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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for TNF RII Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... anti-mouse (Life Technologies, A11020); Alexa Fluor 594 ...
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bioRxiv - Neuroscience 2024Quote: ... anti-mouse (Life Technologies, A11029); Alexa Fluor 488 ...
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bioRxiv - Microbiology 2024Quote: ... Primary mouse anti-nucleocapsid (Thermofisher) was diluted 1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were probed with mouse anti-HA (12CA5) and mouse anti-Pgk1 (22C5D8, Molecular Probes), followed by probing with mouse anti-Pgk1 (22C5D8 ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse primary antibody diluted 1:10,000 and anti-mouse-HRP secondary antibody (Thermo Fisher Scientific) diluted 1:50,000.
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 555 Mouse and Alexa Fluor 488 Mouse secondary antibodies were obtained from Invitrogen. The polyclonal antiserum to the recombinant human megalin cytoplasmic domain (anti-megT ...
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bioRxiv - Microbiology 2022Quote: ... anti-mouse IgG/IgM AlexaFluor 488 and anti-mouse IgG/IgG2α AlexaFluor 546 (both Invitrogen) were used ...
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bioRxiv - Immunology 2022Quote: ... and anti-pp65 antibody (mouse, Argene) combined with goat anti-mouse antibody (AF488, Thermo Fisher), respectively.
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bioRxiv - Genetics 2020Quote: ... cells were incubated in secondary antibodies Mouse Texas Red X (ThermoFisher, anti-mouse, 1:500) and Cy5 (anti-rabbit,1:500) ...
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bioRxiv - Immunology 2020Quote: Mouse serum or plasma was assessed via the IgG (Total) Mouse ELISA Kit (Thermo Fisher), according to the manufacturer’s directions ...
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bioRxiv - Immunology 2022Quote: ... mouse-anti-mouse/human/rat monoclonal PP1a (1:1000 dilution, number MA5-17239, ThermoFisher Scientific), rabbit-anti-Y.pestis polyclonal YopM (1:1000 dilution ...
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bioRxiv - Immunology 2023Quote: ... in PBS1X containing MOM reagent (ReadyProbes™ Mouse-on-Mouse IgG Blocking Solution, Invitrogen, R37621) at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor 350 goat anti-mouse and Alexa Fluor 488 goat anti-mouse IgG (Invitrogen).
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bioRxiv - Immunology 2024Quote: ... and mouse monoclonal antibody to mouse IFNβ (Clone: MAR1-5A3) were obtained from Thermo Scientific. BHA (Cat# B1253 ...
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bioRxiv - Cell Biology 2023Quote: ... Alex Fluor 647 goat anti-mouse and Alexa Fluor 594 goat anti-mouse from Invitrogen. All secondary antibodies were used at a concentration of 1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal anti 6xHis (mouse monoclonal, HIS.H8, Thermo Fisher Scientific, MA1-21315; WB 1:2000), anti GFP (mouse monoclonal ...
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bioRxiv - Genomics 2023Quote: ... EY.T434 mouse embryonic fibroblast cells mouse embryonic fibroblast cells were grown in DMEM (ThermoFisher 10566016) supplemented with 15% (vol/vol ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa 594 goat anti-mouse/rat (1:1000, Invitrogen A-11032 mouse, A-11007 rat) and DAPI (1:500 ...
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bioRxiv - Plant Biology 2019Quote: ... Aliquots were analyzed on a 10% SDS-PAGE followed by Coomassie Blue staining or Western blot analysis with goat α-6X-His-HRP antibody (Life Technologies, Carlsbad, CA) at a 1:5,000 dilution in Tris-buffered saline (20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The gene optimized for the Drosophila codon usage and extended by C-terminal His-tag was purchased from Life Technologies (DNA String fragments, USA). The DNA fragment was inserted into a pMT-BiP-V5-His_A vector (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: 1-2 μg of purified 6xHis-tagged calcineurin or 6xHis-MBP as a negative control was first bound to 10 μL magnetic Dynabeads™ His-Tag Isolation and Pulldown Beads (Thermo Fisher Sci., 10104D) in 490 μL base buffer (50 mM Tris-HCl pH 7.5,150 mM NaCl ...
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bioRxiv - Plant Biology 2019Quote: ... 1 μL of each reaction was added to a mix containing 9 μL of Hi-Di Formamide (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) and 0.5 μL of GeneScan 500 LIZ Size Standard (Applied Biosystems ...
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bioRxiv - Plant Biology 2019Quote: ... 1 μL of each reaction was added to a mix containing 9 μL of Hi-Di Formamide (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) and 0.5 μL of GeneScan 500 LIZ Size Standard (Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by sequencing using the Ion PI™ Hi-Q™ Sequencing 200 Kit on Ion Proton PI™ V2 chips (Thermo Fisher Scientific), an Ion Proton™ System ...
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bioRxiv - Genetics 2020Quote: ... in all the above clonings except for the his-72::GFP repair template which required ccdB resistant bacteria for which we used One Shot ccdB Survival (Thermo Fisher Scientific, cat. A10460). DNA extractions by miniprep were done with the ZymoPURE Plasmid Miniprep kit and elution with water (Zymo Research ...
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bioRxiv - Immunology 2020Quote: ... Nunc MaxiSorp ELISA plates were coated with 50 μL of capture antibody (anti-6x-His antibody clone HIS.H8; 2 μg/mL; Thermo Fisher Scientific, Waltham, MA, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2024Quote: ... BMDMs were plated a day in advance on a coverslip lined 24-well plate at 8 x105 cells in 1mL per well, in “BMDM media” supplemented with 10% L929 (20% HI FBS, 1% penicillin-streptomycin, 1% non-essential amino acids (ThermoFisher, cat#11-140-050), 1% sodium pyruvate) ...
