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Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for 6' ETHYL SPIRO 1 3 DIOXANE 2 3' INDOLIN 2' ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... 1 h with oxygenated water 3% (Tyramide Superboost Kit, B40922, Thermo Fisher, USA) and ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were split 1:10 every 3 days by using trypsin (Gibco, 25200072).
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bioRxiv - Neuroscience 2022Quote: ... Cell nuclei were detected with TO-PRO-3 (1:400; Thermo Fisher Scientific). To analyze PNN structure and to detect synaptic proteins ...
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bioRxiv - Neuroscience 2021Quote: ... samples were then incubated with TO-PRO-3 (Thermo Fisher, T3605, 1:300), goat anti-rat Alexa Fluor 568 (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... A final incubation of 3 min with DAPI (1:1000, Thermo Fisher Scientific) was done ...
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bioRxiv - Microbiology 2021Quote: ... followed by DNA staining with 1 µM TOPRO-3 iodide (642/661; Invitrogen) and staining of the fungal hyphae with 100 μg/ml Fluorescent Brightener 28 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... slides were treated with DAPI (1:500, 3 min RT, ThermoFisher Scientific, D1306), washed briefly in PBS (2x) ...
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bioRxiv - Genetics 2021Quote: ... Nuclei were stained with TO-PRO-3 (Topro, Invitrogen, T3605; 1:3000 dilution).
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bioRxiv - Pathology 2021Quote: ... Membranes were blocked for 1 hour at RT with 3% BSA (Thermo Fisher) in tris-buffered saline with 0,1% Tween (TBS-T ...
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bioRxiv - Developmental Biology 2021Quote: ... and cultured in a 3:1 mixture of IMDM (ThermoFisher Scientific Cat. 12440053) and Ham’s F12 (ThermoFisher Scientific Cat ...
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bioRxiv - Microbiology 2021Quote: ... and primer sequences (Table 1) on a QuantStudio 3 platform (Applied Biosystems, USA). The relative expression levels of target genes were plotted as fold change compared with untreated or negative controls ...
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bioRxiv - Immunology 2020Quote: ... 100 μl) mixed with Alum (1:3 v/v) (ThermoFisher, Waltham, MA, USA). HBc-S was used as a control ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 mM 3’-d-GTP [>99% purity for UTP and CTP (ThermoFisher); ≥95% purity for 3’-dGTP (Jena Bioscience)] and incubated 2 min at 37°C (to “chase,” and thereby to stabilize ...
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bioRxiv - Biophysics 2022Quote: ... Cells were split 1:10 every 3 days by using trypsin (Gibco, 25200072).
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bioRxiv - Cancer Biology 2022Quote: ... incubated for 3 minutes in Hoechst nuclear stain (1:1000 in H2O, Thermofisher), and mounted with Vectashield (VectorLaboratories ...
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bioRxiv - Synthetic Biology 2022Quote: ... for assembly into levels 1 and 3 or 10 U BpiI (Thermo Scientific) for assembly into levels 2 in 20 μl 1x T4 ligase buffer (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... or streptavidin-HRP (1:1000 dilution, Fisher Scientific, Novex Cat# 43-423-3) to ensure that biotinylated and bait proteins were present ...
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bioRxiv - Cell Biology 2023Quote: ... DNA and lipofectamine (1:3 w/v) were diluted in Opti-MEM (Gibco) and incubated at room temperature (RT ...
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bioRxiv - Molecular Biology 2023Quote: ... and primary antibodies for 3 days: chicken anti-GFP (1:750, Thermo Fisher Scientific Cat# A10262 ...
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bioRxiv - Immunology 2023Quote: ... for 3 h and goat anti-rat AF 633 (1:200, Life technologies) for 1 h diluted in PBS containing 4 % BSA and 1 % goat serum at RT in a humidified dark chamber ...
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bioRxiv - Cell Biology 2023Quote: ... 0.05% Tween-20 in PBS along with 1:1000 To-Pro-3 (Invitrogen) for 2h at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... species-specific secondary antibodies (Life Technologies; 1:300 dilution in 3% BSA/PBS). Images were collected on a Zeiss Axioimager.D2 upright microscope using a 63x/Plan-neofluar objective and captured using a Coolsnap HQ2 camera (Photometrics ...
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bioRxiv - Biophysics 2023Quote: ... NucGreen™ Dead 488 (Invitrogen™, 1 drop per 3 ml in PBS); Alexa 647 Phalloidin (Invitrogen™ at 1/500) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 U/ml EPO (Zyrop 4000 IU injection) and 1× Pen-Strep (Gibco) as per established protocols ...
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bioRxiv - Cell Biology 2023Quote: ... INS-1 832/3 cells were transfected with 40 nM of Control (Ambion Silencer Select Negative Control #1 siRNA ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were maintained in E-medium (3:1 ratio of DMEM:F12 (Thermofisher, 21765029) medium supplemented with 10 % FBS and 1 % PS ...
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bioRxiv - Bioengineering 2022Quote: Primary neonatal cardiomyocytes were isolated from C57BL/6 mouse neonates on postnatal days 3-5 using the Pierce Primary Cardiomyocyte Isolation Kit (Thermo Fisher) as previously described (14) ...
