Labshake search
Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for 6 FLUORO THIOCHROMAN 3 YLAMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... Hepa 1-6 (Murine hepatoma) cells were grown in DMEM (Gibco; 11995-065) supplemented with 10% heat-inactivated FBS (Gibco ...
-
bioRxiv - Cancer Biology 2023Quote: ... nuclei were stained with DAPI (4’,6-diamino- 2-phenylindole, dihydrochloride, Thermofisher, #D3571) solution (0.5 mg/ml) ...
-
bioRxiv - Microbiology 2023Quote: ... 4 and 6 hpi RNA was extracted from cells using TRIzol (Invitrogen; 15596026). RNA was isolated and precipitated following the manufacturer’s institutions and 200ng was reverse transcribed into cDNA using the ABI cDNA synthesis kit (Applied Biosystems ...
-
bioRxiv - Genetics 2023Quote: ... Taqman reactions were performed on a QuantStudio 6 qPCR machine (Thermo Fisher, #A43168) following manufacturer instructions using ∼25ng of DNA ...
-
bioRxiv - Genetics 2023Quote: ... All samples were run on a QuantStudio 6 qPCR machine (Thermo Fisher, #A43168). CT values were normalized to standard curves generated from the BAC clones ...
-
bioRxiv - Immunology 2023Quote: ... 1 mg/ml Collagenase IV and 6 U/ml DNase I (ThermoFisher Scientific) at 37°C and 750rpm for 30 minutes21,22 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific). The preparations were analyzed under a confocal microscope Zeiss 510 LSM META and Zeiss 780-NLO (Carl Zeiss Microscopy ...
-
bioRxiv - Cell Biology 2023Quote: ... and 6-diamidino-2-phenylindole (DAPI) were obtained from Invitrogen (Carlsbad, CA, USA). General laboratory chemicals and biochemicals ...
-
bioRxiv - Developmental Biology 2023Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2024Quote: ... to which 1 μl of 4′,6-diamidino-2-phenylindole (DAPI) (D1306, Invitrogen) was added ...
-
bioRxiv - Bioengineering 2024Quote: ... Spherical beads (4, 6, 8 and 10 μm diameter) were sourced from Invitrogen.
-
bioRxiv - Genetics 2024Quote: ... germlines were stained with DAPI (4′,6-diamidino-2-phenylindole) (Thermo Fisher Scientific). Germlines were mounted in Vectashield (VectorLabs ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 6 Nav1.5-knock-down MDA-MB-231 tumours) tissue using TRIzol (Invitrogen), according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... for 6 hours at 37°C using Lipofectamine 3000 transfection reagent (Invitrogen, #L3000015) (105) ...
-
bioRxiv - Bioengineering 2024Quote: ... Palmitic acid (Palm) and 5(6)- carboxyfluorescein (FAM) were acquired from Acros Organics. Piperidine ...
-
bioRxiv - Neuroscience 2024Quote: ... Reactions were run on the QuantStudio 6 qPCR machine (Life Technologies, California, USA) and samples were analyzed by the comparative CT method (Λ1Λ1CT) ...
-
bioRxiv - Neuroscience 2024Quote: ... Nuclear stain: 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2ng/ml; Molecular Probes). Sections were imaged digitally using a slide scanner (Olympus VS-120 Slide scanner ...
-
bioRxiv - Biochemistry 2024Quote: ... The samples were analyzed in 6% non-denaturing PAGE with SybrGOLD (S11494, Invitrogen) staining ...
-
bioRxiv - Bioengineering 2024Quote: ... All samples were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; ThermoFisher Scientific) to visualize cell nuclei.
-
bioRxiv - Neuroscience 2024Quote: ... IL-6 serum levels were measured using an ELISA kit (88-7064, Invitrogen). The assay was performed following the manufactureŕs instructions and guidelines ...
-
bioRxiv - Molecular Biology 2024Quote: ... and a QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher Scientific, USA). Primer pairs with an amplification efficiency of 90-110% were selected (Appendix S1) ...
-
bioRxiv - Microbiology 2024Quote: ... France) and DNA was counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen) and polysaccharide content (wheat germ agglutinin labeled with fluorescein ...
-
bioRxiv - Neuroscience 2024Quote: ... ASCs in passage 6 (P6) were seeded in α-MEM medium (Invitrogen, USA) supplemented with with 5% human platelet lysate (hPL ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were stained with DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) (Thermofisher Scientific) in PBS for 5 min ...
-
bioRxiv - Systems Biology 2024Quote: ... EBs were plated on gelatin-coated 6-well plates in DMEM medium (GIBCO) supplemented with 20% (vol/vol ...
