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Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for Bcl2 Like Protein 14 BCL2L14 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... recombinant proteins were diluted in EBC buffer and incubated with FAM111A antibody (2 μg/sample) coupled to Protein A agarose beads (Thermo Fisher Scientific) at 4°C for 2 hours on a rotary wheel ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were blocked with 5% milk in TBST buffer and were probed with antibodies diluted in 5% BSA in TBST buffer against the following proteins: FCHSD2 (#PA5-58432, 1:500, Thermo Fisher Scientific), Rab11 (#5589S ...
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bioRxiv - Genetics 2021Quote: ... aliquots of a custom-made rabbit anti-NHE6 antibody (C-terminal epitope: GDHELVIRGTRLVLPMDDSE, Covance #048) (Ouyang et al., 2013) were conjugated to Dynabeads Protein G (Thermo Fisher #10004D) at room temperature for 2 h according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... For E-cadherin and β-catenin IPs extracts were incubated with control or respective antibody (Table S3) together with Dynabeads Protein G magnetic beads (Thermo Fisher Scientific) for 14-16 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were then incubated overnight at 4°C with pan-flavivirus anti-E antibody and protein G Sepharose beads (Thermo Fisher Scientific). Beads were washed three times and eluted with 2× SDS loading buffer before Western blot analysis.
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bioRxiv - Genomics 2020Quote: ... SEMA3C protein levels were checked by western blot as described above using rabbit anti-human SEMA3C antibody (1:1000, Thermo Fisher Scientific). Beta-actin (housekeeping gene ...
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bioRxiv - Molecular Biology 2019Quote: ... and pol μ antibodies were affinity purified from rabbit serum by coupling each full-length recombinant protein to AminoLink Coupling Resin (Thermo Scientific) and following manufacturer’s instructions for IgG purification.
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bioRxiv - Neuroscience 2020Quote: ... Polysomes were immunoprecipitated using monoclonal anti-EGFP antibodies (clones 19C8 and 19F7; see Heiman et al., 200830) bound to biotinylated-Protein L (Pierce; Thermo Fisher Scientific)-coated streptavidin-conjugated magnetic beads (Life Technologies) ...
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bioRxiv - Genetics 2021Quote: ... the same procedure was used except that 20 ug of α-HA antibody (MBL 180-3) was bound to 400 ul equilibrated Protein A agarose (Invitrogen, 15918014) by rotating at room temperature for 1h and washed 3x with extraction buffer and protein was eluted 3x with 300 ul of 1 mg/mL HA peptide (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... The chromatin was incubated with antibodies at 4 °C overnight and then added to 30 μl of protein G-Dyna beads (Thermo Fisher Scientific) for an additional 2 hours with constant rotation ...
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bioRxiv - Cancer Biology 2020Quote: ... Bcl-xL-associated factors were immuno-precipitated using rabbit anti-Bcl-xL antibody (54H6, Cell Signalling Technology) immobilized on the magnetic Protein G Dynabeads (Thermo Fisher Scientific). Normal rabbit IgG (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were washed once with PBS and unspecific binding of antibodies was blocked by Ultravision Protein Block solution (Thermo Fisher Scientific) by a 10 min incubation at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... lysates from 106 cells were immunoprecipitated using 4 µg anti-SLC35A1 antibody and Protein G magnetic Dynabeads (Thermo Scientific, 10-003-D) overnight at 4C prior to immunoblotting to enhance signal and specificity of signal.
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bioRxiv - Physiology 2020Quote: ... as previously described (Danahay et al., 2015) with secondary antibodies to enable fluorescence detection of both proteins (Alexa Fluor; Thermo Fisher, UK). The MUC5AC+ stained area was visualised using a Zeiss Axiovert epifluorescence microscope with a motorised stage that was used to image the same 9 regions of interest on each insert ...
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bioRxiv - Biochemistry 2022Quote: ... Immunoblots were visualized by film exposure using horseradish peroxidase-conjugated secondary antibodies and SuperSignal West Femto (DNMT3A) or SuperSignal West Pico PLUS (other proteins) chemiluminescent substrates (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2022Quote: ... 500 μg of the supernatant was incubated with 2 μg of antibody followed by incubation with 10 μl 50% slurry of Protein A/G agarose (Thermo Fisher Scientific). After extensive washing ...
