DNase I (Catalog# M0303S NEW ENGLAND Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... ISP2 and ISP4 (International Streptomyces Project medium 2 and 4; premixed Difco™ ISP2 dehydrated medium (Fisher Scientific, #DF0770-17-9) and premixed Difco™ ISP4 dehydrated medium (Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Fisher Scientific, Waltham, MA, USA) equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Immunology 2021Quote: ... equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm, 4 μm, Thermo Fisher Scientific, Waltham, MA, USA). The column temperature was held at 30°C ...
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bioRxiv - Immunology 2021Quote: ... samples were incubated at 4°C covered from light for 30 minutes with 2 ul/ml Fixable Viability Dye eFluor506 (Thermo Fisher) while in FACS buffer (PBS with 2% newborn calf serum ...
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bioRxiv - Immunology 2020Quote: ... Conjugated VIPR particles were centrifuged at 14,500 xg for 3 minutes at 4°C and washed three times in PBS while the beads were immobilized in an Invitrogen DynaMag-2 magnet (Thermo Scientific). VIPR particles were resuspended in sterile PBS at a concentration of 5×108 particles per ml and stored at 4°C.
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bioRxiv - Microbiology 2021Quote: Fifty microliters containing 3.5 x 106 PFU/ml SARS-CoV-2 viral stock was placed on one well of a 4-well chambered glass slide (Nunc, Sigma) (Fig 2) ...
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bioRxiv - Cell Biology 2021Quote: ... Cultured colonies from organoids were fixed with 4% PFA for 2 hrs at room temperature followed by immobilisation with Histogel (Thermo Scientific) for paraffin embedding ...
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bioRxiv - Developmental Biology 2022Quote: ... tissue culture-treated plates and passaged every 2-4 days at approximately 70 percent confluency using Try-pLE Express Enzyme (GIBCO, #12605010). The absence of Mycoplasma contamination in cells was regularly tested.
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bioRxiv - Genetics 2022Quote: cDNA was generated from 2 control iPSC clones and 4 frameshift iPSC clones with the Superscript III kit (Thermofisher 18080-051) following manufacturer’s recommendations using 1ug of RNA per sample and random hexamer primers ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then dipped in the secondary antibody-containing buffer with the nuclear dye 4′,6-diamidino-2-phenylindole (DAPI, Cat# D1306, Life Technologies-Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclear staining of glass-mounted brain sections was performed by 10 min incubation with PBS containing 2.5% DAPI (4’,6-diamidino-2-phenylindole, Thermo Fisher Scientific). Sections were embedded in mounting medium containing 1,4-diazabicyclooctane (DABCO ...
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bioRxiv - Neuroscience 2022Quote: ... Images for the case of PD and case 2 of PDD were acquired using a Falcon-4 detector (Thermo Fisher Scientific). Images for case 1 of PDD and case 1 of DLB were acquired using a Falcon-4i detector (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... 2 x10^6 in 6 cm dishes or 4 x10^6 in 10 cm dishes (Nunc Edge plates, Thermo Fisher Scientific) in conditioned NB or NB-Plus ...
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bioRxiv - Neuroscience 2022Quote: ... 2 x10^6 in 6 cm dishes or 4 x10^6 in 10 cm dishes (Nunc Edge plates, Thermo Fisher Scientific) in conditioned NB or NB-Plus ...
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bioRxiv - Biophysics 2024Quote: ... in PBS and incubated over night at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech ...
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bioRxiv - Cancer Biology 2024Quote: ... the tissue specimens were stained with 4′,6-diamidino-2-phenylindole (DAPI) for 10 min and mounted in VECTASHIELD Vibrance Antifade Mounting Medium (ThermoFisher Scientific). Akoya Biosciences’ PhenoImager HT (formerly known as Vectra Polaris Automated Quantitative Pathology Imaging System ...
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bioRxiv - Cell Biology 2023Quote: ... DIV5 mouse hippocampal neurons were incubated with 20 nM Reelin or Mock medium for 2 h after 4 h of incubation in depletion medium (only neurobasal medium (Gibco, Thermo Fisher) plus 1% GlutaMAX) ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... Anion exchange chromatography was conducted on a Dionex IonPac AS11-HC column (2 mm X 250 mm, 4 µm particle size, Thermo Scientific) equipped with a Dionex IonPac AG11-HC guard column (2 mm X 50 mm ...
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bioRxiv - Immunology 2023Quote: ... Nuclei were detected and cover slips were attached using 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) containing Prolong Diamond Anti-Fade (Life Technologies). Slides were imaged on a Keyence BX-X810 microscope.
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bioRxiv - Cell Biology 2023Quote: ... A final wash with PBS was performed before mounting the cells in ProLong® Gold Antifade Mounting medium with DAPI (4’,6-diamidine-2-phenylindole)(Invitrogen).
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bioRxiv - Molecular Biology 2023Quote: ... After 3 washes cells were stained with a combination of Alexa Fluor secondary antibodies and 4′,6-Diamidino-2-Phenylindole (DAPI; Molecular Probes) for 1 h at room temperature in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... and ProLong™ Gold antifade reagent with 4′,6-diamidino-2-phenylindole (DAPI) (P36941) or without (P36930) was purchased from Invitrogen.
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bioRxiv - Molecular Biology 2023Quote: ... B cells were harvested 72h after activation and 4 x 107 cells were cross-linked by using 2 mM disuccinimidyl glutarate (ThermoFisher 20593) in PBS for 45 min and 1% formaldehyde for 5 min (Thermo Scientific 28908) ...
