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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for Mouse Anti HIV 1 p24 Recombinant Antibody clone 183 H12 5C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... primary antibodies were incubated for three nights at 4°C followed by incubation with donkey anti-mouse or anti-rabbit IgG secondary antibodies (1:1000, Life Technologies). Fluorescent images were acquired with an Olympus FV1000 at 2048 x 2048 pixel resolution using a 40x objective lens ...
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bioRxiv - Pathology 2021Quote: ... followed by secondary antibody incubation (1hr, RT, goat anti-rabbit alexa 594 and goat anti-mouse alexa 488, 1:500, Fisher Scientific). Stained sections were treated with Sudan Black (0.03% Sudan Black B in 70% ethanol ...
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bioRxiv - Immunology 2020Quote: ... the bound antibodies were detected by incubation with horseradish peroxidase (HRP)-conjugated anti-mouse IgG or anti-Macaca fascicularis IgG antibody (1:5,000, Thermo Fisher Scientific) for 30 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... samples were washed with PBTX for three times and replaced with secondary antibody (1%, goat anti-mouse Alexa Fluor 488 and goat anti-rat Alexa Fluor 568, ThermoFisher UK) and 1 ug/ml DAPI solution ...
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bioRxiv - Bioengineering 2023Quote: ... sections were counterstained for 1 hour at room temperature with secondary antibodies (donkey anti-rabbit Alexa Fluor 555 and donkey anti-mouse Alexa Fluor 647; 1:200; Thermo Fisher) and DAPI (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Horseradish peroxidase– conjugated secondary antibodies, goat anti-mouse immunoglobulin G (IgG) (# 32230), and goat anti-rabbit IgG (# 32260, 1:5000) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-mouse and anti-rabbit Alexa fluor 488/568 conjugated secondary antibodies were incubated at 1:800 dilutions (Thermo Fisher Scientific). The cells were mounted in Vectashield (Vector Laboratory ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated overnight at 4°C with secondary antibodies conjugated with Alexa Fluor 633 (1:500; Goat anti-Rabbit Invitrogen A21070, Goat anti-Mouse Invitrogen A21053).
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bioRxiv - Microbiology 2023Quote: ... coverslips were washed three times in PEMBALG and incubated with Alex Fluor 488 goat anti-rabbit and/or Alex Fluor 594 goat anti-mouse antibodies (1:1000; Life Technologies) for four hours at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies were AlexaFluor 405 goat anti-rabbit for PV or AlexaFluor 594 goat anti-mouse (1:500, ThermoFisher Cat# A31556).
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bioRxiv - Cell Biology 2024Quote: ... coverslips were washed three times for 10 min and then incubated with the appropriate secondary antibodies (1:500, Alexa donkey anti-rabbit 568, Invitrogen, A10042, or Alexa donkey anti-mouse 488, Invitrogen, A21202) for two hrs at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary goat anti-rabbit (#31460) or goat anti-mouse (#31430) antibodies conjugated to horseradish peroxidase (HRP) (Thermo Scientific; 1:5,000 dilution) were used for detection ...
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bioRxiv - Cell Biology 2024Quote: ... before 3x PBS Ca2+ washes and incubated for 1 hour at RT with anti-mouse 488 ± anti-rabbit 555 secondary antibodies (ThermoFisher Scientific). Cells were imaged directly in IBIDI chambers using an Oxford Nanoimager (Oxford Nanoimaging ...
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bioRxiv - Immunology 2024Quote: ... followed by 2 h incubation with HRP-conjugated secondary antibody (1:10000; anti-mouse or anti-rabbit, Invitrogen #31430 and #31460) in TBST ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibodies were goat anti-mouse Alexa Fluor 488 or goat anti-rabbit Alexa Fluor 594 (Molecular Probes; 1:200). The incubation with antibodies was carried out in a humidified chamber for 1 h at RT.
