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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for Human Anti NT5E Recombinant Antibody clone Hu101 28 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Purified recombinant HisHA-INF1 or HisHA-Scr74 were incubated with 20 μl Pierce Anti-HA magnetic beads (Thermo Fisher Scientific) at an amount of 500 pmol each for 30 min ...
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bioRxiv - Immunology 2020Quote: ... A total of 100.000 T cells were co-cultured with 50,000 sorted myeloid cells in the presence of 30IU recombinant hIL-2 plus anti-CD3/CD28 Dynabeads (ThermoFisher Scientific) for 3-4 days in round-bottom plates ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1:1 with nonMDSCs in the presence of 100 IU recombinant IL-2 plus mouse anti-CD3/CD28 Dynabeads (cat # 11453D, Gibco) for 4 days in a 96-well round-bottom plate ...
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bioRxiv - Cancer Biology 2024Quote: ... human FGF and human EGF supplement (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... by recovering fragments of 28 to 30 nt after gel staining with SYBR Gold (Thermo Fisher Scientific, USA), using Truseq Ribo Profile RNA Control as reference ...
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bioRxiv - Bioengineering 2020Quote: ... All sequences were cloned into the entry vector pDRf1-GW for yeast expression (28) through Gateway cloning (Invitrogen).
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bioRxiv - Bioengineering 2022Quote: ... [28] Upon reaching ≥ 90 % confluence (day 0) media was exchanged to RPMI 1640 medium (RPMI; 1185063, Gibco, USA) supplemented with B27 supplement without insulin (B27-I ...
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bioRxiv - Cell Biology 2022Quote: ... mexicana promastigotes (WHO strain MNYC/BZ/62/M379) were cultured at 28°C in M199 medium (Life Technologies) supplemented with 2.2 g/L NaHCO3 ...
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bioRxiv - Microbiology 2020Quote: ... divergens cultures of at 25-28% parasitaemia were stained with MitoTracker Green FM mitochondrial stain (Thermo Fisher Scientific) at a final concentration of 500 nM (6) ...
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bioRxiv - Pathology 2020Quote: ... the cells of K562 and K562−28(A>G) were cultured in glutamine-minus RPMI 1640 medium(Gibco) with10% FBS in the presence of 1 mM sodium butyrate for 7 days to induce the erythroid differentiation ...
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bioRxiv - Cell Biology 2020Quote: ... ZMEL cells were grown at 28°C in a humidified incubator in DMEM (Gibco, Waltham, USA, Catalog, #11965) supplemented with 10% FBS (Gemini Bio ...
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bioRxiv - Plant Biology 2022Quote: ... pTAN1:CFP-TAN1 and pTAN1:CFP-TAN1(28-33A) transformants were selected on 100μg/mL gentamicin (Fisher Scientific) and the presence of mScarlet-MAP4 ...
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bioRxiv - Biochemistry 2022Quote: ... (Syx*1) and (Syb*28) were labelled with Oregon Green 488 Maleimide according to the manufacturer’s instructions (Invitrogen). Fluorescence anisotropy was recorded at λemi=524 nm upon excitation at λexc=496nm ...
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bioRxiv - Cell Biology 2024Quote: ... we added 25uL of washed and TCM + 40U/mL IL-2 resuspended CD3/28 Dynabeads (Gibco, Cat#: 11131D) to each well ...
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bioRxiv - Microbiology 2020Quote: ... goat anti-human IgG labeled with FITC (Chemicon) or goat anti-rabbit IgG labeled with Alexa 488 (Thermo Scientific) was used diluted at 4 μg/mL in PBS and incubated for 30 min with 5 μg/mL 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI ...
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bioRxiv - Immunology 2021Quote: ... Anti-human IgM and IgG conjugates provided within the kit were replaced with anti-mouse IgM-HRP-labeled (Invitrogen) and anti-mouse IgG-HRP-labeled (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... Naive and memory T cells were then stimulated with anti-CD3/anti-CD28 human T-Activator Dynabeads® (Invitrogen) at a 1:2 beads-to-cells ratio ...
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bioRxiv - Immunology 2024Quote: ... primary human CD4 T cells were activated with anti-CD3+ anti-CD28 Dynabeads (1:1) (Thermofisher scientific, cat # 11161D) + IL-2 overnight and then 1 million aliquots of cells (triplicates ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit polyclonal anti-β-PIX antibody (4515S; Cell Signalling Technology) and rabbit polyclonal anti-GST antibody (71-7500; Invitrogen).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... immunohistochemistry for UCP1 (primary anti–UCP1 antibody: Abcam, Catalog# ab209483; secondary antibody: goat anti–rabbit IgG, Invitrogen, Catalog# 32260), or fluorescence for AF488 (conjugated to MS-Hu6 ...
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bioRxiv - Microbiology 2021Quote: ... Typhi lipopolysaccharide (Sifin) antibodies followed by Alexa 488-conjugated anti-mouse and Alexa 594-conjugated anti-rabbit antibodies (Invitrogen). Samples were visualized under a Nikon TE2000 fluorescence microscope equipped with and Andor Zyla 5.5 CMOS camera driven by Micromanager software (https://www.micro-manager.org) ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was further probed with 1:10,000 rabbit anti-PfExp2 primary antibody followed by 1:10,000 HRP conjugated secondary anti-rabbit antibody (Thermo Fisher) for 1h ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with Alexa FluorTM 594 anti-rabbit antibody and Alexa Fluor® 488 anti-mouse antibody (Invitrogen) of 2 drops/ml for 30min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibodies used were: anti-mouse and anti-rabbit HRP-conjugated secondary antibodies (Thermo Fisher; 61-6520 and 31460); anti-mouse IRDye 800CW secondary antibodies (LICOR) ...
