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Citations for Thermo Fisher :
2401 - 2450 of 10000+ citations for 6 Methoxy 2 3 4 9 tetrahydro 1H b carbolin 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Vero cells were infected with passage 9 virus and overlaid with 1% low melting agarose (Invitrogen) in MEM ...
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bioRxiv - Molecular Biology 2023Quote: ... were grown in LHC-9+ 1% penicillin G (Pen) and streptomycin (Strep) (serum-free) medium (Gibco) following established protocols under sterile conditions (12) ...
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bioRxiv - Cell Biology 2021Quote: ... diluted cell supernatant was preabsorbed for one hour at 4°C with protein A/G magnetic beads (Invitrogen). The supernatant fraction was incubated overnight at 4°C with an appropriate antibody and protein A/G magnetic beads were blocked with 4% BSA ...
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bioRxiv - Molecular Biology 2020Quote: One hundred micrograms of mitochondrial proteins were separated on 4-16% (w/v) polyacrylamide Native PAGE gels (Invitrogen) and then either transferred to PVDF membranes or subjected to in-gel activity staining as in previously described in (25) ...
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bioRxiv - Microbiology 2022Quote: ... A total of 4 μg of RNA was treated with one unit of DNase I Amplification Grade (Invitrogen) to remove potential contamination with genomic DNA ...
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bioRxiv - Microbiology 2022Quote: ... Eluted protein complexes were separated by one-dimensional 4-12% NuPage Novex Bis-Tris Gel (Invitrogen, Cat. NP0321BOX) and stained using the Colloidal Blue Staining Kit (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... cells were live-dead stained with 2 µM calcein acetoxymethyl and 4 µM ethidium homodimer-1 (#L3224, Thermo Fisher) and imaged using fluorescent microscopy to compare cell density and viability between each silicone substrate ...
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bioRxiv - Biochemistry 2021Quote: ... 2 µg PAX2 and 4 µg shRNA in pLKO.1 backbone using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... thinly sliced (1-2 mm) tissue samples were fixed overnight at 4°C in neutral-buffered formalin (Fisher Scientific) with PhosSTOP added (Roche) ...
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bioRxiv - Bioengineering 2024Quote: ... PEGαMA hydrogels were made by dissolving the PEGαMA in pH 8.4 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Life Technologies) at 12.5-15.5 weight percent (wt%) ...
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bioRxiv - Neuroscience 2022Quote: ... was dissolved in 0.9% sterile Saline and pH was adjusted to 7.4 with 20-30 µl 1 N NaOH (#SS266-1, Fisher Scientific) according to Tocris Bioscience’s guidelines ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was isolated from 9 µl lysate and 1 µl of ERCC 1:1000 Spike in control (Thermo Fisher Scientific) per sample using RNA SPRI beads (Agencourt ...
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bioRxiv - Cell Biology 2019Quote: ... the dishes were coated with 1 ml of gelatin in PBS (1: 9 of 0.1% fluorescein isothiocyante (FITC)-gelatin (# G13186, Invitrogen, MA): 2% porcine gelatin) ...
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bioRxiv - Molecular Biology 2019Quote: Phage proteins were isolated from a purified phage lysate (10^9 pfu/ml) by a single methanol/chloroform extraction (1:1:0.75, v/v/v) (Acros Organics) and precipitated by addition of an equal volume of methanol (14,000 x g ...
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bioRxiv - Bioengineering 2023Quote: Passage 3 and passage 4 cells from 8 donors (4 male and 4 female) were expanded in T175 flasks (Thermo Fisher Scientific, Hampton, New Hampshire USA) and were cultured until passage 5 (p3-5 and p4-5 ...
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bioRxiv - Biochemistry 2020Quote: ... The transfection mixture for HEK293T cells was created by adding 9 μL Lipofectamine™ LTX Reagent with 3 μL PLUS™ Reagent (Thermo Scientific #15338100) and 3 μg of a plasmid to 500 μL Opti-MEM™ (Thermo Scientific #31985062) ...
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bioRxiv - Neuroscience 2022Quote: ... cells were loaded in microglia differentiation medium with 3 μM Fluo-4 AM and 3 μM Fura-Red AM (Molecular Probes) in the presence of Pluronic Acid F-127 (Molecular Probes ...
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bioRxiv - Cell Biology 2022Quote: ... Blocks of tissue of ~3×3×4 cm (depth×width×height) were cut and prepared for sectioning using a Vibrating Blade Microtome (Thermo Fisher) at a thickness of 500μm ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-Map2 a,b,c (MA5-12826; Thermo Fisher Scinetific;1:500), anti-Neuron Specific Enolase (NSE ...
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bioRxiv - Cell Biology 2022Quote: ... Selection was performed using 100 μg mL-1 hygromycin B (ThermoFisher Scientific) and 15 μg mL-1 blasticidine (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... B-cells were pre-stained with 1 µM CFSE (Thermo Fisher Scientific) for 10 min at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... 1:100 B-27 supplements without vitamin A (Thermo Fisher Scientific, 12587010), 1:200 GlutaMAX™ (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and Fos-B (anti-FosB antibody 1:500, rabbit, Ma5-15056, Invitrogen) immunofluorescence experiments and quantification of Fos-B expression are detailed in supplementary methods.