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bioRxiv - Genetics 2023Quote: ... cells were incubated for 1 hour at room temperature with secondary antibodies (Alexa555 - donkey anti-rabbit and Alexa 488 - donkey anti-mouse, 1:2000, Thermofisher Scientific) and DAPI diluted in the same buffer ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with secondary antibody (AlexaFluor 546 donkey anti-mouse for PV, goat anti-rabbit for GluR2/3 or NPY, both 1:400, Thermo Fisher). Secondary antibody was dissolved in 1% normal donkey serum for PV or 1% normal goat serum for GluR2/3 or NPY ...
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bioRxiv - Immunology 2022Quote: ... The secondary antibody mixture contained anti-rabbit AlexaFluor-555 and anti-mouse AlexaFluor-488 (all purchased from ThermoFisher Scientific; 1:250). Nuclear counterstaining was performed using SlowFade Gold antifade reagent with DAPI (Invitrogen) ...
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Single-cell assessment of trophoblast stem cell-based organoids as human placenta-modeling platformsbioRxiv - Developmental Biology 2022Quote: ... sections and coverslips were washed with PBS and incubated with Alexa Fluor goat anti-rabbit 568 and goat anti-mouse 488 conjugated secondary antibodies (1:200; A11036, A32723; Life Technologies) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then incubated at RT for 2 hours with 1:1000 Alexa Fluor (AF) 647 donkey anti-mouse and 1:200 of AF488 donkey anti-rabbit fluorescence conjugated secondary antibodies (Life Technologies). The cover slip was mounted in mounting solution with DAPI ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were washed 3 times in TBS-T for 10 minutes each and incubated with an alkaline phosphatase-linked secondary goat anti-mouse/anti-rabbit antibody (1:10,000; Thermo Scientific Pierce) at RT for 1h ...
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bioRxiv - Developmental Biology 2023Quote: ... slides were incubated 45-60 min at room temperature with secondary antibodies in PBT (1:500 goat anti-rabbit-Alexa488 and goat anti-mouse-Alexa568 (ThermoFisher Scientific). Slides were counterstained with 1 μg/ml Hoechst 33258 (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... with AF488-conjiugated secondary antibodies diluted 1:3000 in BPS (goat anti-mouse IgG, A11001 or goat anti-rabbit IgG, A11008, Life Technologies). For dual-color IF ...
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bioRxiv - Neuroscience 2024Quote: ... Next day sections were washed 3×5 minutes with PBS and incubated with secondary antibodies for 2 hours at room temperature diluted in antibody buffer (Goat-anti-Rabbit Alexa 488 ThermoFisher A11034, 1:500, Goat-anti-Mouse Alexa 647, ThermoFisher 21058). Then sections were washed again for 3×5 minutes in PBS ...
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bioRxiv - Physiology 2024Quote: ... and secondary antibodies AF488-coupled goat-anti-rabbit IgG and AF594-coupled goat-anti-mouse IgG (1:1,000, Thermo Fisher Scientific) in presence of 0.2% saponin ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then incubated for 60 minutes at room temperature with Alexa-Fluor 546 goat anti-mouse or anti-rabbit secondary antibody (1:200; Thermo Fisher). Images were acquired using a widefield microscope (Leica DM6000 ...
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bioRxiv - Cell Biology 2023Quote: ... and then incubated with the indicated primary antibodies (mouse anti- MIC60 [110329; Abcam], rabbit anti-MIC27 [PA5-51427; Thermo Fisher Scientific], mouse anti- TIMM23 [BDB611222; Thermo Fisher Scientific] ...
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bioRxiv - Biophysics 2022Quote: ... coverslips were incubated for 2 hours in a 1:1000 dilution of secondary antibody (anti-rabbit Alexa 647, Invitrogen, A-27040; anti-mouse Alexa 647, Invitrogen, A-32728, anti-mouse Alexa 488, Invitrogen, A-32723). Coverslips were washed 3 times with blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... Standard Western blotting procedures were used with the following antibodies: HIV-1 gp120 (NIH AIDS Reagent program catalog no. 288) and 6x-His Tag (ThermoFisher catalog no. MA1-21315-HRP).
