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Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for 6 Cyano imidazo 1 2 a pyridine 2 carboxylic acid ethyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... T cells were cultured at 2×106 cells/ml in T cell media (R10 supplemented with 1X non-essential amino acids (Gibco) + 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... the samples were re-suspended in 2 % formic acid and analysed using an UltiMate™ 3000 RSLCnano System (ThermoFisher Scientific) coupled on-line to an Orbitrap Exploris 480 (ThermoFischer Scientific) ...
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bioRxiv - Genetics 2022Quote: ... The peptides were dissolved in 2% formic acid and analysed on a Q-Exactive HF mass spectroscopy (Thermo Fisher Scientific). The peptides were separated using an EASY-Spray PepMap RSLC C18 column at flow rate of 300 nL/minute on a 60 min linear gradient of the mobile phase (5% acetonitrile (ACN ...
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bioRxiv - Microbiology 2021Quote: Multiplexed peptide fractions from each time point were resuspended in mobile phase A solvent (2% acetonitrile and 0.1% formic acid) to be analyzed on the Exploris 480 mass spectrometer equipped with FAIMS (ThermoFisher Scientific). The mass spectrometer was interfaced to the Easy nanoLC1200 HPLC system (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2019Quote: Samples were diluted in culture medium (RPMI 1640 [Gibco, Massachusetts/USA] supplemented with 2 g of NaHCO3, 10 mL of nonessential amino acids [Gibco] ...
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bioRxiv - Biochemistry 2020Quote: ... The liquid chromatography used a 2-90% MeCN linear gradient in water with 0.1% formic acid on a C18 BioBasic analytical HPLC column (Thermo Scientific) installed on an Agilent 1290 HPLC system ...
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bioRxiv - Biochemistry 2021Quote: We monitored the change in absorbance over time of 5,5’-dithiobis-(2-nitrobenzoic acid) (DTNB, Ellman’s reagent, Thermo Fisher Scientific). Various concentrations of DTNB were added to protein and change in absorbance was measured in either an SX-20 Stopped Flow instrument (Applied Photophysics ...
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bioRxiv - Immunology 2020Quote: ... the desalted samples were first resuspended into 20 μl 0.1% formic acid in water and then loaded onto a trap column (2 cm × 300 μm, PepMap C18, Thermo Scientific) and an analytical column (19 cm × 75 μm ...
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bioRxiv - Molecular Biology 2020Quote: ... peptides were trapped at 3 μl/min on a 10 mm × 75 μm ID Acclaim Pepmap trap at 2% buffer B (buffer A: 0.1% formic acid (Fisher Scientific), buffer B ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were then dissolved in 2% ACN and 0.1% formic acid and analyzed on a Thermo Scientific Q Exactive Mass Spectrometer (ThermoFisher Scientific). The MS was operated in data-dependent mode ...
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bioRxiv - Molecular Biology 2022Quote: The reconstituted peptides in solvent A (2% acetonitrile, 0.1% formic acid) were separated on a 50 cm long EASY-spray column (ThermoFisher Scientific) connected to an Ultimate-3000 nano-LC system (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% formic acid in acetonitrile) on an EASY-Spray column ES801 (50 µm ID × 15 cm, 2 µm; Thermo Scientific) mounted on a DPV ion source (New Objective ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... Cells were trypsinised and resuspended in FACS buffer (1ξ Ca2+ and Mg2+ free PBS, 2 mM ethylenediaminetetraacetic acid (Life Technologies), 2% FBS Bovogen) ...
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bioRxiv - Cancer Biology 2024Quote: Peptides were reconstituted with 2% formic acid and analyzed in triplicates on an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific) coupled to an UltiMate 3000 UHPLC system (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were resuspended in 2% acetonitrile in 0.1% formic acid and loaded at 5 μL/minute onto a PepMap C18 trap column (Thermo Scientific; 5 μm 100 Å particle size ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µM × 250 mm) in Solvent A (98% water, 2%acetonitrile, 0.1% formic acid) via a Nanospray Flex Ion-Source (Thermo Scientific) at a voltage of 1.9 kV with a flow rate of 0.7 µL/min in Solvent A (98% water/2%acetonitrile/0.1% formic acid) ...
