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Citations for Thermo Fisher :
2351 - 2400 of 8195 citations for Recombinant Human MCAM Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Mouse bone marrow cells were isolated by crushing entire long bones into DMEM/3% FCS (GIBCO). Bone fragments were removed by filtration through a 70 μm filter mesh ...
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bioRxiv - Microbiology 2020Quote: COS7 and HEK293T cells were maintained in DMEM supplemented with 10 % FCS and GlutaMAX (Life Technologies), 5 % CO2 ...
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bioRxiv - Immunology 2021Quote: ... washed in TBS and blocked with StartingBlock T20 Blocking buffer containing Fc blocker (Thermo Scientific, 37543). Sections were incubated with unconjugated L ...
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bioRxiv - Immunology 2021Quote: ... mice were directly perfused with PBS/ 2 % FCS/ 1 mM EDTA and liver digest medium (ThermoFisher Scientific/ Gibco ...
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bioRxiv - Immunology 2021Quote: ... The optical density was measured at 450 nm with a Multiscan FC Plate Reader (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... was measured as absorbance at 450 nm (OD450) by Spectrophotometer (Thermo Scientific, Multiskan FC Microplate Photometer). The titers were defined from the reciprocal value of the absorbance at OD450.
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bioRxiv - Immunology 2020Quote: ... ACE2-Fc was purified to homogeneity from the conditioned medium using PoRos XQ column (Thermo Fisher) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: Cells were then subjected to Fc blockade with anti-CD16/CD32 (Thermo Fisher Scientific, Waltham, MA), washed with 1XPBS ...
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bioRxiv - Immunology 2022Quote: ... The isolated lungs were placed in RPMI-1640 containing 2% (vol/vol) FCS (Thermo Fisher Scientific), 50 μg/ml Liberace TM (Roche ...
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bioRxiv - Immunology 2022Quote: ... explanted organs were stored in 600 μL DMEM containing 10% FCS and 1% Penicillin/Streptomycin (Gibco). After mechanical disruption by shaking at 25/s over 4 min with metal beads (TissueLyser II ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% (vol/vol) fetal calf serum (FCS, Biochrom) and antibiotics (1x Pen/Strep, Gibco). tRF were generated from primary rhesus fibroblasts (RF ...
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bioRxiv - Immunology 2023Quote: ... The IgGs were purified by adding Protein G (Cytiva) or CaptureSelect IgG-Fc resin (Thermo Scientific) to the supernatant and rolling overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were cultivated in DMEM-F12 medium containing 20% fetal bovine serum (FCS, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... hDPP4-Fc and G2 were biotinylated using EZ-Link™ Sulfo-NHS-LC-Biotin (Thermo Scientific) and 80% maximum binding concentrations against S-2P were determined with biotinylated proteins (hDPP4-Fc-biot and G2-biot) ...
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bioRxiv - Microbiology 2024Quote: ... Proteins fused with Fc were captured using Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424), eluted with pH 3.0 glycine (100 mM in H2O) ...
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bioRxiv - Systems Biology 2023Quote: MetR medium was prepared by titrating 0% MetR medium (complete DMEM, ThermoFisher, 21013-024, 10% FCS) with 100% MetR medium (complete DMEM without L-methionine and L-cysteine ...
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bioRxiv - Systems Biology 2023Quote: ... cells were washed and maintained in regular culture medium (DMEM GlutaMAX™, Gibco®, 10% FCS) for 5 days ...
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bioRxiv - Immunology 2023Quote: ... blocked with Fc-block and stained with LiveDead APCe780 (1:1000; Thermo Fisher, #65-0865-14), APC anti-CD19 (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... prior to resuspension in complete RPMI (with 10% FCS, 1% Penicillin/Streptomycin, 1% L-glutamine, Gibco) and passing through a 70 μm cell strainer ...
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bioRxiv - Immunology 2023Quote: ... After treating the cells for 15 minutes (min) with Fc Receptor Binding Inhibitor Polyclonal Antibody (ThermoFisher), they were stained for 30 min at 4°C in the dark and washed twice again ...
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bioRxiv - Immunology 2023Quote: ... Optic density was then measured at 405 nm on a Multiskan FC microplate photometer (Thermo Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... and -10 Fcs were assessed by detecting with an anti-mouse IgG-specific secondary (Thermo Fisher) and analyzed via flow cytometry (BD Biosciences).
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bioRxiv - Bioengineering 2023Quote: Ly6a-Fc was produced in Expi293F suspension cells grown in Expi293 Expression Medium (Thermo Fisher Scientific) in a 37 °C ...
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bioRxiv - Bioengineering 2023Quote: Ly6a-Fc was produced in Expi293F suspension cells grown in Expi293 Expression Medium (Thermo Fisher Scientific) in a 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... medium of the HEK293T cells was changed to transfection medium containing DMEM and 10% FCS (Gibco), incubating the cells for 30 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... The tissue was incubated 2 times in RPMI 1640/5%FCS supplemented with 5mM EDTA (Gibco) for 20min each at 37°C and 200rpm ...
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bioRxiv - Bioengineering 2023Quote: ... The sections were then incubated with WA2.22 Fc fusion (100 nM) and AT8 (1:200, Invitrogen) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... KELLY cells were cultured in RPMI medium with 10-15% fetal calf serum (FCS) (Life Technologies). Cells were maintained at 5% CO2 and at 37°C and were split at full confluency (at 5% CO2 and at 37°C ...