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bioRxiv - Plant Biology 2022Quote: ... or 5 μg of polyclonal anti-RpoB antibody (PhytoAB catalog number Phy1239) with 40 μl Protein G Dynabeads (Invitrogen catalog number 10004D) or 60 μl Protein A Dynabeads (Invitrogen catalog number 10002D ...
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bioRxiv - Immunology 2022Quote: ... The final antibody concentration was determined by measuring the absorbance at 562 nm using Pierce™ BCA Protein Assay Kit (Thermo Scientific). Purified antibodies were stored at -80°C prior to use.
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bioRxiv - Biochemistry 2020Quote: AMPylated proteins were precipitated from purified recombinant Bip and Bip-AMP or Cho-K1 lysates stressed with either thapsigargin or cycloheximide with antibody 17G6 using Pierce ChIP-grade Protein A/G Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Sodium azide was removed from antibodies before use by diluting in 2 ml PBS and re-concentrating using a protein concentrator column (88521, Thermo Fisher Scientific) as this is critical for live imaging experiments using antibodies (Eilken et al. ...
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bioRxiv - Neuroscience 2021Quote: ... Sheared chromatin was cleared and incubated overnight at 4C with primary antibodies that were pre-incubated with protein G magnetic DynaBeads (Thermo Fisher, 10004D). Primary antibodies used included anti-H3K27Ac (Abcam ...
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bioRxiv - Immunology 2020Quote: ... was used as soluble substrate to detect SARS-CoV-2 S-protein specific monoclonal antibodies and the final reaction was measured by using the Varioskan Lux Reader (Thermo Fisher Scientific) at a wavelength of 405 nm ...
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bioRxiv - Molecular Biology 2019Quote: ... The proteins were eluted with 2X SDS sample loading buffer and analyzed by immunoblotting using anti-myc antibody (Thermo Fisher MA1-980).
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bioRxiv - Molecular Biology 2021Quote: ... Co-purification of 6His-tagged PGC-1α protein domains was detected by western blot using anti-6His antibody (clone His.H8, ThermoFisher Scientific MA1-21315).
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bioRxiv - Synthetic Biology 2020Quote: ... pastoris was confirmed by detecting CSGA-GFP fusion protein using polyclonal rabbit anti-GFP antibodies (from Life Technologies USA; cat # A-11122). Amount of protein was normalized ...
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bioRxiv - Genomics 2021Quote: ... Supernatants were then immunoprecipitated on a rotator for two hours at 4 °C with antibody pre-bound to 17 μl Protein A Dynabeads (Life Technologies 10001D). The following antibodies were used ...
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bioRxiv - Cell Biology 2020Quote: ... Supernantants were used for immunoprecipitation over-night with relevant antibodies or irrelevant IgG bound to Dynabeads Protein G magnetic beads (ThermoFisher Scientific, 10004D). Washes were done 6 times with 1 mL of cold IPH buffer ...
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bioRxiv - Genetics 2021Quote: ... blocked with 5% FBS and then the target proteins were probed with primary and appropriate fluorophore conjugated secondary antibodies (Thermo Fisher Scientific), as previously described (Sabouny et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... Detection of proteins was performed using secondary antibodies conjugated to horseradish peroxidase-HRP and the SuperSignal West Pico Plus chemiluminescent substrate (Thermo Scientific-Pierce).
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates containing 1 mg of protein were incubated with anti-HA antibody (1:100) for 1 h at 4 °C followed by incubation with Protein G Dynabeads (Thermo Fisher Scientific) for 1 h at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... 4 μg of anti-HECTD1 antibody or normal Rabbit IgG was coupled to Dynabeads® magnetic beads protein G (ThermoFisher Scientific, #10003D) for 1 hr at RT ...
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bioRxiv - Biochemistry 2020Quote: ... The chromatin was incubated with antibodies at 4 °C overnight and then added to 30 μl of protein G-Dyna beads (Thermo Fisher Scientific) for an additional 2 hours with constant rotation ...
