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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for 7 Quinolinecarboxylicacid 1 2 3 4 tetrahydro 1 methyl 2 oxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Coverslips were labeled with 1 μg/mL mAb 13-2 (rOmpB) (53) and 1 μg/mL rabbit polyclonal αTGN46 (Invitrogen, PA5-23068) in PBS overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... siRNA#4: 5’-AUAGCGUUUCUUCUAACUGGGCAGC-3’ (Invitrogen). siRNAs #2 and #4 significantly decreased the mRNA levels to the greatest extent and both had the same mitotic phenotypes ...
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bioRxiv - Microbiology 2021Quote: ... and 1 mM TCEP) with Zeba 7 kDa MWCO spin columns (ThermoFisher), re-quantified by A280 absorbance ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-caprin-1 (Invitrogen, PA5-96857; at a 7 : 10 000 dilution), anti-G3BP-1 (Millipore ...
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bioRxiv - Physiology 2020Quote: ... then 7×106 cells were incubated with CSFE (Thermofisher, C34554, 1:25000) for 20 minutes at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 1/7 of the cytoplasmic fraction was resuspended in TRIZOL (Thermo Fisher). Instead ...
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bioRxiv - Biochemistry 2021Quote: ... and/or ToPro-3-3 (1 μM; Thermo-Fisher Scientific, Waltham, MA) for 10 minutes at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was purchased from Life Technologies/Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and the vesicular stomatitis virus G (VSV-G)-expressing plasmid at a ratio of 7:7:1 (5 μg total amount of DNA) using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 5 mM EDTA and 1% BSA in HBSS) and 2μL of 7-aminoactinomycin D (7-AAD, Thermo Fisher Scientific, A1310) at a concentration of 0.1 mg/mL were added ...
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bioRxiv - Neuroscience 2024Quote: ... The ribosomal pellet was resuspended in 600 µl of 10 mM Tris pH 7 (prepared from 1 M Tris pH 7, Ambion) and stored at –80 °C until all samples for each tissue type were processed until this step.
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bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol)ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N ′ -dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol) ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... HEK 293T/17 (ATCC® CRL-11268) and LLCMK-2 (monkey kidney epithelial cells, ATTC CCL-7) were maintained in Tissue Culture Medium (DMEM (Invitrogen) supplemented with 10% Fetal Bovine Serum (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... and then transferred to nitrocellulose membrane for 7 min at 20 V using the iBlot 2 Dry Blotting System (Invitrogen). Where protein phosphorylation status was investigated ...
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bioRxiv - Bioengineering 2024Quote: Reactive oxygen species (ROS) were quantitatively and qualitatively determined using the cell permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (ThermoFisher Scientific). H2DCFDA is a type of fluorescein that has been chemically reduced and is used to detect the generation of reactive oxygen intermediates in neutrophils and macrophages ...
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bioRxiv - Cell Biology 2023Quote: ... cells were cultured for 7 days in Tu 2% media (1205Lu) supplemented with lipid depleted FBS (Omega Scientific, Fisher Scientific). Dox inducible LBR KD was initiated 3 days prior to the experiment ...
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bioRxiv - Microbiology 2024Quote: ... approximately 5 × 105 Huh-7 cells were transfected with 2 μg of purified BAC DNA using Lipofectamine 3,000 (Fisher Scientific) as a transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then dissociated using 0.5mM EDTA into a single cell suspension and incubated in 5 μM of cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) dye (Invitrogen) for 30 min at 37°C in the dark ...
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bioRxiv - Cell Biology 2023Quote: The ROS dye assay was performed using Di(Acetoxymethyl Ester) (6-Carboxy-2’,7’-Dichlorodihydrofluorescein Diacetate) ROS dye (Invitrogen, D23844), a fluorogenic dye that is converted to 6-CarboxyFluorescein ...
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bioRxiv - Microbiology 2023Quote: ... approximately 5×105 Huh-7 cells were transfected with 2 µg of purified BAC DNA using Lipofectamine 3000 (Fisher Scientific) as a transfection reagent ...
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bioRxiv - Microbiology 2023Quote: ... 7% of heat-inactivated fetal bovine serum (FBS) and 2% of G-418 disulfate 50mg/mL (all from ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: The staining of oxidants was performed with the commercially available 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (#C6827, Thermo Fisher Scientific, USA). Cryo-sections (10 µm ...
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bioRxiv - Bioengineering 2024Quote: COS-7 cells (ATCC, CRL-1651) and HeLa cells (ATCC, CCL-2) were cultured in high-glucose DMEM (Gibco, 21063029) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: A549 cells encapsulated in hydrogels were stained at the beginning (Day 7) and the end of culture (Day 28) with 2 µM Calcein-AM (Invitrogen) and 30 µg/mL propidium iodide (PI ...
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bioRxiv - Plant Biology 2024Quote: Quantification of ROS in guard cells of epidermal peels was measured using 20 µM 2’,7’-dichlorodifluorescein diacetate (H2DCFDA; Invitrogen) according to Pornsiriwong et al ...
