Labshake search
Citations for Thermo Fisher :
2301 - 2350 of 5645 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... 0.5 M Ethylenediaminetetraacetic acid (EDTA) was purchased from Invitrogen (cat #: 15575020). Dynabeads MyOne Streptavidin T1 were purchased from Thermo Fisher Scientific (cat # ...
-
bioRxiv - Pathology 2023Quote: ... containing 10ul of Dynabeads M-450 tosylactivated (14013, Invitrogen, Waltham, MA). The right kidney was extracted for glomerular isolation and left kidney was extracted for pathological characterization.
-
bioRxiv - Molecular Biology 2023Quote: 4-cells embryos were dejelled into a 0.2 M Tris (Ambion) and 0.2 M DTT (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: 300mg of Dynabeads M-270 Epoxy (ThermoFisher Scientific Catalogue No. 14302D) were resuspended in 10 ml of 0.1 M sodium phosphate buffer pH 7.5 and washed twice using a DynaMag-15 magnet (ThermoFisher Scientific Catalogue No ...
-
bioRxiv - Bioengineering 2023Quote: ... cDNA synthesis was accomplished by using M-MLV reverse transcriptase (Invitrogen). qPCR was carried out to determine the expression levels of selected genes using a StepOne plus real-time PCR system (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... dNTP and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen) were used to synthesize cDNA ...
-
bioRxiv - Microbiology 2023Quote: ... and lysed using M-PERTM buffer (ThermoFisher Scientific, Waltham, MA, USA). Cell debris was pelleted ...
-
bioRxiv - Cancer Biology 2023Quote: ... Dynabeads™ M-280 Sheep Anti-Rabbit IgG (Invitrogen™, 11203D), Dynabeads™ MyOne™ Streptavidin C1 (Invitrogen™ ...
-
bioRxiv - Cell Biology 2023Quote: 0.5 M TCEP (Bond-Breaker™ TCEP Solution, Thermo Fisher Scientific) was diluted to 0.5 mM in 20 mM Tris hydrochloride buffer solution (pH = 7.5 ...
-
bioRxiv - Microbiology 2023Quote: ... this buffer was supplemented with 7 M of urea (Fisher Scientific). After sonication ...
-
bioRxiv - Molecular Biology 2023Quote: ... 100 µL of the washed beads (M-270 Streptavidin Dynabeads, Invitrogen) and 2 mL (or 20% ...
-
bioRxiv - Immunology 2024Quote: ... were coupled to to Dynabeads M-280 streptavidin (Thermo Fisher, 11205D) by incubating 30 min at RT ...
-
bioRxiv - Immunology 2024Quote: ... 50 µL of 0.2 M HCl (Fisher Scientific, H/1111/PB17) and 0.25 M TRIZMA base (Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2024Quote: ... 50 µl of resuspended M-280 Streptavidin Dynabeads (#11205, ThermoFisher Scientific) were added into a fresh 2 ml microcentrifuge tube ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 10 μL of 0.3 M DSG (Thermo Fisher Scientific, A35392) was added to the final concentration of 3 mM for the second cross-linking ...
-
bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesised using an M-MLV Reverse Transcriptase kit (Invitrogen) and 1000 ng RNA was used ...
-
bioRxiv - Biochemistry 2024Quote: ... overnight incubation at 4ºC with M-280 streptavidin beads (Life Technologies) was performed instead of 30min at room temperature ...
-
bioRxiv - Cancer Biology 2024Quote: ... and then lysed in M-Per lysis buffer (Thermo Fisher, 78501) supplemented with 1x Ethylenediaminetetraacetic acid (EDTA ...
-
bioRxiv - Cell Biology 2024Quote: ... 0.05 M Tris-HCl pH 8.0 (Thermo Fisher Scientific, Cat # 15568025), 5 mM MgCl2 (Quality Biological ...
-
bioRxiv - Genomics 2024Quote: ... a dilution of sterile 0.5 M EDTA (Thermo Fisher Scientific; 15575020) in said PBS at a concentration of 0.5 mM was used to detach the cells from the plate ...
