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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with 2 µM Hoechst33342 (Molecular probes, Thermo Fisher Scientific) for 10 min and mounted in Mowiol with 1.4 % w/v DABCO ...
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bioRxiv - Cancer Biology 2023Quote: ... B16 cells were also supplemented with freshly added 2 mM L-glutamine (Invitrogen). Mice were divided into groups randomly while satisfying the criteria that the average body weight in each group is similar ...
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bioRxiv - Cell Biology 2023Quote: ... The cells sediment was added to 2 mL TrypLE (Gibco, New York, USA) at 37 °C for 45 min to dissolve cell clot ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed 2 times with pre-warmed PBS (Thermo Scientific, cat. #14190), final PBS wash was replaced with endothelial MV2 basal medium devoid of serum and supplements ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with 4’,6-diamidino-2-phenylindole (DAPI, 62247, ThermoFisher Scientific) for 2 minutes at 1:2000 dilution in 1X PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Other lentivirus cell lines were selected with 2 µg/mL blasticidin (Life Technologies). Cell lines containing SunTag scaffolds were generated from clonal parental lines expressing the desired sfGFP-scFv construct at comparable levels.
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bioRxiv - Neuroscience 2023Quote: ... Cells expressing sgRNAs were then selected for with 2 μg/mL puromycin (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Caco-2 cells were grown in Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Infected cells were treated with 2 μg/ml doxycycline (Thermo Fisher Scientific, BP26535) (D1–D7 ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were carefully washed 2 times with D-PBS (Gibco ref 14190-094) and resuspended in N2B27 medium (Neurobasal Medium (Gibco ref 21103049) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were selected using 2 µg/mL Puromycin (Thermo Fisher Scientific, no. A1113803) during 48-72 h ...
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bioRxiv - Biophysics 2023Quote: The full-length CLC-2 construct was transformed to DH10Bac competent cells (Invitrogen) to isolate baculovirus bacmid ...
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bioRxiv - Immunology 2023Quote: ... Drosophila melanogaster Schneider 2 (S2) cells (Cat#R690-07, Invitrogen, Carlsbad, CA, USA) were transfected with pTLR827–827-PA-His ...
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bioRxiv - Microbiology 2023Quote: ... fixed cells were stained with 4’,6-diamidino-2-phenylindole (DAPI, Thermo Scientific) diluted 1:1000 in phosphate-buffered Saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... cell nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI, 62248, Thermo Scientific) (68) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged every 2-3 days using TryplLE™ express enzyme (GIBCO) and trypsin inhibitor (GIBCO).
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bioRxiv - Molecular Biology 2023Quote: ... HelaS3 cells were treated with 10 µM 5-ethynyl 2’-deoxyuridine (EdU) (Invitrogen) for 15 min to visualize replicated DNA ...
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bioRxiv - Bioengineering 2023Quote: ... cells were maintained in RPMI 1640 medium with B-27 (2%) (Gibco, #17504044) (CM(+) ...
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bioRxiv - Cancer Biology 2023Quote: ... Capan-2 and BxPC-3 cell lines were cultured in RPMI medium (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Physiology 2024Quote: Primary cultured parotid gland cells were loaded with Fura 2-AM (F1221, Invitrogen) (10 μg/ml ...
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bioRxiv - Immunology 2024Quote: ... 2 × 104 cells were seeded in 96-well plates with white walls (Nunc). After 12 h ...
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bioRxiv - Genomics 2024Quote: ... and NTERA-2 cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco, 10566024) as a base ...
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bioRxiv - Biophysics 2024Quote: ... Cells were then centrifuged in a staining buffer with 2% FBS (26140079, Gibco) in PBS ...
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bioRxiv - Immunology 2024Quote: ... cells were transfected with 2 µg of circRNA using Lipofectamine MessengerMax (Invitrogen, #LMRNA003) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... infected cells were selected in growth media containing 2 μg/ml puromycin (Invitrogen). Stable myoblasts were maintained in growth media containing 1 μg/ml puromycin.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were stained with DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) (Thermofisher Scientific) in PBS for 5 min ...
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bioRxiv - Microbiology 2024Quote: ... McCoy cells were washed with 2 mL Hank’s Balanced Salt Solution (HBSS; Gibco), and 1 mL HBSS was added back into each well ...
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bioRxiv - Bioengineering 2021Quote: All experiments used a previously described research cell bank of T-REx-293 human embryonic kidney (HEK) cells stably expressing the tetracycline repressor protein (ThermoFisher) (4) ...
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bioRxiv - Cancer Biology 2020Quote: Cell fractionation experiments were performed using the Subcellular Protein Fractionation Kit for Cultured Cells according to manufacturer’s instructions (Thermo Fisher). Membrane fraction’s protein content was normalized by using anti-transferrin receptor antibody (Invitrogen).