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bioRxiv - Neuroscience 2022Quote: ... Star635P anti-guinea pig (Abberior, cat. 2-0112-007-1, 1:100 dilution) and Alexa Fluor 594 anti-rabbit (Thermo Fisher, cat. A-21207, 1:100 dilution), Alexa Fluor 488 anti-chicken (Thermo Fisher ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... FW 5’-CTCTCTGGGCGAAATCTGCT-3’ and REV 5’-GAGTGCTTTCGCTATGTTGTTCA-3’ for Clmn and FW 5’-TGATGGGTGTGAACCACGAG-3’ and REV 5’-GCCGTATTCATTGTCATACCAGG-3’ for Gapdh using a QuantStudio 7 (Applied Biosystems). Transcript levels are expressed as the ratio of 2−ΔCT (Transcript of interest)/2−ΔCT (Gapdh).
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bioRxiv - Biochemistry 2022Quote: ... The complex was eluted by rotating the tubes for 2 hours at 4 °C in elution buffer containing 4 mM SDA (NHS-diazirine, succinimidyl 4,4′-azipentanoate, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... 4% 2-Mercaptoethanol and Bromophenol Blue) and loaded on NuPAGE 4-12% Bis-Tris Gel (catalog #WG1402BOX; Thermo Fisher Scientific). Proteins were transferred on PVDF membrane (catalog #162-0177 ...
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bioRxiv - Bioengineering 2024Quote: ... DAPI (4′-6-diamidino-2-phenylindol) and Myosin Heavy Chain (Myosin 4, eFluor™ 660, Clone: MF20, Affymetrix eBioscience™). All images were taken using a confocal microscope (Zeiss LSM 780 Airyscan ...
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bioRxiv - Cell Biology 2023Quote: ... Day 10 post plating (7 days after air lift) cells were fixed in 4% PFA and stained for tight junction protein ZO-1 (Thermo Fisher Cat# 61-7300). Randomized Z stack images were taken at 20x in 3 different places of each transwell for three transwells and imported into ImageJ ...
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bioRxiv - Cell Biology 2024Quote: ... a premium microscope slide (Fisher-finest, 3″ × 1″ × 1 mm; Thermo Fisher Scientific, 12-544-1) or chambered coverglass (1 well ...
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bioRxiv - Plant Biology 2020Quote: ... 2 µL deoxynucleotides (dNTPs) and 1 µL of RevertAid H Minus-MuLV reverse transcriptase (ThermoFisher Scientific) were added to a final volume of 20 µL ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated for 2 h with anti-rabbit IgG conjugated to Alexa-555 (1:200, Life Technologies) in PBT-BSA at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... fixed tissues were incubated for 2 hours with Alexa Fluor 546 Phalloidin (ThermoFisher Scientific 1:400) in PBS-0.1% Triton X-100 (PT) ...
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bioRxiv - Cell Biology 2020Quote: ... 1-2 µg of cDNA was diluted into 100 µl of Opti-MEM I Medium (Invitrogen) and mixed gently ...
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bioRxiv - Developmental Biology 2020Quote: ... Remaining of the plasmid was digested with 1 μl TURBO DNase (2 U/μl, ThermoFisher Scientific) for 15 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 1 kPa polyacrylamide gels were made using 2 μL of blue fluorescent beads (200 nm; ThermoFisher), 18.8 μL of 40% acrylamide solution (cat no ...
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bioRxiv - Developmental Biology 2022Quote: ... incubated 2 hours in a 1:250 dilution of TRITC-conjugated phalloidin (Molecular Probes, Eugene, OR), and subsequently imaged on a Zeiss LSM 700 confocal microscope.
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bioRxiv - Bioengineering 2019Quote: ... Each device was then washed 1× with PBS and 2× with Neuralbasal™-A medium (Invitrogen), with the last wash applied for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... then probed with a 1:2 000 dilution of primary α-Cas9 antibody (ThermoFisher 10C11-A12) in 5% skim milk-TBST overnight at 4°C ...
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Plant Trans-Golgi Network/Early Endosome pH regulation requires Cation Chloride Cotransporter (CCC1)bioRxiv - Plant Biology 2021Quote: ... plants were germinated in 2 mL of media placed in 1-well microscopy slides (Thermo Fisher) and grown vertically ...
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bioRxiv - Bioengineering 2021Quote: ... and placed into a 2 mL Eppendorf tubes filled with 1 mL of RPMI 1640 (Gibco), 50 µM 2-mercaptoethanol (Sigma) ...
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bioRxiv - Physiology 2021Quote: ... Adipogenic media for days 1-2 contained: DMEM with 4.5 g/L glucose (Thermo Fisher Scientific), 10% calf serum (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... 40 mg tissue sample or 1×105 cultured cells were homogenized in 2 mL Trizol (Invitrogen), and then 1 mL chloroform was added ...
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bioRxiv - Cell Biology 2021Quote: ... 2% chick embryo extract (CEE,E.G.G. Technologies) and 1% (v/v) Penicillin/Streptomycin (P/S, Gibco), and 20 U/ml gamma interferon (γFN ...
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bioRxiv - Systems Biology 2021Quote: ... 1 µL of cells is spotted on a 2 percent low melting agarose pad (Invitrogen 16520050) made with 1X PBS ...
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bioRxiv - Microbiology 2020Quote: ... the spike-in culture was aliquoted in a 1:2 ratio with RNAlater™ solution (Invitrogen) on ice and stored at -80 °C.
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bioRxiv - Systems Biology 2020Quote: ... Non-transduced cells were eliminated by 72 h treatment with 2 μg mL−1 puromycin (Gibco).