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bioRxiv - Microbiology 2024Quote: ... The amplified PCR product of the C-His oipA gene fragment was cloned into vector pLATE11 using aLICator LIC Cloning and Expression Kit 1 (Thermo Fisher Scientific, United States) by following the kit’s instructions ...
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bioRxiv - Pathology 2023Quote: ... The cDNA was used for RT-qPCR with qPCRBIO SyGreen Blue mix Hi-ROX (PCR Biosystems, London, UK) on the StepOne Plus Real-Time PCR machine (Applied Biosystems, Foster City, CA) and primers listed in Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... Protein bands were visualized with Coomassie staining (ROTIBlue Quick, Carl Roth) or immunodetected with a monoclonal anti-His antibody conjugated to HRP (MA1-21315-HRP, Thermo Fisher, 1:2000 diluted). A detailed protocol can be found in protocols.io (dx.doi.org/10.17504/protocols.io.bu67nzhn).
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bioRxiv - Genomics 2023Quote: ... Vero/hSLAM and VeroE6/TMPRSS2 were maintained in supplemented DMEM with 10% HI-FBS and 1 mg/mL G418 sulfate (Thermo Fisher 10-131-027). HeLa/ACE2/TMPRSS2 cells were a generous gift from Dr ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% (v/v) heat-inactivated (HI) FBS (Thermo Fisher Scientific, Waltham, MA, USA). Stable cell lines expressing F-CIC-S ...
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bioRxiv - Neuroscience 2021Quote: ... They were then rinsed in block for >30 min at room temperature before incubation in secondary antibody (1:250 Invitrogen #A21-42 AF488 anti-mouse IgM; 1:250 Life Technologies #A11029 AF488 anti-mouse IgG1; 1:250 Invitrogen #A21141 AF488 anti-mouse IgG2b) in blocking solution for > 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and secondary antibody (anti-mouse IgG2a AF647, 1:2000, Invitrogen, A-21241; anti-mouse IgG AF568, 1:2000, Invitrogen, A-11031; anti-mouse Cy5, 1:1000), sequentially ...
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bioRxiv - Neuroscience 2019Quote: ... Alexa Fluor 488 anti-mouse IgG or Alexa Fluor 568 anti-mouse IgG (Thermo Fisher Scientific) at 1:500 for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... mouse macrophage RAW 264.7 cells and mouse myoblast C2C12 cells were cultured in DMEM medium (Gibco); colorectal carcinoma COLO 741 cells and B-CLL MEC1 cells were cultured in RPMI 1640 medium (Gibco) ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse cytokines were measured using a mouse cytokine 10-Plex Panel kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-mouse Alexa A647 (1:1000; goat anti-mouse IgG Alexa Fluor 647; Invitrogen #A21236) for 2h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-CLIMP63 (followed by anti-Rabbit/Mouse/goat Alexa-dye coupled antibodies (1:400, Invitrogen). SiR-lysosome was used to visualize lysosomes in live microscopy (1:2000 ...
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bioRxiv - Immunology 2020Quote: ... Goat anti-Mouse-(H&L)-Alexa488 and Goat anti-Mouse-(H&L)-Alexa647 secondary antibodies (Invitrogen), Alexa488-conjugated phalloidin (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated in 1:1000 anti-mouse AlexaFluor488 or anti-mouse AlexaFluor594 (Thermo Fisher Scientific) and 4’,6-diamidino-2-phenylindole counterstain (DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... TMEM119 (mouse primary, Biolegend, 853302, 1:250, donkey anti-mouse 488 Life Technologies, A21202, 1:1000), and CX3CR1 (PE-rat ...
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bioRxiv - Immunology 2022Quote: ... WT mouse CD8+ T cells were activated with anti-mouse CD3/CD28 dynabeads (11453D, Thermo Scientific) at a 1:1 cell-to-bead ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... and subsequently with fluorophore-labeled anti-mouse (goat anti-mouse Alexa Fluor 488; 1:2000; Invitrogen) and anti-rabbit secondary antibodies (goat anti-rabbit Alexa Fluor 594 ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-mouse-IgG2 Alexa 568 and goat anti-mouse-IgG1 Alexa 488 (1:500, Invitrogen).
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bioRxiv - Plant Biology 2024Quote: ... Mouse monoclonal antibodies were detected with goat anti-mouse IgG HRP-linked antibody (61-6520; Invitrogen) diluted 1:5000 (v/v) ...
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bioRxiv - Microbiology 2019Quote: ... Eluted proteins were analyzed by SDS-PAGE followed by Coomassie-staining or western blot using an anti-His primary antibody (Thermo Fisher Scientific, Waltham, MA, USA), HRP-conjugated secondary antibody (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Bioengineering 2021Quote: ... THP-1 cells were expanded in RPMI medium (ATCC® 30-2001™) supplemented with 10% heat inactivated fetal bovine serum (HI-FBS, Gibco, ThermoFisher Scientific, Massachusetts, USA), 100 U/ml penicillin and 100 μg/ml streptomycin sulfate (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... The eluted protein was then incubated with purified 6x-His-TEV protease E106G (Cabrita et al. 2007) and 500 μM TCEP (Thermo Fisher Scientific, product number 77720) for 20-24 hours ...
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bioRxiv - Microbiology 2021Quote: ... Standard Western blotting procedures were used with the following antibodies: HIV-1 gp120 (NIH AIDS Reagent program catalog no. 288) and 6x-His Tag (ThermoFisher catalog no. MA1-21315-HRP).