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bioRxiv - Developmental Biology 2019Quote: ... blastocysts were collected from uterine horns and put on culture for 3-6 days in ES derivation medium composed of GlutaMAX/DMEM (Gibco, 31966), 15% FBS (Biowest) ...
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bioRxiv - Cell Biology 2019Quote: The staining of limiting membrane of the yeast vacuole was achieved with FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Invitrogen). Cells were grown to early-log phase in appropriate medium ...
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bioRxiv - Systems Biology 2019Quote: A mate-pair library with 3-6 kb fragments was prepared from pure genomic DNA using Ion TrueMate Library Reagents (Life Technologies). The Ion PGM™ template OT2 400 kit (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Embedded tissues were cut into 3-6 µm sections using a paraffin microtome (Leitz) and placed on SuperFrost® Plus slides (Menzel Gläser, Thermo Scientific). Deparaffination and antigen retrieval was done as described previously (Link et al. ...
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bioRxiv - Neuroscience 2020Quote: ... All 27 samples from 3 groups and 6 GIS were divided and labeled using two sets of 5 mg 11 plex TMT reagents (Thermo Scientific A34808 ...
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bioRxiv - Immunology 2022Quote: ... and the wells were washed five times with wash buffer before the addition of 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS, #37615, Thermo Fisher Scientific). Optical density was read 40 min later at 405 nm using a plate reader (SpectraMax i3 ...
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bioRxiv - Cell Biology 2021Quote: ... The same amount of protein lysate was aliquoted into different PCR tubes and simultaneously subjected to 6 different temperatures (37, 41, 45, 49, 53, 57 °C) for 3 min in the Veriti Thermal Cycler (Applied Biosystems). After 3 min cooling on ice ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were expanded to 6 × 106/mL the day before transfection and were then diluted to 3 × 106/mL by ExpiCHO™ expression medium (Gibco). Plasmids were diluted to 20 μg/mL by cold OptiPRO medium (Gibco ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was added to cDNA and the DNA was denatured at 95°C for 3 min prior to loading in Novex 6% TBE-urea gel (Thermo Fisher). Samples were separated for 45 min at 180 V ...
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bioRxiv - Neuroscience 2022Quote: ... 3.5 cm) containing 30 larvae and a plastic-covered stirrer (magnetic stir bar micro PTFE 6 mm x 3 mm; Fisher Scientific) were placed on a magnetic stir plate (Variomag Poly 15 stirrer plate ...
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bioRxiv - Biochemistry 2023Quote: 1.3 million HEK293T cells were seeded in a 6 cm dish in 5 ml of DMEM [high glucose DMEM (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Neuroscience 2024Quote: Determination of sIL-6R was performed in the FN of NL and 21 dpi of aged WT and GFAP-IL6Tg (n=3-6/experimental group) using a precoated mouse ELISA kit (EMIL6RA, Fisher Scientific) and following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... hansenii cells were concentrated 10x in 200 μL YPD containing 40 μM FM4-64 dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) dye (Invitrogen™) to label endosomes for 8 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 µL of primers (final concentration 200 nM) and 6 µL of Power SYBR Green PCR Master Mix (Thermo Fisher Scientific). The amplification conditions included an initial incubation for 2 min at 50°C ...
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bioRxiv - Neuroscience 2023Quote: HEK-293T cells were seeded on 6-well culture plates at 3 x 106 cells/well and grown in Dulbecco modified Eagle medium (DMEM; Thermo Fisher Scientific ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... NM_002524.5) with a 3’ terminal 6 × His-tag was chemically synthesized and cloned into the pFastBac1 vector (Invitrogen, Carlsbad, California, USA). cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc. ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were subjected to 6 rounds of 10 sec sonication each at power 3 in a 550 Sonic Dismembrator (Fisher Scientific). Between each round of sonication ...
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bioRxiv - Genomics 2024Quote: ... Resulting mRNA was split into 6 separate reactions and cDNA was synthesized using a gene-specific primer (5’-CTCATCAATGTATCTTATCATGTCTG-3’) and MultiScribe Reverse Transcriptase (Invitrogen, 4311235). Following cDNA synthesis ...
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bioRxiv - Neuroscience 2024Quote: ... differentiated OLs at 6 DIV (‘OL 3 days’) were transfected with plasmids using Lipofectamine LTX with Plus Reagent (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: The interaction between PAR-2 and GSP-1/-2 was assessed using a GAL4-based system (Gateway, Invitrogen) using the MAV203 yeast strain ...
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Nanoscale molecular architecture controls calcium diffusion and ER replenishment in dendritic spinesbioRxiv - Neuroscience 2021Quote: Cultures were incubated for 1 h in Fluo-2 (high affinity) AM (2 μm; Invitrogen, Carlsbad, CA, USA) containing recording medium containing (in mM ...
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bioRxiv - Microbiology 2022Quote: ... cells were grown in NB medium with Fe0 granules (2 g;1 to 2 mm diameter; Thermo Scientific) as the sole electron donor ...
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bioRxiv - Cell Biology 2023Quote: ... eyes were moved to 2:1 Cryomatrix:30% sucrose for 2 hours and embedded in Cryomatrix (Fisher Scientific). Sections were collected on a Leica cryostat using charged Histobond slides (VWR ...