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bioRxiv - Bioengineering 2023Quote: ... the hydrogel is first activated with sulfosuccinimidyl 6-(4′-azido-2′-nitrophenylamino) hexanoate (Sulfo-SANPAH, Pierce, Thermo Fisher Scientific, Waltham, MA). Here the remaining washing buffer around the gel edge is removed to ensure the coating solution can be held on top of the gel ...
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bioRxiv - Cell Biology 2023Quote: ... and coverslips were mounted using Prolong Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole (DAPI) (Life Technologies, Carlsbad, CA, USA). Cells were viewed using an Olympus IX81 or Olympus BX53 epifluorescence microscope ...
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bioRxiv - Neuroscience 2023Quote: ... and loaded for 2 h at 4°C on a bi-directional rotator with 2 mM Ca2+ medium containing drugs 30 μM pancuronium (Fisher Scientific), or 38.9 μM curare (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µL was loaded onto an IonPac AS-11 HC strong anion-exchange analytical column (2 mm × 250 mm, 4 µm particle diameter, Thermo Scientific) with AG-11 HC guard column (2 mm × 50 mm ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were then mixed with 2 x of the NuPAGE™ LDS Sample Buffer 4 x (Novex, Life Technologies, USA) with addition of 10% β-mercapto-ethanol and incubated for 5 min at 98 °C ...
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bioRxiv - Cell Biology 2023Quote: ... additionally this was equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm, 4 μm, Thermo Fisher Scientific). The column temperature was held at 30°C and the auto sampler was set to a temperature of 6°C ...
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bioRxiv - Neuroscience 2023Quote: ... Three 5 min washes with PBS preceded a 2-hour incubation at room temperature with 4 μg/mL Alexa Fluor 647-conjugated secondary antibody (ThermoFisher A21244) prepared in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining 50 μL were incubated at 4°C for 2 h with 40 μg of pre-equilibrated HisPur Ni–NTA magnetic beads (Thermo Scientific) in a final volume of 200 μL ...
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bioRxiv - Physiology 2024Quote: ... Cells were loaded at 37°C for 60 minutes with 4 μM Fura-2-AM in the presence of 1X Power Load permeabilizing reagent (Thermo Fisher). Injection of AngII (10-8M) ...
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bioRxiv - Cancer Biology 2024Quote: ... RIPA Lysis and Extraction Buffer (CAT NO. 89901), and DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) (CAT NO. D3571) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... coverslips were mounted on glass slides using ProLong Gold antifade (4′,6-diamidino-2-phenylindole) DAPI to counterstain the nuclei (Thermofisher, #P36935).
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bioRxiv - Neuroscience 2024Quote: The pipette solution (EGTA omitted) was supplemented with 100 μM calcium-sensitive dye Fluo-4 or Fura-2 (Thermo Fisher Scientific) and 50 μM calcium-insensitive dye Alexa Fluor 568 (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... The remaining 45 μL were added to 2 μL of settled anti-Flag magnetic beads (4 μL of 50% slurry) (Thermo Fisher) that had been equilibrated in Wash Buffer ...
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bioRxiv - Cell Biology 2023Quote: Tubulin was labelled with (5(6)-TAMRA Succinimidyl Ester (Invitrogen, C1171) for fluorescence microscopy assays according to published methods (Consolati et al ...
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bioRxiv - Cell Biology 2022Quote: ... 200µL 5-Ethynyl-2’-deoxyuridine (EdU, Molecular probes; 3g/L) was injected intraperitoneally in pregnant females 2 hours before embryo isolation.
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bioRxiv - Immunology 2019Quote: ... 2 µL of the primer mix (5 mM dNTP [Invitrogen] ...
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bioRxiv - Developmental Biology 2020Quote: ... and 5.5 x 10-5 mol/L 2-mercaptoethanol (Gibco). For the endothelial potential assay ...
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bioRxiv - Cell Biology 2020Quote: ... For 5-ethynyl-2’-deoxyuridine (EdU) incorporation assays (Life Technologies), 10μM EdU was included in culture for two hours ...
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bioRxiv - Developmental Biology 2021Quote: ... 5-ethynyl-2′-deoxyuridine (EdU) (Thermo Fisher Scientific, cat. #E10415) was diluted 2.5 mg/ mL in sterile PBS and injected intraperitoneally on days 3 and 4 post-injury at a dose of 10 μL/ g body weight ...
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bioRxiv - Neuroscience 2020Quote: ... 5-ethynyl-2’-deoxyuridine (EdU; Thermo Fisher Scientific, Waltham, MA) was either injected i.p ...
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bioRxiv - Cancer Biology 2022Quote: ... or 2 - 5 μg/ml Puromycin Dihydrochloride (Thermo Fisher, # A1113803) until all negative control cells were dead ...
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bioRxiv - Microbiology 2022Quote: ... or 0.1 mM 5-ethynyl-2’-deoxyuridine (EdU; Life Technologies) was added for the time indicated in the text.
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bioRxiv - Cancer Biology 2022Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher Scientific, Barcelona, Spain) was added for 48h ...
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bioRxiv - Biophysics 2020Quote: ... while GRN-5 was expressed in Origami 2 DE3 (Invitrogen) as fusion constructs with a thioredoxin-A and hexa-histidine tag (TrxA-Hisx6-GRN) ...