-
bioRxiv - Cancer Biology 2021Quote: ... A region spanning 2163 bps upstream from the transcription start site was PCR amplified using genomic DNA isolated from HEK293 cells with Platinum SuperFi II DNA polymerase (Thermo Fisher Scientific). The PCR product was cloned into pGL3 basic vector following conventional protocols ...
-
bioRxiv - Cell Biology 2020Quote: Samples prepared following immunoprecipitation from HEK293 MceC3XFLAG cell line were analysed by LC-MS on an Orbitrap Elite™ mass spectrometer (ThermoFisher Scientific) coupled to a Dionex Ultimate 3000 Ultra-Performance Liquid Chromatography (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... HEK293 Phoenix cells were transfected with the pBabe plasmid DNA encoding for the protein of interest using Lipofectamine 3000 (Thermo Fisher Scientific) per manufacturer’s recommendation ...
-
bioRxiv - Molecular Biology 2019Quote: ... Transfection of Flp-In T-Rex HEK293 cells was carried out according to Flp-In™ T-REx™ Core Kit (Invitrogen). Briefly ...
-
bioRxiv - Cell Biology 2020Quote: HEK293 cells were transfected with pcDNA3.1-GAS6-MycHis linearized with PvuI restriction enzyme using Lipofectamine® 2000 Transfection Reagent (Thermo Fisher Scientific), according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: ... The P2 baculovirus produced in Sf9 cells was added to HEK293 GnTI- cells (mycoplasma test negative, ATCC #CRL-3022) and grown in suspension in FreeStyle medium (GIBCO-Life Technologies) supplemented with 2% FBS at 37°C and 8% CO2 ...
-
bioRxiv - Biochemistry 2020Quote: pcDNA5-based constructs for the expression of C-terminally His6-PreScission protease cleavage site-2xFlag (Flag)-tagged full-length or N-terminally truncated ASCC3 variants were transfected into HEK293 Flp-In™ T-REx™ cells (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... For the preparation of the BGLF2-KO virus, HEK293/EBV(dBGLF2) cells (Konishi et al., 2018) were transfected with BZLF1 expression plasmid using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: Linearized plasmids containing the ItypOrco gene were transfected into HEK293 cells containing a tetracycline-inducible repressor (TREx) using Lipofectamine 2000 (Thermo Fisher Scientific) (Corcoran et al ...
-
bioRxiv - Microbiology 2020Quote: ... HEK293-EBV B(+/−) S13+/− cells were transfected with pCDNA3-BZLF1 and pCDNA3-BALF4 using TurboFect Transfection Reagent (Thermo Scientific, Waltham, MA, USA). Old medium was changed with fresh medium in one day post transfection ...
-
bioRxiv - Immunology 2021Quote: HEK293 adherent cells grown to a confluency of 70–90% were transfected using a Lipofectamine 3000 Reagent Kit (L3000075, Invitrogen, Waltham, MA). Transfection procedures were adapted from the product manual ...
-
bioRxiv - Microbiology 2022Quote: ... Titration of progeny viruses from all HEK293 cells was done in Vero-E6 cells cultured in 16-well chambered slides (Thermo Fisher Scientific), as described below ...
-
bioRxiv - Genetics 2022Quote: ... These plasmids were inserted into the same place in the genome using the Flp-In recombinase technology to generate Flp-In HEK293 (Thermofisher, cat. #R78007) stable cell lines ...
-
bioRxiv - Molecular Biology 2021Quote: HeLa cells and HEK293 cells (in 35-mm dishes) were transfected with 100 pmol siRNA using Lipofectamine RNAi max (Invitrogen–Thermo Fisher Scientific) according to manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2020Quote: HEK293 cells were grown in a monolayer on 10 cm culture dishes in Dulbecco’s Modified Eagle Medium (DMEM) (ThermoFisher Scientific, Waltham, MA) supplemented with fetal bovine serum (10% v/v ...
-
bioRxiv - Molecular Biology 2019Quote: ... Virus-like particles (VLPs) containing either dCas9-KRAB or a targeting sgRNA were generated by transfection of HEK293 cells with Lipofectamine 2000 (Thermo Fisher Scientific). Cells were cotransfected with the packaging plasmid pCMV-dR8.91 ...