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bioRxiv - Physiology 2024Quote: ... fractions were resuspended in 2% acetonitrile and 0.1% formic acid and separated on an Easy nLC 1200 (Thermo Fisher Scientific) and a 35 cm long ...
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bioRxiv - Microbiology 2022Quote: ... purified CD4+ T cells were maintained for two days in complete RPMI 1640 medium containing recombinant interleukin-2 (IL-2) (300 IU/ml) and phytohemagglutinin (1 μg/ml, Thermo Fisher Scientific), followed by 3 days in the presence of IL-2 alone ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% PFA for 30 minutes and stained for intracellular ENV with 1A1D-2 (1/500) monoclonal antibody and anti-mouse 647-conjugated antibody (2 μg/ml, Invitrogen Cat# A21235).
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bioRxiv - Immunology 2020Quote: ... for 2 days, then passaged 1:2 and cultured overnight in R10 medium (1X Roswell Park Memorial Institute (RPMI, (Life Technologies SAS), 10% fetal calf serum ...
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bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 5 mM DTT, 50 mM HEPES, 3 mM MgCl2, 2 mM PMSF, 1 Pierce™ protease inhibitor mini tablet/2 L; Thermo Scientific). The lysate was sonicated then incubated with 500 units/1 L culture Benzonase Nuclease (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ICabs A4 and B7 were labelled with NHS-Rhodamine (5/6-carboxy-tetramethyl-rhodamine succinimidyl ester, ThermoFisher Scientific (catalogue no. 46406)) as described by the manufacturer ...
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bioRxiv - Biophysics 2022Quote: ... BODIPY FL NHS Ester (Succinimidyl Ester) (NHS-BODIPY; catalog number: D2184) were purchased from Invitrogen.
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bioRxiv - Pathology 2021Quote: RNA samples containing residual DNA contaminants were further treated with 2 μL of buffer (10×) and 2 μL of TurboTM DNAse (2 U/μl) (Ambion®). Samples were incubated at 37°C for 30 min followed by DNAse inactivation by adding 2 μL of DNAse Inactivation Reagent (Ambion®) ...
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bioRxiv - Neuroscience 2020Quote: ... serum-free medium (DMEM:F12 at 2:1) supplemented with N2 (1% v/v; Invitrogen), 1mM L-glutamine ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibody (2-3% serum, 0.4% PBST, 1:500 Invitrogen A11035, 1:500 DAPI) was applied and incubated for one hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 2% B27 and 1% L-glutamine and 1% Pen-Strep (Life Technologies). Dissociated wildtype and Ncan KO cortical neurons were initially infected with Control and the two Ncan shRNA AAVs at 7 days in vitro (DIV ...
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bioRxiv - Biochemistry 2024Quote: ... Solutions were further diluted to 1 µM using 2:1 MeOH:CHCl3 (ThermoFisher Scientific, Australia) with 5 mM ammonium acetate (SigmaAldrich ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 2% B27 and 1% L-glutamine and 1% Pen-Strep (Life Technologies). Dissociated cortical neurons were infected with Control and Ncan shRNA AAVs at 7 days in vitro (DIV ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa647 ICAM-2 at 1:100 and Live/Dead at 1:1000 (L34962, Invitrogen), for sorting of CD45neg ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM 2-mercaptoethanol and 1 mM PMSF) by sonication (using a Fisher Scientific Sonic Dismembrator with a Qsonica Ultrasonic Sonicator converter ...
