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Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for 6 OXO 9α 11α 15S TRIHYDROXY PROSTA 13E 17Z DIEN 1 OIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... an additional cleaning step with acid phenol was added before precipitation of the RNA by adding an equal volume of Acid-Phenol:Chloroform 5:1 solution pH 4.5 (Ambion). Total RNA was quantified with a Nanodrop 2000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Pathology 2020Quote: ... Total ribonucleic acid of 1 μg was used for the first-strand complementary DNA synthesis (Thermo Fisher Scientific). Quantitative real-time polymerase chain reaction was performed by using Quant Studio 3 (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... the cells were suspended in FacsMax (amsbio) and stained with SytoxTM green nucleic acid stain (1:100000, Invitrogen) and Hoechst (1:5000 ...
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes, and fixed with methanol:glacial acetic acid, (5:1, v/v) (FisherThermo Fisher Scientific, Rockford, IL). Cells were dropped onto slides ...
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bioRxiv - Systems Biology 2020Quote: ... Combined eluates were acidified with trifluoroacetic acid at final concentration of 1% (TFA, mass spec grade, Thermo Fisher) and desalted with a C18 stage tip.
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bioRxiv - Genetics 2020Quote: ... and in some cases day 5 (120 hours) by staining with SYBR Green 1 nucleic acid stain (Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... 1.2% SDS, 12 mM EDTA) and 700 µl hot acid-phenol:chloroform pH 4.5 (with IAA, 125:24:1) (Ambion). After incubating at 65°C for 30 min with shaking at 1500 rpm ...
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bioRxiv - Physiology 2019Quote: ... The pH of the 1% acetic acid solution measured 2.6 using an Accumet AE150 pH probe (Fisher Scientific). The estimated pH once applied to the airway surface is ∼6.6 to 6.8 (22) ...
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bioRxiv - Microbiology 2020Quote: ... Two additional experimental conditions were added: 1) uninfected skin with 10 µl 100% oleic acid (Thermo-Fisher Scientific) applied to the surface of the skin explant and 2 ...
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bioRxiv - Cell Biology 2022Quote: ... worms were transferred to bacteria-seeded plates containing 1 mM indole-3-acetic acid (Acros Organics, Cat #122160250) and incubated for the indicated time periods before analysis.
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bioRxiv - Immunology 2023Quote: ... These cells were cultured in the MEM medium supplemented with 10% FBS and 1% nonessential amino acids (Gibco).
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bioRxiv - Systems Biology 2024Quote: ... 1 μL of GlycoBlue (to improve pellet visualisation) and 400 μL of Acid-Phenol:Chloroform (pH 4.5; Thermo Fisher) were added ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 10% Hi-FBS (Thermo Fisher Scientific). BSC-1 cells (ATTC ...
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bioRxiv - Plant Biology 2023Quote: ... mVenus was cloned solely or with an open reading frame of SUF4 isoform 1 (NP_564369.1; 367 amino acid) in the modified pFastBac vector (ThermoFisher). Site-directed mutagenesis was carried out to create the 5S and 12S mutants using PrimeSTAR Max DNA polymerase (Takara-bio) ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then vacuum dried and resuspended in 50 μL of 1% formic acid (Thermo Fisher Scientific, 695076). Sample peptides were calculated via CBQCA quantification kit (Thermo Fisher ...
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bioRxiv - Biochemistry 2024Quote: ... the gel was stained with 1× SYBR Gold Nucleic Acid Gel Stain for 30 min (Thermo Fisher Scientific). The gel image was quantified by ImageJ (33) ...
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bioRxiv - Bioengineering 2021Quote: ... or a long-chain fatty acid (Hexadecanoic Acid, C16; BODIPY™ FL C16, D3821, Thermo Fisher Scientific Inc.) were added at a concentration of 4 µM to the culture medium as defined by culture mode (table 3) ...
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bioRxiv - Microbiology 2020Quote: ... total nucleic acid was extracted from tissues using the MELT™ Total Nucleic Acid Isolation System (Ambion, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 0.8 mM KH2PO4, 20 mM NaHCO3, 1.3 mM L-glutamine, 0.2 mM ascorbic acid, MEM amino acids solution [Gibco; Thermo Fisher ...
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bioRxiv - Pathology 2023Quote: ... Dilutions of SCFAs standards (Acetic acid:A6283, Sigma-Aldrich; Propionic acid: 81,910, Sigma-Aldrich; Butyric acid:108,111,000, Acros Organics) were prepared in saline solution at concentrations ranging from 87 to 0.087 mM for acetic acid ...
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bioRxiv - Molecular Biology 2024Quote: ... omitting all amino acids without the addition of non-essential amino acids and substitution with dialyzed FBS (Invitrogen). Doxycycline treatment of cells stably expressing ER-phagy probe was performed as previously described7.
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bioRxiv - Cell Biology 2020Quote: ... Ovaries were washed with PBST for 15 minutes before staining nuclei with TO-PRO-3 (Life technologies, 1:1000) in PBST for 15 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... Unbound antibodies were removed in four changes of PBS /0.1% Triton X-100 (15 min each) and nuclei were stained in 1:10000 dilution of 10.9 mM DAPI (Invitrogen) for 30 min ...
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bioRxiv - Biophysics 2022Quote: ... Oocytes were injected with 0.1 to 10 ng cRNA of mASIC1a (volumes between 9 and 50 nL) and incubated for 1–4 days at 18 °C in Leibovitz’s L-15 medium (Gibco), supplemented with 3 mM L-glutamine ...
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bioRxiv - Microbiology 2021Quote: ... followed by 40 cycles at 95 °C (15 s) alternating with 60 °C (1 min) (Applied Biosystems, Carlsbad, CA). Gene-specific products were normalized to a group of 5 housekeeping genes (ACTB ...