-
bioRxiv - Systems Biology 2021Quote: HEK293 cells were maintained at 37°C and 5% CO2 in phenol red-free Dulbecco’s Modified Eagle Medium (Gibco, Thermo Fisher Scientific) supplemented with 10% fetal calf serum (Sigma-Aldrich (MilliporSigma)) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... HEK293 cells were a gift from Dr Harvey McMahon and were cultured in high-glucose DMEM supplemented with GlutaMAX (Thermo Fisher, 31966047) and 10% FBS (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2020Quote: Cell lines were made by co-transfecting plasmids with lentiviral packaging vectors (VSVG and SPAX2) into packaging cells (HEK293-FT) using Lipofectamine 2000 (Thermo Fisher Scientific). Viral supernatants were collected ...
-
bioRxiv - Biophysics 2021Quote: The HEK293 cell line subclone TS201A was a gift from Vladimir Yarov-Yarovoy and was maintained in DMEM (Gibco Cat# 11995-065) with 10% Fetal Bovine Serum (HyClone ...
-
bioRxiv - Neuroscience 2022Quote: HEK293-T cells of low passage (<25) were kept in Dulbecco′s Modified Eagle′s Medium (DMEM, Thermo Fisher Scientific #31966-047) containing 10% fetal calf serum (FCS ...
-
bioRxiv - Biochemistry 2022Quote: ... 12 pmol crRNA:tracrRNA duplex was combined with 12 pmol recombinant HiFi Cas9 (IDT) and reverse transfected into HEK293 Flp-In cells using Lipofectamine CRISPRMAX reagent (Thermo Fisher Scientific) following IDT protocols ...
-
bioRxiv - Genetics 2022Quote: ... pgLAP1-3MYC-BioID2 subnit plasmids were then transformed and stably integrated into HEK293-Flp-In-T-REx cells using the manufacturer’s protocol (ThermoFisher Scientific, catalogue #R78007).
-
bioRxiv - Cell Biology 2022Quote: ... into the Flp-In™ T-REx™ HEK293 cell line or Jaw1 KO cells (#R78007; Thermo Fisher Scientific, Waltham, MA, USA). After incubation for 24 h ...
-
bioRxiv - Physiology 2023Quote: ... mVenus:FL_ Proline and mVenus:2x linker proteins were transfected into HEK293 (Catalog CRL-1573, ATCC) cells with lipofectamine 3000 transfection reagent (Catalog L3000008, Thermo Fisher Scientific). 24h post-transfection ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: Expression vectors of SLC22A10 orthologs were introduced into HEK293 Flp-In cells either through transient transfection or stable transfection using Lipofectamine LTX (Thermo Fisher Scientific). For transfections in a 48-well plate (seeding density ...
-
bioRxiv - Molecular Biology 2023Quote: ... The mCherry tagged SETD6 WT or Y285A and EGFP-tagged E2F1 WT or K117R were co-transfected into HEK293 cells using polyethyleneimine (Thermo Fisher Scientific) according to manufacturer’s instructions ...
-
bioRxiv - Biophysics 2023Quote: S1PR1-Flag and CD69-StrepII were separately expressed using baculovirus-mediated transduction of mammalian HEK293S GnTI− cells (ATCC CRL-3022) in a medium containing FreeStyle 293 (Gibco Cat# 12338018) supplemented with 2% charcoal-dextran stripped fetal bovine serum (Gibco Cat# 12676029) ...
-
bioRxiv - Genetics 2023Quote: ... Lentiviruses were produced transfecting HEK293 cells growing on a T75 flask with 20ug of total DNA and Lipofectamine 2000 (Thermo Fisher Scientific) for 16h ...
-
bioRxiv - Cell Biology 2023Quote: ... pLVX-DsRed-Spartin plasmid together with psPAX2 and VSV-G plasmid were transfected into HEK293 cells using Lipofectamine 3000 (L3000015; Thermo Fisher Scientific) for generating lentivirus ...