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bioRxiv - Cancer Biology 2024Quote: ... at a 2:1:1 ratio using Lipofectamine 3000 transfection reagents (Invitrogen, Cat. L3000015). Viral supernatants were collected 48 hours after transfection and filtered through a 0.45-μm membrane (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then dipped in the secondary antibody-containing buffer with the nuclear dye 4′,6-diamidino-2-phenylindole (DAPI, Cat# D1306, Life Technologies-Invitrogen, Carlsbad, CA, USA, dilution 1:400), for 2 hours at RT ...
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bioRxiv - Biochemistry 2020Quote: Beads were first activated with 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (Thermo Fisher Scientific) in the presence of N-hydroxysuccinimide (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) was obtained from Life Technologies (Carlsbad, CA) and N-hydroxysulfosuccinimide (NHSS ...
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bioRxiv - Biophysics 2024Quote: ... and 250 µM of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) (Thermo Scientific, 24510) in Borate buffer (100 mM Borate-NaOH ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed twice with 1X PBS and stained with either DAPI (4’,6-diamidino-2-phenylindole, from Life Technologies), for the visualization of the nucleus ...
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bioRxiv - Cell Biology 2020Quote: We seeded 2×105 cells / well into 6 well dishes and replaced media with 5% KSR (Knockout Serum Replacement) (Gibco) containing media the next day ...
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bioRxiv - Cell Biology 2020Quote: ... collected into a new tube and stained with 4′,6-diamidino-2-phenylindole (DAPI) (∼5 µg/ml, Molecular Probes # D1306). Round coverslips (Ø12 mm ...
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bioRxiv - Genetics 2021Quote: Live myofibers in the 6-well plate were stained with 2 µL of 5mg/mL of Hoechst (Molecular Probes, H1399) in 2 mL of 1X PBS for 5 minutes in a 37 °C with 5% CO2 incubator ...
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bioRxiv - Microbiology 2021Quote: ... Slides were washed for 3 × 5 mins in PBS after each antibody step and in the penultimate wash 2μg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes) was added ...
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bioRxiv - Microbiology 2019Quote: ... at room temperature for 60 min and washed again with PBS-T and then incubated with DAPI (4’, 6-diamidino-2-phenylindole) (Invitrogen) at room temperature for 20 min ...
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bioRxiv - Cancer Biology 2019Quote: 4T1 and 67NR cells at 70-80% confluence were transfected with the shRNA constructs described above in FuGene 6 at a ratio of 3:2 per manufacturer’s instructions (Thermo Fisher). Pools of transfected cells were selected in media with 7 µg/ml puromycin ...
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bioRxiv - Cell Biology 2019Quote: ... Nuclei were stained with 10.9 μM 4⍰6-diamidino-2-phenylindole (DAPI) and cover glasses were mounted in ProlongGold (Life Technologies). Images were collected on an inverted epifluorescence microscope (Nikon TE2000E ...
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bioRxiv - Neuroscience 2019Quote: ... proliferating NSCs were plated on matrigel-coated 6- or 12-well plates and cultured in Neurobasal medium supplemented with B27 without vitamin A (2%, ThermoFisher), PenStrep (1% ...
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bioRxiv - Neuroscience 2020Quote: ... The coverslips were then mounted on slides using prolong Gold mounting medium with DAPI (4’,6-diamidino-2-phenylindole) (Invitrogen). RGCs immunohistochemistry was performed as described earlier 36–39 using SOX2 ...
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bioRxiv - Bioengineering 2020Quote: ... Primary fibroblast passages 2–6 were achieved in culture in 37°C in a 5% CO2 incubator with DMEM High Glucose (Gibco) with 10% FBS to 80% confluence in 100 mm Petri dishes ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed 5-6 times over 2 hours in PBT at room temperature and incubated with secondary antibodies (Invitrogen) diluted in NGS-PBT for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for two hours at room temperature followed by 0.5 μg/mL of 4′,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific) in PBS for five minutes at room temperature prior to mounting.