Labshake search
Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for 7 CHLORO 2 ETHYL 1H INDENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2019Quote: ... The RT-qPCR assays were completed using the ABI ViiA™ 7 sequence detection system (Applied Biosystems®) with the following parameters ...
-
bioRxiv - Microbiology 2019Quote: ... Haematopoietic stem cells differentiated into BMDMs by incubating for 7 days in BMDM medium (1:1 SFM:DMEM, Gibco) supplemented with 10 % FCS (Biochrom) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Each murine sample was analyzed in triplicate on ViiA™ 7 Real-Time PCR System (Applied Biosystems®). Data generated was normalized to β-actin.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and Huh 7 engineering by lentivirus transduction to overexpress the human ACE2 (Huh-7hACE2) were cultured in Dulbecco’s modified 7 Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco). Working stocks of SARS-CoV-2 ICGEB-FVG_5 isolated in Trieste ...
-
bioRxiv - Neuroscience 2021Quote: ... Forty cycles of amplification were run on a Quant Studio 7 Flex Real-Time PCR System (Applied Biosystems) and the resulting data was analyzed using the comparative ΔΔCT method (Livak and Schmittgen ...
-
bioRxiv - Bioengineering 2020Quote: ... WT-PGP1 were labeled with 5 μM CellTracker™ Blue 7-amino-4-chloromethylcoumarin CMAC (Molecular Probes, #C2110), iNeuron-PGP1 was labeled with 2.5 μM CellTracker™ Green CMFDA (Molecular Probes ...
-
bioRxiv - Microbiology 2019Quote: ... at a final DNA concentration of 0.4 ng/μl using the ViiA 7 Real Time PCR System (ThermoFisher). Amplification conditions were as follows ...
-
bioRxiv - Genetics 2021Quote: ... and expression levels were quantified in the QuantStudio(tm) 7 Flex Real-Time PCR machine (Thermo Fisher Scientific) using 2xSybRGreen Master Mix (Applied Biosystems ...
-
bioRxiv - Genetics 2020Quote: ... reduced whole cell lysates were electrophoresed on a NuPAGE 7% Tris-Acetate pre-cast 1.0 mm gels (Invitrogen) for 50 minutes at 150 V and transferred to polyvinylidene difluoride (PVDF ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cos-7 cells were transfected with 2.5μg of hexahistidine (His6) tagged TUBA1A plasmid DNA using Lipofectamine 3000 (Invitrogen). For Cos-7 proteasome inhibition assays ...
-
bioRxiv - Biochemistry 2021Quote: ... ice cold PBS at 2e6 cells/ml and stained with 7-AAD viability dye (1 ug/mL, Invitrogen). Cell sorting was done on a FACSAria III instrument ...
-
bioRxiv - Molecular Biology 2021Quote: ... RNAs were concentrated in an Amicon Ultra 7 kDa filter and buffered exchanged into nuclease free water (Invitrogen), to which the indicated buffer and salt concentrations were added ...
-
bioRxiv - Genomics 2021Quote: ... qPCR was performed on Viia 6/7 Real time PCR system using SYBR Green master mix (Life Technologies). The primers used are listed in Supplementary table 1 ...
-
bioRxiv - Molecular Biology 2021Quote: ... HepG2 and Huh-7 cells were transfected with miRNA or siRNA using LipofectamineTM RNAiMAX transfection reagent (ThermoFisher Scientific) according to the manufacturer’s instructions and following experiments were performed 48 h after transfection ...
-
bioRxiv - Biophysics 2021Quote: ... Cells were finally switched to serum-reduced medium comprising DMEM (HEK293T, COS-7) or FluoroBrite DMEM (Gibco, #A1896702) (HeLa) ...
-
bioRxiv - Cell Biology 2021Quote: ... These experiments were carried out with the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems). Expression of circRNAs was calculated relative to the housekeeping genes 18S or ACTB ...