-
bioRxiv - Cancer Biology 2021Quote: ... clone IMAGE ID 4977050 was obtained from Source Bioscience and PCR cloned using oligos (Tet2fwd: 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTTAatgccaaatggcagtacagt-3’ and Tet2rev: 5’-GGGGACCACTTTGTACAAGAAAGCTGGGTTtcatacaaatgtgttgtaag-3’) into pDonor221 (Invitrogen Gateway, ThermoFisher) and sequenced ...
-
bioRxiv - Cell Biology 2020Quote: ... or a 1:1 mixture of two siRNA to CHMP2A (5’-CAGGCCGAGAUCAUGGACAUG-3’ and 5’-GAAGAUGAAGAGGAGAGUGAC-3’) using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2020Quote: MiaPaCa-2 cells were seeded in 12-well plates and transfected with 3 µg of DNA (NUPR1-GFP, NUPR1-Flag or control vector) and 3 μL of Lipofectamine 3000 Transfection Reagent (Thermo Fisher Scientific) per well ...
-
bioRxiv - Developmental Biology 2021Quote: ... and the reverse primer containing the Sp6 promoter (5’ ATTTAGGTGACACTATAGCCTCTTCAGTTCCTTCTTCCATC-3’).The aqp1a.1 probe was generated from whole extracted cDNA at 30hpf and amplified with the primers (5’-GTCATGAACGAGCTGAAGAGC-3’) and (5’-GGGTCACTTTGAGGACATCTC-3’),incorporated into the pCR-BluntII-TOPO vector (Invitrogen, 45-0245) (containing both the SP6 and T7 promoters) ...
-
bioRxiv - Developmental Biology 2021Quote: ... were transfected with pPyCAG-(HA)3-Nanog-IRES-Puro and pPyCAG-(Flag)3-Resf1-IRES-Puro using Lipofectamine 3000 (Invitrogen cat. L3000001). In parallel ...
-
bioRxiv - Genomics 2020Quote: ... The 16S rRNA gene was amplified from the extracted genomic DNA using 16S universal primers 27F (5’-AGA GTTTGATCMTGGCTCAG-3’) and 1492R (5’-GGTTACCTTGTTACGACTTC-3’) and sequenced using an automated ABI3730XL capillary DNA sequencer (Applied Biosystems, USA) for taxonomic identification at Cosmo Genetech (Seoul ...
-
bioRxiv - Neuroscience 2021Quote: All females were ovariectomized and primed for 3 days with subcutaneous administration of estradiol benzoate (17-β-Estradiol-3-Benzoate, Fisher Scientific, 2 μg dissolved in sesame oil starting 3 days prior to experiments) ...
-
bioRxiv - Cell Biology 2021Quote: Cross-linking experiments of Sgo11-415 and CPCISB10-280 were performed using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Fisher Scientific) in the presence of N-hydroxysulfosuccinimide (NHS ...
-
bioRxiv - Microbiology 2020Quote: ... 229E-RP reverse primer (5’-CCAACACGGTTGTGACAGTGA-3’) and the TaqMan minor groove binder (MGB) probe 229E-TP (FAM-5’-TCCTGAGGTCAATGCA-3’-NFQ-MGB; Thermo Fisher Scientific), derived from the HCoV 229E membrane protein gene sequence as described previously (Vijgen et al. ...
-
bioRxiv - Microbiology 2021Quote: The mcr-1 gene was amplified from the clinical isolate ESBL20150072 (European Nucleotide Archive, accession number; SAMEA104060671) using primers: 5’-TTTTGGATCCCGGTTTTCGGGCTTTTTA-3’ and 5’-ATATGTCGACTCAGCGGATGAATGCGGT-3’ and Phusion polymerase (Thermo Fisher Scientific). PCR product was digested with BamHI and SalI and ligated into pACYC184 digested with the same enzymes (Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2021Quote: ... PCSK9 mRNA expression was assessed by real-time PCR using PCSK9 primer sets 5’-TGTCTTTGCCCAGAGCATC-3’ and 5’-GTCACTCTGTATGCTGGTGTC-3 (Integrated DNA Technologies) and SYBR Select Master Mix (ThermoFisher, ABI 4472908).
-
bioRxiv - Biochemistry 2022Quote: ... was mixed with 10 nm gold nanoparticles and was applied to the glow discharged surface and blotted at 4 °C and 100% relative humidity for 3 s and a blot force of −3 using the Vitrobot IV System (Thermo Fisher Scientific). Grids were plunged in 100% liquid ethane ...