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bioRxiv - Immunology 2023Quote: ... cells were first incubated with an anti-CD16/CD32 Monoclonal Antibody to block FC receptors (Invitrogen). Cells were washed using PBS and incubated with LIVE/DEAD™ dye for 30 minutes at 4°C ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... ALK1-Fc-complexes were then captured with CaptureSelect FcXL Affinity Matrix (Cat: 194328010, Thermo Fisher Scientific). The ALK1 ligand-depleted 30% BSA was diluted to 0.2% with phenol red-free EBM2 for use.
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bioRxiv - Immunology 2023Quote: ... Raji cells and CHO cells were cultured in complete RPMI-1640 (Gibco, 10% FCS, 2mM GlutaMAX), and HEK293T cells ...
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bioRxiv - Immunology 2023Quote: ... prior to resuspension in complete RPMI (with 10% FCS, 1% Penicillin/Streptomycin, 1% L-glutamine, Gibco) and passing through a 70 μm cell strainer ...
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bioRxiv - Microbiology 2023Quote: Isolated monocytes were resuspended in complete RPMI media with 2mM L-glutamine and 5% FCS (Gibco) and seeded in a 24-well cell culture plate at 5 x 105 cells/well ...
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bioRxiv - Developmental Biology 2023Quote: ... Optical density values were determined using a Multiskan™ FC Microplate Photometer (Thermo Scientific™ 51119000).
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS) (Gibco A5670401; Thermo Fisher Scientific, Waltham, MA) and 1% (v/v ...
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bioRxiv - Synthetic Biology 2024Quote: ... after which adsorption was measurement at 450 nm using a microplate reader (Multiscan FC, Thermo Scientific).
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10 % (v/v) FCS and 0.5 % (v/v) Insulin-Transferrin-Selenium (Thermo Fisher Scientific). For the experiment ...
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bioRxiv - Immunology 2024Quote: ... prior to resuspension in complete RPMI (with 10% FCS, 1% penicillin/streptomycin, 1% L-glutamine; Gibco) and passing through a 70 µm cell strainer ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% fetal calf serum (FCS) (#S181BH-500, Biowest) and 1% penicillin-streptomycin (#15140122; Gibco). Purity > 97% of CD14+ monocytes after magnetic sorting was verified by flow cytometry (anti-CD14-FITC clone MφP9 ...
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bioRxiv - Biophysics 2021Quote: ... mCherry-tagged protein-expressing Neuro2a cells were incubated in a medium containing 0.5 μM Lysotrascker green DND-26 (Thermo Fisher) for 30 min before image acquisition.
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bioRxiv - Developmental Biology 2021Quote: Immunoprecipitations from HEK293T cells expressing either HA-or FLAG-tagged Yap were performed using the Dynabeads Protein A Immunoprecipitation Kit (Invitrogen) by coupling 5 μg of anti-FLAG (Cell signaling ...
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bioRxiv - Cell Biology 2020Quote: ... The Flp-In T-Rex HEK293 cells were transfected with the N-terminal GFP-tagged FAM83F packaged in a pcDNA5-FRT/TO vector along with Flp recombinase pOG44 (Invitrogen) in a ratio of 1 μg ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was then incubated with appropriate antibodies for 12 h and then conjugated with dynabeads Protein A or Protein G beads for an additional 1 h SFB- or GFP-tagged proteins were immunoprecipitated with streptavidin dynabeads (Invitrogen) or GFP-Trap (Chromotek) ...
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bioRxiv - Developmental Biology 2021Quote: ... The following primary antibodies were used: mouse anti-V5-tag to amplify V5-tagged CFP (Thermo Fisher, R96025, 1:650), rat anti-mCherry (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: 6xHis-tagged REV-ERBβ LBD (4 nM) was incubated with anti-His antibody conjugated to terbium (1 nM) (ThermoFisher #PV5863) and FITC-labeled NCoR ID1 or NCoR ID2 peptide (400 nM ...
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bioRxiv - Neuroscience 2019Quote: ... Chicken anti-rabbit tagged with Alexa Fluor 488 and goat anti-mouse tagged with Alexa Fluor 568 were used as secondary antibody at a dilution of 1:1000 (Invitrogen). Tissues were incubated with these secondary antibodies for 48 hours at 4 °C followed by regular washing ...
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bioRxiv - Molecular Biology 2020Quote: ... MBP-tagged full-length TRIM21 was cloned into a pFastBac vector for expression in the baculovirus system (Bac-to-Bac, Invitrogen), resulting in pFastBac-MBP-TRIM21 ...
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bioRxiv - Molecular Biology 2020Quote: ... Large-scale expression of Strep-tagged proteins for purification was conducted in Trichoplusia Ni High Five cells (Thermo Fischer Scientific) in SF-900 medium (Gibco). Cells at a density of 2×106 cells/ml were infected with 1.5% of amplified virus and incubated for 40 hours ...
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bioRxiv - Bioengineering 2022Quote: ... supernatants were harvested and filtered with a 0.22 µm membrane.The His-tagged proteins were purified with the HisPur Ni- NTA Resin (Thermo Fisher, 88222). After three columns of washing with 25 mM Imidazole (pH 7.4) ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse IP6K1 was sub-cloned into SFB (S-protein/FLAG/SBP) - tagged destination vector using the Gateway cloning strategy (Invitrogen). Catalytically inactive IP6K1 (K226A ...