-
bioRxiv - Cell Biology 2020Quote: ... Enteroids were passaged every 5 to 7 days by incubating in 0.25% trypsin 0.5 mM phosphate buffered saline-ethylenediaminetetraacetic acid (PBS-EDTA, Gibco) solution for 3 minutes at 37°C ...
-
bioRxiv - Immunology 2021Quote: ... Whole bone marrow cultures were then cultured in 10 cm non-tissue culture treated dishes for 7 days in Dulbecco’s modified Eagle’s medium (DMEM) containing 25 mM glucose (Invitrogen) and supplemented with 100 U/ml penicillin and 100 ng/ml streptomycin (Sigma) ...
-
bioRxiv - Immunology 2021Quote: ... bone marrow monocytes were immuno-magnetically isolated and culture for 7 days in Dulbecco’s modified Eagle’s medium (DMEM) containing 25 mM glucose (Invitrogen) and supplemented with 100 U/ml penicillin and 100 ng/ml streptomycin (Sigma) ...
-
bioRxiv - Immunology 2020Quote: ... polyclonal CD8+ T cells were labelled with 20 μM CellTracker™Blue CMAC (7-amino-4-chloromethylcoumarin; Invitrogen) for 20-30 min at 37° C ...
-
bioRxiv - Immunology 2021Quote: ... Real-time qPCR reactions were performed using the QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems).
-
bioRxiv - Immunology 2020Quote: ... splenic NP366-374 TMEM were assessed with CellEvent™ Caspase-3/7 Green Flow Cytometry Assay kit (ThermoFisher). Lung single cells were stained with surface markers then incubated with caspase 3/7 green detection reagent for 30 minutes at 37°C as described in the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... siRNAs (3μl of 10μM conc.) and RNAiMax (7 μl) were mixed separately with 75 μl Optimem (Thermofisher, 31985062). Knockdown was initiated by mixing both siRNA and RNAiMAX suspensions together ...
-
bioRxiv - Cell Biology 2022Quote: WT organoids passage 10 were collected 5-7 days after passaging and digested with TrypLE (Thermo Fisher Scientific) for 20 min at 37 °C ...
-
bioRxiv - Neuroscience 2022Quote: Hippocampal neurons were transfected at 7 days in vitro (DIV) using Lipofectamine 2000 (Thermo Fisher, Cat. no. 11668019). Experiments were carried out after 20-24 hours ...
-
bioRxiv - Cancer Biology 2022Quote: ... with 7 to 14 cycles of PCR using the Phusion High-Fidelity PCR Master Mix (Thermo Fisher Scientific). The ctDNA yield was quantified with the Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Technical replicates for each experimental sample were analyzed in a ViiA 7 Real-Time PCR system (Applied Biosystems) using the standard curve method ...
-
bioRxiv - Developmental Biology 2022Quote: ... together with its IgG2a kappa PE/Cyanine 7-Isotype control (1:500, Thermo Fisher Scientific, 25-4321-81), CD31-APC with human specificity for HUVEC experiments (1:500 ...
-
bioRxiv - Cell Biology 2022Quote: COS-7 cells (ATCC CRL-1651) were cultured in DMEM (5.56 mM glucose, glutaMAX supplement, Thermo Fisher 10567022) supplemented with 10% fetal bovine serum (ThermoFisher 10438-034) ...
-
Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: COS-7 cells were transiently transfected with the relevant DNA constructs using Lipofectamine 2000 (Invitrogen, catalog number 11668). The media was collected ...
-
bioRxiv - Genomics 2022Quote: ... Reactions were carried out on a QuantStudio 7 Flex 384-well Real-Time PCR System (Thermo Fisher Scientific).
-
bioRxiv - Genetics 2022Quote: ... and expression levels were quantified in the QuantStudio™ 7 Flex Real-Time PCR machine (Thermo Fisher Scientific) using 2xSybRGreen Master Mix (Applied Biosystems ...