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bioRxiv - Neuroscience 2020Quote: ... to enhance viral expression (primary antibody: GFP Recombinant Rabbit Monoclonal Antibody, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... anti-Zonula occludens-1 (ZO-1) antibody (Invitrogen), goat anti-rabbit secondary antibody (Life technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... or anti-Phospho-FOXO3a-antibody (1:200; Invitrogen Antibodies), respectively ...
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bioRxiv - Microbiology 2021Quote: ... ACE2 expression was assessed by staining cells with polyclonal rabbit antibodies against human ACE2 (PAB886Hu01, Cloud-Clone Corp.) followed by secondary anti-rabbit antibodies conjugated with PE (1:250, # P-2771MP, ThermoFisher Scientific). Samples were analyzed on CytoFLEX S flow cytometer (Beckman Coulter) ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-actin primary antibody and with Alexa Fluor 647 anti-Rabbit and Alexa Fluor 488 anti-Mouse secondary antibodies (Molecular Probes). DNA was counterstained with DAPI ...
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bioRxiv - Bioengineering 2022Quote: ... Mouse anti-ZO-1 (#33-9100; Life Technologies; 1:125), Rabbit anti-ECAD (#3195S ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-V5 tag (Invitrogen, R960-25, 1:1:500), and mouse anti-αTUBULIN (B512 ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-ZO-1 (Invitrogen cat. # 33-9100; 1:100), mouse anti-ARL13b (UC Davis / NIH ...
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bioRxiv - Molecular Biology 2023Quote: ... mouse anti-TY-1 1:2000 (Thermo Fisher, MA5-23513), or mouse anti-actin 1:10,000 (Abcam ab3280) ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-PECAM-1 (Thermo Fisher, MA5-13188, 1:1000). The secondary antibodies we used in this study are listed below ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-ZO-1 (mouse IgG1, 1/500, 33-910, ThermoFisher), anti-GFP (chicken ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His Tag Antibody (clone HIS.H8) (1:1000, # MA1-21315) was from ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... In some experiments, αIFNγ neutralizing antibody (clone XMG.1, 2 μg/mL) (Thermofisher) was added to a cell culture ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was collected and incubated with mouse anti-GFP clone 3E6 (Molecular Probes, Figure 6E) for 1 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The mouse anti-CHIKV (Clone A54Q, Cat# MA5-18181) and Lipofectamine 2000 was obtained from ThermoFisher Scientific (Rockford ...
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bioRxiv - Neuroscience 2023Quote: ... AF 488 rat anti-mouse TMEM119 (cat # 53-6119-82, Invitrogen, clone V3RT1GOsz, 5 ng/μl), and Alexa 488 rat anti-mouse NeuN (cat # MAB377X ...
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bioRxiv - Immunology 2023Quote: ... Epidermal sheets were stained with FITC-anti-mouse TCRγδ (Clone GL3) and TOPRO-3 Iodide (ThermoFisher). For determination of DETC contribution to skin S ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-Caspase-8 (clone B.925.8; RRID:AB_10978471; 0.619g/L Thermo Fisher Scientific Cat# MA5-15226), mouse anti-Caspase-8 (clone 5D3 ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody solution contained 2 secondary antibodies in blocking solution: Goat anti-mouse Alexa-Flour (AF) 568 (Invitrogen A-11004, 1:400), Goat anti-chicken AF-488 (Invitrogen A11039 ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% PFA for 30 minutes and stained for intracellular ENV with 1A1D-2 (1/500) monoclonal antibody and anti-mouse 647-conjugated antibody (2 μg/ml, Invitrogen Cat# A21235).
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Purkinje cardiomyocytes of the ventricular conduction system are highly diploid but not regenerativebioRxiv - Developmental Biology 2022Quote: ... heart tissue sections were incubated at room temperature for 2 hr with secondary antibodies: goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody AlexaFluor 488 conjugate 1:500 (A11001, Thermo Fisher Scientific); goat anti-rabbit IgG (H+L ...