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bioRxiv - Microbiology 2021Quote: Secondary anti-IgG antibodies and their sources were: Alexa Fluor Plus 594 goat anti-rabbit antibody (Invitrogen, Rockford, USA). Horseradish peroxidase linked donkey anti-rabbit (GE Healthcare ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by IHC with anti-GFP primary antibody (abcam290) and goat anti-rabbit Alexa 488 secondary antibody (Invitrogen A11011).
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bioRxiv - Microbiology 2021Quote: ... APC-conjugated goat anti-mouse and ducky anti-rabbit antibodies were used as secondary antibodies (1:100 dilution, Invitrogen), and expression was detected by flow cytometry (Guava easyCyte) ...
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bioRxiv - Microbiology 2022Quote: ... Primary antibodies were detected with anti-mouse Alexa-Fluor 488 and anti-rabbit Alexa-Fluor 594 secondary antibodies (Invitrogen). Images were acquired with an Eclipse Ti microscope (Nikon ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Secondary antibodies were anti-mouse Alexa Fluor 594 and anti-rabbit Alexa Fluor 488 antibodies (Thermo Fisher, MA, USA).
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bioRxiv - Microbiology 2024Quote: ... Anti-GFP polyclonal antibody and secondary goat-anti-rabbit HRP conjugate antibody were supplied by Thermo Scientific (Ulm, Germany) and used at the dilutions recommended by the manufacturer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Alexa 488-conjugated goat anti-rabbit antibody and Alexa 594-conjugated goat anti-mouse antibody were obtained from Invitrogen Life Technology (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated with chicken anti-GFP antibody (1:250) and/or mouse monoclonal anti-AnkB antibody 33-3700 (Thermofisher) (1:250 ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibodies used were: goat anti-mouse and goat anti-rabbit horseradish peroxidase (HRP)-conjugated antibodies (Thermo Fisher Scientific), donkey anti-goat and anti-rabbit IgG Alexa Fluor 594/ 488 conjugates (Invitrogen).
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: ... RNA-protein complexes were immunoprecipitated using 5 μg antibody (either anti-ZBTB48, or anti-GFP antibody (Life Technologies G10362), or anti-FLAG ...
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bioRxiv - Cell Biology 2020Quote: ... Cell pellets were incubated with a 1:200 dilution of human cTnT primary mouse antibody (ThermoFisher) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Antibody production was detected using the ELISA human IgG total kit (Thermo Fisher, #88-50550-22) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... washed twice with PBS and stained with anti-SARS-CoV-2 (1A9; Biozol GTX-GTX632604) anti-MUC5AC (clone 45M1; MA1-21907, Thermo Scientific) and anti-alpha-tubulin (MA1-8007 ...
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Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: ... single cells were re-suspended at a density of 1-2 × 107 and subjected to magnetic sorting to exclude immune cells using rat-anti-mouse CD45 (R&D, MAB114) and CD24 (eBioscience, clone M1/69) magnetic sheep-anti-rat Ig Dynabeads (Invitrogen, #11035). FACS for FITC+/RFP-/DAPI-was used to obtain GFP+ve CAFs and to exclude RFP+ve tumour cells and dead cells ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-claudin-4 mAb (#32-9400), rabbit anti-tricellulin mAb (clone 54H19L38, #700191) and rabbit anti-ZO-2 pAb (#71-1400) were purchased from Thermo Fisher Scientific (MA ...
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bioRxiv - Immunology 2024Quote: ... The membranes were blocked using a 8% skim milk for 2 hours and then incubated with mouse anti-bass IgM mAb (clone 66, IgG1, 1 μg/mL) and then incubation with HRP goat-anti-mouse IgG (Invitrogen, USA) for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... human (Invitrogen). Cells were cultured in DMEM (Corning Cellgro 10-013-CV ...
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bioRxiv - Microbiology 2022Quote: ... mCherry was detected using rabbit anti-mCherry polyclonal antibody (Life Technology PA534974) as a primary antibody in combination with an anti-rabbit secondary antibody (Thermo Fisher Scientific, catalogue no. 31460).
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bioRxiv - Neuroscience 2023Quote: Sections were washed in PBS three times for 5 min each and incubated in secondary antibodies in PBS (goat or donkey anti-rabbit, anti-mouse, or anti-rat fluorescent antibodies; Invitrogen Alexa Fluor; 1:500) for 1 h at room temperature in the dark ...
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bioRxiv - Synthetic Biology 2021Quote: ... CD3+ T cells were activated with anti-human CD3/CD28 magnetic Dynabeads (CTS, ThermoFisher) in a 1:1 ratio with 100 U mL-1 of IL-7 and 10U mL-1 IL-15 (R&D Systems ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were incubated with Alexa Fluor 488 goat anti-human IgG(H+L) (Invitrogen) at 1:500 (in 3% BSA/PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the cells were stained with AlexFuor488-conjugated goat anti human Fc (Life technologies) at RT for 30 min and analyzed by flow cytometry ...
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bioRxiv - Cell Biology 2021Quote: ... FITC conjugated F(ab’)2 goat anti-human IgA (Thermo Fisher Scientific, Illkirch, France), Brilliant Violet 650 conjugated streptavidin (BioLegend ...
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bioRxiv - Microbiology 2022Quote: ... The goat anti-human IgG conjugated with Alexafluor-647 was purchased from Invitrogen (A21445).
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bioRxiv - Immunology 2020Quote: ... T-cells were incubated with murine or human anti-CD3/CD28 Dyna Beads (ThermoFisher) for 48 hours unless noted otherwise ...
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bioRxiv - Immunology 2021Quote: ... and anti-human AlexaFluor 647 (1:500, 30 minutes, 4°C; Life Technologies, A21445), and resuspended in CellFix (1:10 in water ...