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bioRxiv - Neuroscience 2021Quote: ... growth factors and inhibitors included 1× B-27 supplement (Thermo Fisher Scientific), 0.5% penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: ... pH 7.5) containing 1% n-dodecyl-b-D-maltoside (DDM; Thermo Scientific). The resultant samples were resuspended in 1X Laemmli buffer containing 5% β-Mercaptoethanol (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were selected using 150 µg ml-1 hygromycin B (Thermo Scientific) and maintained in DMEM (Thermo Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... and 2.50 μg ml-1 Amphotericin B (Gibco-Thermo Fisher Scientific, 15290026). The extraction was performed for 24 hr at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2021Quote: ... and 2.50 μg ml-1 Amphotericin B (Gibco-Thermo Fisher Scientific, 15290026). The extraction was performed for 24 hr at 37°C and 5% CO2 ...
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bioRxiv - Physiology 2020Quote: ... The minimal substrate medium included 1% B-27 Supplement (Cat# 17504044, Gibco), 1 mM glutamine ...
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bioRxiv - Developmental Biology 2021Quote: ... 1/200 B-27 Supplement Minus Vitamin A (50x; Invitrogen; 12587-010); 1/100 MEM Non-Essential Amino Acids Solution (10 mM ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1% (v/v) penicillin-streptomycin-amphotericin B solution (Gibco, 15240-062). Hepatocytes were seeded on polystyrene tissue culture dishes of appropriate size pre-coated by collagen type I (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1× B-27 Supplement without vitamin A (Gibco, Carlsbad, CA, USA); and serially diluted and cultured as described previously [16] ...
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bioRxiv - Cancer Biology 2022Quote: ... B-27™ supplement (1:1000, Thermo Fisher Scientific, Waltham, MA; #17504044), penicillin-streptomycin (GenDEPOT ...
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bioRxiv - Neuroscience 2024Quote: ... 1:100 B-27 supplements without vitamin A (Thermo Fisher Scientific, 12587010), 1:200 GlutaMAX™ (Thermo Fisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... plates were washed 1 × with Neurobasal A with B-27 (Life Technologies) and 0.5 mM Glutamax and replaced with glial conditioned Neurobasal A medium containing B27 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then labelled for Methoxy-X04 (10 minutes at RT) or counterstained with DAPI and mounted with prolong gold anti-fade medium (Invitrogen, P36930). Images were acquired using a Zeiss LSM700 confocal microscope with the Zen 2010 Software package ...
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bioRxiv - Cell Biology 2021Quote: ... Daily media changes were performed up to day 9 when cells were fixed with 100% ice-cold Methanol (Fisher Scientific, cat # A454-4). Images were acquired on a Nikon Instruments Ti2 inverted fluorescence microscope ...
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bioRxiv - Cell Biology 2019Quote: ... The resulting PCR product was then inserted into the 5’ SpeI and 3’ EcoRI sites of the pMT/V5 His-B vector (Invitrogen) containing in-frame mTurquoise2 gene at the 5’ end ...
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A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: The HIF-1α-PAS-B domain was coated at 3 μg/ml onto 96-well microtiter plates (Thermo Fisher Scientific) and incubated overnight at 4 °C ...
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bioRxiv - Biophysics 2020Quote: ... DiIC18(1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindotri-carbocyanine Iodide) and the Alexa 488-labeled fragment B of cholera toxin were purchased from Thermo Fisher scientific (Waltham ...
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bioRxiv - Genetics 2022Quote: ... Hygromycin-resistant colonies emerging on the surface of the overlay after 3–5 days were excised and transferred to MEA amended with 100 µg/mL of hygromycin B (Invitrogen) (MEA+Hyg100) ...
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bioRxiv - Immunology 2021Quote: ... Qβ-VLPs were then re-packaged with B-type 1668 CpGs (5″-TCC ATG ACG TTC CTG ATG CT-3″) with phosphorothioate backbone purchased from (InvitroGen). The re-packaging was confirmed by 1% agarose gel stained with SYBR Safe dye for 30min at 90V ...
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bioRxiv - Neuroscience 2023Quote: ... over 75 min and from 55% to 100% solvent B in 3 min at a constant flow rate of 0.25 μl/min on UHPLC Easy-nLC 1000 (Thermo Scientific) where the LC system was connected to a 23-cm fused-silica emitter of 75 μm inner diameter (New Objective ...
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bioRxiv - Microbiology 2022Quote: ... anti-His×6 (mouse monoclonal, Invitrogen, 1:10,000-20,000), anti-OmpA (rabbit polyclonal ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA from whole retinas was extracted using TRIzol (6 retinas from 3 fish per sample) (Invitrogen). RNA was quantified using Nanodrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Reverse 5’-CGA AGG TGT GAC TTC CATG-3’) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). A standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
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bioRxiv - Immunology 2020Quote: ... plates were incubated with 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) substrate (ABTS, Thermo Fisher Scientific) for 15 min at RT shielded from light and absorbance was measured at optical density (OD ...
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bioRxiv - Neuroscience 2023Quote: ... 6 and 8 weeks of maturation were loaded with 2.5 µM Fluo-3-AM (Molecular Probes, #F1242) dissolved in differentiation medium for 30 min at 37 °C and at 5% CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... lungs were rinsed with antibiotics/antimycotics (10% P/S, 4% amphotericin-B [Sigma], 0.4% gentamicin [Gemini Bio] in PBS with Ca2+ and Mg2+ [Gibco]) followed by perfusion with 0.0035% Triton X-100 (American Bioanalytical) ...
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bioRxiv - Immunology 2023Quote: ... Raji B cells were labelled in serum-free medium with 10 μM 7-amino-4-chloromethylcoumarin (CMAC, Thermofisher, USA) for 1 hour at 37°C ...