-
bioRxiv - Genetics 2022Quote: ... 5’-GGCAAGCUGACCCUGAAGUdTdT-3’ / 5’-ACUUCAGGGUCAGCUUGCCdTdT-3’ or with a hsa-miR-34a mimic duplex: 5’-P-UGGCAGUGUCUUAGCUGGUUGUU-3’ / 5’-P-CAAUCAGCAAGUAUACUGCCCUA-3’ according to the protocol for Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific).
-
bioRxiv - Microbiology 2019Quote: ... and then induced to differentiate by culturing for further 3 days in a 3:1 mixture of DMEM and Ham’s F12 media (Invitrogen, Palo Alto, CA) containing FCS (10%) ...
-
bioRxiv - Plant Biology 2021Quote: ... a fragment containing 2.21 kb upstream regulatory region of ELF3 was amplified from Col-0 genomic DNA using primers ELF3Prom-Fw (5’-CACCCTTATAAATAAAATTCC-3’) ELF3Prom-Rv (5’-CACTCACAATTCACAACCTTTTTC-3’) and cloned into the Gateway System (pENTR™ Directional TOPO® Cloning kit, Invitrogen) to obtain the pELF3-TOPO plasmid ...
-
bioRxiv - Neuroscience 2020Quote: ... Secondary neurospheres grown for 3 days (3-4 million cells) were harvested and crosslinked with 0.5% formaldehyde (Thermo Fisher, cat. no. 28908) in 0.1 M DPBS for 5 min at RT with soft agitation ...
-
bioRxiv - Cell Biology 2022Quote: ... The C2C12 cells were transfected with 2 μg plasmids (L-Cas9n-EGFP:R-Cas9n-puro=3:1) by electroporation (1650 v/10 ms/3 pulses) with the Neon Transfection System Kit (Thermo Fisher Scientific). The cells were seeded overnight to 24-well plates containing an antibiotic-free complete mediums ...
-
bioRxiv - Plant Biology 2022Quote: 5’-biotinylated RNA and unmodified RNA of Motif 3 (5’-AAAUCGCCGGAG-3’ 5’-Bio-AAAUCGCCGGAG-3’) were synthesized by Eurofins (Hamburg, Germany) and used for LightShift™ Chemiluminescent RNA EMSA Kit (Thermo Scientific). Total protein was extracted from LL and 10 min LL➔HL-treated plants using extraction buffer (25 mM Tris ...
-
bioRxiv - Microbiology 2021Quote: ... the hypervariable V3 region of the 16S rDNA gene was amplified from 20 ng of DNA using the primers 5′-CCTACGGGAGGCAGCAG-3′ and 5′-ATTACCGCGGCTGCTGG-3′ (Integrated DNA Technologies BVBA, Leuven, Belgium),(18) 1U Platinum® PCR SuperMix High Fidelity (ThermoFisher Scientific) and 10 μM of primer-mix ...
-
bioRxiv - Cancer Biology 2019Quote: ... (5’-CUAUGAACAUAAAGUCUGCTT-3’) or its non-specific control (5’-UUCUCCGAACGUGUCACGUTT-3’) were transfected into cells using Lipofectamine 3000 reagent (Invitrogen, Carlsbad, CA).
-
bioRxiv - Neuroscience 2021Quote: ... Drosophila dSF2 sequence was PCR amplified (dSF2 F 5’-CACCATGGGATCACGCAACGAGTGCCG-3’ and dSF2 R 5’- ATAGTTAGAACGTGAGCGAGACCTGG-3’) was cloned into pEntry-TOPO vector (Thermo Fisher Scientific). The pENTR-dSF2 vector was recombined with Gateway plasmid pTWH (Drosophila Genomics Resource Center ...
-
bioRxiv - Genetics 2021Quote: ... SDS-Page separation was completed by running between 3 and 10μg of total protein on a NuPAGE 3%–8% Tris-acetate gel (Thermo Fisher Scientific Scientific). Next ...
-
bioRxiv - Neuroscience 2022Quote: ... sections were incubated in 0.05 M Tris-HCl buffer (pH 7.6) containing 5 mg 3-3′ diaminobenzidine (Thermo Scientific, TA-060-HDX) per 10 mL buffer and a final concentration of 0.01% hydrogen peroxide for 15 min at RT ...
-
bioRxiv - Developmental Biology 2022Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16C in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3). All embryos were raised in 0.22 μm filtered sea water (FSW ...
-
bioRxiv - Immunology 2022Quote: ... targeting SAMHD1 (sense RNA 5’-GCAGAUAAGUGAACGAGAUTT-3’, antisense RNA 5’-AUCUCGUUCACUUAUCUGCAG-3’) or the negative control #1 siRNA using RNAiMAX (ThermoFisher, 13778-075). 24 h after transfection ...