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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for Recombinant Human WIBG Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Protein G Dynabeads (Invitrogen) coupled with anti-FLAG M2 antibody (F1804 ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein A Dynabeads (Invitrogen) were equilibrated with wash buffer (NP-40 omitting lysis buffer) ...
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bioRxiv - Microbiology 2024Quote: ... Protein G dynabeads (Thermofisher) were coated with rabbit α-myc antibody (Cell Signalling Technologies ...
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bioRxiv - Microbiology 2019Quote: Human IL-18 and TNFα were measured with the human IL-18 ELISA Kit (MBL, Woburn, Massachusetts) and human TNFα ELISA Kit (ThermoFisher, Waltham, Massachusetts) according to the manufacturers’ instructions using cell culture supernatant at a 1:5 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Human brain samples classified into healthy (n=47) and AD (n=32) were processed by array (Affymetrix Human Gene 1.0 ST Array)53 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Telomerase-immortalized human neonatal foreskin keratinocytes (N-TERTs) (21) were cultured in EpiLife medium containing human keratinocyte growth supplement (HKGS) (Thermo Fisher Scientific) and penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions of human PBMC were stimulated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher Scientific) as per manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... 0.01ug/ml soluble human anti-CD3 (R&D clone UCHT1) and 0.1 ug/ml soluble human anti-CD28 (Life Technologies; clone CD28.2) at a concentration of 1×106 cells/ml in RPMI-1640 (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... SH-SY5Y human neuroblastoma (ECACC) and RH-30 human rhabdomyosarcoma (kindly donated by Marc-David Ruepp) were maintained in DMEM (Thermo Fisher Scientific), supplemented with 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: Cell lines used in this study were THP-1 (human monocytic leukemia) cells, HeLa (Henrietta Lacks, human cervical carcinoma cells, CCL2) and HEK293T Flp-In T-REx 293 (Thermo Fisher Scientific). THP-1 cells were cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Immunology 2021Quote: ... Human primary CD8+ T Cells were negatively isolated from PBMCs using Dynabeads™ Untouched™ Human CD8 T Cells Kit (ThermoFisher Scientific) or Human CD8+ T Cell Isolation Kit (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... and the biotinylated secondary anti-human IgG (ref. B1140-1ML, MilliporeSigma, St. Louis, USA)] and the anti-human IgE (ref. A18803, Invitrogen, Waltham, USA) antibodies were added at 1:1250 and 1:100 dilutions ...
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bioRxiv - Cell Biology 2022Quote: ... were prepared from fresh human umbilical veins as previously described (Barbieri et al., 1981) and maintained in Human Endothelial SFM (Gibco™, Life Technologies Corporation ...
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bioRxiv - Cell Biology 2024Quote: ... Human kidney cells (HKC) and human embryonic kidney cells (HEK293T) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher Scientific, Cat. # 11965126). Mouse embryonic fibroblasts (MEF ...
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bioRxiv - Immunology 2024Quote: ... The cells were then washed in PBS and stained with a mix of secondary antibodies anti-human IgG-Fc-AF647 (1:600) and anti-human IgA-Alpha-Chain-AF488 (1:200) (Thermo Fisher Scientific) for 30 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... 0.01 µg/ml soluble human anti-CD3 (R & D; clone UCHT1) and 0.1 µg/ml soluble human anti-CD28 (Life Technologies; clone CD28.2) for 3 days before infection ...
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bioRxiv - Immunology 2023Quote: Human pluripotent stem cells (PSCs) were generated from anonymized human PBMCs based on published protocols115 using Sendai viral vectors (Thermo Fisher, A16517) driving high expression of pluripotency markers NANOG and OCT4 ...
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bioRxiv - Molecular Biology 2023Quote: ... All hFRG mice reported in this study were engrafted with human and non-human primate hepatocytes from the same donors (Caucasian, 15-month-old donor, Lonza, #HUM181791; ThermoFisher, #CY409, respectively).
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bioRxiv - Biochemistry 2024Quote: ... The preparations of insect cell microsomes containing human CYP2A6 (lot 2520331) and CYP2D6 (lot 2350106) along with human CPR and cytochrome b5 (Baculosomes®) were procured from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Genomics 2024Quote: Dataset 3: This dataset includes the Human Origins SNP Array genotypes (2,583 present-day individuals genotyped on the Affymetrix Human Origins Array) merged with ancient individuals from SW Asia and S Europe called across a total of 616,938 autosomal SNPs (Lazaridis et al. ...
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bioRxiv - Cell Biology 2019Quote: ... protein was extracted using 0.2M Sodium Hydroxide and protein concentrations were determined using the Pierce™ 660 protein assay (Fisher Scientific), according to manufactures instructions ...
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bioRxiv - Pathology 2020Quote: ... Fractions were tested for protein concentration using 5 μL of protein sample mixed with 10 μL Coommassie Protein Assay reagent (Thermo Scientific). Pooled fractions were concentrated to 4 mL using 10 kD molecular weight cut-off spin columns (Millipore ...
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“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: Collected protein flow-throughs (FT) were subjected to protein precipitation for downstream protein content determination using bicinchoninic acid assay (BCA, Thermo Fisher) assay and trypsin protein digestion ...