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bioRxiv - Bioengineering 2021Quote: ... Gels were run for 1 hour and 15 min at 120 V in with NuPage MES Buffer (NP0002, ThermoFisher). Proteins were then transferred onto polyvinylidene difluoride membrane (IPVH15150 ...
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bioRxiv - Microbiology 2020Quote: ... Purified GST-Pak1PBD (20 μg) was added to 1 ml of 1.0 mg/ml lysates along with 15 μl of glutathione resin (ThermoFisher) and rotated for 90 min at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... Samples were heated at 70 °C for 15 min with 1 x Bolt LDS sample buffer (ThermoFisher Scientific, UK) and 200 mM DTT ...
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bioRxiv - Cell Biology 2020Quote: ... for 15 min at room temperature and blocked in PBS containing 1% bovine serum albumin (BSA, Thermo Fisher Scientific), 2% normal donkey serum (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated for 15 min at room temperature with 1-Step Turbo TMB-ELISA Substrate Solution (Thermo Fisher), then terminated with equal volume of 1M phosphoric acid ...
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bioRxiv - Plant Biology 2021Quote: ... inflorescences were dissected with a hypodermic syringe and embedded in 15% acrylamide:bysacrilamide (29:1) over polysine slides (Thermo Scientific). Slides were treated with 150 μL of 0.2 M HCl for 20 min at 25°C and subsequently washed three times ...
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bioRxiv - Biochemistry 2019Quote: ... cells were grown to confluency in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 15% Fetal Bovine Serum (FBS) and 1% penicillin-streptomycin (Invitrogen) and harvested by trypsinization ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 15% FBS and Eagle’s Minimal Essential Medium (ENEM) supplemented with 1% NEAA (Gibco, Thermo Fisher Scientific Inc.). For miR-124-3p inhibition experiments in SK-N-BE(2 ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 15% FBS and Eagle’s Minimal Essential Medium (ENEM) supplemented with 1% NEAA (Gibco, Thermo Fisher Scientific Inc.). For miR-124-3p inhibition experiments in SK-N-BE(2 ...
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bioRxiv - Cell Biology 2022Quote: ... Ovaries were washed with PBST for 15 minutes before staining nuclei with TO-PRO-3 (Life technologies, 1:1000) in PBST for 15 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 3x in 0.3% Triton X-100 in PBS for 15 mins/wash before incubating in DAPI solution (1:2000 DAPI, Invitrogen, #D1306 ...
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bioRxiv - Molecular Biology 2022Quote: ... to a final concentration of 1% at RT for 15 min and quenched by addition of glycine (ThermoFisher, AJA1083) to a final concentration of 150 mM for 5 min at RT ...
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bioRxiv - Biochemistry 2022Quote: ... and PV VLPs were purified from clarified supernatants by concentration through a 30 % sucrose cushion followed by a 15-45 % sucrose gradient in 1 × Dulbecco’s phosphate-buffered saline (DPBS, Gibco), 20 mM EDTA pH 7.0 (DPBS-EDTA ...
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bioRxiv - Genetics 2023Quote: ... the membranes were washed three times for 15 min each and the appropriate Horseradish peroxidase (HRP) coupled secondary antibody (1:10,000) was added (ThermoFisher). For signal development ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 15% FBS and Eagle’s Minimal Essential Medium (ENEM) supplemented with 1% NEAA (Gibco, Thermo Fisher Scientific Inc.). SK-N-BE(2 ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 15% FBS and Eagle’s Minimal Essential Medium (ENEM) supplemented with 1% NEAA (Gibco, Thermo Fisher Scientific Inc.). SK-N-BE(2 ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... Cells were then incubated for 15 min in 0.01% buffer B and a 1/10,000 dilution of DAPI (Life Technologies) for nuclei staining ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were heated at 80 °C for 15 min with 1 x Bolt LDS sample buffer (ThermoFisher Scientific, UK) and 200 mM DTT ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% Fetal Bovine Serum (BioWest, cat# S1620) and 1% Penicillin/Streptomycin (Fisher Scientific, cat#15-140-122) unless otherwise noted ...
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bioRxiv - Biochemistry 2024Quote: To perform DSF, 5 µM protein was mixed with substrate (0, 1, or 15 mM) and 2.5x SYPRO Orange dye (ThermoFisher), and made up to a total volume of 50 µl with DSF buffer (50 mM Tris pH 8 ...
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bioRxiv - Bioengineering 2020Quote: ... the ADSC and the HUVEC were added after trypsin detachment (both always used at passages 1-6) and centrifugation 1 minute at 3,500 rpm (Minispin, ThermoFisher Scientific, Whaltam, MA, USA) to get a final cell concentration of 4×106 ADSC/mL and 2×106 HUVEC/mL ...
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bioRxiv - Plant Biology 2021Quote: ... The liquid syringe was washed 6 times with each of 10 µL of 1:1 (v/v) acetone:hexane and HPLC grade methanol (Fisher Scientific Co, Edmonton Alberta) both before and after the liquid injection.
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bioRxiv - Evolutionary Biology 2021Quote: ... and the resulting 15 uL mixture was separated by gel electrophoresis with a 15% polyacrylamide TBE-urea gel (Invitrogen EC68852BOX) at 200 V for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... 60°C for 15 seconds and 72°C for 15 seconds using the ProFlexTM PCR System Thermal Cycler (Applied Biosystems). Subsequently ...
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bioRxiv - Developmental Biology 2019Quote: ... They were then washed twice for 15 minutes in PBT and once for 15 minutes in PBS before mounting in Prolong Gold Antifade with DAPI (Invitrogen). For phalloidin staining ...