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bioRxiv - Immunology 2020Quote: His-tagged recombinant mouse CD47 (as well as proteins and antibodies noted later) was labeled using AlexaFluor488 NHS Ester (Succinimidyl Ester, Thermo Fisher Scientific) reconstituted in anhydrous DMSO (dimethylsulfoxide ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: The fluorescence-labeled AFP1-His proteins were prepared according to the manufacturer’s instruction of the DyLight 550 Antibody Labeling kit (Thermo Fisher; Cat#84530). One OD600 of cells washed with MES buffer was subjected to immunostaining using the primary anti-HA antibody and AF488-conjugated secondary antibody or wheat germ agglutinin conjugated antibody (WGA-A488 ...
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bioRxiv - Molecular Biology 2020Quote: ... normalized to 2 mg/ml and protein expression was analysed by western blotting employing anti-V5 antibody (1:3000 dilution, Invitrogen, #R960-25).
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bioRxiv - Immunology 2020Quote: ... was used as soluble substrate to detect SARS-CoV-2 S-protein specific monoclonal antibodies and the final reaction was measured by using the Varioskan Lux Reader (Thermo Fisher Scientific) at a wavelength of 405 nm ...
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bioRxiv - Immunology 2020Quote: ... Detection of proteins was performed using secondary antibodies conjugated to horseradish peroxidase-HRP and the super signal west pico chemiluminescent substrate (Thermo Scientific-Pierce).
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary (4°C overnight) and secondary antibodies (1 h, room temperature) dilutions and proteins were visualized with enhanced chemiluminescence (ECL) (Pierce; Thermo Fisher Scientific). For loading control (Supplementary figures 3b and 3k) ...
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bioRxiv - Immunology 2022Quote: ... VRC07 IgG antibodies were then purified from the culture supernatant using affinity chromatography with Pierce Protein A/G Agarose (Thermo Fisher Scientific) followed by size-exclusion chromatography using a Superdex 200 column (GE Healthcare ...
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bioRxiv - Genetics 2022Quote: ... Blots were blocked for nonspecific protein binding with 5% (w/v) nonfat milk and then incubated with a 1:500-diluted antibody against COL15A1 (Thermo Fisher Scientific) or 1:3000-diluted antibody against GAPDH (glyceraldehyde 3-phosphate dehydrogenase ...
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bioRxiv - Neuroscience 2022Quote: ... For IHC sections were blocked with Protein block for 10 min and incubated with primary antibodies against Aβ (6F3D Amyloid β, Thermo Fisher) diluted 1:100 in PBS + 0.5% goat serum + 0.5% BSA for 1 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... which were supplemented with additional 800 μL of RIPA buffer and incubated overnight at 4°C with 15 μL of antibody-coupled protein G Dynabeads (Thermo Fisher Scientific). Before adding the cleared chromatin ...
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bioRxiv - Molecular Biology 2022Quote: ... was taken before incubating the rest of the supernatant overnight with Protein G Dynabeads and 10 µg anti-GFP antibody (A-11122; Thermo Fisher Scientific). The following day ...
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bioRxiv - Cancer Biology 2024Quote: ... The membrane was then blocked using 5% w/v non-fat dry milk in TBST for one hour at room temperature and then incubated with primary antibody (anti-Alix, 1:5000; Protein Tech, 12422-1-AP or anA-FloAllin-1, 1:5000; Fisher Scientific, BDB610820) at 4°C overnight ...
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Srs2 binding to PCNA and its sumoylation contribute to RPA antagonism during the DNA damage responsebioRxiv - Molecular Biology 2024Quote: ... The activated form of Mec1 was enriched by immunoprecipitating myc-tagged Ddc2 using Protein A beads and anti-Myc antibody (Thermo Fisher 9E10) for 2–4 h at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... 1h at RT and subsequently incubated overnight at 4°C in primary antibody solution using Rabbit anti-Green-Fluorescent-Protein (GFP; 1:1000 ThermoFisher Scientific, A6455). The following day sections were rinsed three times in PBTriton 0.5% and incubated overnight at 4°C in secondary antibody solution using Goat anti-Rabbit-Oregon Green 488 (1:500 Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... The precipitated proteins were visualized by SDS-PAGE and Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific) to determine the protein distribution in each fraction.
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bioRxiv - Cell Biology 2024Quote: ... Expression of the recombinant LZTR1 proteins was confirmed by Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific). Following confirmation of expression ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was immunoprecipitated using 2 μg/ml anti-mouse ST2 antibody and Pierce™ Protein G Magnetic Beads (ThermoFisher, Cat# 88848), followed by western blot analysis.