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bioRxiv - Cell Biology 2021Quote: ... The protein concentrations of the lysates were measured by BCA protein assay using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and then unified to 0.5 µg/µL ...
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bioRxiv - Biochemistry 2022Quote: Protein concentration after cell lysis and after protein precipitation was determined using the Micro BCA™ Protein assay kit (Thermo Scientific) according to manufacturer’s guidelines.
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bioRxiv - Neuroscience 2019Quote: ... Newly synthesized proteins were then enriched using the Click-it protein enrichment kit for chemistry capture of azide modified proteins (Invitrogen C10416) following the manufactures protocol with small modifications ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein expression was performed according to Culturex protocol while protein purification was done using Pierce Protein A Agarose kit (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Measurement of final protein concentration was performed using BCA protein assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific™, #23227), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Soluble marker protein levels were normalized to total protein levels measured by Quant-iT protein Assay Kit (Thermo Fisher Scientific, USA).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were supplemented with SDS to a final concentration of 2% and the protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Neuroscience 2022Quote: Recombinant NRX1β-Fc and NRX1βΔHRD-Fc proteins were pre-immobilized with Protein G Magnetic Beads (Dynabeads Protein G, Cat# 10004D, Thermo Fisher Scientific) in PBS overnight at 4 °C with constant agitation ...
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bioRxiv - Immunology 2024Quote: ... Protein concentrations across all samples were normalised to the same protein concentration (500 µg/ml) with protein determination quantified using the well-established Pierce BCA Protein Assay (Thermo Fisher) following the recommended guidelines provided by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... and supernatant removed for protein concentration analysis using a BCA protein assay (Pierce BCA Protein Assay Kit; ThermoFisher Scientific; cat # 23227). Twenty μg samples were loaded into SDS-PAGE gels and transferred onto Amersham Hybond-P 0.45μm PVDF membranes (GE Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were cleared by centrifugation and protein concentration was quantified using the Qubit™ Protein and Protein Broad Range Assay Kit (ThermoFisher). Protein samples were sent to the UNSW Bioanalytical Mass Spectrometry Facility for liquid chromatography-mass spectrometry (LC-MS/MS ...
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bioRxiv - Neuroscience 2023Quote: ... Fractions were tested for protein concentration using 5 μL of protein sample mixed with 10 μL Coomassie Protein Assay reagent (ThermoFisher Scientific). Pooled fractions were concentrated to 4 mL using 10 kDa molecular weight cut-off spin columns (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentration in supernatant was determined using bicinchoninic acid (BCA) protein assay (PierceTM BCA Protein Assay Kit, Catalog #23225, ThermoFisher Scientific). 30-40 µg total protein sample was denatured at 75 °C for 7.5 min and then electrophoresed using polyacrylamide gel (4–15% tris-glycine extended polyacrylamide gel ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted proteins/complex were subject to SDS-PAGE electrophoresis followed by protein staining (SYPRO Ruby Protein Gel Stain, Thermo Fisher Scientific) and immunoblotting.
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bioRxiv - Physiology 2024Quote: ... The protein content of the EV preparations was quantified by bicinchoninic acid protein assay (Pierce™ BCA Protein Assay Kit, Thermofisher).
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bioRxiv - Developmental Biology 2024Quote: ... The purified proteins were stored at −80°C and the protein concentration was determined using Bradford plus protein assay reagents (23238, Thermo Scientific).
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bioRxiv - Neuroscience 2024Quote: ... and protein concentration in the lysates was measured using a bicinchoninic acid protein assay kit (Micro BCA Protein Assay Kit, Thermo Scientific). Equal amounts (750 micrograms to 1 mg depending on the experiment ...
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bioRxiv - Developmental Biology 2021Quote: Human iPSCs were cultured in StemPro hESC SFM (Gibco) supplemented with 20ng/ml bFGF (R&D ...
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bioRxiv - Genomics 2021Quote: ... and HLA-DR/human MHC class II antibodies (Invitrogen Dynabeads® ...
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bioRxiv - Microbiology 2021Quote: ... Human hepatocellular carcinoma cells (Huh-7; Thermo Fisher Scientific) and African green monkey kidney cells (Vero E6 ...
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bioRxiv - Immunology 2022Quote: ... and CD3/CD28 Human T-activator Dynabeads (Thermo Fisher) at a 1:1 bead to cell ratio ...
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bioRxiv - Molecular Biology 2020Quote: ... Commercial pooled RNA (Quantigene Human Reference, QS0639, Life Technologies) and pooled human male DNA (G3041 ...
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bioRxiv - Molecular Biology 2021Quote: ... MEFs and human fibroblasts were cultured in DMEM (Invitrogen) supplemented with 10% FBS (Atlanta Biologicals ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... and Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher) at 1 bead per cell ...
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bioRxiv - Genomics 2020Quote: ... or Mouse anti-human GAPDH monoclonal (MA5-15738, Invitrogen). The secondary antibody was HRP conjugated Goat anti-Rabbit (NA934 ...
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bioRxiv - Cell Biology 2019Quote: Unlabeled LDL (1 mg) from human plasma (Thermo Scientific) was incubated with 50 nmol cholesterol (Top-Fluor cholesterol ...