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bioRxiv - Cell Biology 2019Quote: ... The cell pellet was then either processed with the Subcellular Protein Fractionation Kit for Cultured Cells kit (Thermo Scientific, 78840), or resuspended in 1 x RIPA buffer with protease inhibitors to extract whole cell lysate ...
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bioRxiv - Immunology 2022Quote: ... The sgRNA was ordered as modified synthetic sgRNA from Synthego and delivered to HeLa cells by electroporating 200,000 cells with 4 ug TrueCut Cas9 protein (ThermoFisher Scientific) complexed with 180 pmol GBP1 sgRNA using a Neon system (ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Cells were maintained on 6-well plates or cell culture flasks coated with Attachment Factor Protein (Gibco cat. No. S006100) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... Expi293F™ cells (Homo sapiens, embryonic kidney cells in suspension) used for transient protein expression were purchased (ThermoFisher Scientific, A14527) and cultured in Expi293™ Expression Medium (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The transfection complex and BxPC3 cells and PNAC-1 cells were then mixed together and seeded in 6-well (2 x 105 cells/well) cell culture plates (Nunc, Rochester, NY, USA) and cultured at 37°C for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... serum Igs were purified from H10ssF-immunized mice (combined 2-4 weeks after the third immunization) by using protein A/G (Pierce Thermo Scientific). Seven mg of purified heperimmune Igs or control Igs or 100 μg (5 mg kg-1 ...
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bioRxiv - Bioengineering 2022Quote: ... SDS-PAGE analyses were performed by incubating 2 μL of purified protein elution in NuPAGE LDS Sample Buffer (Thermo Fisher Scientific) at 70°C for 10 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... and transfected with 24 µg of plasmid encoding either the wildtype or E406W SARS-CoV-2 spike protein using Lipofectamine 2000 (Life Technologies). Four hours at transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... and subsequently 10 μl protein G Dyna beads were added and incubated for an additional 2 hours (Invitrogen catalogue number 10003D). The samples were washed three times with lysis buffer containing an additional 2% NP40 for 5 min each in a cold room with end-to-end rotation ...
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bioRxiv - Cell Biology 2022Quote: ... were added to 1-2 mg of lysates for overnight incubation at 4 °C before the incubation with protein G Dynabeads (Life Technologies) for additional 2 hrs ...
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bioRxiv - Biochemistry 2019Quote: ... For MS analysis proteins were run for 1-2 cm on a 4-12% PAGE (NuPAGE Bis-Tris Precast Gel, Life Technologies) and stained with silver (SilverQuest Silver Stain ...
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bioRxiv - Cancer Biology 2020Quote: ... The phage library was incubated with biotinylated CD40-Fc fusion protein or Her2 recombinant protein (Acro biosystems) for 2 hours at RT and the phage-antigen complex was captured by Dynabeads M280 (Life technologies). The bound phages were eluted by Glycin-HCl (pH 2.2 ...
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bioRxiv - Immunology 2021Quote: ... was incubated with 20 μg of NKp46-IgG1Fc or NKG2D-IgG1Fc fusion protein at 4° C with rotary agitation for 16 h and then incubated with 2 mM of DTSSP crosslinker (ThermoFisher Scientific) for 2 h on ice ...
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bioRxiv - Microbiology 2021Quote: ... coli cells expressing WT GST-vWA and GST-vWA-C152S mutant proteins were resuspended in PBS supplemented with 2 mM TCEP (Thermo scientific), 0.01 mg/mL lysozyme from chicken egg (SIGMA) ...
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bioRxiv - Biochemistry 2021Quote: The SARS-CoV-2 Omicron HexaPro S protein gene was synthesized and inserted into pcDNA3.1 (GeneArt Gene Synthesis, Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2020Quote: Each gel electrophoresis reaction used 6 µL of unbound B56 protein-antibody complex and 2 µL of 4x SDS-PAGE sample buffer (Thermo Fisher). After heating the samples to 95 C for 10 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... using the Bio-Rad (USA) Protein Assay Dye Reagent Concentrate and a standard curve prepared from a 2 mg/mL bovine albumin standard (Thermo Scientific). The desired protein fractions were pooled ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was run over two immobilized pepsin columns (Trajan, ProDx protease column, PDX.PP01-F32 and Applied Biosystems, Porosyme, 2-3131-00) at 10 °C and 2 °C at 200 μl/min for 3 min ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5-1.0 mg of cell lysate was incubated with 2-4 µg of antibody for 1 h followed by incubation with 8-10 µl of Protein G agarose (Thermo Fisher) for another 30-45 min ...
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bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SCoV-2 proteins were purified by affinity chromatography using the Ni Sepharose® Excel Resin according to manufacturer’s instructions (Thermo Fisher). Protein purity was evaluated by in-gel protein silver-staining using Pierce Silver Stain kit (Thermo Fisher ...