-
bioRxiv - Genetics 2022Quote: ... and expression levels were quantified in the QuantStudio™ 7 Flex Real-Time PCR machine (Thermo Fisher Scientific) using Power SYBR™ Green PCR Master Mix (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: ... Fractions of 7 to 12 representing the translation level were taken for RNA isolation with TRIzol reagent (Ambion). To perform RNA-seq experiment ...
-
bioRxiv - Systems Biology 2022Quote: ... 4 × 108 cells per biological replicate were used after 7 days of blasticidin selection (10 mg/mL, Gibco), which was 9 days post-infection ...
-
bioRxiv - Microbiology 2022Quote: ... cDNA was synthesized from 7 µl of RNA using the High-Capacity RNA-to-cDNA Kit (Thermo Fisher). TaqMan assays (Thermo Fisher ...
-
bioRxiv - Neuroscience 2022Quote: ... was added to each well and amplification continued in a QuantStudio 7 Flex qPCR machine (Thermo Fisher Scientific) until signal plateaued out of exponential amplification using the following programme ...
-
bioRxiv - Microbiology 2022Quote: ... Real-time PCR assays were conducted using a QuantStudio 7 Pro Real Time PCR system from Thermo Fisher Scientific Inc ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... we ran two technical replicate reactions in an QuantStudioTM 7 Flex Real-Time PCR System (Applied Biosystems™). We used 4μL of genomic template ...
-
bioRxiv - Developmental Biology 2022Quote: ... Stained cell suspension was passed through a cell strainer and 7-aminoactinomycin D (Molecular probes by Life Technologies) was added for dead cell identification ...
-
bioRxiv - Developmental Biology 2022Quote: ... Stained cell suspension was passed through a cell strainer and 7-aminoactinomycin D (Molecular probes by Life Technologies) was added for dead cell identification ...
-
bioRxiv - Neuroscience 2024Quote: ... 1.5 μL of cDNA from each sample was run in duplicate using a QuantStudio 7 Flex (Thermo Fisher) and analyzed with the 2-ΔΔCT method.
-
bioRxiv - Plant Biology 2024Quote: ... Data was analysed with QuantStudio 6 and 7 Pro Real-Time PCR Systems Software (Thermo Fisher Scientific, America). For RT-qPCR ...
-
bioRxiv - Cell Biology 2024Quote: ... Treated and untreated (PBS) organoids were collected at day 7 and dissociated to single cells using TrypLE (Invitrogen) incubation at 37°C ...
-
bioRxiv - Neuroscience 2024Quote: ... Transcripts were quantified by real-time quantitative PCR on a Quantstudio 7 Real-Time PCR instrument (Thermo Fisher). Probes for 24 cryptic splice variants (and the respective canonical variants ...
-
bioRxiv - Cell Biology 2024Quote: COS-7 cells (ATCC CRL-1651) were cultured in DMEM (5.56 mM glucose, glutaMAX supplement, Thermo Fisher 10567022) supplemented with 10% fetal bovine serum (ThermoFisher 10438-034) ...
-
bioRxiv - Cell Biology 2024Quote: ... HeLa and COS-7 cells were seeded onto dishes coated with 5 mg fibronectin (Life Technologies 33016-015) in diH2O ...
-
bioRxiv - Cell Biology 2024Quote: ... Z-stack images were taking on day 6-7 after seeding using EVOS™ 7000 Imaging System (Invitrogen). and used for quantification of organoids.
-
bioRxiv - Molecular Biology 2022Quote: ... Quantitative PCR (qPCR) reactions were performed with SYBR green (CWBiotech, CW2621) on the QuantStudio 7 Pro (Applied Biosystems) qPCR instrument ...
-
bioRxiv - Immunology 2023Quote: ... Cells were differentiated for 7 days in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034) supplemented with 5% heat-inactivated fetal bovine serum (Biosera FB-1001 ...